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Attacks on active network elements
Ščepko, Richard ; Kacálek, Jan (referee) ; Polívka, Michal (advisor)
The bachelor thesis deals with the topic of the security of computer networks. The tasks of the bachelor thesis are the attacks on active network elements with the aim of the catching of data between two users. In the created structure with a stellate topology, the user stations have connect to the active element (switch). In the thesis, the individual ways of attacks and the significance of ARP proceedings to MAC addresses have describ. Several programmes have use in order to take control over the device. Due to a big amount of these active elements the source code of the programmes had to be alter. The work with the programmes ARPtool and ARPoison demanded the operational system Linux, in our case the distribution of Ubunt. The programme WinArpAttacker could be set off under the system Windows XP as well. The achieved results and the description of the practical part are discuss in detail in the summary of the thesis. The result is the catching communication with the help of the programme WireShark.

The Controlling Study
Herda, Tomáš ; Mikovcová, Hana (advisor) ; Herda, Zdeněk (referee)
The main goal of this Diploma´s Thesis is to make a model for calculation of water and sewer rates for the company Vodovody a kanalizace Náchod, a.s. when sticking to the set criteria both from the side of VaK Náchod, a.s. and law regulations. Based on the theoretical part an analysis of customer sensitivity to the price changes using the data for last 20 years follows. Findings from the first two parts are used in risk analysis in next part. The created model calculates the water and sewer rates based on the information from the company accounting system in the way to generate sufficient financial resources to fulfill the renovation plan of infrastructural property plant and equipment and to transfer given amount to the company funds. In addition, the model monitors whether the legal condition of maximal allowable increase of profit per m3 is met. In the customer sensitivity to the price changes part the price elasticity of demand for water and sewer rates is calculated based on the data from 1995 to 2015. The assumption of inelastic demand is confirmed. Risk analysis part is deals with potential risk regarding the demand and prices. Potential impacts for the most significant risk are quantified. The analysis uses knowledge gained in the first two parts. It was confirmed that potential risks are exiting but do not have any significant impact on the going concern of VaK Náchod, a.s. The created model has been already used for the calculation of prices for the year 2017. Customer sensitivity analysis to the price changes and link to the potential risks is an additional information for VaK Náchod, a.s. which validates that nowadays, there are no significant threats which could affect the demand and water and sewer rates significantly.

Permeation of VOC vapours through carbon nanotube network membranes controlled electrically
Slobodian, P. ; Říha, Pavel ; Olejník, R.
In this letter, we report the increase of permeation rates of some typical volatile organic compounds through entangled carbon nanotube networks by an electric current. The change in the permeation rate is reversible when the\ncurrent is turned off. The permeation rise is partly probably due to Joule effect and thus increased membrane temperature and vapor pressure in the vicinity of the inlet side of membrane. However, the effects of vapor polarity and electrostatic interaction of vapors and charged nanotubes seem to be also involved and contribute to the differentiation between alcohol and carbohydrate vapors permeation.

Modelling, parameter estimation, optimisation and control of transport and reaction processes in bioreactors.
