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Structural analysis of the interaction interface between an antibody and a protein epitope
Moravec, Jan ; Krejčíř, Radovan (referee) ; Müller,, Petr (advisor)
The diploma thesis deals with the study of the mouse monoclonal antibody EEV1-2.1, which was created in the RECAMO laboratories by immunization of mice with the Hsp90 protein and subsequent fusion of splenocytes with a mouse myeloma line. The theoretical part of this work focuses mainly on the study of antibodies, especially their structure, genetics and the description of monoclonal antibodies. In this part of the work, you can also find a cross-section of modern biotechnological trends that are currently used for the production of antibodies. CHO cells and tetracycline inducible systems are also described. The aim of the thesis was to characterize the mentioned antibody. Using bioinformatics methods and prediction software to predict and describe its structure, especially then to analyze hypervariable CDR regions. Another goal was to clone the light and heavy chains of the antibody using the Gateway method. The final step was the creation of a tetracycline inducible system for efficient production of the antibody itself. The experimental part of the work initially deals with the isolation of the RNA sequence of the antibody using commercial kits. Next, the method of amplification of the given sequence using PCR with reverse transcription is described. The following is a description of the Gateway cloning method, which allows convenient insertion of the selected gene into the target vector. The basic bioinformatics methods used to study the structure of antibodies are also explained, as well as the prediction of a more complex 3D structure of the antibody, including possible interactions with the HSP90 protein. This section describes the methods of transfection of ExpiCHO cells and inducible antibody production using the tetracycline inducible system. The thesis led to the successful cloning of the light and heavy chain of the antibody and the subsequent insertion of the expression vector into the production ExpiCHO cells. The expression system based on induction by the addition of doxycycline was then successfully tested by several methods and the results show that the inducible system is not only functional, but can even lead to increased production of monoclonal antibody compared to conventional methods.
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Media Law and Media Capture in Slovakia and the Czech Republic after the year 2000
Hanák, Peter ; Moravec, Václav (advisor) ; Kroupa, Jiří (referee) ; Višnovský, Ján (referee)
This dissertation connects theories and notions of media and democracy, oligarchization of media, media capture, and media systems with the economic theory of competition law. Slovak and Czech media laws are analyzed in a comparative legal analysis. Pluralism as the core value in all of the above-mentioned theories is researched. Novel historical periodization of the 1989 - 2020 era is proposed, as these 30 years are framed as a permanent transition of the media system of three distinct types. The Herfindahl-Hirschman index proves that the Slovak and Czech media markets are concentrated or oligopolistic, and this trend was strengthened in the last decade of the examined era. The new tool, the Power of Media Owners (POMO) indicator, is invented to show the strength of media oligarchs across platforms. This proves inefficacy of the cross-media ownership regulations, and it is used to measure the level of media capture. Fourth model of media systems, the non-pluralist authoritarian Eastern Oligarchic Captured model is described and the situation in Slovakia and Czech Republic is compared to it, concluding that both examined countries are in fact closer to more established Polarized Pluralist Mediterranean model, which is framed as a half-way to the Eastern model. Conclusions reveal which changes in...
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Disinformation narratives in the Czech cyberspace in regarding the vaccination against Covid-19
Hospodárová, Klára ; Moravec, Václav (advisor) ; Miessler, Jan (referee)
The bachelor's thesis "Disinformation narratives in the Czech cyberspace in regarding the vaccination against COVID-19" in the context of the phenomenon of misleading, false or pseudo news deals with the way of the news media informing in the Czech environment combined with interpretation of not only these messages by non-professional social network users. The aim of the thesis is to find out whether there is a real correlation between professional and nonprofessional content in the same relevant period. In the theoretical part, emphasis is placed on a sufficient and, above all, comprehensible definition of terms related to the issue of information disorders, including the ordering of individual terms. In the first part of the work, facts are based on relevant sources of internal and foreign literature. In the second part of the work, I present the research background, including hypotheses and research questions. The whole thesis is based on a qualitative research method, which focuses on the analysis of articles produced by professional journalism in contrast to the posts available on the social network Facebook, whose authors are users from the general public, i.e., nonprofessional users. The results are summarized in the conclusion, which evaluates the course and knowledge gained in the...
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Vlastnosti a regulace objemově-závislých aniontových kanálů u astrocytů.
