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Study of effect of flavonoids on biotransformation enzymes expression
Koucká, Kamila ; Moserová, Michaela (advisor) ; Mrízová, Iveta (referee)
Flavonoids belong to the group of phenolic compounds included in the secondary metabolites of plants, which are represented mainly in fruits and vegetables. These compounds have antioxidant and anti-carcinogenic effects. Some of them can affect biotransformation enzymes, which include the cytochrome P450 and can interfere with the metabolism of xenobiotics. In the present work we studied effect of dihydromyricetine on the gene expression of cytochrome P450 1A1, 1A2 and cytochrome b5 in livers, lungs, kidneys, colon and small intestine of laboratory rats. The small intestine was divided into three parts namely a proximal, middle and distal. At first, isolation of total RNA was made using commercial reagents and isolated RNA was converted to cDNA by reverse transcription. Finally, using polymerase chain reaction in real time (RT-PCR), the relatively gene expression of genes observed in the organs of laboratory rats pretreated dihydromyricetine and control laboratory rats (untreated) to a reference gene β-actin was determined. It was found that dihydromyricetine did not significantly affect the gene expression of studied genes in most organs. However, a significant decrease of gene expression of CYP1A1 and CYP1A2 was observed in the lungs. On the contrary, an increase of gene expression of CYP1A2 was...
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Use of dance therapy in teaching
Hofmanová Pírková, Alena ; Hanková, Zdeňka (advisor) ; Richterová, Magdalena (referee)
In my work I deal with use of dancing therapy in education through an emotional experience during active dance movement activities. I focus my attention primarily on the perception of the body in space and in relation to the group. My effort is to find a potential in connection of the didactics of the dancing education with the social learning. The goal of my dissertation is to design a dancing-educational project for a specific group of children, aimed at the physical and mental development with emphasis on refinement of the relationships within the group. The project is carried out and afterwards evaluated. KEYWORDS: dance, movement, relationship, upbringing, expression, perception, therapy, diagnose, emotion, music
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Theatre as a Window to the World of Mental Disability
Macková, Anna ; Chrz, Vladimír (advisor) ; Onder, Jakub (referee)
The thesis provides a qualitative analysis of an autobiographical play based on journals written by a mentally challenged woman. The analysis is founded on authentic personal experience, the script of the play and a video recording of its performance. The empirical section is preceded by a theoretical introduction focused on mental disability in relation to theatre. The empirical section begins with the question: What can we learn about a mentally disabled person through theatre? The analytical process consists of three levels: a content analysis of the performance, an analysis of the author's perspective and the viewpoint of an implied spectator. The analysis shows that the story contains common autobiographical topics and the author barely reflects her disability. The creative perspective consists of the process of adapting the story into a play. Theatre also plays a therapeutic role and raises awareness. There is an imaginary window on the boundary between the stage and the audience, one that the author opens from within and the viewer from without. During the course of the play, the implied spectator becomes confronted with their prejudice and an accumulation of ambivalent feelings. This process ends in a catharsis and a genuine encounter with the actor's world and her spontaneity. KEY WORDS...
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The use virus derived vectors for the expression of proteins in plants
Kratochvílová, Kateřina ; Moravec, Tomáš (advisor) ; Holá, Dana (referee)
The tobacco mosaic virus (TMV) is one of the most studied virus. Its properties (simplicity, self assembly of viral capsid, the ability to proliferate in a large variety of hosts) offers a lot of applications in various scientific or biological fields. Gene vectors based on the tobacco mosaic virus represent a very good way for expressing proteins in plants. Because the tobacco mosaic virus is well researched and a large number of his properties have already been discovered, it is used for nanotechnologic sectors in this period. Not only for the formation of nanoparticles, but also for example for a potential production of vaccines in plants. In this paper I summarize the known information about the process of self assembly of tobacco mosaic virus and the possibility of using this virus in the emerging fields of biology. Key words Tobacco mosaic virus, self assembly, viral vector, nanotechnology
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Viral vectors based on tobacco mosaic virus for the expression of recombinant proteins in plants.
