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Immunity of deputies and senators
Popelková, Eva ; Kudrna, Jan (advisor) ; Syllová, Jindřiška (referee)
The presented diploma thesis focuses on the parliamentary immunity of Deputies and Senators in the Czech Republic and consists of two not explicitly titled parts. The first one deals with the genesis of the concept of immunity; the historical course describes the origin of the concept that goes back to the 13th century. Immunity might have been described as protection given by an English monarch towards Representatives; it gradually changed in privileged status recognition of a newly emerging institution, nowadays called Parliament. This excursion back into the 800 year history is supposed to help us understand the basic idea that the immunity of deputies or senators serves as an instrument of protection of a legislative body, or rather the Parliament of the Czech Republic. As for the immunity of deputies (senators) in the Czech Republic, relevant chapters deal with the first attempts to create functional constitutions at the end of the 19th century when Bohemia was part of the Austrian empire. Significant space is dedicated to the 1920 Constitutional Act, which may be considered as a crucial document for today's concept of the immunity of deputies and senators in the Czech Republic. Finally, the first part includes also chapters dealing with later constitutions from 1948 and 1960. The second part...
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Effect of early stress on neuroendocrine regulation, behavior and immnunity
Langová, Veronika ; Vodička, Martin (advisor) ; Buchtová, Helena (referee)
Levels of initial hormones of the hypothalamic-pituitary-adrenal (HPA) axis, corticotropin-releasing hormone (CRH) and arginin-vasopressin, can be altered in adulthood with a context of early-life stress. The effect can be stronger after an acute stress. Some authors suggest that higher levels can be caused by hypomethylation of that genes promotors. High level of default hormones often results in high concentration of corticosterone. Higher concentration of corticosterone in blood can also be supported by a lower level of transcortine, which has been observed in adulthood after a prenatal malnutrition. Locally in tisues is the concentration of corticosterone regulated by 2 types of 11β-hydroxysteroid dehydrogenase. Hyperreactivity of HPA axis can cause an anxiety-like behavior. Anxiety-like behavior is regulated by binding CRH to its receptors. Mice with knockouted gene of the first type receptor (CRHR1) shows generally less anxiety-like behavior. Conversely, some author say that binding CRH to its second type receptor (CRHR2) can inhibit an anxiety-like behavior. Prenatally stressed males show increased expression of CRHR1, prenatally stressed females show reduced expression of CRHR2. Early- life stressed animals also shows a depression-like behavior. It can be related to the presence of some pro-...
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Occurrence of HLA-B27 antigen in patients examined for suspected rheumatological diseases
Lipenská, Zuzana ; Řeháček, Vít (advisor) ; Jílek, Petr (referee)
Author: Zuzana Lipenská Title: Occurrence of HLA-B27 antigen in patients examined for suspected rheumatological diseases Bachelor's Thesis Charles University in Prague, Faculty of Pharmacy in Hradec Kralove Field of study: Medical Laboratory Technician Background: The aim of the study of HLA B27 antigen in patients investigated for suspected rheumatological disease in 2015. Methods: Patient samples examined for the presence of antigen HLA-B27 in the HLA laboratory of the Transfusion Department, University Hospital Hradec Kralove are processed using the serological method. First is used the isolation of peripheral blood lymphocytes using a density gradient, thereafter lymphotoxical test with peripheral lymphocytes. Results: In 2015 were examined 1,329 patients. 207 persons had the HLA-B27, 1122 persons did not. Of the total number of examined patients were examined 754 women and 575 men. Positive was found 108 women and 99 men. The highest incidence of HLA-B27 was observed in the group of 20-29 years old, another slight increase was in the group of 10-19 years old and 40-49 years old. The examined persons had mainly a group diagnosis of disease of the musculoskeletal system with the most frequently diagnosed dorsalgia (M54). Conclusions: The association between HLA-B27 antigen and the deseases has...