Harantová, Lenka ; Anděrová, Miroslava (advisor) ; Moravec, Jan (referee)
Animal cells need to preserve constant volume in the face of osmolarity perturbations to function properly. To regain their original volume after hyposmotically induced swelling, most cell types extrude intracellular electrolytes and organic osmolytes accompanied by osmotically driven water. This process is termed regulatory volume decrease and is ensured by various ion channels and transporters. Recently, much attention has been focused on the ubiquitous volume-regulated anion channels activated by cell swelling. VRACs are moderately outwardly rectifying with intermediary conductance, permeable to inorganic anions and organic osmolytes and sensitive to broad-spectrum anion channels blockers. Functional properties of VRACs in astrocytes are particularly interesting, because many brain pathologies, such as ischemia, traumatic brain injury or hyponatremia, are associated with marked astrocytic swelling and VRACs could thus constitute a possible target for therapy of cerebral edema. Furthermore, VRACs are thought to play a role in cell cycle progression, cell migration, apoptosis and intercellular communication. Despite intensive research, VRACs molecular identity and mechanism of their activation and regulation are still unclear. This work summarizes known facts about VRACs, accentuating their...
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Neurosteroid modulation of ligand-gated ion-channel activity
Krausová, Barbora ; Vyklický, Ladislav (advisor) ; Moravec, Jan (referee)
The term neurosteroids refers to steroids that are synthetized in the nervous tissue from cholesterol or steroidal precursors from peripheral sources. These compounds affect the neuronal excitability by modulating the function of some ligand-gated ion channels. NMDA (N methyl D aspartate) receptors are glutamate gated ion channels involved in excitatory synaptic transmission, synaptic plasticity and excitotoxicity. GABAA ( aminobutyric acid type A) receptors mediate most of the inhibitory synaptic transmission in the mammalian brain and are targeted by many clinically important drugs. Function of NMDA and GABAA receptors can by affected by neurosteroids, both positively and negatively. The aim of this work is to summarize the current knowledge about the neurosteroid effects on the function of GABAA a NMDA receptors and suggest the physiological role and the potential therapeutic use of the neurosteroids as a regulator of some functions of the central nervous system.
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Option Pricing
Moravec, Radek ; Hurt, Jan (advisor) ; Cipra, Tomáš (referee)
Title: Option Pricing Author: Radek Moravec Department: Department of Probability and Mathematical Statistics Supervisor: doc. RNDr. Jan Hurt, CSc., Department of Probability and Mathematical Statistics In the present thesis we deal with European call option pricing using lattice approaches. We introduce a discrete market model and show a way how to find an arbitrage price of financial instruments on complete markets. It's equal to the discounted value of future expected cash flow. We present the binomial option pricing model and generalize it into multinomial model. We test the resulting formula on real market data obtained from NYSE and NASDAQ. We suggest a parameter estimate method which is based on time series of historical observations of daily close price. We compare calculated option prices with their real market value and try to explain the reasons of the differences. 1
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Point processes on linear networks
Moravec, Jan ; Prokešová, Michaela (advisor) ; Pawlas, Zbyněk (referee)
The central theme of this thesis is the theory of point processes on linear net- works, in particular two kinds of the network K-function. The first part is devoted to the theory of stationary point processes in the plane, including the K-function and its estimator. The second part is concerned with the theory of point proces- ses on linear networks. There is defined the Okabe-Yamada network K -function and its estimator, the geometrically corrected network K-function, including its estimator, and there are explained their theoretical properties. In the third part we examine the ability of these two kinds of the network K-function to detect clustering or regularity in point processes on linear networks. There is explained the envelope test, the refined envelope test and the deviation tests. The software environment R with library spatstat is used for simulations.
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Ecology,ethology and variability of european green lizard Lacerta viridis in Natural reservation Tiché údolí
Chmelař, Jan ; Rehák, Ivan (advisor) ; Moravec, Jiří (referee)
The european green lizard, Lacerta viridis is a critically endangered reptile species which is in the region of Bohemia present only in relict isolated populations. These populations are closely linked to slopes with a southern exposition in deeply engorged river valleys. These valleys exhibit a unique climatic effect also known as the river phenomenon which allows numerous animal and plant species to survive far beyond their standard northern range. The chosen locality in Tiché údolí is a subject to long-term conservational management and the studied lizard population seems to reflect its effectiveness. This study is aimed to evaluate variability, ecological and ethological characteristics of lizards within the locality and is focused on changes on the population level. Key words: European green lizard, Lacerta viridis, Tiché údolí, river phenomenon, variability, ecology, ethology, population characteristics Abstrakt Ještěrka zelená, Lacerta viridis, je kriticky ohrožený druh plaza, který se na území Čech vyskytuje pouze v reliktních a izolovaných populacích. Ty jsou úzce spjaty se svahy v hluboko zaříznutých říčních údolích. Tato údolí vykazují unikátní klimatický efekt, známý též jako říční fenomén, který umožňuje výskyt četných živočišných i rostlinných druhů i za severní hranicí jejich běžného...
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