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Sound installation
Vacek, Lukáš ; Frank, Jakub (referee) ; Ambrůz, Jan (advisor)
In my bachelor thesis, I tried to evaluate my experience with working with sound, vibrations and the way they spread through different types of material. Examine the characteristics of the material and its properties through a minimalist form and through a targeted reduction of the vibrations themselves, while observing how they affect human perception. The fundamental concept of the installation is derived from this relationship. I am fascinated by how vibration and sound in space can create a situation that engulfs the viewer. The significance and the symbolism of this correlation, i.e. the ability of sound to create, distort and transform space and the way humans perceive this action is the main concept of my bachelor thesis. The vibrations represent the constant movement, change and uncertainty that are key aspects of this installation, but also of human existence and its perception. The scenographic nature of the artwork illustrates and alters the space around it with its audiovisual features, giving the impression of its own imaginary architecture. This creates a dynamic dialogue between the objects, which mutually vibrate and resonate through connections between the space and the audience.
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Interaction design with regards to the end user
Macura, David ; Walek, Agata (referee) ; Sedláček, Pavel (advisor)
Tato práce je zaměřena na uživatelská rozhraní, postupy spojené s jejich návrhem a vývojem a interakci mezi počítači a uživateli. Jejím cílem je kodifikovat a prozkoumat nejvýznamnější z těchto postupů a jejich vliv na výsledné produkty, dále popsat teoretickou stránku procesu návrhu a přístup designerů a uživatelů k designu a interakci. V části zaměřené na uživatelská rozhraní má práce za cíl prozkoumat a porovnat vybrané přístupy k tvorbě rozhraní a zmínit nejdůležitější vlivy na požadavky týkající se vývoje rozhraní pro aplikace.
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Preparation and application of physical hyaluronan gel
Kovářová, Lenka ; Krouská, Jitka (referee) ; Venerová, Tereza (advisor)
Bachelor's work is concerning by research in physical hyaluronan gel area. Research is focusing on its preparation, especially by interaction of hyaluronan (HyA) solution with opposite charged surfactant in physiological solution (0,15 M NaCl). In the first part of work I found out influence of molecular weight, concentration of original hyaluronan solution and the ratio of binding sites on hyaluronan chain and surfactant on amount of solids in gel, expressed in percent. Finally we can say, that this value is not influenced by molecular weight of HyA neither relation of binding sides. On the other side, concentration of original HyA solution has significant influence on amount of solids in gel. There was also researched process of gel swelling, where was set amount of water (in percent) able to take dried gel back. Analogical conclusion has been reached like amount of solids in gel. In other part were researched influence of hyaluronan molecular weight and the ratio of binding sites on hyaluronan chain and surfactant on formation of excimers and spectrum change by fluorescence spectroscopy. Pyrene, 1,3-bis(pyren-1-yl)propane (P3P) and prodan were used as fluorescence probes. Excimer emission showed at P3P, while there was no growth of intensity at 470 nm for pyrene. It was found that molecular weight does not affect the value of the ratio of excimer and monomer probe P3P, or the ratio of the first and third peaks of pyrene. The ratio of binding sites HyA and surfactant influence only relation of excimer to monomer (P3P probe), while influence to relation 1:3 hasn't been noticed for pyrene. I have compared spectrum of developed gel for prodan probe, relevant solid and swelled gel by return. It also served for drying and swelling process visualisation.
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Phase separation in dilute solutions of polymers and surfactants
Klementová, Tereza ; Krouská, Jitka (referee) ; Mravec, Filip (advisor)
This bachelor's thesis deals with conditions of phase separation in system of polyelectrolyte (sodium hyaluronate) and cationic surfactant (cetyltrimethylammonium bromide). Separated phase was studied by fluorescence measurement with Nile red as a fluorescence probe and there were also specified rheological properties using flow tests and frequency oscillatory tests. Behaviour of separated microgel was also observed in solution of NaCl.
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Study on the current state of the virtual reality
Bukvald, Josef ; Tůma, Zdeněk (referee) ; Kroupa, Jiří (advisor)
The aim of the Bachelor‘s Thesis is study of the current state of virtual reality. The work is of research character and contains basic information about virtual reality. It also deals with an overview of current technologies and equipment, with examples of application in various fields of science and evaluation of their use.
