National Repository of Grey Literature 178 records found  1 - 10nextend  jump to record: Search took 0.00 seconds. 
The role of human papilloma viruses in ethiopathogenesis malignant tumors in sinonasal area
Sieglová, Kateřina ; Nachtigal, Petr (advisor) ; Petera, Jiří (referee)
The aim of this thesis is the comparison of immunohistochemical and molecular biological methods for the detection of human papillomavirus (HPV) in tumour tissue and their subsequent use in the study of HPV role in the etiopathogenesis of malignant tumors of sinonasal region. Several methods for the purpose of HPV detection were applied: in situ hybridization, immunohistochemical detection of expression of p16INK4a protein, classic polymerase chain reaction combined with two different enzyme immunoassay detection methods, and reverse hybridization, followed by polymerase chain reaction with real time reverse transcription. All methods (p16 DNA in situ hybridization, RNA in situ hybridization, quantitative polymerase chain reaction with reverse transcription - RT-qPCR mRNA) used for the detection of HPV performed very well when compared, showing concordance with statistical significance p ˂ 0,0001. Detection of p16 vs. DNA polymerase chain reaction (PCR) had correlation coefficient p = 0.004 in the whole group, and p = 0.002 in the subgroup of squamous cell carcinoma (SCC). Correlation coefficient between detection of p16 and overall HPV status was 0,659 for entire set and 0,864 for set of SCC, indicating significant linear dependence. Immunohistochemical detection of p16 in combination with the...
Soluble endoglin effects on membrane endoglin signaling in mouse aorta.
Doležálková, Petra ; Nachtigal, Petr (advisor) ; Nejmanová, Iveta (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Author: Petra Doležálková Supervisor: prof. PharmDr. Petr Nachtigal, Ph.D. Diploma Thesis Soluble endoglin effects on membrane endoglin signaling in mouse aorta. Background: The aim of this study was to observe and evaluate the influence of the long- term (12 months) effect of high levels of soluble endoglin on membrane endoglin signalization in the mouse aorta. Specifically, we observed and compared the expression of proteins endoglin, eNOS, and pSmad2/3 in control and a test Sol-Eng+ group of mice. Methods: Two groups of 12-months-old mice with a difference in the level of soluble endoglin were used in this study. Both groups were originated in the same line of transgenic mouse strain CBAxC57BL/6J and fed with ordinary laboratory diet. Western blot technique was used to analyse the expression of individual proteins endoglin, eNOS, and pSmad2/3. The concentration of human soluble endoglin and soluble form of VCAM-1 molecule was evaluated by ELISA analysis. Total cholesterol levels and triglycerides in the plasma were detected by biochemical analysis. Results: Western Blot analysis showed no statistically significant differences in the expression of the membrane endoglin, eNOS enzyme and pSmad2/3...
Effect of glucose treatment on endoglin and biomarkers of endothelial dysfunction in endothelial cells
Adamcová, Adriana ; Nachtigal, Petr (advisor) ; Němečková, Ivana (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Title of Diploma Thesis: Effect of glucose treatment on endoglin and biomarkers of endothelial dysfunction in endothelial cells Author: Adriana Adamcová Supervisor of Diploma Thesis: prof. PharmDr. Petr Nachtigal, Ph.D. Background: The aim of this thesis was to evaluate the expression of endoglin and biomarkers of endothelial dysfunction (eNOS, VCAM-1) in aortic endothelial cells exposed to oscillating glucose. Methods: Human aortic endothelial cells (HAECs) were treated with alternating normal (5 mM) and high (25mM) glucose for 7 days. The control group, was treated with a constant level of normal glucose. Gene expression of endoglin, eNOS, VCAM-1, HIF1α, KLF6, RELA and LXR was measured by quantitative real-time PCR. Flow cytometry was used to detect endoglin and VCAM-1 protein levels. Results: Treatment with high levels of glucose resulted in significantly increased expression of endoglin, eNOS, VCAM-1, HIF1α, RELA and LXR by means of PCR. The flow cytometry method showed a significant difference in protein levels in both endoglin and VCAM-1. Conclusions: The results of this diploma thesis showed that oscillating glucose increases the expression of endoglin and other biomarkers of endothelial...
