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Analysis of Denatured PCR Products Modified with 7-deazapurine Bases at Hanging Mercury Drop Electrode
Dudová, Zdenka ; Špaček, Jan ; Havran, Luděk ; Pivoňková, Hana ; Fojta, Miroslav
7-deazapunines are synthetic analogues of natural purine nucleobases which can pair with pyrimidines, retaining pairing complementarity of their parent purines. There is replaced N7 atom by CH group in 7-deazapurines so that DNA modified with them don't participate in Hoogsteen basepairing and don't form alternative structures. Here is a study of 7-deazapurines incorpotated into DNA measured at HMDE by CV and ACV. While 7-deazaadenine was reduced at the HMDE, giving rise to a similar irreversible cathodic peak as the natural adenine, 7-deazaguanine didn't yield any peak analogous to the peak G due to Guanine, in Agreement with a loss of corresponding redox in 7-deazaguanine.
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Novel Reactive Groups for Modification DNA by Oxoosmium Complexes
Havran, Luděk ; Vidláková, Pavlína ; Špaček, Jan ; Hermanová, Monika ; Fojta, Miroslav
DNA is naturally electroactive moleclue producing several intrinsie voltammetric signals. Some of them found appliction in electrochemical analysis of DNA interactions and damage. In particular types of applictaions is a suitable use DNA labelling by redox active tags to increase selectivity and sensitivity of analysis.One type of long-term used tags are complexes of osmium tetroxide with nittrogen ligunds (Os,L). These complexes preferentially react with pyrimidines in single stand DNA producing electractive adducts. primary reaction site for Os,L is C-C double bond in pyrimidine nucleobases. In this contribution will be presented results acquired with DNA containing chemically modified nuclebase.
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Electrochemical Study of Osmium Tetroxide Comptests Reactivity to DNA Bearing Butylacrylate
Havran, Luděk ; Havranová-Vidláková, Pavlína ; Špaček, Jan ; Vítová, Lada ; Hermanová, Monika ; Fojta, Miroslav
Coniplexes of osmium tetroside with nitrogen ligands (Os,L e.g.. with 2,2'-bipyridine (bpy) find application in redox labelling of DNA.. probing of DNA structure, and in studies of DNA interaction with other molecules Os,L preferentially react with pyrimidines in single strand DNA producing electroactive adducts Primary reaction site for Os,L is C=C double bond in pyrimidine nucleobases. In this contribution we introduce a new two-step technique of DNA modification with Os,bpy, consisting in enzymatic construdion or DNA beating butyl acrylate (BA) moieties attached to uracil or T-deaza adenine, followed by chemical modification of a reactive C=C double bond in BA residue.
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Safety inspections in region Vysocina
Špaček, Jan ; Radimský, Michal (referee) ; Matuszková, Radka (advisor)
This thesis presents a safety inspection in selected places in the Vysocina region of the Czech Republic. Six intersections in rural and municipal areas, plus three rural road sections were selected for the inspection and an accident rate analysis. The places selected were compared in terms of the accident rate and one case of each group was then selected for a detailed safety inspection encompassing visual inspection and identification of possible hazards. By eliminating such safety hazards, it is possible to ensure a traffic fluency and to preclude traffic accidents or at least minimize the harm caused by it.
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Bass string instruments used in church and folk music in Prague and central Bohemia
Špaček, Jan ; ŽALUD, Radomír (advisor) ; HUDEC, Jiří (referee)
This thesis is aiming to summarize and compare instruments used in church and folk music in Prague and central Bohemia. At first it names various types
of instruments and describes their characteristics. Then pick few makers
who influenced development and expansion of double bass in the land, and following
with pick of particular instruments and their description.
Last part of this thesis speaks of Ondrej Hornik and his collection, which
is valuable source of information and tangible documentation of musical development in Czechia.
Included is an insert located at the end of this work.
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Integration of the Corona renderer into the ArchiCAD software
Špaček, Jan ; Křivánek, Jaroslav (advisor) ; Vévoda, Petr (referee)
Integration of the Corona renderer into the ArchiCAD software Bachelor thesis - Jan Špaček, 2018 We present a prototype of a plug-in that integrates Corona Renderer into Archi- CAD. We strive to make Corona in ArchiCAD as easy to use as in other graphics applications, so the user interface is a major concern in our plug-in. Special focus was given to interactive rendering, which has proved to be a technical challenge in other host applications. We give a brief overview of the APIs that we use and describe the architecture and implementation of the plug-in. We also present the performance of interactive rendering on various scenes. 1
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Audio D/A converter with external control using microcontroller
Špaček, Jan ; Stifter,, Jiří (referee) ; Kratochvíl, Tomáš (advisor)
This diploma thesis deals with the design of a laboratory measurement tool with ADC converter for audio and low frequency signals. In the introduction part a theoretical background of the ADC and DAC conversions are outlined. In the next chapters the technical solution of the laboratory converter is presented, including DAC with integrated circuit AD1852 and completed with digital audio receiver CS8416. This circuit secures compatibility with external audio devices with digital interfaces, like SPDIF or AES3. The digital receiver and audio DAC are added with serial communication interface for setup of the most audio and input parameters. This setup is operated by external microcontroller ATmega32 and original firmware. The thesis contains block diagram and schematic layout of the laboratory converter, including complete technical documentation for the construction and design.
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Design of urban area using advanced technologies
Špaček, Jan ; Apeltauer, Tomáš (referee) ; Remeš, Josef (advisor)
This thesis focuses on the design of urban areas in regards of the air flow and the creation of Urban Heat Islands. It reviews basic aspects influencing the design of streets. Every building, tree, or obstacle obstructs the air flow and creates the wind shadow, where the air pollution increases. It also creates areas of higher wind speed, in which public comfort is reduced. By understanding the importance of this process and by implementing it into the urban planning process, using advanced technology we can create healthy urban environment.
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