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New Methods of Study of Reactivity and Transport Properties of Biocolloids
Smilek, Jiří ; Weidlich,, Tomáš (referee) ; Pabst, Willi (referee) ; Klučáková, Martina (advisor)
The main aim of doctoral thesis was the study on reactivity, transport and barrier properties of biocolloidal and synthetic polymeric substances by simple diffusion techniques. It was studied mainly the influence of basic physic-chemical parameters (temperature, concentration, pH and modification of material) on the reactivity and barrier ability of chosen compounds. Further substances were chosen as a model compounds: biocolloids (humic acids, alginate, chitosan, hyaluronate) and synthetic polymer (polystyrenesulfonate). Reactivity, barrier and transport properties of chosen substances were studied by interactions with oppositely charged basic organic dyes (methylene blue, rhodamine 6G, amido black 10B respectively) in hydrogels medium based on linear polysaccharide (agarose). The attention was also paid to basic physic-chemical characterisation (infrared spectroscopy, rheology, elemental analysis, thermogravimetry and scanning electron microscopy) of chosen materials and also hydrogels. Key part of the whole doctoral thesis was the optimization of selected diffusion techniques (diffusion cell technique and non-stationary diffusion in cuvettes) designated for the study on reactivity and barrier properties of wide range compounds (optimized method should be used as an universal method for simple and fast determination of reactivity of different compounds at given or changing conditions). The rate of reactivity, transport and barrier properties was determined based on fundamental diffusion parameters such as diffusion coefficients, break-through time so called lag time, interfacial concentration of chosen organic dye, apparent equilibrium constant, tortuosity factor, partition coefficient.
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Study of aggregation in a biopolymer-surfactant system at low surfactant concentration.
Stiborský, Filip ; Vojtová, Lucy (referee) ; Mravec, Filip (advisor)
In this bachelor thesis, the interaction between hyaluronate and surfactant using fluorescent spectroscopy and pyrene as fluorescent probe was studied. A cationic surfactant cetyltrimethylammonium bromide and a native hyaluronate with different molecular size was used. Before a detected critical micelle concentracion, there were no interactions observed in phosphate buffer. However, we could observe electrostatical interactions in the water systém measuring. At low concentration of surfactant (between critical aggregation concentration and critical micelle concentration), the production of gel phase appeared.
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Study of physical gels with hydrophobic domains
Kovářová, Lenka ; Márová, Ivana (referee) ; Mravec, Filip (advisor)
The thesis is focused on physical hyaluronan gels. The object of study is the interaction of hyaluronan (HyA) with oppositely charged surfactants in physiologic solution (0.15 M NaCl), leading to the formation of gel. In the first part of work have been determined the solids´ contents (X) in gels and their supernatants in percentage and their correlation with molecular weight concentration of original HyA solution and the ratio of binding sites on hyaluronan chain and surfactant CTAB. To conclude, decrease in HyA concentration results in higher values of X and vice versa. On the other hand, increase in the value of X with increasing molecular weight of HyA is not so significant. Analogous conclusions have been made for supernatants and the amount of solids in gel. Drying process has been recorded by drying curves. Swelling process has been used for the characterization of gels. The percentage of water that can be absorbed by dried gel, was determined. The results are in agreement with the measurements of solids´ content in gels. In the next part, the correlation between rheological properties of gels and HyA concentration, HyA molecular weight and concentration of CTAB have been studied by the oscillation and flow tests. The samples with the highest molecular weight and concentration have the most viscoelastic character. The flow test confirmed the assumed pseudoplastic behavior of gels. A very interesting trend arose while comparing HyA concentrations and viscosity in stock solutions and gels. Whereas in stock solution viscosity (at low shear rate) is lower with increasing of HyA concentration, the situation was exactly the opposite in gels. The results are in agreement with frequency tests and observed character of gels.
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Stylized 3D rendering
Jenikovský, Daniel ; Kudělka, Vladimír (referee) ; Vaněk, Vojtěch (advisor)
In my work I create a specific technical solution for creating stylized rendering. Rendering mimics the visuals of 90's games, but I add advanced visual effects to their pixel visuals, common to today's titles. It is created for a specific group project - a game, but it is also possible to use it independently of this project and thus implement it (or its parts) into other future projects.
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Biocompatible Amphiphilic Compounds and their Interactions with Polymers
Burdíková, Jana ; Sedlařík, Vladimír (referee) ; Bakoš, Dušan (referee) ; Pekař, Miloslav (advisor)
This work is focused on the study of interaction between hyaluronan and high-biocompatible amphiphilic molecules. Using fluorescent probe method, screening of the interaction of cationic lipid 1,2-dipalmitoyl-3-trimethylammonium-propane (DPTAP), mixture of this cationic lipid with zwitterionic lipid, 1,2-dipalmitoyl-sn-glycero-3-phosphochloline (DPPC), with hyaluronan, both native and hydrophobically modified was carried out. Results showed the self-aggregation of DPPC and DPTAP independently on lipids ratio in the mixture and the interaction of DPTAP and DPPC/DPTAP aggregates with hyaluronan at specific ratio of DPTAP and hyaluronan concentration. Physical properties of formed membranes and the influence of cholesterol were also investigated at different DPPC and DPTAP concentration ratio. Last but not least, the non-ionic surfactant-DPPC systems were studied, namely, the size of the formed aggregates, the thermodynamics of solubilisation and the interaction with native hyaluronan.
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