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Development of a bioanalytical method for the determination of novel drug candidates derived from thiosemicarbazone in a biological material
Suprunová, Vlasta ; Stariat, Ján (advisor) ; Kučera, Radim (referee)
Nowadays, high-performance liquid chromatography is widely used analytical method for separation and quantification of drugs in biological material due to its high sensitivity and selectivity. HPLC is suitable for quantitative as well as qualitative analysis. 2-benzoylpyridine-4-ethyl-3-thiosemicarbazone (Bp4eT) is a potential drug from the group of thiosemicarbazones, which are currently intensively studied and developed as anticancer agents. Their specific mechanism of action, based on chelation of iron, might overcome resistance to standard chemotherapy. The aim of this study was to develop and assess essential validation parameters of an HPLC-MS/MS method for determination of Bp4eT and its main phase I metabolites in rat plasma and to utilise the method for the analysis of samples from a pilot pharmacokinetic study in rats. The separation of Bp4eT and its metabolites was achieved on chromatographic column Discovery HSC18 (75 x 4.6 mm, 3 µm) protected by the same type of guard column using the mobile phase consisting of ammonium formate and acetonitrile in ratio 40:60 (v/v). The isocratic elution and the flow rate of 0.4 ml/min were utilised. Mass spectrometer was chosen as a detector and the quantification was performed in selected reaction monitoring mode. Plasma samples were treated using the...
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Posterior Dentition of Modern Humans: the Course of Ontogenesis and its Effect on Size and Shape of a Tooth
Uhlík Spěváčková, Petra ; Merglová, Vlasta (advisor) ; Hovořáková, Mária (referee) ; Králík, Miroslav (referee)
Introduction: The morphological variability of human dentition constitutes an important source of information about biological affinity in evolution and in modern human populations, development of an individual or his health. The variability of upper permanent molars is analyzed consistently with current theorethical model of tooth morphogenesis. The variability of molars is studied here through metameric variation and sexual dimorphism. Most of the morphological variates is placed on the occlusal area of the tooth crown, what makes this area very complex. It is difficult to find suitaible method of analysis for this complexity. We expect geometric morphometrics and dental topogtaphy to deal with this complexity. Material and Methods: We have analyzed dental casts of 160 individuals (F = 74, M = 86), of which we have studied 607 teeth. The morphology of teeth was analyzed in virtual space with the procedures of geometric morphometrics and dental topographical analysis. We have analyzed the variability of occlusal tooth area with landmarks in 2D and 3D space. In the dental topographic analysis we used the RFI and OPCR variables. Results: 2D-GMA and 3D-GMA results were different. 2D-GMA variables were not sexually dimorphic, the only exception was the upper first molar. 3D-GMA variables were all...
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Molecular- genetic analysis of the Thyroid carcinomas
Sýkorová, Vlasta ; Bendlová, Běla (advisor) ; Zamrazil, Václav (referee) ; Peterka, Miroslav (referee)
Introduction: Thyroid cancer represents more than 90% of endocrine tumors and its incidence, predominantly of papillary thyroid carcinoma (PTC), is still increasing in the Czech Republic. Several genetic changes are known, but thein impact to phenotype is still controversial. Aims: To study of the genetic causes (RET/PTC, BRAF and RAS alterations) and the role of RET polymorphisms in thyroid cancer (predominantly PTC), and to correlate genotype with phenotype. Subjects and Methods: Overall 234 PTC tissues, 8 poorly differentiated carcinomas, 3 anaplastic carcinomas, 23 medullary carcinomas, 6 follicular carcinomas and one follicular adenoma were analyzed. Samples of fresh frozen thyroid tissues, fine-needle aspiration biopsies and paraffin-embedded formalin-fixed tissue sections of patients with thyroid cancer and blood samples of healthy controls were used for analysis. The expression of RET/PTC rearrangements was detected on agarose gel. Five RET polymorphisms were analyzed using specific TaqMan probes. Detection of mutations in the BRAF gene and three RAS genes was performed by direct sequencing. Presence of alteration was correlated with clinicopathological parameters. Results: We found out that some RET polymophisms are associated with development of RET/PTC rearrangements in PTC and proved,...
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Analysis of the CRM System of the Chosen Company
Bartošková, Vlasta ; Grus, Vojtěch (referee) ; Klčová, Hana (advisor)
The thesis deals with Customer Relationship Management. The theoretical part focuses on the developement of customer relations and the CRM. Analytical part of the thesis is focused on current state of the CRM system assessment being used. From the obtained information and the revealed weaknesses in this company I seek to introduce a proposal of changes and recommendations that should lead to improve the creation and maintenance of customer relationships.
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Transcendental aspects of architectural design as factors of sustainability
Volnohradský, Radan ; Peřinková, Martina (referee) ; Loutocká, Vlasta (referee) ; Uřídilová, Marcela (referee) ; Urbášková, Hana (advisor)
This thesis deals with complex processes, relationships and phenomena which go beyond the assumed and accepted materialistic perception of the world in general and the process of architectural design specifically. It clarifies and actually redefine the sustainability from a point of higher universal principles forming our environment. At the beginning this thesis presents an extensive knowledge base of existing holistic design systems including Feng-shui, sacred geometry, geomancy or numerology. The purpose is to build and establish a solid foundation for understanding and further research as well as objectively interpreting lesser known topics as a whole. On the basis of intersecting information through the above mentioned topics we specify the hypothesis which proposes the pre-existence of one unifying design matrix of harmonic structures in architecture. The structure of its verification takes us from an analysis of the science of human perception to systems of self-similar contextual references of animated and inanimated forms. These systems of emergent form and flow are basically known as fractals, and could be expressed in both mathematical and geometrical languages. The thesis research then consists of analysing chosen examples of urban and architectural scale in sense of fractality, symbolism and geometrical matrices. We include and integrate the research of associated and relevant phenomena in pedagogical practice, and a case study of the application of fractals in development of a chosen town. From the results of this thesis we abstract five non-dogmatic guidelines or tenets for architectural design; which are supported by experimental verification on some of the author´s buildings. These tenets stand as pillars of implosive architecture. This kind of architecture in context of transcendental overlaps means a possibility of how to bring our anthropogenic environment closer to the natural and harmonic code of the Universe.
