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Interaction between surfactants and hyaluronan with different molecular weight.
Vašíčková, Kamila ; Lehocký,, Marián (referee) ; Pekař, Miloslav (advisor)
The behavior of the system consisted by mixture of two different molecular weight hyaluronates and surfactant was investigated. Mixtures were 17 kDa hyaluronate with 1,46 MDa, 73 kDa with 1,46 MDa, 300 kDa with 1,46 MDa, 806 kDa with 1,46 MDa and 1800 kDa with 1,46 MDa. These compounds were always mixed in the weight ratios 70:30, 50:50 and 30:70. As the surfactant cetrimonium bromide and TWEEN 20 were used. Interactions were studied in aqueous solution with different ionic strength. Sudan red was used as hydrophobic dye. In all experimental series with cetrimonium bromide was observed phenomenon of discontinuous separated phases, described as pearls. Samples containing pearls were tested on stability, were dried and rehydrated back, as were also heated in solution. Subsequently, the particle size was measured in the remaining sample after pearls were filtrated. Mixtures of hyaluronate were characterized by measuring the viscosity using rheology microrheology. It was found that these compounds are heterogeneous and each sample point is not the same viscosity.
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Study of hyaluronan-albumin systems using light scattering methods and calorimetry
Sereda, Alena ; Hermannová, Martina (referee) ; Chytil, Martin (advisor)
This thesis, which is a continuation of the previous Bachelor thesis, is dedicated to the study of polyelectrolyte complexes between hyaluronan (HA) and bovine serum albumin (BSA). Interactions between HA and BSA and a formation of complexes were studied by SEC-MALLS method, where a molar mass, a root mean square (rms) radius, a hydronamic radius and an intrinsic viscosity of particles of the system were defined. Furthermore the interactions were studied by calorimetric measurement ITC, where thermodynamic character of complex formation was determined. Additionally, dynamic and electrophoretic light scattering methods (DLS and ELS) were applied, where hydrodynamic radius and a value of the zeta potential were defined. Also the effect of higher temperature on the character and complex formation was examined by SEC-MALLS and ITC measurements. The interactions were confirmed at any of the used media, but with different efficiency. It was also proved that complexes become smaller in their radii and viscosity with growing BSA concentration. Furthermore it was proved, that the high ionic strength hinders surface charges of HA and BSA molecules and minimizes their mutual interactions. At the higher ionic strength the radii of the complexes, their intrinsic viscosity and zeta potential are increased. The higher temperature has only minimal effect on the formation of the complexes.
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The Phenomenology of Touch and Desire
Vojtěšková, Jitka ; Hanzlová, Alžběta (referee) ; Mikyta, Svätopluk (advisor)
Still life arise from interactions related to a particular situation, location and experience. They are composed of objects that are located on a given place, of my own and borrowed or donated by my "partners". Reflect the time memory. They are a physical reminder of the experience. Still Life is manipulated, staged, composed of various artifacts that are symbolic bearer of facts that preceded them, Images may affect the perception of emotions associated with the concept of beauty, transience, decay, evoking a feeling of emptiness, to reflect the time - an irreversible momentum. Photos show a banal, everyday objects, often so obvious and neglected, What remains for us ... What remains ... What goes around ... What is gone ... Similar scenarios acquire different meanings, finding what is already absent,
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Dynamic light scattering and electroanalytical investigation of hyaluronan-amino acids systems
Muchová, Johana ; Kalina, Michal (referee) ; Chytil, Martin (advisor)
The bachelor thesis deals with interactions between low-molecular and high-molecular weight hyaluronic acid (HA) and protonized amphiphilic amino acids Lysine and Arginine. The interactions were observed in the area of low aminoacids concentrations with in the range 0–15 mmoldm-3. The interactions occur between the carboxyl groups of HA and the amino group of the protonated amino acids. Proving these interactions would allow us to physically modify HA and further more, use such a system as a carrier of pharmaceuticals. The resistence towards the ionic strength at the concentration of 0,015 and 0,15 moldm-3 NaCl was investigated. Previous results show that the system with unprotonated amino acids at low concetrations of electrolyte in the solution is rather disturbing. Therefore, the interactions were reinforced by the aminoacids protonation using HCl. The amino acids were protonated not only completely, but also partly because of negative influence of chloride anions. To study the interactions pH-metry, conductance and Dynamic Light Scattering were used.
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Social Management of Intragender Nudity
Tomková, Hana ; Hájek, Martin (advisor) ; Grygar, Jakub (referee)
Social management of intragender nudity Hana Tomková In this thesis, I examine women's behaviour in spaces with limited privacy, such as changing rooms and showers at sports venues or wellness facilities. In the theoretical part of the thesis I try to use the literature to highlight the issue of judging not only female nudity and the issue of increased female veiling in spaces where intragender nudity is expected. In this first, theoretical, part, I deal with the definition of shame and its possible presence in such spaces. I formulate the assumption that this shame, and by extension increased levels of veiling, may be due to the judgement of bodies based on the prevalence of medical and other professional discourses among the lay public. I use Foucaultian theories of judging the obedience and utility of the body to define such judgments in this thesis, where I attempt to point out that in our, modern, age, experts are not the only ones with the power to judge. In this thesis I have used qualitative research, specifically ethnographic and autoethnographic observations, supplemented by interviews. The research showed that rather than a sense of judgement, it is habit, covering up based on learned stereotypes, the desire to maintain personal space and, last but not least, personal responsibility,...
