National Repository of Grey Literature 234 records found  1 - 10nextend  jump to record: Search took 0.00 seconds. 
Plasmid constuction for expression of UDP-glucosyltransferase from Haemonchus contortus
Machalová, Barbora ; Matoušková, Petra (advisor) ; Novotná, Eva (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Barbora Wengrynová Supervisor: doc. Ing. Petra Matoušková, Ph.D. Title of diploma thesis: Plasmid construction for expression of UDP-glukosyltransferase from Haemonchus contortus The barber's pole worm (Haemonchus contortus), like other organisms, uses biotransformation enzymes to metabolize exogenous and endogenous substances. Important representatives of these enzymes are also UDP-glycosyltransferases, which catalyze the formation of a covalent bond between hexose activated by uridine diphosphate and the metabolized compound, thus helping to eliminate undesirable substances. Increased attention to these enzymes is due to the involvement of UDP-glycosyltransferases in the development of resistance to anthelmintics, which are used to treat hemonchosis. The study of their properties and functions can significantly help in solving resistance issues. UGTs are divided into several families and subfamilies depending on sequence similarities. This work focuses on three representatives of UGT in the Haemonchus contortus, UGT24C1, UGT365B6 a UGT368B2. The theoretical part of the diploma thesis includes knowledge about the Haemonchus contortus, hemonchosis and its treatment, biotransformation enzymes and...
Constuction of plasmid and probes for detection of P-glycoprotein in Haemonchus contortus
Funioková, Valentýna ; Matoušková, Petra (advisor) ; Novotná, Eva (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Valentýna Funioková Supervisor: Doc. Ing. Petra Matoušková, Ph.D. Title of diploma thesis: Construction of plasmid and probes for detection of P-glycoprotein in Haemonchus contortus Anthelmintic resistence in parasitic nematodes has become an alarming issue worlwide. Haemonchus contortus, a pathogenic nematode of small ruminants, has become resistant to all types of anthelmintic drugs. Important players of the anthelmintic resistence are ABC transporters, most notably P-glycoproteins. In Haemonchus contortus, 10 homologues of Pgp have been identified. Research of individual P-glycoproteins could reveal particular transporters involved in anthelmintic resistence mechanisms. The diploma thesis focuses on P-glycoprotein-9.2, whose constitutive expression is higher in resistant strains of H. contortus than in susceptible strain. The opening chapters of the diploma thesis deals with the topic of parasitic nematode H. contortus, anthelmintic resistence and description of ABC transporters. The main part of this thesis is dedicated to construction of plasmids for in vitro preparation of digoxigenin-labelled RNA probes, and detection of P-glycoprotein-9.2 mRNA by chromogenic RNA in situ...
The Church of St. John of Nepomuk at Štěchovice
Fatková, Markéta ; Novotná, Eva (advisor) ; Bydžovská, Lenka (referee)
The Church of St. John of Nepomuk in Štěchovice There are not many Art Nouveau constructions in Bohemia. One of them is the Church of St. John of Nepomuk in Štěchovice designed by the architect Kamil Hilbert. Although the construction is not built in the pure Art Nouveau style, its individual parts and elements are taken over for example from the Roman or Renaissance style, it remains probably the most popular architectural example of this style. In view of this fact it is interesting that neither the church nor the builder were devoted sufficient attention in the past. There are not many sources and literature available. Experts devoted their attention to Hilbert only in connection with the preservation of monuments the modernisation of which was his merit at the beginning. Hilbert's own creation remained on the edge of the interest, be it creation dating back to the end of the 19th century or the first decades of the 20th century. The aim of my work is to inform the readers about the personality of the architect Kamil Hilbert and to depict his most important construction, the Church of St. John of Nepomuk in Štěchovice. I focused not only on the brief statement of data, but I tried to incorporate the life and creation of Hilbert also into broader connections, to mention the politic as well as...
