Národní úložiště šedé literatury Nalezeno 8 záznamů.  Hledání trvalo 0.01 vteřin. 
A new method for double bond characterization in lipids by ultraviolet photodissociation mass spectrometry
Rumlová, Barbora ; Strmeň, T. ; Cvačka, Josef
Lipids are structurally diverse biomolecules with vital functions in biological systems. Some of these functions are closely associated with the specific location of the carbon-carbon double bond in the lipid acyl chains. The chain length and the number of unsaturated carbon-carbon bonds can be determined using conventional MS/MS-based structural elucidation methods employing low or higher energy collision-induced dissociation. However, these dissociation techniques do not provide more subtle structural details, for example, the position of unsaturated bonds. Another type of activation method – ultraviolet photodissociation can be used for this purpose. In this study, a new method for characterization of double bond location in lipids acyl chain was developed using 193 nm ultraviolet photodissociation implemented on Orbitrap Fusion Lumos mass spectrometer. This approach is based on the derivatization of the double bond with bis-(5-iodo-[2]pyridyl)-disulfide, subsequent specific cleavages provide a unique diagnostic pair bearing information about the double bond position.
Quantum dot luminescent probe for Caspase 3/7 imaging inside cells
Procházková, Markéta ; Klepárník, Karel
Two step synthesis of luminescent probe based on ligand-exchange in the first step and the specific reaction of amino group in the second step was optimized. The luminescence properties of the final product were checked by time course of\nactive recombinant caspase protein reaction under the model conditions in fluorimeter. The novel luminescent probe enables long-time imaging of active caspases in living cells or tissues.
Epitachophoretic preconcentration of proteins
Hrušková, Helena ; Voráčová, Ivona ; Foret, František
In this work, we present a developed method for preconcentration of standards of proteins cytochrome c, myoglobin, and hemoglobin. Firstly, the electrolyte system was selected and optimized to provide low analysis time, narrow shape of the protein zone, and eliminate overheating at the center of the device. In the next step, the developed method was tested on the preconcentration of proteins: cytochrome c, myoglobin, and hemoglobin. After several adjustments myoglobin and hemoglobin were also uccessfully preconcentrated. After evaluation of pilot results, this technique provides up to fiftyfold preconcentration and recovery of over 70 % for each protein. The next plan is to preconcentrate proteins from complex biological matrices such as urine or blood plasma.
Bioluminescence determination of caspase-3/7 in single osteosarcoma cells
Killinger, Michael ; Matalová, Eva ; Klepárník, Karel
The protein heterogeneity at the single-cell level has been recognized to be vital for an understanding of various life processes during animal development. In addition, the knowledge of accurate quantity of relevant proteins at cellular level is essential for appropriate interpretation of diagnostic and therapeutic results. Here, we determined the level of active caspase-3/7 in physiological and pathological (cancer) conditions by our miniaturized instrumentation.
Chemical vapor generation of cadmium: detector comparison
Sagapova, Linda ; Štádlerová, Barbora ; Svoboda, Milan ; Kratzer, Jan ; Musil, Stanislav
A chemical vapor generation system was connected to an in-house assembled atomic fluorescence spectrometer (AFS) equipped with a miniature diffusion flame atomizer in order to assess its potential to Cd determination. Subsequently, analytical performance of three spectrometric detectors was compared (AAS, AFS, ICP-MS) with respect to Cd determination after volatile species generation.
Novel photochemical vapor generation of rhenium for its ultratrace determination
Vyhnanovský, Jaromír ; Hašlová, K. ; Musil, Stanislav
The presentation focused on novel photochemical vapor generation of rhenium. Details on optimization of generation conditions and other important parameters regarding this sensitive methodology, as well as its possible applications were pointed out and discussed.
Comparison of radiation sources for atomic fluorescence spectrometry
Štádlerová, Barbora ; Dědina, Jiří ; Musil, Stanislav
Comparison of a bismuth boost-discharge hollow cathode lamp and and electrodeless discharge lamp employed as an excitation source in an atomic fluorescence spectrometer.
Optimization of photochemical vapor generation of tellurium for analytical atomic spectrometry
Jeníková, Eva ; Musil, Stanislav ; Hraníček, J.
Photochemical vapor generation of tellurium was studied using a miniature diffusion flame atomizer and high-resolution continuum source atomic absorption spectrometry.