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Morphological characteristics of alterations in the striatum induced by neurodegenerative process in the brain
Němečková, Ivana ; Mazurová, Yvona (advisor) ; Anděrová, Miroslava (referee) ; Martínek, Jindřich (referee)
("Morphological characteristics of alterations in the striatum induced by neurodegenerative process in the brain") Huntington's disease (HD) is an inherited neurodegenerative disorder. Although the cause of HD, i.e. the production of the mutant form of unstable protein huntingtin (mhtt) which contains 40 and more CAG repeats is known, the effective therapy is not yet available. Therefore, the use of animal models is crucial for the study of the pathogenesis of this fatal disorder. To date, there is no suitable experimental model simulating the neurodegenerative process (NDP) developing in the striatum of the human HD brain. Most of rodent models of HD fall into two broad categories - the neurotoxic lesions and genetically engineered models. The primary aim of our study was a comprehensive morphological description of the development of NDP of HD phenotype in the striatum of the rat brain. We compared the progression of NDP in the lesion induced by intrastriatal injection of quinolinic acid (QA) and in rats transgenic for HD. The groups of male rats surviving for 3, 6-7, 14 days, 1, 3, 6, 9 and 12 months after the QA lesion were compared with 2-, 6-, 12-, 18-, 22-24-month-old tgHD rats and age-matched control (intact) counterparts in both groups. The primary morphological feature of the NDP of HD...
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The study of binder systems for technology Warm box
Lenghardová, Romana ; Malec, Silvestr (referee) ; Rusín, Karel (advisor)
The project elaborated in frame of engineering studies branch M2332-00. The project is submitting the study of binder systems for technology Warm box. Pursuant to the literary pursuit a problem of the technology Warm box these binders was tested: binder systems based of the Na-silicates, biogenous binders – proteins, polysacharides and fenol resins. Fenol resins and biogenous binders (K2 and Hide glue) showed to be optimal for core making technology Warm box. Biogenous binders are ecologically unexceptionable.
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Programming language and IDE for Lego Mindstorms NXT
Pelc, Jan ; Bureš, Tomáš (referee) ; Štanclová, Jana (advisor)
Title: Programming language and IDE for Lego Mindstorms NXT Author: Jan Pelc Department: Department of Software Engineering Supervisor: RNDr. Jana Štanclová, Ph.D. Supervisor's e-mail address: Jana.Stanclova@ruk.cuni.cz Abstract: The work is focused on design and implementation of a classical programming language for the standard firmware of robotics platform Lego Mindstorms NXT, with respect to less experienced programmers. The work contains a brief description of the target platform and the communication interface between the controller unit and a PC, and an overview of available tools for creating programs for this platform. In the last part, our library for communication with the controller unit is described, and possibilities for remote debugging of programs running on this unit are analysed. Keywords: robotics, Lego Mindstorms NXT, programming language design, compiler implementation, remote debugging
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Food Traceability in Retail Business
Macourková, Kateřina ; Filipová, Alena (advisor) ; Zeman, Jiří (referee)
The target of this master thesis is to map situation of food traceabilty in European Union and in the Czech Republic, document food traceability systems and its echo testing. International and Word organizations which focus on traceabilty and the system of control cannot be left out of consideration .
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Staff performance and motivation in the organization (and design IT to support them)
Biľ, Tomáš ; Surynek, Alois (advisor) ; Chlapek, Dušan (referee)
Paper summarizes the importance of motivation and performance of employees in the organization. The primary goal of this paper is to propose solutions to increase motivation and thus performance. Meet this target would require the implementation of many activities, from finding present, the IS architecture, through detailed analysis of individual systems, which affect workers. Important too was the definition of where all the motivation and performance in man takes and how it can be measured. In the practical part I have done, according to gained experience, using the LMI survey questionnaire to identify weak areas of performance motivation. These results, I finally offered advice and firstly from a psychological perspective, and secondly in terms of IT and its main information system, the workers who come into contact every day and therefore has a huge impact on them. Contribution of the author of this thesis was to identify and increase motivation, through analysis and recommendations specified by the selected methodologies.
