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Toll-like receptors in bacterial infections
Svobodová, Barbora ; Nachtigal, Petr (advisor) ; Hrnčíř, Tomáš (referee)
Bc. Barbora Svobodová Toll-like receptors in bacterial infections. Diploma thesis Charles University in Prague, Faculty of Pharmacy in Hradec Králové Medical Laboratory Technician Aim: The aim of this diploma thesis was to determine the expression and the activation of the toll-like receptors (TLRs) 2, 4, 5 and 9 in bacterial infections by reverse transcription by real time polymerase chain reaction (RT-qPCR) and by Western blotting (WB). Another aim was to introduce detection of TLRs in a pig by WB in the Institute of Microbiology of the ASCR, v.v.i. in Nový Hrádek. Methods: 1) Optimization of WB - extraction of proteins, protein quantification, electrophoresis, Western blotting, immunodetection of proteins. 2) Four groups of one-week- old gnotobiotic piglets: germfree as a control (GF), piglets infected with Salmonella enterica serovar Typhimurium strain LT2 (LT2), piglets infected with Escherichia coli O55 (O55) and piglets colonized with Escherichia coli Nissle 1917 (EcN). 3) RT-qPCR - purification of a total RNA, spectrophotometric RNA quantification and estimation of purity, synthesis of cDNA, RT- qPCR, normalization and relativization of real-time PCR data. Results: WB analysis of TLR5 in the piglet ileum and mesenteric lymph nodes was optimized. Antibodies against TLR2, 4 and 9 were tested....
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Use of alternative and augmentative communication systems for people with disturbed communication ability
Svobodová, Barbora ; Durdilová, Lucie (advisor) ; Hájková, Vanda (referee)
The presented bachelor thesis deals with the issue of the use of alternative and augmentative communication in people with impaired communication abilities. The text explained the theoretical basis of the work, including concepts: communication, communication disorder, Rett syndrome - RTT and systems of alternative and augmentative communication - AAC. The issue of RTT is processed to serve as a source of information about this rare disease. The thesis is mainly focused on AAC systems usable for people with RTT. The practical part consists of case study of girl with RTT. It analyzes the current development of the child, including her communication skills. It describes the current status of the level of communication skills, discusses progress of training the AAC system VOKS with this girl and analyzes the impact of the use of VOKS on her communication skills development. Keywords: communication disorder, Rett syndrome, pervasive developmental disorders, augmentative and alternative communication, VOKS
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Recreational and tourist exploitation of the central areas of the Giant Mts NP and Beskids PLA
Svobodová, Božena ; Čihař, Martin (advisor) ; Görner, Tomáš (referee)
My thesis deals with evaluation of visits in central parts of Giant Mountains National Park in the summer month through three years of periodic monitoring and unique evaluation survey of visitors of natural protected area of Beskydy. It compares results of this protected areas each other and with other chosen national parks in North America, Australia and Europe. It has been recorded the quantitative load of selected localities in Giant Mountain National Park (Luční bouda, Labská bouda and U čtyř pánů) and natural protected area Beskydy (Pustevny). It were conducted guided interview with visitors at this locations. With this method used was obteined high ratio response (about 92 %). It was recorded a total of 44 281 visitors. The most frequented location was Luční bouda and U čtyř pánů in Giant Mountain National Park. But on the top of the Pustevny, the amount of walkers was twice times bigger. Statistically, it was subsequently found some answers to the dependence of demografic characteristic of population of respondents. From this conclusions were drawn both valid conclusions especially for the population of visitors, compared to other protected areas were also expressed about the conclusions of the participants travel mountain protected areas at all. These conclusions of the thesis will be...
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Sunitinib effects on endothelial expression of hemeoxygenase-I in normotensive rats
Svobodová, Barbora ; Nachtigal, Petr (advisor) ; Němečková, Ivana (referee)
Barbora Svobodová Sunitinib effects on endothelial expression of hemeoxygenase-I in normotensive rats. Bachelor thesis Charles University in Prague, Faculty of Pharmacy in Hradec Králové Medical Laboratory Technician Background: The aim of this bachelor thesis was to define and describe the expression of hemeoxygenase-1(HO-1) in the aortas of normotensive Wistar Kyoto (WKY) rats and evaluate possible effects of sunitinib treatment by means of immunohistochemical and stereological analysis. Methods: Two groups of WKY rats were used for the experiment (6 animals in each of them). Sunitinib was administered to the first group. The other one (the control one) received water only. Application of sunitinib proceeded by following scheme: 8 weeks of using/ 5 weeks rest period/ 2 weeks of using. After that we performed the immunohistochemical analysis on 60 preparations of rat's aortas which observed the expression of HO-1 by methodology En Vision with DAB detection. Then the stereological evaluation was performed. Results: The immunohistochemical analysis showed the expression of HO-1 in the endothelium only and it was noticed just in several animals especially from the group with sunitinib treatment. Conclusion: The immunohistochemical and stereological analysis demonstrated a significant increase of the...
