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Characterisation and maturation process of Edam type cheese
Dostálková, Andrea ; Vespalcová, Milena (referee) ; Vítová, Eva (advisor)
This diploma thesis deals with the assessment of aroma compounds and free/bound fatty acids in model samples of Edam type cheeses. Various combinations of microbial cultures, basic mesophilic and four selected strains of thermophilic Lactobacillus casei and Lactobacillus plantarum, were used for cheese production. The main aim was to judge the influence of microbial cultures on content of measured parameters, the changes during ripening were also followed. The HS-SPME-GC-MS method was used for identification and semiquantification of aroma compounds. The lipids from cheese were extracted based on ISO 1735:2004, fatty acids were identified and quantified using GC-FID method after conversion to methyl esters by acid esterification with boron trifluoride in methanol. In total 50 volatile compounds were identified in samples, 11 alcohols, 5 aldehydes, 7 ketones, 6 acids, 10 esters, 3 lactones, 2 sulphur compounds and 6 „others“; all of them are considered as aroma active. About 30–40 compounds were identified in single samples, alcohols, acids and ketones were the predominant. The number as well as the content of compounds identified decreased slightly during ripening. In total 17 free/bound fatty acids were identified in samples, their content also decreased during ripening. Myristic, palmitic, stearic and oleic acids were the predominant. The significant differences (p
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QoS in UMTS
Kavan, Radovan ; Hanus, Stanislav (referee) ; Kejík, Petr (advisor)
This thesis treats of QoS (Quality of Service) in UMTS system. The term quality of service is frequently used, recently, however not only in computer networks, but also more and more in mobile networks also. Present system of 2nd generation (2G) GSM enables only limited exploitation of QoS function. System UMTS is a system of 3rd generation (3G) already and contains quality of service in greater measure which is ensured with number of algorithms and functions. Individual QoS functions enable effective utilization of radio interface, support maintenance of planned coverage and offer high spectral efficiency. Functions that cater to quality of service are called RRM functions (Radio Resource Management). There exist five basic functions in UMTS system that are Admission control (AC), Power control (PC), Load control (LC), Handover control (HC) and Packet scheduler (PS). These functions are responsible for control of network access, control of power, control of network load, control of handover and packet (bit rate) scheduling. Last three functions are usually collectively designated as Congestion control, control of network overload. UMTS layer model, differentiation of services, traffic classes and 3GPP concept are also discussed. In thesis the attention is piad to Admission control algorithm – control of network access – in the uplink direction and to different ways of load (in the cell) modeling. Individual approaches are simulated in MATLAB. A possibility of using algorithm in the real system is discussed in conclusion.
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Proteome and Metabolome Changes in Selected Organisms under Stress
Halienová, Andrea ; Kráčmar, Stanislav (referee) ; Čertík, Milan (referee) ; Šucman, Emanuel (referee) ; Márová, Ivana (advisor)
Living conditions of every organism are influenced by various factors at this time. Some of them have positive effect on organism, some negative. Basic condition for surviving is the ability to resist and adapt to changing metabolic and living conditions. Every single stress effect can lead to changes in metabolism but organisms have ability to develope sufficient mechanisms for stress response. Some of them are similar for all living organisms (enzyme production, endogenous primary stress metabolites) some of them are specific for certain organism or stress type. Cell stress response can be observed on different levels (proteomic, genomic, metabolomic). In proper conditions it can be used indrustrially. In this work, influences of various stress factors were studied. These factors were applied on selected organisms – carotenogenic yeast and plant materials. Yeast stress response was induced by osmotic and oxidation stress factors. Changes on proteomic level and in production of selected secondary metabolites were observed. Proteome was analyzed by 1D and 2D electrophoresis with subsequent analysis of proteins by mass spectrometry. Yeast strain Rhodotorula glutinis CCY 20-2-26 showed the best adaptation to stress factors, which was moreover accompanied by overproduction of carotenoids. This finding can be premise for next industrial production of carotenoids. In plant samples predominantly enzymes and metabolites involved in antioxidant response were studied.
