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Determination of zearalenone in malting barley
Wawroszová, Simona ; Čumová,, Martina (referee) ; Běláková, Sylvie (advisor)
This bachelor thesis deals with monitoring of zearalenone content in malting barley using immunochemical method ELISA and consequently method of liquid chromatography with mass spectrometry (LC-MS/MS). Theoretical part describes the brewing raw materials and important toxinogenic filamentous fungi. Special attention was drawn to selected fusarium mycotoxins (fumonisins, trichothecenes, zearalenone). It also describes some methods for determination of mycotoxins. Experimental section describes analyze 90 samples of malting barley of different varieties using method ELISA. In 19 samples zearalenone concentration was higher than limit of quantification. The highest level of zearalenone concentration (39,2 g•kg-1) contained barley, variety Wintmalt, where corn was used as a fore-crop. The samples were subsequently analyzed by LC-MS/MS. Zearalenone concentration was higher than limit of quantification only in four samples, namely in samples of Blaník, Malz and Wintmalt varieties of barley. Furthermore, the samples of intermediate products of malting process were analyzed by LC-MS/MS. No sample showed level of zearalenone higher than limit of quantification.
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Changeof phenolic acids content during malting of spring barley
Štýblová, Romana ; Janoušková, Eva (referee) ; Běláková, Sylvie (advisor)
This bachelor thesis deals with detection of chosen phenolic acids (sinapic acid, caffeic acid, ferulic acid, syringic acid, p – hydroxybenzoic acid, vanillic acid and p – coumaric acid) in the malting barley and monitoring of their changes during making a malt in real enviroment of floor malt house. Tehoretical part describes individual phenolic acids, their presentation in raw material for making of malt and methods for determination . There is described technology of making a malt. The nine samples was taken in experimental part. There were a malting barley, intermediate products of malting, malt and rootlets. Phenolic acids were extracted by alkaline and acid hydrolysis. This samples were detected by Ultra high – performance liquid chromatography using of UV detection (UPLC/PDA).
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Spiral conveyor
Nekoksa, Pavel ; Kašpárek, Jaroslav (referee) ; Malášek, Jiří (advisor)
This bachelor thesis deals with the design and construction design spiral conveyor. In the text part is performed design calculation of the major parts of spiral screw conveyor, drive unit design, storage worm screw, strength check of functional parts and an appropriate choice of material transported. Design documentation based on text part and consists of a conveyor assembly drawing, a list of items and drawing worm screw.
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Determination of ergosterol content in brewing materials
Matyáš, Martin ; Ing.Pavla Kožušníková, Ph.D. (referee) ; Benešová, Karolína (advisor)
Presented bachelor’s thesis is focused on determination of ergosterol content in barley and malt. In theoretical part the presence of ergosterol in barley and malt has been elaborated, the purposes of its determination, detailed overview of previously applied methods of extraction from samples of barley and malt as well as other biological materials and methods of quantification. Practical part is focused on optimizing of extraction and determination of ergosterol by high performance liquid chromatography with diode array detection (HPLC – DAD).
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Vývoj rychlé metody pro stanovení barvy speciálních sladů
Křepelová, Simona
The aim of this work was to suggest a quicker, technically-undemanding, and easy to use method for measuring the malt colour without congress mashing, and to evaluate the extent how the colour values obtained by this quick method will vary from the values obtained by a standard method. 12 malt samples were used for the experiment (3.1 -- 1320 j. EBC), wort was prepared from them in the standard (congress) way and in the new quicker way. The quick method for wort preparation was based on the extraction of overground malt in hot water. The extraction lasted 5, 10, and 15 minutes. The following properties of wort were measured by the spectrophotometer: clarity (L*) a* and b*. Overall differences in the clarity (L*) and calculated total colour difference (delta E*) between the wort prepared by the tested method and the congress wort were caused by the fact that boiling water, which created conditions for Maillard reactions, was used for the extraction. High-molecular colour components started to be formed in the extract. The suggested method seems to be applicable, but it will be necessary to optimize its conditions, especially to decrease the water temperature for extraction.
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Využití speciálních sladů a úpravy varného procesu ve výrobě piva
Ratiborská, Silvie
The theoretical part of the thesis is focused on manufacture of malt which contains income and cleaning of grain, steeping, germination and kilning. Different kinds of malts and their application in the manufacture of beer are described in the work. It also consists of surrogates used in the manufacture of beer. The thesis also deals with brew-ing, wort and beer production. There is a practical part aiming at the production of three variants of purple wheat malt and beer brewing made from wheat malts
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Sledování vybraných parametrů ovlivňujících gushing u sladovnického ječmene
Němec, David
Presented diploma thesis is about malting barley as a initial ingredient for producing beer and about its negative effect called gushing. In the first part of dissertation is described technological procedure of malt production and defined term gushing. Also there are mentioned information about cause of initiation, reduction possibilities and methods to describe gushing phenomena. Practical part is mainly focused to define susceptibility of malting barley to gushing and for testing was chosen three days test Carlsberg. The gushing was monitored on differently treated vegetation of spring barley which was grown in seasons 2012 and 2013 in Hrubčice locality. On the performed 160 testing samples were evaluated influence of threads barley on degree of barley gushing.
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Porovnanie kanditného a pekarského sladového výťažku na pekarské vlastnosti múky a ich využitie v pekarských výrobkoch
Čamajová, Mária
The aim of the thesis with topic ,,Comparison of non-diastatic and diastatic malt extracts on baker's quality of flour and their utilization on bakery products" is: make literature research from this area and verify by experiments described advantages of malts extracts in comparison with standard products. The baking experiment was carried out for comparison characteristics of standard products and products with different amount of malt extract. The baking quality of flour was determined with and without malt extract. There were discovered only small differences. Evaluation of common baked goods confirmed the fact, that addition of malt extract raises the volume of product, since all samples, except of sample with addition of 2% non-diastatic malt extract, reached the higher values of specific volume than standard. The results of sensory evaluation were relatively balanced. However, in evaluation of taste of common baked goods all samples with addition of malt extract had more sensory points like standard.
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Možnosti výroby cereálních výrobků z vybraných cereálií
Knotová, Denisa
The theoretical part of the paper concentrates on the application and appropriateness of different cereals for human consumption. The issue of malt production from selected cereals and its subsequent processing into food products are also addressed there. The practical part is focused on the production and processing of selected grains and malts on a particular food product. The practical part is focused on the production and processing of selected grains and malts on a particular food product, which is cereal drink from malted oat and oat. The basic parameters of selected cereals are determined. For drink are set the basic nutritional parameters, it is subjected to organoleptically evaluation and the test of conservation. Another product is a syrup of malt wort made from malted barley, which was organoleptically evaluated.
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Možnosti ovlivnění kvality sladu a jeho uplatnění ve výrobě piva
Podaný, Adam
In the literary part of the thesis are information about the possibilities of influencing the quality of malt and its application in the beer production. In the practical part we explored the effect of nitrogen and sulphur on quality of malt. The results of small-plot field trials established on plots of the Agrospol agricultural enterprise in 2012 in Velká Bystřic we can see the positive effect of applied N and S fertilizers on the quality of the malt produced from the grain of spring barley variety Bojos. Nitrogen and sulfur fertilization improved grain germination. The grain of the variant fertilized with N and N + S - fertilizers had faster germination. Sulfate sulfur increased the starch content in the grain. The amount of extract in malt corresponded with the starch content in barley grain. Also content of the proteins is corresponded with doses of the nitrogen fertilizers.
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