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Interaction of melted nickel alloys with materials of ceramic cores
Feller, Šimon ; Pernica, Vítězslav (referee) ; Záděra, Antonín (advisor)
This study focuses on the interactions between the IN713LC alloy and various oxide ceramics and oxide coatings commonly used for casting applications in the aerospace or medical industries. The investigated ceramics are based on SiO2, ZrO2, ZrSiO4, Al2O3 and Y2O3. An experimental design proposed for the purpose of this study produced samples used to examine the chemical composition of the metal-oxide ceramic interface. This study compares the findings from the samples with assumptions derived from thermodynamic calculations or thermodynamic conclusions stated in scientific articles with similar themes.
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Dynamics of pollination networks in time and space
Matějková, Zuzana ; Hadrava, Jiří (advisor) ; Tropek, Robert (referee)
This bachelor's thesis focuses on changes in pollination networks on different spatio-temporal scales. It covers in detail the essence and magnitude of these changes in communities of pollinators and plants. The thesis outlines the consequences of these changes for these communities and the way it affects the methodological aspects of studying plant-pollinator networks. I present the existing dynamics of Pollination networks on broad geografical scales as well as changes in local communities. I also describe the temporal changes of communities of plants and pollinators, consisting of diurnal and seasonal dynamics, not exluding the changes on even broader temporal scales. It has been shown that plant-pollinator networks are highly dynamic in space and time. These dynamics consist of species and interaction turnover, as well as of changes in the overall diversity of communities of plants and pollinators. Biodiversity dependent characteristics of pollination networks are affected by the described spatio-temporal dynamics, while characteristics that are biodiversity independent stay relativly stable on broader temporal scales.
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ESP Speaking Activities for Students of Information Technology
Pospíšil, Ondřej ; Smutný, Milan (referee) ; Ellederová, Eva (advisor)
Pojem mluvený jazyk se hojně používá pro označení každého hlasově produkovaného jazyka i neverbální řeči. Produkce mluveného jazyka zahrnuje vyhledávání informací z paměti, vytváření plánů artikulačního programu, exekutivní kontrolu a sebekontrolu. Tyto strategie lze spojit s názvy domén dlouhodobé paměti, motorické kontroly a exekutivní kontroly. Produkce mluveného jazyka se logicky dělí na tři základní kroky: rozhodnutí, co vyjádřit, určení způsobu vyjádření a samotné výsledné vyjádření. Ačkoli dosažení cílů rozhovoru, strukturování vyprávění a modulace průběhu dialogu jsou ze své podstaty rozhodující pro pochopení toho, jak lidé mluví, psycholingvistický výzkum jazykové produkce je většinou zaměřen na formulaci jednotlivých výroků. Z tohoto důvodu hraje vyhodnocení mluveného diskurzu ve třídě důležitou roli při vytváření zásadních poznatků o komplexní povaze třídních struktur, interakcí a vztahů. Tato práce uvádí, jakým způsobem se řeč vyvíjí, jaké důležité principy z gramatického a lingvistického hlediska je nutné dodržovat a v poslední řadě se zaměřuje na rozvoj řeči s ohledem na to, že se nejedná o mateřský jazyk. Pro úspěšného mluvčího je nutné řečové aktivity rozvíjet správným způsobem. Jak má vypadat vhodná aktivita pro rozvoj řečové dovednosti mluvení popisuje poslední kapitola teoretické části této bakalářské práce a její praktická část. Všechny aktivity byly navrženy na základě dotazníkového šetření, které bylo uskutečněno mezi 69 studenty Vysokého učení technického v Brně. Aktivity jsou zaměřeny na rozvoj řečové dovednosti mluvení v oboru informačních technologií.
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Interaction baby and dog or furry help to children
ČECHOVÁ, Adéla
The bachelor thesis aims to present canis therapy as a beneficial method of social therapy with the help of a dog. The theoretical part of the thesis focuses on children with specific needs, characteristics of their disabilities and on suitable interactions between the child and the dog. The listed activities are intended directly for the specific disability in the presence and assistance of the dog. The practical part of the work is focused on interviews with canis therapy workers, on information about working with children with specific needs and the dog, and on interviews with kindergarten teachers about the suitability and benefits of the dog in kindergarten. To supplement the research, a survey was used to find out the awareness of canis therapy among the general public. The conclusion of the work lists activities for the interaction of the child with specific needs and the dog.
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