ŠTUMBAUER, Václav
With the significant potential of microalgae as a major biofuel source of the future, a considerable scientific attention is attracted towards the field of biotechnology and bioprocess engineering. Nevertheless the current photobioreactor (PBR) design methods are still too empirical. With this work I would like to promote the idea of designing a production system, such as a PBR, completely \emph{in silico}, thus allowing for the in silico optimization and optimal control determination. The thesis deals with the PBR modeling and simulation. It addresses two crucial issues in the current state-of-the-art PBR modeling. The first issue relevant to the deficiency of the currently available models - the incorrect or insufficient treatment of either the transport process modeling, the reaction modeling or the coupling between these two models. A correct treatment of both the transport and the reaction phenomena is proposed in the thesis - in the form of a unified modeling framework consisting of three interconnected parts - (i) the state system, (ii) the fluid-dynamic model and (iii) optimal control determination. The proposed model structure allows prediction of the PBR performance with respect to the modelled PBR size, geometry, operating conditions or a particular microalgae strain. The proposed unified modeling approach is applied to the case of the Couette-Taylor photobioreactor (CTBR) where it is used for the optimal control solution. The PBR represents a complex multiscale problem and especially in the case of the production scale systems, the associated computational costs are paramount. This is the second crucial issue addressed in the thesis. With respect to the computational complexity, the fluid dynamics simulation is the most costly part of the PBR simulation. To model the fluid flow with the classical CFD (Computational Fluid Dynamics) methods inside a production scale PBR leads to an enormous grid size. This usually requires a parallel implementation of the solver but in the parallelization of the classical methods lies another relevant issue - that of the amount of data the individual nodes must interchange with each other. The thesis addresses the performance relevant issues by proposing and evaluation alternative approaches to the fluid flow simulation. These approaches are more suitable to the parallel implementation than the classical methods because of their rather local character in comparison to the classical methods - namely the Lattice Boltzmann Method (LBM) for fluid flow, which is the primary focus of the thesis in this regard and alternatively also the discrete random walk based method (DRW). As the outcome of the thesis I have developed and validated a new Lagrangian general modeling approach to the transport and reaction processes in PBR - a framework based on the Lattice Boltzmann method (LBM) and the model of the Photosynthetic Factory (PSF) that models correctly the transport and reaction processes and their coupling. Further I have implemented a software prototype based on the proposed modeling approach and validated this prototype on the case of the Coutte-Taylor PBR. I have also demonstrated that the modeling approach has a significant potential from the computational costs point of view by implementing and validating the software prototype on the parallel architecture of CUDA (Compute Unified Device Architecture). The current parallel implementation is approximately 20 times faster than the unparallized one and decreases thus significantly the iteration cycle of the PBR design process.

Use of inhalation systems with asthma in connection with nursing
KLEINOVÁ, Irena
Asthma bronchiale is a chronic inflammatory disease of the airways, connected to bronchial hyperactivity leading to shortness of breath, wheezing, chest tightness and cough. It effects people of all age categories and cannot be efficiently cured nor prevented. The incidence ranks this disease among the most common children´s chronic diseases. It affects a significant part of the global population - records say about 300 million people. Its prevalence has dramatically increased over the last 20 years. It is estimated that in 2025 about 400 million people will suffer from asthma. Cornerstones of asthma control are based on early diagnostics, efficient treatment, adherence to lifestyle changes and proper use of inhalation devices. Once the treatment is effective and check-ups and the use of inhalation therapy are regular, the prognosis of asthma is very favourable and it is possible to improve the patient´s quality of life. The control over asthma and its maintenance is the main objective of treatment. This can be achieved by using anti-inflammatory therapy, especially in inhalation form. This Bachelor´s thesis focuses on mapping inhalation systems with patients with asthma bronchiale. To a large extent it deals with the issue of use of inhalation systems, keeping to lifestyle changes and the role of the nurse in asthma-specialized outpatient´s departments. We see the relevance of this topic in the fact that, despite all available literature and medical advice, many asthmatics still make mistakes in the inhalation technique. Wrongly selected inhalation systems or wrongly selected inhalation techniques results in therapeutic failures and problems of patients get worse. This does not maintain control over asthma. The fact that many asthmatics do not know their exact diagnosis and underestimate some symptoms is very alarming. In the introduction, the theoretical part focuses on the definition of the disease. Other chapters include causes and symptoms of asthma, its classification, diagnostics and treatment. It also provides information about the treatment of acute asthma attacks - exacerbation and the role of the nurse in examination of an asthmatic patient. A great part of the thesis deals with inhalation systems, their types and effects. The preparation for the use of manual handheld inhalers and nursing care for the asthmatic patient in outpatient and inpatient care is developed at the end of the theoretical part. The objective of this part was to summarize the current knowledge about this issue. The objective of the Bachelor´s thesis was to map the use of inhalation systems in patients with asthma bronchiale. The following research questions were used: How do nurses inform patients about the use of inhalation systems? What type of inhaler do patients like? What is the nurse´s involvement in the examination of asthmatic patients? What are the most common mistakes in the application with an inhaler? How do asthmatics keep to lifestyle changes? The practical part of the thesis contains the results of qualitative research which was made on the basis of the semi-structured interview. Final interviews were transcribed and subsequently processed by the technique of open coding. The research sample was made of 12 asthmatics and 9 nurses working in selected asthma-specialized outpatient´s departments. With respect to the results found, an information brochure was created - see Annex 7 - which is intended for use by asthmatics. We believe that the results of this thesis will be helpful for asthmatics and increase the interest of experts in this issue and will also address other people willing to take part in its solution.

