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Business process modeling in a context of customer communication
Pokorná, Martina ; Šisler, Vít (advisor) ; Slussareff, Michaela (referee)
(in English): The basis of this diploma thesis is to describe business process modelling and its current use in IT companies. The subject is to determine if respondents use process modelling in their companies or if they find it unsuitable. In the theoretical part, modelling in the context of information science, new media, and some models of communication are briefly presented. An important part of the theory is focused on the chapter of cognitive modelling, as a basic approach for creating process models. It follows an introduction of process modelling and a current market overview in this industry. The practical part maps the use of modelling in companies. Methodology is presented as first followed by data analysis. The research uses questionnaire survey for data collection, for qualitative data processing applies the grounded theory. Finally, the thesis evaluates the current state of modelling on the Czech market and defines the strengths and weaknesses of the process modelling.
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Comparison of the rate of evolution in genes located on reptile sex chromosomes and autosomes
Kuldanová, Kateřina ; Johnson Pokorná, Martina (advisor) ; Reifová, Radka (referee)
According to the "faster-X effect", X-linked genes and Z-linked genes evolve more quickly than autosomal genes. This theory is one of the currently intensively studied topics in evolutionary research. However, performing high quality tests is difficult because the results are influenced by several factors - the effective size of the population of the gonosome, sexual selection, the dependency of mutation rate and selection on sex, and the mechanism of dosage compensation. Conservation of genes and possible differences between rates of evolution of autosomes also play a role and not all studies take this fact into account. This study shows some of the difficulties of paired comparisons of dN/dS ratios traditionally used to test faster-X or faster-Z effects and introduces the basis of a new method of comparison of the rate of evolution (CREC) based on relative genetic distances between three species. The CREC method reduces the influence of conservation of genes on results and is more applicable for testing faster-X or faster Z effects in such species where two species without homologic gonosomes can be found for comparison. In means of the development of this method, the faster-Z effect was tested on a dataset of 9 autosomal and 13 Z-linked genes in the six-striped long-tailed lizard (Takydromus...
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Neo-sex chromosomes and their evolutionary significance
Košátko, Prokop ; Král, Jiří (advisor) ; Johnson Pokorná, Martina (referee)
Neo-sex chromosomes arise due to rearrangements between ancestral gonosomes and autosomes. Neo-sex chromosomes are valuable systems for the study of sex chromosome evolution. It is possible to use them for analysis of processes driving the formation of differentiated sex chromosomes, especially suppression of recombinations and alosome degeneration. The most important rearrangements forming neo-sex chromosomes are Robertsonian and reciprocal translocations. Speciation events are important consequences of birth of neo-sex chromosomes. The presented study is focused on the evolution of neo-sex chromosomes in drosophilids, muntjacs, and monotremes. Neo-sex chromosomes emerged in many Drosophila species. The genome of D. albomicans carries the youngest known neo-sex chromosome system, which arised only one hundred thousand years ago. Information from research into Drosophila neo-sex chromosomes underlines the importance of Muller's ratchet and background selection at the beginning of sex chromosome differentiation and hitchhiking and deleterious mutations at the end of this process. Genomes of muntjacs carry young and fast evolving neo-sex chromosomes. In several muntjac species, neo-sex chromosomes form a considerable part of the genome. The neo-sex chromosome systems of monotremes consist of many...
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Use of cytogenetical methods in taxonomy of Arachnida (Arachnida)
Alaverdyan, Argam ; Šťáhlavský, František (advisor) ; Johnson Pokorná, Martina (referee)
This bachelor thesis deals with the use of cytogenetic methods in taxonomy of Arachnida. To understand this matter, we need to analyse available information about inter and intraspecific karyotype variability of individual orders. At the beginning of the thesis there is a description of cytogenetic methods used with Arachnida.They are used to gather the karyotype's fundamental information, such as the diploid number of chromosomes, chromosome morfology and possible occurence of sex chromosomes. Main part of the thesis is focused on describing karyotypes of selected orders (Amblypygi, Palpigradi, Pseudoscorpiones, Scorpions, Opiliones and Araneae) and considering the convenience of use of cytogenetic methods for their taxonomy based on these information. The thesis includes current number of described genera (species) of the orders, as well as a number of cytogenetically analyzed genera (species) for comparison.