Membrane endoglin and its role in pathogenesis of endothelial dysfunction in vitro
Vicen, Matej ; Nachtigal, Petr (advisor) ; Mladěnka, Přemysl (referee) ; Štěrba, Martin (referee)
Charles University, Faculty of Pharmacy in Hradec Kralove Department of Biological and Medical Sciences Candidate: M. Sc. Matej Vicen Supervisor: Prof. PharmDr. Petr Nachtigal, Ph.D. Title of Doctoral Thesis: Membrane endoglin and its role in pathogenesis of endothelial dysfunction in vitro Membrane endoglin (Eng) is a transmembrane glycoprotein that acts as a co-receptor in the transforming growth factor β (TGFβ) signalling cascade. Its expression and function have been extensively studied predominantly in relation to endothelial function under various pathological conditions, including hypercholesterolemia and atherogenesis. However, its role in the development of endothelial dysfunction remains controversial. Eng contributes to the formation of nitric oxide (NO) and is crucial in in vivo conditions for the development of the cardiovascular system and especially the heart. On the other hand, it may play an important role in inflammatory adhesion and transmigration of leukocytes to the endothelium, thus contributing to the development of endothelial dysfunction as the first stage of atherogenesis. Experiments on endothelial cells isolated from various blood vessels are used to study short-term effects of substances and drugs on the endothelium. One of the effects studied in endothelial cell...
Lipoprotein-associated Phospholipase A2 in Geriatric Diabetic Patients
Fortunato, Joao ; Blaha, Vladimír (advisor) ; Nachtigal, Petr (referee) ; Soška, Vladimír (referee)
v anglickém jazyce Lipoprotein-associated phospholipase A2 (Lp-PLA2) is an extracellular Ca2+- independent 45 KDa secreted enzyme formed by 441 amino acids. It circulates in plasma in the active form and is encoded in the PLA2G7 gene located on chromosome 6p12-21.1. Lp-PLA2 hydrolyzes several types of short-chain and oxidized phospholipids that harbor acyl groups at the second position of the glycerol backbone. It is mostly bound to low density liproprotein (LDL) particles and it is thought to play a role in the development of cardiovascular diseases (CVDs). Indeed, multiple studies propose that elevated mass concentration and/or activity of Lp-PLA2 is an independent risk factor for the development or recurrence of CVDs. We performed a cross-sectional analysis on 44 geriatric patients aged 79.6 ± 5.6 years that had undergone transcatheter aortic valve implantation (TAVI) or ballon valvuloplasty (BV) for the treatment of severe aortic stenosis. Lp-PLA2 mass concentration was already increased in elderly patients before TAVI or BV and significantly increased after the procedures. We found strong correlations with LDL concentration (LDL-C), total cholesterol and triglycerides. Baseline Lp-PLA2 mass concentration was increased in diabetic patients comparing to non-diabetic patients. We then discuss and...
Effect of soluble endoglin on bile acids metabolism in the liver of high-fat diet-fed transgenic mice.
Jiroušková, Barbora ; Nachtigal, Petr (advisor) ; Schreiberová, Jolana (referee)
Barbora Jiroušková Effect of soluble endoglin on bile acids metabolism in the liver of high-fat diet-fed transgenic mice. Diploma thesis Charles University, Faculty of Pharmacy in Hradci Králové Pharmacy Background: Increased plasma concentrations of soluble endoglin (sEng) have been demonstrated in metabolic disorders such as hypercholesterolemia or insulin resistance. Metabolic diseases and inappropriate diet are the basis for the development of NASH (Non-alcoholic steatohepatitis). The aim of this diploma thesis was to determine how high plasma concentrations of sEng affect the bile acids metabolism in the liver of mice fed with high-fat diet, which induced NASH. Methods: Three-month-old male mice with CBAxC57BL/6J base (n = 8, in each group) were used in experiment: 1) "wild-type", which were fed a standard diet (WTchow) for 6 months; 2) "wild-type" male mice fed a high fat diet (cholesterol, glucose and fructose) (WTHFD); 3) males expressing human sEng (sENGHFD) which were fed a high fat diet, glucose and fructose. In vivo kinetic study was performed to determine bile flow. Expression of transport proteins responsible for bile acids transport was performed at mRNA and protein level by qRT-PCR and Western blot. Results: High levels of soluble endoglin did not significantly affect BA homeostasis...
Soluble endoglin role in the pathogenesis of endothelial dysfunction
Vitverová, Barbora ; Nachtigal, Petr (advisor) ; Vrablík, Michal (referee) ; Mladěnka, Přemysl (referee)
Charles University, Faculty of Pharmacy in Hradec Kralove Department of Biological and Medical Sciences Candidate: Msc. Barbora Vitverova Supervisor: prof. PharmDr. Petr Nachtigal, Ph.D. Title of Doctoral Thesis: Soluble endoglin role in the pathogenesis of endothelial dysfunction The endothelium is a major regulator of vascular homeostasis and exerts a number of vasoprotective effects, including production of vasodilating agents, suppression of smooth muscle cell growth, or inhibition of inflammatory responses. Most of these effects are mediated by nitric oxide (NO), the most effective endogenous vasodilator. Decreased NO production or its activity is a hallmark of endothelial dysfunction leading to an impaired endothelial-dependent vasodilation. Endothelial dysfunction is the first and key step in the development of atherosclerosis and can be detected before structural changes in the vessel wall. Atherosclerosis is a complex inflammatory disease underlying many cardiovascular disorders including ischemic heart disease and represents the major cause of morbidity and mortality in developed countries. Statins are drugs of choice in the treatment of atherosclerosis and ischemic heart disease, which have been shown to reduce LDL cholesterol levels and modulate endothelial function with their...
