Original title: Electrochemical Study of Osmium Tetroxide Comptests Reactivity to DNA Bearing Butylacrylate
Authors: Havran, Luděk ; Havranová-Vidláková, Pavlína ; Špaček, Jan ; Vítová, Lada ; Hermanová, Monika ; Fojta, Miroslav
Document type: Papers
Conference/Event: Moderní elektrochemické metody /38./, Jetřichovice (CZ), 20180521
Year: 2018
Language: eng
Abstract: Coniplexes of osmium tetroside with nitrogen ligands (Os,L e.g.. with 2,2'-bipyridine (bpy) find application in redox labelling of DNA.. probing of DNA structure, and in studies of DNA interaction with other molecules Os,L preferentially react with pyrimidines in single strand DNA producing electroactive adducts Primary reaction site for Os,L is C=C double bond in pyrimidine nucleobases. In this contribution we introduce a new two-step technique of DNA modification with Os,bpy, consisting in enzymatic construdion or DNA beating butyl acrylate (BA) moieties attached to uracil or T-deaza adenine, followed by chemical modification of a reactive C=C double bond in BA residue.
Keywords: Butyl acrylate; Chemically modified DNA; Osmium tetroxide complexes; Voltammetry
Project no.: GA15-08434S (CEP), GBP206/12/G151 (CEP), EF15_003/0000477
Funding provider: GA ČR, GA ČR, GA MŠk
Host item entry: XXXVIII. Moderní elektrochemické metody. Sborník přednášek, ISBN 978-80-905221-6-9

Institution: Institute of Biophysics AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0297779

Permalink: http://www.nusl.cz/ntk/nusl-396384


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