Fluidized-bed Combustion of Dry Stabilized Sewage Sludge in Oxy-fuel Mode
Moško, Jaroslav ; Pohořelý, Michael ; Durda, Tomáš ; Zach, Boleslav ; Šyc, Michal ; Svoboda, Karel
Sewage sludge is an inevitable waste stream from wastewater treatment and its disposal is complicated due to specific characteristics of its composition. Sewage sludge combustion/incineration is the only suitable way for disposal of large volumes of sewage sludge containing POPs and some heavy metals. Sewage sludge combustion reduces the volume of such waste and causes destruction of organic contaminants. Oxy-fuel combustion is a technology for simplifying CO2 sequestration from the flue gas by using nearly pure oxygen instead of air for the combustion. In order to control the combustion temperature, part of the flue gas is recycled to combustion chamber. The paper brings information about investigation of the effects of combustion temperature and concentration of oxygen in inlet combustion media on the emissions of NOx, N2O and SO2 in FB combustion of sewage sludge. The experimental results have shown that an increase in combustion temperature leads to an increase in NOx and to a decrease in N2O emissions in the temperature range 750–930 °C. The lowest SO2 emissions were measured in the temperature interval from 800 to 820 °C in all experiments with different oxygen concentration in inlet gas. It was found that when the oxygen concentration in inlet gas increased, the emissions of SO2 increased while emissions of NOx slightly decreased.
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Analysis breeding of milk cattle on the chosen biofarme
Plášková, Pavlína ; Toušová, Renata (advisor) ; Ducháček, Jaromír (referee)
The aim of this thesis is to evaluace the conditions of dairy cattle breeding in ecological agriculture and to compare them with concrete values taken from the chosen ecological farm Bílčice, which has been part of ecological agriculture since 2011 and which is focused on czech fleckvieh breeding and bio milk production. The first part deals with dairy cattle breed characteristic, main objectives and ecological agriculture principals, welfare problems, breed cattle and utility control, housing technology, nutrition and feeding, mechanical milking technology, milk production, reproduction, health condition and breeding economy. The practical part contains the rating of milk yield index (milk production, % proteins, % fat) and reproduction index ( meantime, perioda service, insemination index and after first parturition percentage). 650 pieces of cos were stabled at farm, from that 200 pices of dairy cos were mostly czech fleckvieh. Monitoring was made in 2013. Dayily milk production was around 2 750 kg. The milk utility for lactacion was in 2013 6 100 kg of milk, 3,89 % fat and 3,34 % proteins. The dairy cos were free stabled with high bedding, They were mechanically milked twice a day in herringbone parlours. Milk is being delivered once in twodays to Olma dairy. Cows are regularly driven out to pasture in summer feeding period. They are extra fed with 10 kg of clover silage and 6 kg of scarp (barely, wheat, triticale). Cows stays in stables for winter time. The ration consists of 45 kg of clover silage, 6 kg of scarp, 2 kg of lupine and 1 kg of corn in first time of lactacion. The cow utility in transitional form of economy at farm Bilcice was 7 477 kg of milk in 2010, a value in the Czech Republic was 7 726 kg of milk. After entering the ecological agriculture in 2013 milk yield at the farm decreased on 6 100 kg of milk and the average value in the Czech Republic increased to 8 370 kg of milk. The cow utility in transitional form was 4,03 % fat in 2010 and 3,89 % in 2013 (ecological agriculture). A diference between 2010 and 2013 is 0,14 %. Proteins were 3,39 % in 2010 and 3,34 % in 2013. There was average value for every reproduction index. The service period was 96 days and the meantime was 400 days, the insemination index was 1,8 and the after first parturition percentage was 54,2 %.