Kroupa, Daniel ; Moravec, Tomáš (advisor) ; Plchová, Helena (referee)
(eng): Over the past few decades, plant viruses and vectors derived from them have become not only an integral part of the work in laboratories, but also began to be used commercially for the production of vaccines, drugs, enzymes and other proteins / peptides. One of the most commonly used viruses for the expression of heterologous proteins is tobacco mosaic virus. In this work, I would like to focus on plant viruses and vectors derived therefrom. Compare it with other expression systems used in plants and describe how their benefits could be further used and combined with modern methods of cloning. Key words: viral vector, expression, Tobacco mosaic virus, Cowpea mosaic virus, Golden gate cloning
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The expression profile of cathepsin L in developmental stages of Fascioloides magna
Šašková, Romana ; Kašný, Martin (advisor) ; Mareš, Michael (referee)
Our experimental organism Fascioloides magna is a digenetic liver fluke from Fasciolidae family which parasitizes in domestic and free-living ruminants of North America and Central Europe including Czech Republic. In Czech Republic this highly pathogenic worm causes a severe liver damage to cervids and bovids and the prevalence locally reaches up to 95%. The biology of F. magna including e.g. the characteristics of host-parasite molecular interaction and the functions of particular molecules produced by the parasite are not fully understood. According to results of our previous research the excretory-secretory products of F. magna adults contain number of molecules which play the crucial role in host tissue invasion, digestion and evasion of the host immune response. One of the most abundant is cysteine peptidase cathepsin L (FmCL). FmCL is supposed to play various key roles in biological processes of all stages during a life cycle and therefore we can suppose its different expression level in particular life stages. In order to define the expression level of FmCL we performed the pilot study with miracidia and adults where the qPCR method was applied. The results of this experiment revealed much higher expression level of FmCL1 in adults than in miracidia. The attempt to in situ localize the mRNA...
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Industrial landscape. The possibility of theme in art and in art education
Kuchynková, Monika ; Kornatovský, Jiří (advisor) ; Raudenský, Martin (referee)
This diploma thesis deals with industrial landscape in the visual arts in terms of educational, historical and theoretical viewpoints. In the theoretical part, the author deals with the interpretation of various terms that are related to this industrial issue. At the same time she outlines the development of the industrial landscape, exploration of relationships and attitudes, mostly among the younger generation, to these complexes, and deals with this issue from the aspect of art, such as suggestions or industrial materials. Very interesting and more recent phenomenon is the activity called Urbex, whose interpretation, the world and Czech leaders and its development is briefly explained. Concurrently, this activity is supplemented by visual arts, by the theme of industrial buildings, by industrial material, by memories, by feelings and relation to this landscape of the author of the thesis. In the didactic part, which is crucial to this diploma thesis, the author has developed five preparations for units of teaching in art lessons, where she focused both the use of industrial materials and this theme . Her aim was to acquaint students with these issues and possibilities of working with these materials. Four preparations, which were taught in elementary school in Vodickova, Prague 1, were fine...
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Indication of female gender in new names of professions
Kožuriková, Daniela ; Bozděchová, Ivana (advisor) ; Saicová Římalová, Lucie (referee)
The bachelor's thesis Indication of Female Gender in New Names of Professions is divided into two parts. The first one describes the indication of female gender in linguistics theory and presents main suffixes used for the indication of female gender. The second part tracks occurrence of the expressions excerpted from the vocabulary Nová slova v češtině 1 a 2 and the article Pojmenování nových profesí u pozic v oblasti ekonomiky a podnikání (v rusko- českém srovnávacím plánu) by Ljuba Mrověcová (2009). The expressions were searched for in the corpus SYN 2010 and Neomat database. Lists of names, both found and not found in the sources mentioned above follow. The meaning of less known names is explained in the alphabetical vocabulary.
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Freedom of speech and its limits
Smílek, Ondřej ; Suchánek, Radovan (advisor) ; Jirásková, Věra (referee)
This diploma thesis deals with freedom of speech and its limits. Freedom of speech as the fundamental right which is necessary to proper functioning of a democratic state where the law rules. In this regard the freedom of speech has to be protected properly. Protection in Czech legal order is entrusted with the legal regulation of the major legal force, part of constitutional order, "Charter of fundamental Rights and Basic Freedoms". Freedom of speech as we know it today, was expressed after the Second World War as a prevention of similarly devastating conflict in the future. Certain desire for the free dissemination of ideas we can see in a deep histor when firs attemps of gaining freedom of speech was closely connected to religion. In the 19th century, when the freedom of denomination has found its stable place in the statutes of the most European countries, the freedom of speech has become to be undestood in connection to the freedom of the press, that was the only communicatory media. During the 20th century, when the humankind all over the world witnessed the appalling horror of wars the strict protection od human rights became necessary. For this purpose first international organizations were estabilished and in their scope was formulated first indexes of human rights. The formulation of fundamental...
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The function of the language semiosis in the Quentin tarantino's films
Daičová, Andrea ; Šoltys, Otakar (advisor) ; Štoll, Martin (referee)
Diploma thesis "The function of the language semiosis in the Quentin Tarantino's films" deals with interception and description of the uniqueness of linguistic expressions in the films of one of the most important film directors and screenwriters - Quentin Tarantino. Through semiotic analysis it examines significant elements in movies and screenplays and describes the characteristic method of used expressions. It focuses mainly on characteristics signs and symbols as the main tool of semiosis, and their function in the film language. Diploma thesis focuses also on description of used semantic expressions, explanation of their occurrence in the author's pieces, and trying to capture the originality of the artist's expression. The final part examines Tarantino's role in the cinematography and tries to explain the causes of the great media success.
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