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Role of Psb28 proteins in the biogenesis of the Photosystem II complex in the cyanobacterium Synechocystis sp. PCC 6803
BEČKOVÁ, Martina
The thesis focuses on the role of Psb28 proteins, namely the Psb28-1 and its homolog Psb28-2, in the biogenesis of the Photosystem II complex (PSII) in the cyanobacterium Synechocystis PCC 6803. The aims of this work were to localize the proteins within the cells, and to determine their function. A fraction of both Psb28 proteins was identified in the monomeric PSII core complexes but most proteins were found in the unassembled protein fraction associated with thylakoid membranes. Psb28-1 was mostly detected as a dimer while Psb28-2 as a monomer. Psb28-1 also differed from Psb28-2 by its higher affinity to the PSII core complex lacking CP43 antenna. Characterization of Psb28-less mutants suggested regulatory function of the proteins in PSII biogenesis in connection with chlorophyll biosynthetic pathway. Analysis of preparations isolated using FLAG-tagged versions of Psb28 proteins showed their association with Photosystem II - Photosystem I supercomplexes, especially under increased irradiance, and supported a role of Photosystem I in the PSII biogenesis.
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Evaluation of the effectiveness of teaching vocational subjects depending on physical activity before schooling.
Janošíková, Martina ; Hanušová, Marie (advisor)
The period of transition to secondary school brings substantial changes in the requirements for study obligations, autonomy, communication, expression, or separated from their families in the form of staying at the dormitory. Students enrolled in sport centers, in addition to these changes, still struggling with the responsibilities associated with sport. Because they are students first through fourth grades of secondary schools and secondary vocational schools, an age when image self-I am, what I believe in, arises in interactions with the outside world and their own experience, plays a very important role to influence the environment, educators, teachers and trainers, with which students spend most of their time in practice. This study looks at students comprehensively in terms of personal development as a multifactorial biopsychosocial unit, in terms of cognitive and motivational processes, states and properties from the perspective of social psychology dealing with the interactions, relationships and communication, psychology of individuals within the social group and its importance for the development of the individual . It monitors conditions and educational outcomes in terms of educational psychology, dealing with forming properties, opinions, attitudes and value orientation from the perspective of educational psychology.
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Fish passes and their role in landscape and ecosystem management
Míka, Martin ; Horký, Pavel (advisor) ; Kuříková, Pavlína (referee)
Fishways are devices intended to restore fish migration through the transverse obstacles in fragmented flows. Basic types of fish passages can be divided into technical and close to nature. Technical as cellular or crevice between the close to nature can include, for example, a bypass channel or boulder ramps. Close to nature fishways are suitable not only for Migration wider range of species and the size of fish, but they are preferable as landscape elements in urban landscapes. Can be recommended to be close to nature when crossing streams fragmented preferred, although not necessarily their use, for example, due to space constraints at a particular location possible.
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Microbiota of the digestive tract of bees and related insect and the influencing facftors
Hroncová, Zuzana ; Havlík, Jaroslav (advisor) ; Kalous, Lukáš (referee)
This thesis consists of six research chapters, out of which, five have already been published in research journals, 1 is in the process of submission and the last chapter presents original unpublished confidential data in the process of manuscript preparation.
The research presented in this thesis concerns the complex mechanisms of bees and wasps immune system focused on microbiota as component of immunity. As shown in the introduction, highly social and managed species of bees like honey bees and bumble bees, play key roles in natural and agricultural ecosystems worldwide. Recent losses of bees have been attributed to pesticide exposure, poor nutrition, increased parasite loads and habitat degradation. Over the past several years, governments, beekeepers, and the general public worldwide have become concerned by increased losses of honey bee colonies, calling for more research on how to keep colonies healthy. Our main aim was to explore the complex mechanisms of bees and related species gut bacterial populations, their links to insect immunity and investigate the opportunities for an intervention. Part of our aim was to characterise the bumble bee and wasp gut microbiota using 16S RNA sequencing in a search for potentially novel bacterial species.
We have tried to explain how microbiome interacts with the host and showed that major members of these communities appear to benefit the host. The simple gut communities of social bees present ideal model systems to investigate the underlying evolutionary and genetic processes of such interactions. Information based on our results may help in the design of proper probiotic supplementation strategies with respect to physiological conditions in the honey bee gut.
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International Monetary Fund
Drobná, Martina ; Hrdlička, Pavel (advisor) ; Severová, Lucie (referee)
The object of this thesis is an analysis of the current role of the International Monetary Fund in the 21st century. The first part of this thesis is focused on history that led to foundation of this international organisation, the reasons for its establishment and its characterization. The second par introduces one from the most important roles of this Fund, which is providing of credit to the countries that have financial difficulties. The result of this part is an appraisal of these loans and their future development. The last part of this thesis assesses current tasks of the Internationl Monetary Fund and its role in the future.
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The role of authority and form of partnership during creation of new choreographic dance piece
Radačovský, Mário ; KAZÁROVÁ, Helena (advisor) ; WIESNER, Daniel (referee)
In our dance life its very important that we meet time to time people who are so incredibly influencetial to us ether positive or negative ways. Some of them are able to influence us so much that we keep this impact which they have made on us for ever.Role of autority and partnership between these two elements is crucial.Its has impact on everyday of our working life.I ca not imagine what can happen if there is not existing somekind of form of partnership in the dance studio.To be dancer is very no simply just job .Is more ,its something truly special what not everyone can understand.The task of my work is to go back in my profesional past ,through out my own memories and profesional experiences to offer some situations when I truly felt presents of authority and partnership .Sometimes only autorithy .I this work Im trying to offer my small stories which I lived thourgh from my prospective and Im tring to analysed them through my own expierience of today.It always about something.We are creating because we want to say things.Journey is very important sometimes more then final outcome.
