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Determination of L-tryptophan in food supplements in high performace liquid chromatography
Pešková, Pavlína ; Kalíková, Květa (advisor) ; Kubíčková, Anna (referee)
This bachelor's thesis deals with optimalization of chromatographic conditions for determination of tryptophan by HPLC method with UV detection. The amount of tryptophan has been determined in two food supplements. To find suitable conditions for the determination of tryptophan, the influence of the composition of the mobile phase on analyte retention and peak symmetry was tested in two different chromatographic modes - reversed-phase mode using the Atlantis dC18 column and mixed-mode using the Atlantis PREMIER BEH C18 AX column. Specifically, the effect of the type and amount of organic modifier (acetonitrile/methanol), the effect of the pH of the ammonium acetate buffer (4,7;5,9;7,0) and its concentration (5mM/10mM) in the mobile phase was tested. Based on the obtained data, the optimal chromatographic conditions were selected (in terms of analysis time and peak symmetry). In the optimized conditions selected validation parameters such as repeatability, reproducibility, limits of detection and quantification were determined. The optimized conditions were as follows: mixed-mode Atlantis PREMIER BEH C18 AX column, mobile phase acetonitrile/5mM ammonium acetate, pH 7,0 10/90 (v/v), separation temperature 25 řC, flow rate 1 ml/min and UV detection at 254 nm. Under these conditions, the tryptophan...
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Employment and Study Opportunities among People with Intellectual Disability in area of Domažlice region
Kukolová, Natálie ; Štrinclová, Marta (advisor) ; Kubíčková, Anna (referee)
This Bachelor thesis is focused on the support of people with intellectual disability living in the area of Domažlice district (Pilsen Region). The thesis consists of a theoretical and an empirical part. The theoretical part is research of specialized literature and it contains fundamental information about the life of people with intellectual disability. The first chapter defines essential terms concerning intellectual disability and it characterizes people with this disability. The second chapter is focused on the education of people with intellectual handicap and it is divided into subchapters, concerning preschool, primary and secondary education. Then follows the third chapter with attention to the employment of people with intellectual disability. It is mainly focused on the right to employment and its importance. This chapter also contains specific options of the support for people with intellectual disability in the labour market. The last chapter includes information concerning non-profit organizations and volunteering, because these are very important aspects in lives of people with intellectual handicap. The main goal of the empirical part of this thesis is creating a comprehensive overview which concerns the providing of social services and the options of education and employment of...
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Wellbeing support for children in a children's home
Pražáková, Ellen ; Kubíčková, Anna (advisor) ; Štrinclová, Marta (referee)
The thesis entitled The Well-being of children in children's homes starts with description of characteristics and legislation of institutional education, specifically in children's home. The thesis introduces negative influences in childhood and tries to describe their origin and influence on the child's life. The thesis contains a relatively new concept of well-being, which characterizes important areas of life. The aim of the bachelor thesis is to describe and characterize how the competencies related to areas of well-being are developed directly in the children's home and what are the barriers to fulfilling well-being. Nine respondents who work and interact directly with children in a children's home described the development and barriers to the development of these competencies. All respondents agreed that a big barrier to fulfill well-being is an unsuitable environment and rooms and other spaces where children live. Furthermore, they often mentioned insufficient social interaction with life outside the children's home, mental and psychological disorders that prevent children from further development, the absence of role models and traumatic experiences that children carry from the time they were in their families. According to the respondents, the fundamental barrier to the development of...
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DEVELOPMENT AND OPTIMIZATION OF PACKING CHROMATOGRAPHIC COLUMNS
Černá, Kateřina ; Kozlík, Petr (advisor) ; Kubíčková, Anna (referee)
The aim of this thesis was to develop and optimize the packing of a new series of chromatographic columns called Astra with subsequent analytical measurements to determine the quality of optimized packing processes, the quality of the columns and to investigate the behavior of stationary phases. The packing processes for the following columns were developed and optimized - Astra C18 HE, AQ and DM 5 µm (250/150/100×4,6 mm, 150/100×3,0 mm), Astra C18-HE 3 µm (250/150/100×4,6 mm) , 150/100/50×3,0 mm, 150/100/75/50×2,1 mm), Astra AQ 3 µm (150/100×4,6 mm, 150/100/50x3,0 mm, 150/100/75/50×2,1 mm), Astra DM 3µm (150/100×4,6 mm, 150/100x3,0 mm, 150/100/50×2,1 mm) and for zwitterionic columns 3µm (150×4,6 mm, 100×2,1 mm). During the development of the packing processes, the following packing parameters were optimized. Composition of the suspension solution (slurry) - cyclohexanol:chloroform (1:10) for SF C18, AQ, DM and glycerin:methanol (1:3) for zwitterionic SF; concentration of the packing suspension; column pre-filling method - pre-filling with packing liquid (methanol); PID parameters, the values P = 3000, I = 100 and D = 100 were chosen; width of the pre-column, which must be equal to or greater than the inside diameter of the column; packing pressure and time; the column hardware type was chosen...