University of Agriculture in Prague. Architecture of University Buildings in 1960s
Kopecká, Kristýna ; Novotná, Eva (advisor) ; Schmelzová, Radoslava (referee)
The architecture of university buildings in the '60s of the 20th century The agricultural university in Prague The bachelor thesis is aimed at premises of the Agricultural University in Prague-Suchdol. It thoroughly deals with a history of education in the agricultural branch and its anchoring to a university level, places where it was taught in the past and most significant personalities connected to the field. The following chapters are devoted to the contest for building up these extensive premises, which were the largest planned realization of the capital city in those times. The jurors rated overall 24 projects, that were given over and are introduced. These ratings are then presented together with ratings of the contest in a contemporary press and introduction of all the projects. Location of the premises and the victorious project by Jan Čejka are presented subsequently. I dealt there with the architecture, the technical description of the particular buildings and urbanistic solutions of the premises, also the newer realizations are presented. In the conclusion I attend to the original works of art that were preserved in the premises up to this day. Architekcture ČSR '60s agricultural university Jan Čejka
ARTĚL - The Idea, Activities and Noteworthy Figures in the Association
Skopalová, Markéta ; Rakušanová, Marie (advisor) ; Novotná, Eva (referee)
Synopsis The thesis opens up by reviewing the situation in arts and crafts in the wake of the 20th century. Imperfectly managed transition to mass production caused lower quality. As a result, movements for raising quality arose in Europe. These arts and crafts societies are introduced. The British Arts and Crafts Movement, the Werkstätte in Dresden, the German Werkbund, and the Wiener Werkstätte influenced the Czech scene the most. They became models for the first Czech society of this kind, the Prague Artěl. The history of Artěl is discussed, in particular its creation, development, participation in exhibitions, and links to other organisations. In its final part, the thesis introduces Artěl's most important members and their works. Artel, Prague art group, Arts and crafts, Applied art, 1st half of 20th century
Hrádek u Nechanic - Local Architecture and Its Source of Inspiration
Sieglová, Kateřina ; Kuthan, Jiří (advisor) ; Novotná, Eva (referee)
HRÁDEK U NECHANIC - ARCHITECTURE AND ITS INSPIRATORY SOURCES Chateau Hrádek u Nechanic was built by František Arnošt, the count of Harrach (1799-1884) as a new representative residence of the county family and also as a Harrach's earldom administrative centre. The foundation-stone of the carcass was layed in the year 1839 and the ceremonial laying foundation of the chapel took place two years later. The construction was officially completed in the year 1857 but works in interiors were practically proceeding during the whole second half of the 19th century. Subject of this work is architecture of the chateau Hrádek u Nechanic and its inspiratory sources. We talk about first large aristocratic building in the whole Habsburg Imperium that was in such an integral resemblance influenced by the english Gothic Revival phenomenon - an architecture, an interiors culture and a lifestyle. As an author of building plans is despite many obscurities considered Edward Buckton Lamb (1806-1869). It makes from count František Arnošt of Harrach propably the only one developer in the Habsburg Imperium who gave an order of a project for his chateau directly to an english architect. During the construction were made changes and adjustments by the vienesse architect Karl Fischer who took also a control of bulding. At the...
The detection of protein covalent complexes with DNA using fluorescent microscopy
Melicharová, Růžena ; Jirkovská, Anna (advisor) ; Novotná, Eva (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department od Biochemical Sciences Candidate: Růžena Melicharová Supervisor: PharmDr. Anna Jirkovská, Ph.D. Title of thesis: The detection of protein covalent complexes with DNA using fluorescent microscopy Anthracycline antibiotics are present one of the most potent antineoplastic drugs. The mechanism of their action is complex. They are reported to intercalate to DNA, form DNA adducts and interact with topoisomerase II (TopII) as its poisons. Catalytic cycle of TopII is interrupted when anthracyclines stabilize the covalent complex of DNA and TopII and that causes cell damage. However, using of anthracyclines is limited by several adverse effects e. g. myelotoxicity and cardiotoxicity. The mechanism of cardiotoxicity is still unclear but may be associated with poisoning of the TopIIβ isoform. Unlike the TopIIα, TopIIβ is present mostly in quiescent cells as cardiomyocytes. Furthermore, the only clinically approved cardioprotective drug dexrazoxane belongs to TopII catalytic inhibitors. Nevertheless, the details of the dexrazoxane-afforded protection are unclear. This thesis was aimed to optimize the TARDIS (trapped in agarose DNA immunostaining) assay to detect and quantify covalent cleavage complexes, compare different ways for analysis of...

National Repository of Grey Literature : 234 records found   1 - 10nextend  jump to record:
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