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Effect of gene optimization on recombinant expression of human cytochrome P450 3A4
Svobodová, Barbora ; Martínek, Václav (advisor) ; Bořek Dohalská, Lucie (referee)
Cytochrome P450 3A4 is integral membrane protein residing in endoplasmic reticular membrane. In human the highest concentration cytochrome P450 3A4 is expressed in liver, where it plays a major role in metabolism of many drugs and xenobiotics. The main aim of the thesis was to evaluate the effect of gene optimization on heterologous expression of human cytochrome P450 3A4. At first expression constructs based on vectors pET22b were prepared. Then the efficiency of heterologous expression of optimized vs. natural gene sequence encoding truncated form of the human cytochrome P450 3A4 compared. The results show that the gen sequence optimized for E. coli strains K12 was expressed in significantly higher efficiency than the original human gene based on cDNA sequence. Another aim was to evaluate the suitability of pET22b based expression vectors for recombinant production of native (complete) form of human cytochrome P450 3A4. The amount of native form of the protein found in bacterial membrane was however substantially lower then that of the truncated form. Keywords: cytochrome P450 3A4, heterologous expression, pET22b, gene synthesis
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Impact of English on the Modern Hindi
Svobodová, Blanka ; Vacek, Jaroslav (referee) ; Kostič, Svetislav (advisor)
The aim of this thesis was to provide a description of the linguistic behaviour and repertoire of the speakers of Hindi from the so-called middle-class of the capital city of Delhi, who come into contact with native speakers of English in their employment and everyday life. Therefore, the thesis starts with CHAPTER ONE, where I described and explained the methodology and material used (1.1.0), provided a definition of the middle class in India and its determinants (1.2.0.) in view to my respondents and thirdly gave a detailed insight into the multicultural and multilingual situation in Delhi (1.3.0.), where all of the respondents live. In CHAPTER TWO, I demonstrated the evolution of the language presently known as Hindi, i.e. a language that has been influenced and enriched by various cultures and languages, both from the internal sources such as the Sanskrit based words tatsama (2.1.1.), Hindi based words tadbhava (2.1.2.) and vernacular words called desi (2.2.) as well as external sources, such as Arabic (2.3.1.), Turkish (2.3.2.), Urdu (2.3.3.) or Persian (2.3.4.) and especially the linguistic influences connected with the European Colonial Powers, such as Portuguese (2.4.1.) and above all English (2.4.2.), whose influence started to be felt from the second half of the 19th century and culminated in the...
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The evaluation problems at school
Svobodová, Blanka ; Dvořáková, Markéta (referee) ; Kolář, Zdeněk (advisor)
Tato práce si klade za cíl zmapování problematiky hodnocení ve škole se zaměřením na motivační funkci hodnocení. Teoretická část práce je věnována hodnocení obecně. Zaměřuje se na cíle hodnocení, na formy jejich dosažení, dopad hodnocení na osobnost žáka a utváření sebepojetí, dále na chyby v hodnocení např. nálepkování, naučenou bezmocnost, . . . Zvlášť se práce zaměřuje na analýzu motivační funkce hodnocení. Empirická část práce se zaměřuje na formu převažující motivace a na podílu sebehodnocení žáka v současné škole. Tato část je realizována prostřednictvím dílčí výzkumné sondy. Cílem této sondy je potvrdit či vyvrátit stanovenou hypotézu, která čerpá z teoretické analýzy problematiky školního hodnocení.
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Antimicrobial activity of selected Peruvian medicinal plants
Svobodová, Blanka ; Bosáková, Zuzana (referee)
In frame of this work, antimicrobial activity of ethanol extracts from 14 plants (belonging to 13 families) traditionally used in Peruvian folk medicine for the treatment of various infections was tested against 9 bacteria and 1 yeast by broth microdilution method. For 7 of which this is the first report of antimicrobial activity. Abuta grandifolia, Maytenus macrocarpa, Naucleopsis glabra and Pterocarpus rohrii inhibiting all tested microbial strains at MICs ranging from 0.25 to 8 mg/ml; 0.125 to 0.25 mg/ml; 0.0625 to 4 mg/ml and 0.25 to 16 mg/ml, respectively, were considered as potentially prospective sources of new bioactive substances.
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