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Tcf4 transcription factor in the intestinal epithelium renewal and pathology
Müllerová, Tereza ; Janečková, Lucie (advisor) ; Vávra, Jiří (referee)
TCF4 (T-Cell Factor 4, sometimes also TCF7L2) is an important effector of the canonical Wnt signalling pathway. The transcription factor is produced in many different isoforms with tissue-specific expressionand diametricallyopposing functions.In the intestine,TCF4 is a key factor in epithelial regenerationandmaintenance of stem cell homeostasis. Its depletion causes loss of the proliferatingcrypt compartment and complete breakdown of the intestinal mucosal architecture. The processes in which it acts are therefore vital and must be strongly regulated by the Wnt signalling pathway. Disturbances in TCF4 expression or alternative splicing often lead to a wide range of complex pathologies such as colon cancer, ileal Crohn's disease and type 2 diabetes mellitus.
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Obesity and sleep apnea syndrome
Havrdová, Kateřina ; Matoulek, Martin (advisor) ; Svačina, Štěpán (referee)
Introduction: Obstructive sleep apnea (OSA) is characterized by repeated, complete or partial, obstructions of upper respiratory tract while asleep with accompanied apneic pauses. Interruption of ventilation and the subsequent waking reactions cause fragmented and low-quality sleep in the long term and the simultaneous decline in oxygen saturation of hemoglobin causes development of intermittent hypoxia, which leads to serious complications in patients with sleep apnea. One of the most important etiopathogenic factors in development of obstructive sleep apnea is obesity, mainly central and android obesity. It is estimated that obese patients with BMI ≥ 40 kg/m2 suffer from OSA in over 40-60 % cases and 70 % of patients with OSA are obese. According to current findings, both the maladies influence each other and form a positive feedback loop, which further complicates the whole situation. Aim: The aim of this thesis is to describe the relationship between obesity and obstructive sleep apnea on a set of patients, who were hospitalized in department D3 of III. Internal clinic of Všeobecná fakultní nemocnice v Praze (General university hospital in Prague) for suspicions for diagnosis of obstructive sleep apnea. The main tasks of the practical part of this thesis were to try to find a correlation...
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Lipidomic analysis of adipose tissue in patients with ischemic heart disease
Tomášová, Petra ; Vecka, Marek (advisor) ; Poledne, Rudolf (referee) ; Neckář, Jan (referee)
The ever-increasing incidence of coronary artery disease (CAD) in Czech Republic and worldwide is one of the most important health problem today. Obesity and type 2 diabetes mellitus (DM2T) are risk factors for the development of CAD. Lipid metabolism is greatly affected by these diseases and adipose tissue, previously considered a mere energy store, also plays an important role in these changes. Today adipose tissue is discussed also as an endocrine organ, which effect whole body metabolism significantly. Lipidomic analysis is an effective tool for monitoring lipid metabolism. Lipidomics combines the advantages of determining a large number of analytes and a small amount of sample and its rapid preparation. In the first part of study, I optimized the HPLC-MS method of untargeted lipidomic analysis in adipose tissue. Then, this method was used to compare subcutaneous and epicardial adipose tissue. Finally, I evaluated the effect of CAD and DM2T on the lipid profiles of subcutaneous and epicardial adipose tissues. Optimization of the pre-analytical phase enabled the analysis of 206 lipid molecules (in total) in both types of adipose tissue. Compared to subcutaneous adipose tissue, epicardial adipose tissue contained a higher proportion of phosphatidylcholines (e.g. PC(32:0), PC(32:1), PC(36:4)) and...
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Apoptosis of pancreatic β-cells induced by endoplasmic reticulum stress and its mechanisms in type 2 diabetes
Glatzová, Daniela ; Němcová, Vlasta (advisor) ; Libusová, Lenka (referee)
Increasing incidence of type 2 diabetes represents one of the principal threats to human health in the 21st century. Strong evidence indicates that the rise in incidence of type 2 diabetes is correlated with increasing levels of obesity and that important factor playing role in the development of this disease is an elevation in circulating glucose and fatty acids. Chronically increased concentration of these nutrients was shown to induce apoptosis of pancreatic beta-cells that subsequently contributes to diabetes progression. Despite intensive research, molecular mechanisms underlying this beta-cells loss are still unclear. However, there is increasing evidence that one of the key processes involved in glucose and fatty acid-induced beta-cell death is induction of endoplasmic reticulum stress. The aim of this work is to summarize the recent knowledge about induction of apoptosis by endoplasmic reticulum stress in pancreatic beta-cells in relation to type 2 diabetes.
