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Assesment of the Environmental Impact in the Design of Production Machines in Terms of Greenhouse Gas Emissions of Selected
Krbalová, Maria ; Pačaiová,, Hana (referee) ; Demeč, Peter (referee) ; Blecha, Petr (advisor)
The presented doctoral thesis is focused on environmental impact assessment of basic engineering materials used in a production machine construction. Ecological profile of the machine itself develops already in the phase of its design. It is not only about the choice of future machine parameters and materials that it is built from, but also about technologies used for its manufacture and operation conditions of the finished machine (consumption of energy and service fluids). The thesis occupies in detail with environmental impact analysis of the production machine design from the viewpoint of material production that mentioned machine consists of. The output from the performed analysis is methodology for evaluating of machine design from the viewpoint of greenhouse gas emissions. Created methodology enables evaluating of machine ecological profile and its possible adjustments even during pre-production stage. In the first part of the thesis the analysis of current legislation in the field of fighting against climate changes, reducing of products energy consumption and increasing of production machines energy efficiency is presented. Also in this part of the thesis description of methods that were used to achieve thesis goals is stated. Furthermore analysis of production machine as a system of structural components that fulfil the certain functions and description of used basic engineering materials are presented. The second part of the thesis is devoted to environmental impact analysis of the production machine design process. There the design process and environmental impact of machine design are described. This is followed by description of production machine life cycle and detailed analysis of undesirable substances emissions emitted during pre-production phases of machine life cycle (i.e. during the raw materials extraction and materials production). From this analysis the particular constituents’ pre-production phases which are sources of undesirable substances emissions (e.g. greenhouse gas emissions) were derived. The thesis also includes analysis of these constituents’ life cycles and description of electric power generation as a basic constituent of any phase of product life cycle. In this part of the thesis calculations of particular fuel type’s amounts that is required to produce 1 MWh of electric power and carbon dioxide amount produced during electric power generation are presented. The third part of the thesis contains created models of manufacturing processes of basic engineering materials and calculations of related emissions of selected greenhouse gases. The practical output from this part of the thesis is methodology that enables environmental impact assessment of machine design from the viewpoint of engineering materials used in its construction.
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Antioxidant, anti-proliferative and immunomodulatory effect of fruits, vegetables and mushrooms in vitro
Doskočil, Ivo ; Havlík, Jaroslav (advisor) ; Orsák, Matyáš (referee)
Recently there has been an increasing interest in discovering of new species of plants and mushrooms which have antioxidant or anti proliferative activity. The interest is caused by the fact that these species have medicinal and food utilization. These properties of plants and mushrooms can be used when dealing with many diseases which may be connected with oxidative stress (inflammatory bowel disease, cardiovascular disease, hypertension, and tumour disease, etc.).
The thesis propounds characteristics of in vitro antioxidant and anti proliferative activity of 19 types of juices and 28 methanol extracts of fruits and vegetables, which are common parts of a diet. In the thesis there are also characteristics of 13 ethanol extracts of edible mushrooms of the order of Polypore (Popyporales). Antioxidant activity was quantifie by 2, 2 diphenyl 1 picrylhydrazyl (DPPH), oxygen radical absorbance capacity (ORAC), and inhibition of nitric oxide (NO) production. Cytotoxicity was measured by MTT (3 (4,5 dimethylthiazol 2 yl) 2,5 diphenyltetrazolium bromide. Immunomodulatory activity was determined by an assay of phagocytic activity of human neutrophil granulocytes. Further a total phenolic content (TPC) and total beta glucan content were investigated; these are considered to be the potentially active constituents participating in mentioned activities.
From the obtained results it is evident that juices (TPC = 1603.2 mg GAE/L; ORAC = 438.5 umol TE/g)
and a bilberry (Vaccinium myrtillus) fruit extract (ORAC 836.6 umol TE/g; DPPH = 404.6 umol TE/g) showed
the highest antioxidant activity of all tested samples of fruits and vegetables. Capsicum (Capsicum L.)