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School project to theme vaccination to second grade of primary school and secondary school
Jechová, Markéta ; Pavlasová, Lenka (advisor) ; Skýbová, Jana (referee)
The school project to theme vaccination to second grade of primary school and secondary school This thesis deals with the theme vaccination, its history and principles. The process of immunization is typically made by the administration of vaccines, given in certain time intervals that are determined in accordance with the rules of law. Before vaccine administration, vaccine should be thoroughly checked to protect security of patient's health. Ongoing research and advanced development projects help fight against diseases to protect the human organism. One of the themes that plays role in the vaccination is the financing of research and development as well as financing immunizations itself. As vaccination is as important for adults as it is for children, providing all informations to students is very necessary. To reach this point succesfully, the teching project (described more specifically in this work) is one of the possible ways. However, as project itself has its advantages as well as disadvantages, specific attitude of students and teachers is required accordingly. There are three different projects on vaccinations topics described in the thesis. These three projects were realized in three types of schools: secondary grammar school, third class of central medical lyce and eight class of...
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Research of vertebrate-microbiota relationship using germ-free organisms
Kubovčiak, Jan ; Kreisinger, Jakub (advisor) ; Hájková, Petra (referee)
Germ free (axenic) animals are individuals reared under specific conditions preventing their contact with surrounding microorganisms. Some of the features observed in these individuals vary from those observed in naturally colonized counterparts. These differences probably reflect the influence of presence of a complex intestinal microbial population in the intestine, which influences important physiological functions of the host body by various mechanisms. Thus, nature of these differences allows to study relationship of the host, vertebrate in this case and its microbiota, which evolved into a complicated system of interactions providing relatively stable coexistence. Germ free research of this relationship is focused on interactions between microbiota and host's immune system, metabolism, morphology of digestive tract and behavior. This thesis provides summary of research outcomes on previously mentioned topics. Powered by TCPDF (www.tcpdf.org)
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Universal jurisdiction and the principle of aut dedere aut judicare
Kseničová, Anna ; Šturma, Pavel (advisor) ; Balaš, Vladimír (referee)
This thesis deals with the topic of universal jurisdiction and the aut dedere aut judicare principle. It also marginally deals with issues of immunity of the perpetrators of international crimes, in relation of them enjoying leading functions of the state. In the introductory chapter of this thesis is discussed concept of jurisdiction as a basis for understanding following content of the next chapter about universal jurisdiction itself. In the chapter about the historical development the emergence of universal jurisdiction is described, through circumstances accompanying the establishment of the Nuremberg Tribunal to internationally significant legal proceedings with Albert Eichmann. In my work I try to bring yet ambiguously defined concept of universal jurisdiction, to elucidate its content and scope of its application areas. In the second part of this thesis I focus on the principle aut dedere aut judicare i.e. obligations of states to prosecute perpetrators of international crimes, present at their territory, or else extradite them to the State, which calls for providing their prosecution. On the case before the International Court of Justice in The Hague in the matter of extradition of former Chadian president by Senegal to Belgium I demonstrate the practical implications of the aut dedere aut...