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Mathematical analysis in mass spectrometry
Vlachová Hutová, Eliška ; Adam, Vojtěch (referee) ; Valla, Martin (advisor)
In this bachelor work, there is theoretically described the principle of mass spectometry and tandem mass spectometry, usage the method in practices and description of functionality of separate analysers. Next, there is also mentioned the description of mass spectrum and the algorithm to its analysis. Practical part of the work describes the creation of analytic tool to evaluate spectrum and following usage of this programming tool for the practical analysis of flavonoid compounds, concretely isoflavonoid.
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Kindergarten at the Obilní trh in Brno
Walter, Jaromír ; Cahel, Tomáš (referee) ; Májek, Jan (advisor) ; Bantová, Sylva (advisor)
Subject of my bachelor thesis was creating suitable building for kindergarten in Brno-City, near Obilni Trh square. Given parcel was situated opposive of Obilni Trh's square and was created partially by destruction of buildings during World war 2. Subject building's design consists of areas suited for Kindergarten, areas for renting and underground parking. Building is situated further away from the street line to create optimal setting for kindergarten and to offer space for city services.
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The optimization and validation of the GC-MS method for assessment of volatile aroma compounds in cheese
Koubek, Miroslav ; Babák, Libor (referee) ; Vítová, Eva (advisor)
This work deals with the introducing of the method for the determination of volatile aroma active compounds in different types of natural and processed cheeses. The theoretical part describes the basic groups of aroma compounds. Than the methods used for their determination with focusing on solid phase microextraction and gas chromatography coupled with mass spectrometry are described. The experimental part deals with the optimization of the selected method parameters. For extracting volatile substances from the sample matrix the fiber DVB/CAR/PDMS 50/30 m was selected, these compounds were then determined by gas chromatography coupled with mass spectrometry. In the frame of validating proces the repeatability of the method was verified, RSDs of retention times
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Comparison of aroma profile of goat vs. cow milk
Pospíchalová, Alžběta ; Saláková,, Alena (referee) ; Vítová, Eva (advisor)
This diploma thesis deals with the comparison of aroma compounds in goat and cow milk. The content of aroma active substances in samples of goat and cow milk, which were analyzed by HS-SPME-GC-MS method, is monitored. In the theoretical part, milk is characterized, its formation in the dairy mammary gland is described, its composition including an overview of aromatic active substances, physical and chemical as well as its sensory properties, milk defects and differences between cow's and goat's milk are described. Further, gas chromatography in combination with solid phase microextraction technique in tandem with mass spectrometry is described. In the experimental part, 13 samples of goat and 8 samples of cow milk were subjected to HS-SPME-GC-MS analysis. Samples were analyzed fresh and after heat treatment. Altogether, 78 volatile substances were identified in the samples. Ketones were the most numerous, aldehydes, alcohols and carboxylic acids were the most abundant chemical groups. There were differences between samples in number as well as in content of the identified compounds, 64 in goat milk and 48 in the cow milk.
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School, the Foundation of Life – a Complex of Educational Buildings in Ostrava, Cerna louka
Matušková, Martina ; Nový, Alois (referee) ; Nový, Vítězslav (advisor)
The project concerns itself with a current issue concerning the use of the social space, Černa louka, situated a short distance from the centre of Ostrava. This project adds new facilities - educational, sports, gastronomical, and scientific - to the original competion proposal. What is more, this brings new input to the area responding to the needs of local inhabitants. The complex of educational buildings is designed as a new principle of vertical compact form.
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Connecting the TV studios
Flimel, Peter ; Křepelka, Václav (referee) ; Filka, Miloslav (advisor)
In this bachelor thesis work is done TV link study in 2020, for the purpose of mediation football matches between studies in Prague and Bratislava. This work deals with optical connections and technology matters to revel in the transmission of large amounts of data.
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The assesment of chloramphenicol residues in biological material, water and feed by GC/MS
Lukačková, Dagmar ; Šalplachta, Jan (referee) ; Rejtharová, Martina (advisor)
This diploma thesis addresses the presence and determination of chloramphenicol residues in biological materials. The theoretical part presents the literature retrieval containing information about veterinary medicaments with the banned use in food producing animals and also the sum of the legislative requirements concerning the presence of these substances in foodproducts and raw food materials of animal origin. The comparison was carried out between the existing analytical methods used for the determination of chloramphenicol residues in different biological materials, which are altogether based on the solid phase extraction for the extract cleaning and the new procedures for sample preparations using columns where the sorbent performs on the molecularly imprinted polymers principle.
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