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Development of mortality on diabetes mellitus in developed countries, focusing on the Czech Republic
Koňařík, Martin ; Burcin, Boris (advisor) ; Hulíková Tesárková, Klára (referee)
Development of mortality on diabetes mellitus in developed countries, focusing on the Czech Republic Abstract This thesis addresses the development of diabetes mellitus mortality in selected developed countries between 1950 and 2008 and compares this development with the situation in Czechoslovakia, the Czech Republic respectively. This development is analyzed by sex, age and type of diabetes. Furthermore it also deals with diabetes morbidity and the share of diabetes mortality from the total mortality. The main approach used in this thesis is analysis of standardized mortality rates and this analysis was done both for whole long-term time series and time series within each International Classification of Diseases for individual countries. Based on the results, it was found that the development of diabetes mellitus mortality was similar across selected countries. It could be divided into several phases. Differentiation between mortality rates of males and females that occurred during reference period was so significant that mortality rates of females which were always higher had dropped below the rates of males. This effect is called in this thesis as a transition from higher female mortality to higher male mortality and it occurred in different countries at different times and also had variable-length...
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New regulatory metabolic factors in patients with obesity and type 2 diabetes mellitus
Matějková, Mirka ; Haluzík, Martin (advisor) ; Flachs, Pavel (referee)
Fibroblast growth factors are proteins with diverse biological function in development, tissue repair, and metabolism. The human FGF gene family consists of 22 members. FGF 19 subfamily includes FGF 19, FGF 21, and FGF 23. They act as systemic factors in an endocrine manner. FGF 19 subfamily requires klotho protein as a cofactor for its action. FGF 19 produced by intestine acts mainly in the liver through FGFR4, where it inhibits bile acid and fatty acid synthesis. FGF 21 is produced by the liver and contributes to the regulation of carbohydrate and lipid metabolism through modulation of glucose uptake in adipocytes. Serum FGF 21 levels are increased in patients with obesity and type 2 diabetes mellitus. Serum FGF 19 levels are on the contrary decreased in patients with obesity and type 2 diabetes mellitus and more probably depend on the nutritional status of the organism than on the glucose metabolism and insulin sensitivity. Key words: type 2 diabetes mellitus, FGF 19, FGF 21, obesity, adipose tissue
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Low carbohydrate diet in type 2 diabetes mellitus therapy
Kábelová, Adéla ; Krejčí, Hana (advisor) ; Šeda, Ondřej (referee)
Type 2 diabetes mellitus (T2D) is a highly prevalent metabolic disorder, which substantially affects quality of patients' lives and simultaneously increases the risk of early death due to development of its specific complications. Current T2D therapy comprises mainly pharmacotherapy, however, lifestyle changes, particularly modification of dietary habits, also assume an important role in the process. While conventional dietotherapy of T2D primarily concerns reduction of dietary fat intake aiming to achieve caloric restriction, the low carbohydrate diet (LCD), recently often discussed in relation to T2D therapy, is based on the reduction of dietary carbohydrates, whereas fats and/or proteins are increased. The positive effects of LCD in T2D therapy and its comorbidities are summarized in the theoretical part of this thesis. The aim of the practical part of the bachelor thesis was to asses the effect of LCD on health parameters associated with T2D as well as the subjective sense of respondents' satisfaction with this diet together with their adherence and compliance. The results of the survey showed that LCD led to weight loss, decreased waist circumference, glycaemia and levels of glycated haemoglobin and led, in a number of cases, to medication reduction. The level of satisfaction with LCD in the...
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Recent trends in therapy of diabetes mellitus type II
Boučková, Karolína ; Herink, Josef (advisor) ; Vopršalová, Marie (referee)
The bachelor's thesis is adressed to diabetes mellitus type 2, the lifestyle disease. It characterizes all its kinds, the laboratory diagnostics and complications. The thesis also contains causes of its appereance, risk factors and clinical manifestations. Nowadays the disease is growing enormously, it strikes people of different ages and genders. That is why the selection of therapy is very important. Particular attention is paid to a standart therapy but also to the latest trends within pharmacological and non-pharmacological treatments. Thanks to suitable therapy and upholding the treatment plan patients can live their life to the full. Key words: diabetes mellitus type 2, clinical symptoms, therapy
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Prevention of complications of 2nd type insulin-dependent diabetes mellitus
Fabianová, Monika ; Eislerová, Iva (advisor) ; Kvasnička, Tomáš (referee)
Bachelor thesis is focused at preventing complications in patients with 2nd type insulin-dependent diabetes mellitus. The aim of the final work was to determine complications associated with the basic disease, prevention of complications, patient education and awareness about diabetes and regime measures. Research was conducted in outpatient clinics for diabetics and subjects of research were 100 adult patients with 2nd type insulin-dependent diabetes mellitus. Research conducted on the basis of a questionnaire survey. The theoretical part focused on basic information about diabetes mellitus in particular classification, diagnosis, treatment, complications, prevention and treatment of disease complications. The practical part was focused on patients with diabetes and activity based on the questionnaire the data were analyzed and graphically processed using tables and graphs. The work showed the results of many research deficiencies in patient awareness and proper education. Many patients do not realize the severity of the disease and the consequences of underestimating the preventive measures. Key words: 2nd type diabetes mellitus, diabetic complications, hypoglycaemia, diabetic foot, prevention of complications, treatment of diabetes, risk factors, insulin treatment.
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