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Exosomes in viral infection and cancer
Sekavová, Alžběta ; Španielová, Hana (advisor) ; Hirsch, Ivan (referee)
Exosomes facilitate intercellular communication and transport of cellular cargo. Understanding the mechanisms underlying the cargo sorting to exosomes and the transport itself is crucial for vaccine development and diagnostic research. Exosome-mediated transfer contributes to immune response as well as progression of several diseases, including cancer and viral infections. Research on exosomes and their role in life cycles of tumorigenic viruses links already known mechanisms of viral carcinogenesis to the transport mechanisms of both cellular and viral proteins and nucleic acids. Epstein-Barr virus employs exosomes for transmission of the LMP1 oncoprotein and regulatory RNAs, whereas human immunodeficiency virus exploits cellular exosomal pathway for hijacking its membrane during budding, which helps it evade the immune system. It has been discovered that hepatitis C virus transfers its infectious virions between cells in exosomes. Exosomes containing oncoproteins and viral RNAs are also released from cells infected with other human tumorigenic viruses. However, mechanisms and implications of such events remain to be discovered. Keywords: exosome, cancer, viral infection, tumorigenic viruses, immunity, in- tercellular communication, hepatitis C virus, Epstein-Barr virus, human immuno- deficiency virus
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International Criminal Court and immunity of the head of state
Sádlová, Martina ; Šturma, Pavel (advisor) ; Balaš, Vladimír (referee)
THE INTERNATIONAL CRIMINAL COURT AND THE HEAD OF THE STATE IMMUNITY The thesis deals with the institute of immunities of the head of the state recognized under the international law and with its applicability before the International Criminal Court (hereinafter referred to as "ICC"). The aim of this thesis is to analyze the conflict between two international legal rules which is the obligation to prosecute and punish perpetrators of crimes under the international law, irrespective of the status of the offender, and the exclusion of heads of the state from the criminal jurisdiction because of the immunity that protects its bearer. According to the Rome Statute (hereinafter referred to as "Statute") which established the ICC its contractual party is obliged to surrender any accused person to the Court even if this person enjoys immunity. However another provision of the Statute says that the request for this surrender could be refused by the party if it broke an obligation which this party has against the state whose national is the accused person. The first chapter outlines the formation and the functioning of the Court. Subsequently, the work deals with theoretical terms such as the head of state, the individual responsibility or the immunity. It provides with the types of immunities a person could enjoy...
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Trans-species polymorphism in immune genes at wildlife animals
Těšický, Martin ; Vinkler, Michal (advisor) ; Matiašovic, Ján (referee)
Trans-species polymorphism (TSP) is described as the occurrence of identical or similar alleles in related species, excluding cases where the similarity arose by convergence. In TSP the alleles are inherited from ancestor to the descendant species. Neutral TSP is frequent in closely related newly diverged species and it gradually disappears. Considering evolution of immune system and host- pathogen interaction, balanced long-lasted TSP is much more important. Balanced TSP in immune- related genes is maintained by the mechanism of balancing selection and commonly persists for millions of years. In my thesis I map the contemporary knowledge on TSP in immune-related genes, focusing mainly on wild animals. The chief emphasis is given to evolutionary mechanisms influencing the width and age of TSP, distinguishing TSP from other TSP-like evolutionary patterns and methodical difficulties with the assignment of TSP. Presently, most articles dealing with TSP concentrate only on genotyping the Major histocompatibility loci (MHC I and MHC II), especially in the peptide binding region. Our knowledge concerning other immune genes with putative TSP is, therefore, insufficient. Most importantly, this is true for innate immunity genes. More effort should be given to comparative large-scale well-sampled studies...
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New ferritin nanoparticles for specific targeting of experimental melanoma in mice: in vitro and in vivo tests.
Rajsiglová, Lenka ; Vannucci, Luca Ernesto (advisor) ; Šírová, Milada (referee)
Cancer diseases represent second most frequent cause of death after cardiovascular diseases in Europe. Nowadays used medical treatments like chemotherapy and radiotherapy are nonspecific and cause huge side effects. Various systems to deliver therapy directly inside the tumour microenvironment and reduce side effects are under development. Protein nanoparticles seem to be very promising strategy to achieve that goal. Our group in cooperation with CNR in Rome tested nanoparticles based on heavy chain of human ferritine. These constructs, modified to expose the tumor targeting molecule, were able to be specifically internalised by B16F10 melanoma cells in vitro. They also specifically target and localise at the sites of primary melanoma and lung metastases of different size in mouse in vivo model. These nanoparticles can carry either therapeutic or diagnostic molecules. Thus they represent a suitable candidate for further studies for potential use in clinical praxis as a diagnostic and/or therapeutic agents (theranostics). Powered by TCPDF (www.tcpdf.org)
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