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The Study of the Molecular Structure of Various Forms of Conducting Polymers using FTIR and Raman Spectroscopies
Morávková, Zuzana ; Trchová, Miroslava (advisor) ; Baumruk, Vladimír (referee) ; Matějka, Pavel (referee)
In this Thesis, the structure of thin films formed by a conducting polymer, polyaniline, was studied using mainly infrared and Raman spectroscopies. That led to the study of aniline oligomers. The oligomers play a key role in the formation of thin films and nanostructures of polyaniline. Furthermore, the Thesis deals with the carbonization of various forms of polyaniline (granular polyaniline base, thin films of polyaniline salt, multi-wall carbon nanotubes coated with polyaniline salt or base, polyaniline nanotubes/nanorods prepared in the presence of ethanol). The two topics, aniline oligomers and carbonization of polyaniline, are connected by a paper concerning the carbonization of microspheres formed during oxidation of aniline in alkaline medium. Optical microscopy, transmission and scanning electron microscopy, UV-Vis spectroscopy, spectroscopic ellipsometry, wide angle X-ray scattering and thermogravimetric analysis were used.
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Modification of Physico-Chemical Properties of Biologically Active Compounds
Oktábec, Zbyněk ; Vinšová, Jarmila (advisor) ; Matějka, Pavel (referee) ; Kratochvíl, Bohumil (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Inorganic and Organic Chemistry Candidate PharmDr. Zbyněk Oktábec Supervisor prof. RNDr. Jarmila Vinšová, CSc. Title of Thesis Modification of Physico-Chemical Properties of Biologically Active Compounds The majority of newly developed biologically active compounds (API) belong to the 2. - 4. BCS class, i.e. groups that have problematic permeability and/or solubility. This work deals with modifications of the physico-chemical properties of model biologically active substances. The first group selected for these modifications were bisphosphonates - ibandronate and risedronate. By using a rational selection of co-crystallisation partners, co-crystals of model APIs were prepared with co-crystallisation partners from the group of monosaccharides and their derivatives. Available solid state analytical methods - NIR, Raman spectroscopy, 31 P CP/MAS NMR - were used for the characterization of the resulting cocrystal(s), and in the case of co-crystals of risedronate salt, 13 C CP/MAS NMR, XRPD and DSC were used. There was only one positive sample among all the prepared samples of co-crystals of ibandronate salt, and that was with the use of phenyl-β-D- galactopyranoside as a co-crystallisation partner. PAMPA experiments were...
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Analysis of the Biblical Trust in the World of Pentateuch
Matějka, Miroslav Pacifik ; Hřebík, Josef (advisor) ; Brož, Jaroslav (referee) ; Chalupa, Petr (referee)
This Study elaborates the topic of Biblical Trust in the World of Pentateuch. This trust is understood as to rely in a purely subjective way on a word or instruction of some person. In case of God it frequently means a conviction that some humanly unrealizable promises are to be fulfilled. Such attitude secures to the trusting person merits before God. Rely on God is often understood in a confrontational way as opposite to the life of unbelievers. Trust requires courage to make leap in the dark. Meanwhile it is always right to trust in God, trust in a human person is in Pentateuch interpreted as fool. But if the respective man is the spokesman of God, the eventual mistrust is seen as an attack against God himself. In case of God's revelation trust becomes duty. If there is any doubt people can ask some sign of authenticity for the presented God's will. But if the will is known, eventual mistrust is interpreted as disobedience and follows a severe punishment. The same trust which belongs to God is required also for his prophet and for the Law proclaimed in the God's name. If somebody relies on himself in sense of exaggerated self-confidence which does not count on God, it is understood as a crime. In the same manner is valuated the trust directed towards the pagan divinities.
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Diagnostics of neurodegenerative diseases by means of Raman spectroscopy
Klener, Jakub ; Kopecký, Vladimír (advisor) ; Matějka, Pavel (referee)
Therapies of neurodegenerative diseases are often very difficult and their success depend on an early diagnose. From that reason we have been developing new diagnostic method for multiple sclerosis and Alzheimer disease by drop coating deposition Raman (DCDR) spectroscopy of cerebrospinal fluid (CSF) in this work. We found out conditions of measurements, where spectra were reproducible and accepted for standard diagnostic practices. We discovered that CSF has fast degradation at a room temperature, which was detectable in spectra after 5 hours, and degradation due to refreezing. DCDR spectra of CSF from individual patients were analyzed by factor and cluster analysis. Multiple sclerosis was manifested by lower intensity of a Raman band at 1080 cm−1 , which is probably connected with more general pathologic state. Spectral changes caused by Alzeheimer disease were more complex and beside changes mentioned above also changes connected with composition and conformation of proteins were identified in regions 1200-1800 cm−1 and 2870-2950 cm−1 . Additionally, we succeeded in distinguishing of young healthy patients from older patients in DCDR spectra. In this work were checked up, that DCDR is good diagnostic method for clinical practices for determining neurodegenerative diseases through the complex...
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Synthesis and study of ceramide analogues
Školová, Barbora ; Vávrová, Kateřina (advisor) ; Matějka, Pavel (referee) ; Šklubalová, Zdeňka (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of inorganic and organic chemistry Candidate: Mgr. Barbora Školová Supervisor: doc. PharmDr. Kateřina Vávrová, Ph.D. Title of doctoral thesis: Synthesis and study of ceramide analogues Ceramides (Cer) together with free fatty acids and cholesterol form intercellular lamellar space of the uppermost skin layer (stratum corneum, SC). This lipid matrix presents the proper skin barrier - it protects organism against outer environment and keeps its homeostasis as well. The composition and arrangement of the intercellular lipid matrix is essential for the skin barrier properties. In spite of this fact, the organization of SC lipids at the molecular level is not fully elucidated. The aim of this work is to contribute to more detailed insight into organization of skin barrier using SC lipid model membranes, in particular to find the structural parameters in ceramide molecules, which play a role in the maintenance of the skin barrier. The SC model membranes present a useful tool to study SC lipids, they mimic physiological or pathological conditions in the skin. The obtained results should be used in diagnosing or therapy of skin diseases characteristic with impaired barrier function, such as atopic dermatitis or psoriasis....
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Emotion Detection from Speech
Popková, Anna ; Fér, Radek (referee) ; Matějka, Pavel (advisor)
This Bachelor Thesis deals with research in the field of emotion recognition mainly from speech and marginally from other modalities (video and physiological data). It closely describes the topology of the systems built specifically for the subject of this work. Moreover, it describes experiments leading to optimized pre-processing, regressor training and post-processing. Data used for these research origins from evaluation AV+EC 2015. Results of fusion systems producing the most precise prediction were sent to this evaluation. The Bottle-Neck features are newly tested and combined favorably with commonly used eGeMAPS features for the recognition of arousal. For valence, two kinds of video features are used. Muli-task system (recognizing both valence and arousal) using Bottle-Neck features produces competitive results and is only 13 % relatively behind the mentioned fusion system. This is especially appealing for applications where only audio is available.
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