juices (TPC = 642.1 mg GAE/L; ORAC = 127.9 umol TE/g) and a radish (Raphanus sativus L.) extract
(ORAC 724.5 umol TE/g; DPPH = 52.2 umol TE/g) also proved to have the high values of antioxidant activity. From the tested fruits and vegetables the following inhibited to produce a nitric oxide: onion (Allium cepa L) juices (lowered the NO production by 57%), tangerine juices (Citrus reticulata Blanco)(by 52%), broccoli (Brassica oleracea var. botrytis italic) extract (by 21%), and orange (Citrus sinensis Pers.) extract (by 10%). Concerning edible
mushrooms, Lentinus tigrinus (Bull.) Fr. (TPC = 216.2 umol GAE/g of extract), Ganoderma lucidum (Curtis) P. Kras (TPC = 257.9 umol GAE/g of extract), and Royoporus badius (Pers.) A.B. De (TPC = 257.8 umol GAE/g of extract) presented the highest phenolic content. Sparassis crispa (Wulfen) Fr. (117.4 mg/g of extract) had the highest content of beta glucan. Substantial effect on phagocytic activity of granulocytes was noticed in connection with Neolentinus lepideus (Fr.) Redhead & Ginns, Polyporus squamosus (Huds.) Fr., and S. crispa. In connection with the latter of mentioned mushrooms, a moderate inhibitory effect towards HT 29 human colorectal adenocarcinoma cell line
(IC50 = 107 ug/ml of extract) was noticed.
The results indicate that the tested plants and mushrooms can be beneficial when reducing the negative effects of oxidative stress. The oxidative stress has been recognized as a contributing factor to a whole range of diseases and the reduction of the oxidative stress may lead to the decrease in the possibility of the diseases´ progression. The results show the possible favourable effects on the human health. When evaluating the results, the following fact should be taken into consideration; the in vitro tests and screenings are considered to be the first phase of systematic research of the effects and serve for the choice of respondents for further detailed studies.
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Passive sampling of organic pollutants in the environment
Čáslavský, Josef ; Kotlaříková, Pavla
Methods of passive sampling of persistant organic pollutants in the environment are reviewed, as well as the most often used types of passive sampler: devices for solid phase microextraction (SPME), stir bar sorptive extraction (SBSE), polymer-coated glass (POG), polyurethan air sampler (PUF), passive air sampler with sorbent, ceramic dosimeter, and semipermeable membrane devices (SPMDs).
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Sharing Local Information for Faster Scanning-Window Object Detection
Hradiš, Michal ; Kälviäinen, Heikki (referee) ; Matas, Jiří (referee) ; Zemčík, Pavel (advisor)
Cílem této dizertační prace je vylepšit existující detektory objektů pomocí sdílení informace a výpočtů mezi blízkými pozicemi v obraze. Navrhuje dvě metody, které jsou založené na Waldově sekvenčním testu poměrem pravděpodobností a algoritmu WaldBoost. První z nich, Early non-Maxima Suppression , přesunuje rozhodování o potlačení nemaximálních pozic ze závěrečné fáze do fáze vyhodnocování detektoru, čímž zamezuje zbytečným výpočtům detektoru v nemaximálních pozicích. Metoda neighborhood suppression doplňuje existující detektory o schopnost zavrhnout okolní pozice v obraze. Navržené metody je možné aplikovat na širokou škálu detektorů. Vyhodnocení obou metod dokazují jejich výrazně vyšší efektivitu v porovnání s detektory, které vyhodnocují jednotlivé pozice obrazu zvlášť. Dizertace navíc prezentuje výsledky rozsáhlých experimentů, jejichž cílem bylo vyhodnotit vlastnosti běžných obrazových příznaků v několika detekčních úlohách a situacích.
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Utilization of waste material from the glass-sand production
Zach, Jaroslav ; Přikryl, Richard (advisor) ; Vavro, Martin (referee)
The presented diploma thesis is focused on possible use of waste from glass and foundry sands deposits of Provodín. The mineralogical and chemical composition, mechanical and physical properties, the specific surface area and parameters of pore area were studied. X-ray analysis proved the presence of kaolinite, illite, quartz and accessory microclin. Silicate analysis shows dominant presence of SiO2 80.52%, then Al2O3 11.36% and K2O 2.14%. This diploma thesis is focused on a possible use of studied material at three main areas: the potential use as a clay component in a raw material mixture for making of hydraulic lime, the use as a kaolinite absorbent and the potential use as a geotechnical material. The studied material was experimentally mixed with limestone in a ratio of 10; 15; 20 a 25 wt %. This mixture was subsequently burnt in the temperature range from 850 to 1,200řC. New phases were identified by an X-ray diffraction analysis. The results prove the presence of characteristic phases for hydraulic limes (C2S, C3A, C4AF). The formation of new phases depend on the temperature. The most of them are formed at a temperature of 1050řC and higher. For the characterization of a specific surface area and parameters of pore areas there were used simple studied material and thermally modified samples...
