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Applications of monolithic columns in capillary liquid chromatography
Holdšvendová, Petra ; Coufal, Pavel (advisor) ; Pacáková, Věra (referee) ; Cvačka, Josef (referee)
INTRODUCTION From the nineties of the twent5r's century [l], when appeared the first references about the new type ofthe stationary phases designated for HPLC called monoliths, these became to be the object of interest of several scientific groups [2-4]. Their uncontested advantage in comparison with classical columns is their high stability in pH range from 2 to 12 where there is no possibility to use the classical columns. other advantages are their easy and cheap preparation and the absence of frits on the ends of the separating medium because the monolith is covalently bound to the inner side of the capillary [5]. On the other hand, their disadvantage remains to be worse in reproducibility of the stationary phase preparation than in case of classical columns. This PhD Thesis is focused on monoliths based on organic polymers, mainly the methacrylate monoliths. For the first time, this ýpe of monoliths was shown by Peters et al. [6] and since then there have been made several monolithic phases based on different separating features 17_101. Methacrylate monoliths are convenient for separation of the low molecular substances L7l as well as the high molecular substances like the proteins, oligonucleotides or saccharides n0-121.
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Analysis of cloud storage market
Coufal, Pavel ; Matuštík, Ondřej (advisor) ; Vencovský, Filip (referee)
The first part of the bachelor´s thesis is about cloud computing. It describes general principles, characteristics, models, strengths and weaknesses. The second part focuses on cloud storage services. The main objective of thesis is the cloud storage market´s analysis carried out in the form of comparison between chosen cloud storage providers, based on multi-criterial decision making. The comparison is based on appropriately categorized criteria. Objective of theoretical part is to define cloud computing and its division, describe weaknesses, strengths and pricing models. The contribution of this work is analysis processing, which can serve as a guideline for what to choose or as a model for creating other comparisons.
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