Information content in vocalization of guinea pigs (Cavia porcellus): individual distinction and recognition of predators
Baklová, Aneta ; Baranyiová, Eva (advisor) ; František, František (referee)
Guinea pigs represent domesticated precocious rodents which became common pets. From the first day after birth they are fully vocal. This thesis was devoted to the vocalization of pups. The aim of the thesis was to 1) determine the age when the vocal individuality in whistle sound is demonstrable; 2) test possible ultrasonic signals emitted by young guinea pigs; 3) study the antipredator reactions and alarm calls to aerial (bird of prey) and terrestrial (dog) predators and human (control test). A total of 16 guinea pig pups were tested for vocal individuality, 28 pups for ultrasonic vocalization and 27 adolescents for predator recognition. When testing vocal ontogeny during the first 9 days after birth, I observed changes in temporal, frequency and parameter of intensity . When I test vocal individuality by cross-validated discriminant function analysis (DFA) based on ten acoustic parameters, calls were classified to correct animals with following success: day 1 = 71.9%, day 3 = 58.8%, day 5 = 53.10%, day 7 = 50.60% and day 9 = 63.10%. The highest frequency in whistle was 30.03 kHz. In predator discrimination reactions as freezing, fleeing and vigilance were observed. In the presence of dog, guinea pigs reacted for the longest time and most frequently by freezing. When confronted with a bird of prey, I observed for a longest time and most often fleeing and then freezing. When exposed to a human, guinea pigs showed mostly vigilance. Almost no vocalization was observed except for two events of alarm calls - drrr as in the presence of dog and chirrup as reaction to bird of prey. The following conclusions can be drawn from the presented results: 1) vocal individuality of guinea pigs is demonstrable immediately after birth and the rate of individually different vocal parameters changes with age; 2) guinea pigs are able to produce sound up to 30 kHz, i.e. within the ultrasound range, but signals of high frequencies are not crucial for their communication; 3) guinea pigs discriminate between terrestrial and aerial predators, but they emit alarm calls rather rarely.

