Original title: Analysis of Denatured PCR Products Modified with 7-deazapurine Bases at Hanging Mercury Drop Electrode
Authors: Dudová, Zdenka ; Špaček, Jan ; Havran, Luděk ; Pivoňková, Hana ; Fojta, Miroslav
Document type: Papers
Conference/Event: Moderní elektrochemické metody /36./, Jetřichovice (CZ), 20160523
Year: 2016
Language: eng
Abstract: 7-deazapunines are synthetic analogues of natural purine nucleobases which can pair with pyrimidines, retaining pairing complementarity of their parent purines. There is replaced N7 atom by CH group in 7-deazapurines so that DNA modified with them don't participate in Hoogsteen basepairing and don't form alternative structures. Here is a study of 7-deazapurines incorpotated into DNA measured at HMDE by CV and ACV. While 7-deazaadenine was reduced at the HMDE, giving rise to a similar irreversible cathodic peak as the natural adenine, 7-deazaguanine didn't yield any peak analogous to the peak G due to Guanine, in Agreement with a loss of corresponding redox in 7-deazaguanine.
Keywords: dna; hmde; pcr; Voltammetry
Project no.: GBP206/12/G151 (CEP)
Funding provider: GA ČR
Host item entry: XXXVI. Moderní elektrochemické metody. Sborník přednášek, ISBN 978-80-905221-4-5

Institution: Institute of Biophysics AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0297874

Permalink: http://www.nusl.cz/ntk/nusl-396402


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