Now important during this journey is able to see how things are done how we comunicate them and autorities will appear .
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Community care in psychiatry
BÍNOVÁ, Romana
Abstract Diploma thesis deals with the issue of community care in psychiatry and its objective is to describe the importance of a nurse in providing this care. Community psychiatric care is a very wide area of an intermediary patient assistance designed to help the patients in any areas of their lives. Although in the Czech Republic community care has not achieved the appropriate development yet, its benefits for patients are important now and its importance has been growing. In the theoretical part, after a brief introduction to community care issues, history, principles, but also community care connection with nursing care, are mentioned. Subsequently, attention is paid to particular areas of community care that are important for the mentally ill and the role of a nurse in these areas is described. The approach to the mentally ill, the issue of stigmatization and psychiatric care organizations are mentioned. In the theoretical are part, psychiatric illnesses that may occur in community care are also analyzed and it is explained how community care may be beneficial for psychiatric patients. In the practical part, the objective was to find out the level of psychiatric nurses´ awareness of community care and to determine if they see the sense of community care in psychiatry. Making a survey of community care in which nurses can be involved was another objective of the work. The nurses´ responses to the stated hypotheses were statistically analyzed within quantitative research. The purpose of hypotheses stated was to determine whether nurses with the work experience longer than ten years are more likely to believe that psychiatric care is more beneficial for patients than hospitalization., And also, if nurses with an education higher than secondary have better awareness of community services provision. Other hypotheses were focused on finding out whether nurses consider the most common community care services issue in the Czech Republic to be housing promotion and if nurses aged over thirty years are more aware of the nurses´ role importance in community care. None of those hypotheses were confirmed. The hypothesis assuming that nurses obtain more information on community care from the Internet and literature than at workshops was confirmed. Psychiatric nurses working in mental homes in the South Moravian region and the Vysočina region took part in this research. A component of the practical part is also an analysis of community services in these regions. In both the regions 13 civic associations and community centers were mapped.
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Aversion of the consumer/investor to loss
Svorad, Michal ; Chládek, Libor (referee) ; Škapa, Stanislav (advisor)
This Masters thesis analysis attitude and behavior of consumer in a role of investor in financial market to the loss or how to avoid the risk of future losses .In the theoretical part describes the theory needed to understand the other part, here in after referred to as tools and methods that are used to reduce the risk of loss.
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Vektorová segmentace objemových medicínských dat založená na Delaunay triangulaci
Španěl, Michal ; Martišek, Dalibor (referee) ; Sochor, Jiří (referee) ; Kršek, Přemysl (advisor)
Image segmentation plays an important role in medical image analysis. Many segmentation algorithms exist. Most of them produce data which are more or less not suitable for further surface extraction and anatomical modeling of human tissues. In this thesis, a novel segmentation technique based on the 3D Delaunay triangulation is proposed. A modified variational tetrahedral meshing approach is used to adapt a tetrahedral mesh to the underlying CT volumetric data, so that image edges are well approximated in the mesh. In order to classify tetrahedra into regions/tissues whose characteristics are similar, three different clustering schemes are presented. Finally, several methods for improving quality of the mesh and its adaptation to the image structure are also discussed.
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Optimization of network flow monitoring
Žádník, Martin ; Lhotka,, Ladislav (referee) ; Matoušek, Radomil (referee) ; Sekanina, Lukáš (advisor)
The thesis deals with optimization of network flow monitoring. Flow-based network traffic processing, that is, processing packets based on some state information associated to the flows which the packets belong to, is a key enabler for a variety of network services and applications. The number of simultaneous flows increases with the growing number of new services and applications. It has become a challenge to keep a state per each flow in a network device processing high speed traffic. A flow table, a structure with flow states, must be stored in a memory hierarchy. The memory closest to the processing is known as a flow cache. Flow cache management plays an important role in terms of its effective utilization, which affects the performance of the whole system. This thesis focuses on an automated design of cache replacement policy optimized to a deployment on particular networks. A genetic algorithm is proposed to automate this process. The genetic algorithm generates and evaluates evolved replacement policies by a simulation on obtained traffic traces. The proposed algorithm is evaluated by designing replacement policies for two variations of the cache management problem. The first variation is an evolution of the replacement policy with an overall low number of state evictions from the flow cache. The second variation represents an evolution of the replacement policy with a low number of evictions belonging to large flows only. Optimized replacement policies for both variations are found while experimenting with various encoding of the replacement policy and genetic operators. The newly evolved replacement policies achieve better results than other tested policies. The evolved replacement policy lowers the overall amount of evictions by ten percent in comparison with the best compared policy. The evolved replacement policy focusing on large flows lowers the amount of their evictions two times. Moreover, no eviction occurs for most of the large flows (over 90%). The evolved replacement policy offers better resilience against flooding the flow cache with large amount of short flows which are typical side effects of scanning or distributed denial of service activities. An extension of the replacement policy is also proposed. The extension complements the replacement policy with an additional information extracted from packet headers. The results show further decrease in the number of evictions when the extension is used.
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