Freezing technology of bull sperm in relation to its survivability and fertilization ability
Doležalová, Martina ; Stádník, Luděk (advisor) ; Jiří, Jiří (referee)
The aim of optimalization the insemination doses production is to provide the highest fertilization ability of spermatozoa during the demanding proces of processing fresh semen and its subsequent cryopreservation. Temperature changes causes spermatozoa damage during the cooling and freezing. Spermatozoa is exposed to cold shock and many others limiting factors, which leads to cell death and therefore to decline of fertilization ability of thawed insemination doses. For increasing spermatozoa resistance, exactly the plasma membrane resistance against cold shock was fraction of egg yolk LDL cholesterol (low density lipoprotein) at various concentrations into the comercially produced diluents added. It is believed that LDL acts possitively to plasma membrane and helps to maintain the fertilization ability of spermatozoa after thawing. Following step in the proces of insemination doses production is slow cooling of diluted semen and equilibration, when the straws are store at cooling box for 30 minutes to 240 hours. This period is necessary to penetrate of certain diluent components into the spermatazoa also maintain the balance between their intracellular and extracellular concentration. Also important is subsequent freezing temperature gradient of insemination doses. The most suitable freezing method is based on computer controlled temperature decline in freezing chamber which allows the precise control of ice crystals formation that could tear and kill the cell. During 2012 to 2016 was repeatedly collected semen from the group of breeding bulls (n = 27, Holstein and Czech Fleckvieh breed) at AI centre. Semen which fulfill the standard entrance conditions in first step was evenly into several parts divided. For dilution the three types of comercially diluents AndroMed, Bioxcell and Triladyl with and without LDL addition were used. Into the diluents AndroMed and Bioxcell the concentration of LDL 4 %, 6 % and 8% into the dilent Triladyl 6 %, 8 % and 10 % was added. Diluted semen was filled into the glass capillares with volume 0,1 ml and temperature +4 °C. Subsequently the sample was placed to cold bath (0°C) for 10 minutes. Then the volume of capillare with physiological solution (37 °C) was mixed and for next 120 minutes was incubate. The effect of cold shock to proportion of live spermatozoa was evaluated by using Eosin and Nigrosine staining technique during heat test of spermatozoa survivability after spermatozoa heating and after 120 minutes of incubation. The more suitable semen diluents which provide the higher spermatozoa resistance against cold shock were AndroMed and Bioxcell. Together the possitive effect of LDL addition into the diluents to lower decrease of proportion of live spermatozoa during heat test was found (P<0.05). The most suitable LDL concentration which had a favorable influence at spermatozoa resistance against cold shock was 6 % in diluent Bioxcell. Values of the proportion of live sperm were higher at the beginning of the heat test (+1.31% to + 3.2%) and after 120 minute incubation (+5.82% to +8.41%) compared to other diluents with and without addition of LDL. In the next step the process of equilibration was optimized, is an important part of insemination doses production. The effect of the length of equilibration for subsequent fertilization ability of spermatozoa was evaluated using spermatozoa motility based of CASA and proportion of live spermatozoa after thawing and during heat survival test lasting 120 minutes (37 ° C). Suitable semen was diluted by comercially used diluent AndroMed based on soya lecithin, filled into the straws (0.25 ml), cooled and equilibrated in cooling box for 30, 120 and 240 minutes and freezed in programmable freezing box applying four types of freezing curves differing in temperature rate decline. There was used standard and by producer recommended 3. phase freezing curve, then 2. phase freezing curve, and 3. phase freezing curve with slower as well as rapid decline of temperature rate in freezing chamber, compared with standard freezing curve. The highest spermatozoa motility was found using 240 minutes of equilibration by +2.72% and +4.58% compared to other lengths of equilibration (P <0.05 to 0.01). The highest proportion of live spermatozoa was found using 120 minutes of equilibration (+6.87 % and +8.68 %). The highest average spermatozoa motility during heat test after thawing was achieved by using 2. phase freezing curve (from +2.97% to +10.37%, P <0.05), also in the proportion of live spermatozoa (from + 4.37% to +8.82%, P <0.01). When evaluating interaction between the length of equilibration and freezing curve (standard 3. phase and 2 . phase freezing curve), the highest average spermatozoa motility and proportion of live spermatozoa using 240 minutes of equilibration by both freezing curves was reached, there was no statistically significant differences. As well as, in all evaluated parts of this study the individual differences between ejaculate of bulls and within semen from one bull (P <0.05) as secondary effect were found. To maintain good fertilization ability of semen during cryopreservation is necessary to increase the spermatozoa resistance against cold shock using addition of correct concentration of LDL into the commercially used diluents AndroMed and Bioxcell. Subsequently the fertilization ability of insemination dose is influenced by cooling, the length of equilibration and freezing. The length of equilibration 120 minutes and more as well as gentle way of freezing according to freezing curve, which ensures a gradual decrease of temperature in freezing chamber provided the higher average spermatozoa motility and proportion of live spermatozoa.