Morphological alterations in kidneys in mice with high soluble endoglin levels
Vyskočilová, Martina ; Němečková, Ivana (advisor) ; Nachtigal, Petr (referee)
Masters thesis Morphological alterations in kidneys in mice with high soluble endoglin levels Author: Bc. Martina Vyskočilová Study program: Medical bioanalytics Charles University Faculty of Pharmacy in Hradec Králové Background: Endoglin (Eng) is a transmembrane glycoprotein also known as an accesory receptor type III for a TGF-β molecule which is expressed primarily on the surface of endothelial cells. Endoglin plays a role in vascular homeostasis, angiogenesis and cardiovascular development. Soluble endoglin (sEng) which is formed by a cleavage of an extracelullar part of a membrane-bound endoglin scavenges the TGF-β molecules in the serum and blocks their binding to the membrane-bound receptor. Soluble endoglin is considered as a trigger of an endothelial dysfunction which is associated with cardiovascular diseases and patogenesis of preeclampsia. The aim of this study is to describe possible effect of high levels of a soluble endoglin on kidney tissue of transgenic mice in comparison with kidneys of mice with low levels of a human soluble endoglin. Methods: Kidneys of twelve months old mice with high and normal levels of a human soluble endoglin on a standard laboratory diet were fixed in 4% formaldehyde, embedded in paraffin and sliced into 7 μm thin sections. One of these sections was...
Membrane endoglin and its role in pathogenesis of endothelial dysfunction in vitro
Vicen, Matej ; Nachtigal, Petr (advisor) ; Mladěnka, Přemysl (referee) ; Štěrba, Martin (referee)
Charles University, Faculty of Pharmacy in Hradec Kralove Department of Biological and Medical Sciences Candidate: M. Sc. Matej Vicen Supervisor: Prof. PharmDr. Petr Nachtigal, Ph.D. Title of Doctoral Thesis: Membrane endoglin and its role in pathogenesis of endothelial dysfunction in vitro Membrane endoglin (Eng) is a transmembrane glycoprotein that acts as a co-receptor in the transforming growth factor β (TGFβ) signalling cascade. Its expression and function have been extensively studied predominantly in relation to endothelial function under various pathological conditions, including hypercholesterolemia and atherogenesis. However, its role in the development of endothelial dysfunction remains controversial. Eng contributes to the formation of nitric oxide (NO) and is crucial in in vivo conditions for the development of the cardiovascular system and especially the heart. On the other hand, it may play an important role in inflammatory adhesion and transmigration of leukocytes to the endothelium, thus contributing to the development of endothelial dysfunction as the first stage of atherogenesis. Experiments on endothelial cells isolated from various blood vessels are used to study short-term effects of substances and drugs on the endothelium. One of the effects studied in endothelial cell...
Effect of glucose treatment on endoglin and biomarkers of endothelial dysfunction in endothelial cells
Adamcová, Adriana ; Nachtigal, Petr (advisor) ; Němečková, Ivana (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Title of Diploma Thesis: Effect of glucose treatment on endoglin and biomarkers of endothelial dysfunction in endothelial cells Author: Adriana Adamcová Supervisor of Diploma Thesis: prof. PharmDr. Petr Nachtigal, Ph.D. Background: The aim of this thesis was to evaluate the expression of endoglin and biomarkers of endothelial dysfunction (eNOS, VCAM-1) in aortic endothelial cells exposed to oscillating glucose. Methods: Human aortic endothelial cells (HAECs) were treated with alternating normal (5 mM) and high (25mM) glucose for 7 days. The control group, was treated with a constant level of normal glucose. Gene expression of endoglin, eNOS, VCAM-1, HIF1α, KLF6, RELA and LXR was measured by quantitative real-time PCR. Flow cytometry was used to detect endoglin and VCAM-1 protein levels. Results: Treatment with high levels of glucose resulted in significantly increased expression of endoglin, eNOS, VCAM-1, HIF1α, RELA and LXR by means of PCR. The flow cytometry method showed a significant difference in protein levels in both endoglin and VCAM-1. Conclusions: The results of this diploma thesis showed that oscillating glucose increases the expression of endoglin and other biomarkers of endothelial...

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