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Binary-Code Decoding to a High-Level Representation
Macko, Lukáš ; Soukup, Ondřej (referee) ; Matula, Peter (advisor)
The thesis deals with reverse techniques in software engineering. It presents practical application of software reverse engineering, used tools and approaches. The topic of instruction decoding is discussed in detail. Two basic methods are presented-linear sweep and recursive descent. Their strengths and weaknesses are highlighted. Subsequently a decompiler developed by AVG Technologies is introduced. The decompiler is retargetable. This feature allows to decompile applications of multiple platforms into various target languages. The aim of the thesis is to design and implement algorithm for decoding binary files into high-level representation. The designed algorithm is based on modified recursive descent algorithm, which uses control flow information. In order to achieve more accurate decoding results, symbol table records and other additional information are used. The proposed algorithm was implemented for the AVG Technologies retargetable decompiler. The tests showed that the implemented algorithm improved the function detection in decoded programs. Furthermore, the implemented solution allows to decode files that could not be analysed using the previous version of the decompiler.
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Development of silane modified TS-1 nanoparticles as fillers for Mixed Matrix Membranes (MMM’s)
Martin-Gil, V. ; Lhotka, M. ; Hrabánek, Pavel ; Fíla, V.
In this work, Titanium Silicalite 1 (TS-1) was synthesized using different crystallization temperature (Tc = 180 °C, 160°C, 140°C), aging time (t aging = 1, 2, 3 and 7 days) and aging temperature (T aging = T room , 40 °C, 80°C) in order to get suitable nanoparticle size and good distribution of particle size for using them as a filler in MMMs. The TS-1 particles with different Si/Ti ratios (Si/Ti = 25 and 100) were functionalized using different amino silane coupling agents: 3-aminopropyltriethoxysilane (APTES), 3- aminopropyltrimethoxysilane (APTMS) and 3-aminopropylmethyldiethoxysilane (APMDES). Moreover, a new kind of silane coupling agents: 3- glycidoxypropyltriethoxysilane (GPTES), 3-glycidoxypropyltrimethoxysilane (GPTMS) and 3-glycidoxymethyldiethoxysilane (GPMDES) were also used. Tetrahydrofuran (THF), with a high dielectric constant (7.6), was used as solvent in order to control the surface modification and avoid pore blockage. The samples were characterized using X-ray diffraction (XRD), scanning electron microscopy (SEM), Fourier Transform Infrared spectroscopy (FTIR), as well as carbon dioxide methane and nitrogen adsorption measurements. The obtained results showed that aging temperature at long aging times have the main effect on the morphology of the particles meanwhile crystallization temperature influences gas adsorption properties. T aging higher than T room showed a great influence on the particle size at t aging = 7 days; shorter t aging did not show an observable influence on the particle size. Crystallization temperature influences the adsorption properties; at lower crystallization temperature, higher quantity of CO 2 adsorbed and lower quantity of CH 4 adsorbed. At last, suitable TS-1 filler with good size distribution and particle size about 200nm was obtained using Si/Ti = 100 and Si/Ti = 25, T c = 140 °C, T aging = 40 °C and t aging = 7 days.
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Growth of human adipose-derived stem cells on Ti-6Al-4V alloy with various surface modifications
Kročilová, Nikola ; Bačáková, Lucie ; Pařízek, Martin ; Havlíková, Jana ; Motarjemi, H. ; Molitor, M. ; Gabor, R. ; Marvan, J.
Adipose-derived stem cells (ASCs) are of a great importance for bone tissue engineering. We compared ASCs obtained by liposuction under two pressures (-200 mmHg and -700 mmHg). The number and proliferation activity of cells isolated under -700 mmHg (ASC-H) were higher than in cells isolated under -200 mmHg (ASC-L). However, the ASC-L was more active in osteogenic differentiation, as manifested by a higher intensity of fluorescence of alkaline phosphatase and osteocalcin in these cells. The adhesion and growth of ASCs were then studied on Ti-6Al-4V samples either unmodified (K, roughness parameter R-a = 280 nm) or modified by shot blasting and tarnishing A, R-a = 200 nm), vibratory finishing (B, R-a = 100 nm) and vibratory finishing, shot blasting and polishing (C, R-a = 80 nm). All modified samples were more wettable than the K samples. On day 1 after seeding, the size of cell spreading area on some modified samples was lower than on K samples, namely on B samples in ASC-L, on B and C in ASC-H and on A in control MG-63 cells. On day 3, the cell number on some modified samples became higher than on K samples, namely on C samples (ASC-L), on B (ASC-H) and on B and C s (MG-63 cells). On day 7, the numbers of ASC-H and MG-63 cells on all modified samples evened out and became significantly higher than on K samples. On ASC-L samples, the highest cell numbers were obtained on A samples. Thus, all studied modifications of Ti-6Al-4V enhanced growth of ASCs and human osteoblast-like MG-63 cells.