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Analysis of historical pharmaceutical preparation containing chicory from the 18th century
Lener, Tomáš ; Nesměrák, Karel (advisor) ; Kubíčková, Anna (referee)
A historical pharmaceutical preparation - chicory extract - from the 18th century whose authenticity was investigated was qualitatively analyzed using HPLC with UV/VIS and MS2 detection. A current dry chicory root was used as a source of selected chicory chemotaxonomic markers and an indicator of the success of extraction with different solvents. The authenticity of the historical extract was proved by a water solubility test. A VIS spectrometric determination of inulin was used and the inuline content was determined to be 52.4% in the current dry chicory root and 7.45% in the historical extract. The detection wavelength 254 and 320 nm was selected from measured UV/VIS spectra of selected standards and the literature. The authenticity of the historical chicory extract was proven by tandem mass spectrometry, and, moreover, arteincultone - a new chicory chemotaxonomic marker - was identified. Key words: HPLC-MS, HPLC-UV/VIS, UV/VIS Spectrometry, Chicory, Historical extract, Chicory root, Arteincultone
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Electrophoretic determination of oxidized and reduced form of glutathion in plant material
Durychová, Eva ; Křížek, Tomáš (advisor) ; Kubíčková, Anna (referee)
This work is based on a previously published method for the determination of twenty proteinogenic amino acids by capillary electrophoresis with contactless conductivity detection and deals with possibility of its use for the determination of oxidized (GSSG) and reduced (GSH) forms of glutathione with amino acids. The theoretical part firstly deals with brief introduction of the properties and importance of glutathione and its protection against autooxidation, especially by alkylation with N-ethylmaleimide (NEM), which binds to GSH to form its alkylated form (GSH-NEM). Then, a brief summary of the available methods for determination of glutathione is given, with a focus on the already developed capillary zone electrophoresis methods. Finally, it briefly presents the electrophoretic method for the determination of twenty proteinogenic amino acids on which this work is based. In the experimental part, the integration of the determination of both forms of glutathione into the method for the determination of proteinogenic amino acids is presented. First, a simplified, less time consuming method was tested in shorter 50.0 cm capillary without hydroxyethylcellulose, where both forms of glutathione were separated at baseline within 10 minutes. Relative standard deviations of peak areas (GSH 2.1%; GSSG...
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The study of the electrochemical and chemical oxidative degradation of salicylic and acetylsalicylic acid
Tonnerová, Barbora ; Kubíčková, Anna (advisor) ; Křížek, Tomáš (referee)
The aim of this Thesis was to study the degradation of salicylic acid by the electrochemical oxidation. The electrochemical oxidation of salicylic acid was compared to the electrochemical oxidation of acetylsalicylic acid. Two electrochemical cells were tested - batch cell and thin- layer flow-cell. The newly developed and validated method of ultra-high performance liquid chromatography has been used to analyse the degradation products, salicylic acid and acetylsalicylic acid. The optimal analysis was made by the Kinetex column C18 (2,1 x 100 mm; 1,7 µm) and mobile phase with two components - acetonitrile and water with addition of 0,1% formic acid. These two components of the mobile phase were mixed by the gradient program from 10 % to 60 % (v/v) acetonitrile. The mobile phase flow was set to 0,3 ml min-1 and the volume of injection was 2 µl. The detection was performed by photodiode array detector at the wavelength 240 nm. Total time of the analysis was 11 minutes. The electrochemical degradation has been studied by an anodic oxidation in a flow cell with a boron-doped diamond (BDD) anode and a stainless-steel cathode. The samples of salicylic acid and acetylsalicylic acid was dissolved in the ammonium acetate, concentration 200 mmol dm-3 at pH=4,0. The 20 % oxidation rate of salicylic acid was...
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