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Contacts and communications at the turn of early and middle bronze age. The hilltop fortified site at Bánov near Uherský Brod
Chmela, Tomáš ; Stuchlík, Jan (referee) ; Bláhová, Zuzana (advisor)
The goal of this work is to provide a new focus on the hilltop fortified settlement in Bánov, near Uherský Brod (eastern part of the Czech republic). On the ground of the research in 1948 we present the material culture and confirm the dating of the location typicaly into classical phase of Maďarovce-Věteřov culture. Besides the typological-chronological analysis of ceramics was also characterized pottery technology. The whole work is based on interdisciplinary approach towards material culture. Thanks to this we could focus on archaeological contact studies. We conducted micropetrographical analysis of wide amount of ceramical artefacts. From the provenance research point of view we provided following: determinded the ceramics made Banov settlement (heavy clay ware and fine ware); proved local production of some vessels of "foreign style". Based on the detailed analysis of imported ceramic material, we provided a model hypothesis about the existance of two basic interaction levels, in which was fortified settlement in Banov naturally engaged. We mean the economical contacts (represented by exchange of goods) and social contacts (represented by gift exchange).
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Mechanochemical Preparation of Alumina-Ceria
Jirátová, Květa ; Spojakina, A. ; Tyuliev, G. ; Balabánová, Jana ; Kaluža, Luděk ; Palcheva, R.
Ceria containing catalysts play an essential role in heterogeneous catalytic processes. However, ceria shows poor thermal stability and low specific surface area and therefore, many studies have been done to improve its properties by combination with other oxides. Alumina-ceria is substantial component of the three ways catalysts, due to the ceria ability to function as the buffer of oxygen and to enhance the oxygen storage capacity of the catalysts. Ceria in these catalysts also functions as structural promoting component, increasing alumina stability towards thermal sintering. Promising method of oxides preparation, very interesting and simple but not sufficiently studied yet is a mechanochemical synthesis. Here we report on the synthesis of nano-sized alumina, ceria and ceria-alumina of various compositions by a wet solid phase mechanochemical reaction of hydrous aluminum, and/or cerium nitrate with ammonium bicarbonate after addition of a small amount of water. The aim of this contribution is to study processes being in progress during synthesis of the mixed oxides, interaction between components and their mutual effect on the properties of resulting products. The phase evolution during mechanical milling and the subsequent heat treatment of precursors were studied by X-ray diffraction, DTA/TG, H2-TPR, NH3-TPD, CO2-TPD, N2 adsorption at -195°C, IR, and XPS spectroscopy. Alumina and mixtures of alumina with different quantities of CeO2 (1- 18 wt. %) were synthesized by mechanochemical method from aluminum nitrate, cerium nitrate and ammonia bicarbonate.
Fulltext: content.csg -  PDF
Plný tet: SKMBT_C22015083108560 - PDF
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Biochemical methods as tool for study of reproductive proteins
Postlerová, Pavla ; Zigo, Michal ; Pohlová, Alžběta ; Jonáková, Věra
Study of molecular mechanisms in reproduction is essential for the understanding of this outstanding process. Our lab studies proteins secreted by reproductive organs and sperm using various biochemical methods for a long time. We have expertise in protein extraction from spermatic cells using different approaches, and by kits for proteins from the sperm surface and distinct subcellular compartments. The proteins of reproductive organ fluids are separated by chromatographic methods, such as size exclusion chromatography, high-performance liquid chromatography with reverse phase (RP-HPLC) and affinity chromatography on matrices with various ligands. Proteins are subjected to SDS- or 2D-electrophoresis for their characterization and comparison of various extraction methods, different mammalian species, and sperm in different functional development. Electrophoretically separated proteins may be transferred onto nitrocellulose membrane (Western blot) for antibody detection or binding studies with lectin-labelled ligands (lectins, polysaccharides, zona pellucida glycoproteins). We use immunoprecipitation method with specific antibody for protein determination followed by the MALDI identification. Proteins are localized by immunofluorescent techniques on/in spermatic cells and tissue sections of reproductive organs. Isolation of proteins from reproductive tissues and fluids, and the antibody detection is crucial for the studying of reproductive protein origin.
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