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Study of the Ta2O5 insulating layer degradation
Velísek, Martin ; Majzner, Jiří (referee) ; Sedláková, Vlasta (advisor)
The aim of the thesis is to examine the dielectric function Ta2O5 insulating layers in tantalum capacitors. The capacitor plugged in the regular mode represents a MIS structure of reverse direction. Three different factors can be determined for the residual current of the component according to its charge transmission mode: the ohmic, Pool–Frenkel, tunnel and Schottky. An apparatus was constructed by the author of the thesis to measure the temporary connection between residual current and rise of temperature of the tantalum capacitors. Annealing of three different sets of tantalum capacitors made by different producers was performed at the temperature of 400 K and nominal voltage of 35 V during the period of 20 days.The experiment has proved the residual current in the electric field changes with rising temperature in time as a result of the ion movement. The singular factors of the residual current are influenced during the process. By the “ion movement” is meant the ion drift influenced by the attached electric field and diffusion caused by the concentration gradient. First, the samples were being annealed for c. 2 x 106 s, and then the residual current was being regenerated under the voltage of 5 V for 106 s. The residual current values increased considerably after annealing, and decreased again to more or less the original level after the regeneration, some of the samples reaching even values bellow the original level. The VA characteristics of the samples measured before and after the process of controlled obsolescence, and after the regeneration prove not only a change in parameters of the different current factors, but also a change of the current transmission mechanism employed in the process.
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Optical cabling - a comparison
Zelinková, Vlasta ; Křepelka, Václav (referee) ; Filka, Miloslav (advisor)
The thesis is focused on an analysis of mounting and cable laying fiber optic lines. The first chapter includes an analysis is made comparing parameters fiber and copper cabling, mainly in terms of access networks. This part specifies the various transmission media and access networks that are operated on them. Attention is focused on the transmission rate and its possible impact, provided the capacity and other important values. This section also demands site maintenance, service, or any failure on the routes of these networks. With this goal in the second chapter was created several designs networks that provide insight into possible solutions to the situation for emerging networks, and for upgrading existing networks, using both management and elements of optical and copper, or combinations thereof. Provide information from expert sources and verified directs the work of the future and to clarify further the eventual development of individual technologies. Most other space and offers the potential of optical technology, nature, and therefore the third chapter is fully devoted to only the transmission medium. At the beginning of this section is a theoretical analysis of possible ways of cable laying fiber optic cabling with a comparison of advantages and disadvantages. The most stable is an underground installation, which is costly in standard cases. As a result of these factors proved to be a possible solution using the underground installation of water pipeline shutdown. Therefore, the optical path designed, organized and implemented laying of fiber optic cable. By completing the inspection measurement and evaluation of the cost compared with other ways of installing fiber optic cable.
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Self-cleaning Properties of Thin Printed Layers of Titanium Dioxide
Králová, Marcela ; Brezová, Vlasta (referee) ; Krýsa, Josef (referee) ; Veselý, Michal (advisor)
Tenké vrstvy oxidu titaničitého byly imobilizovány na sodnovápenatá skla a skelný uhlík použitím jak techniky materiálového tisku tak metodou chemického napařování. Pro přípravu titaničitých solů byly použity metody sol-gelu a nebo hydrotermální syntézy. Struktura připravených vrstev byla zkoumána rastrovací elektronovou mikroskopií a mikroskopií atomárních sil. Krystalická struktura připravených TiO2 byla analyzovány pomocí XRD metody. Fotoindukováná superhidrofility pripravených vzorků byla charakterizována měřením kontaktních úhlů. Fotokatalytická aktivity připravených vzorků byla testována na fotokatalytické oxidaci 2,6-dichlorindofenolu a kyseliny mravenčí. V případě sol-gel vrstev byl zkoumán vliv množství naneseného TiO2 a množství PEG, který byl do solu přidán jako praskliny potlačující činidlo. V případě hydrotermálních vrstev byl zkoumán vliv času a teploty syntézy a množství vrstev na výslednou účinnost vzorku. U chemicky napařených vzorků byl zjišťován vliv intenzity záření. Byla porovnána fotokatalitická aktivita vrstev připravených materiálovým tiskem a chemickým napařováním a byl vyhodnocen nejaktivnější vzorek.
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Leachability of Cu, Ni and Co from alkali-activated matrices
Janíčková, Vlasta ; Kalina, Lukáš (referee) ; Koplík, Jan (advisor)
The work deals with the determination and comparison of leaching of heavy metals from two types of alkali activated systems based on blast furnace slag and high-temperature fly ash. These systems are capable in their structure encapsulate heavy metals, toxic substances or radioactive substances, which can be harmful for the environment. The aim of this work is to prepare a mixture with acceptable mechanical properties. Leachibility from matrix was tested according to ČSN EN 12457–4, followed by analyses Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES). Structure of matrix was analysed using Scanning electron microscope (SEM).
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