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Konstrukce elektrického motocyklu
Dobeš, Jan
In diploma thesis Construction of electric motorcycles I deal with issue of electric motorcycles, their types, constructions, advantages and their use. One part includes description of tractions and transmissions. Next part describes batteries, which are necessary in electric vehicles. The aim of this work is to compare different solutions of vehicle constructions. Experiment is about two constructions of quad. This quad used combustion engine and was modified to electric power. Electric quad was tested on power testing stand. Experimental quad constructions which were used were compared and evaluated. Experiment included test of stamina of fully charged battery.
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The effect of housing system on egg shell quality and egg internal quality
Vlčková, Jana ; Tůmová, Eva (advisor) ; Skřivanová, Eva (referee)
In the study 3 experiments were carried out. The objective of the first experiment was to evaluate performance of laying hens, quality of air in poultry house, and microbial contamination of the eggshell in laying hens kept under blue, green, red, and yellow light colour in enriched cages. The laying performance characteristics (hen-day egg production, mortality, and egg weight) were not affected by light colour. Similarly, microbial contamination of the air was not significantly different related to the light colour. There were significant interactions in eggshell contamination between cage floor and light colour in Escherichia coli and Enterococcus. The highest number of Escherichia coli was detected in eggs from hens housed in the middle floor given yellow light and the lowest values on the upper floor also under yellow light colour. Similar results were observed in Enterococcus. The results of our study indicate that the light colour has a minor effect on microbial contamination but the significant influence was in the floor position. The highest microbial egg contamination was found on eggs from the middle floor.
In the second experiment, the effect of housing system (conventional cage, litter, aviary) and feed calcium content (3,0 and 3,5%) on laying performance characteristics, technological quality of eggs, microbial contamination of the eggshell and egg content during storage was evaluated. The significant interaction between housing system and feed calcium content was found in egg weight, eggshell strength, eggshell percentage, eggshell weight, Haugh units and albumen index. The housing system affected egg production, feed intake, egg weight and some characteristics of eggshell quality. From characteristics of eggshell quality the feed calcium content affected only eggshell strength and eggshell percentage. The significant interaction between housing system and storage time was found in contamination of eggshell by total number of microorganisms. The housing system affected also contamination of the eggshell where higher number of Escherichia coli and total number of microorganisms were in eggs from litter. Higher penetration of all monitored species of microorganisms on the eggshell membranes was 2nd and 7th day of storage in the group with 3.5% of calcium in the feed mixture in eggs from the litter. This could be associated with lower quality of eggshell in this type of housing.
In the experiment 3, the effect of different housing system (enriched cage, free range) on technological quality of eggs, microbial contamination of the eggshell, egg content and concentration of protein in albumen during storage was observed. The housing system affected all characteristics of eggshell quality with higher values in cage system. Higher number of pores in eggshell was found in free range. The important relationship between housing system and storage time was indicated in Haugh unit, albumen index, pH albumen and yolk index. The values for characteristics quality of albumen and yolk were better in eggs from cages. Their quality during storage decreased faster in alternative housing systems. There was detected interaction between housing system and storage time also in Escherichia coli, Enterococcus and total number of microorganisms. The highest microbial contamination in fresh eggs was found in free range. The number of microorganisms with storage time significantly decreased faster in eggs from cage system. The penetration of Escherichia coli and total number of microorganisms in albumen was lower in eggs from cage compared to free range. The significant effect of housing system was found in concentration of lysozyme in albumen with higher values in egg from free range. It is obvious that a better quality of the eggs is in the cage housing system. The eggs from this type of housing also remain during storage for longer time in better quality compared with alternative housing systems.
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