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Alternative use of Szondi test as a diagnostic tool
Pokorná, Martina ; Bahbouh, Radvan (referee) ; Niederlová, Markéta (referee)
The rigorous thesis analyzes the Szondi test and its theory in the light of current knowledge. Its aim is to examine the potential of this diagnostic method and to consider the possibilities of its application. On the basis of the free-association research, the direction of interest has divided into three studies. The first of them verifies the relationship of Szondi's theory with personality characteristics, that are attributed to individual photos (stimulus material) on the scale of the semantic differential. In most cases, the results confirm significant differences between the factors. In the second study, the narrative approach is selected, it means "telling stories" method will be used for discover the projective potential. Qualitative analysis indicates, that stories have some similarities - in the context of given factors, however, they almost never follow Szondi's assumptions. The data does suggest, that Szondi test has a potencial to insight into the important topics of the respondent's life. The third study examines the relationship between the Szondi test and selected personality characteristics measured by Szondi test. The research confirmed the connection between a choice of photographs and some of detected personality qualities. KEY WORDS: Szondi test, projection, face perception,...
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Viability of probiotic bacteria in some foods
Pokorná, Martina ; Bokrová, Jitka (referee) ; Márová, Ivana (advisor)
This bachelor thesis is focused on viability od probiotic bacteria in some foods and looking for optimal food and beverages, that exhibited a minimal negative effect on the growth and viability of probiotic bacteria. Lactobacillus acidophilus, Bifidobacterium breve and commercially complex of probiotics were tested. In the practical part, probiotics were incubated in the environment of selected types of model and real foods. Further, incubation in model gastrointestinal juices to simulate human digestion was performed. Model foods with a moderate value of concentrations of sugar, protein and alcohol were shown the best alternative for growth of bacteria and their lowest mortality. For salt, the smallest concentration was shown as the most suitable. The chicken broth, chicken and beef meat were evaluated as the best environment for the capsule with complex probiotics. The vegetables and vegetable broth were stated like the best for Lactobacillus acidophilus and Bifidobacterium breve. According to the preliminary results we can conclude that the probiotics should be preferably consumed during meals and with the sufficient amount of liquids for the activation of all cells.
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Evolution of sex-determining mechanisms and genomes in squamate reptiles (Reptilia: Squamata)
Pokorná, Martina ; Kratochvíl, Lukáš (advisor) ; Marec, František (referee) ; Vyskot, Boris (referee)
Evolution of sex determining mechanisms in squamate reptiles (Reptilia: Squamata) Martina Pokorná Ph.D. thesis Abstract This Ph.D. thesis is focused on the evolution of sex determining mechanisms and genomes in squamate reptiles. It is based on three published articles and two manuscripts. The evolution of sex determining mechanisms, sex chromosomes and genomes, and their organisation, was studied on a wide phylogenetic scale of the whole group of squamate reptiles and some lineages of other Sauropsids, as well as on the small phylogenetic range as a detailed comparative study inside individual lineages of squamates. This thesis is based upon the use of classical cytogenetic methods, methods of molecular cytogenetic (especially fluorescent in situ hybridisation) and the results were analysed using phylogenetic approaches. The results and outputs of this study represent an important contribution to the general knowledge of the principals of sex determination and the evolution of these phenomena not only in squamate reptiles but also in the whole group of amniotes. Using the results obtained during the work on this thesis we can conclude that sex chromosomes evolved in particular lineages of amniotes independently. This origin was in some cases followed by accumulation of microsatellite sequences on sex...
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Cytogenetics of bed bugs (Cimicidae) as a model representative of true bugs (Heteroptera)
Sadílek, David ; Johnson Pokorná, Martina (referee) ; Vilímová, Jitka (advisor)
Cytogenetics of bed bugs (Cimicidae) as a model true bugs (Insecta: Heteroptera) The thesis provides current opinions about a phylogeny of bed bugs, family Cimicidae, and their classification within the order Heteroptera. There are briefly summarized cytological data about the order Heteroptera, known karyotypes of the cimicid subfamilies and introduction to cytogenetics of species Cimex lectularius Linnaeus, 1758. Heteroptera species differ from other organisms by holokinetic chromosomes, a character, in which quite frequently take place chromosomal fragmentation and fusion. Several families posses smaller pair of m chromosomes, which behave during meiosis differently from the other chromosomes. Diploid chromosomal number is extremely variable, it ranges from four (family Belostomatidae) to 80 chromosomes (family Miridae). The behavior of chromosomes in the family Cimicidae is very specific. Sex-chromosomes univalents are connected by thread-like collochores and form so-called pseudobivalents during achiasmatic post-reductional meiosis. Rare polymorphism in a number of X chromosomes occurs in Cimex lectularius populations. The diploid karyotype always consists of 26 autosomal chromosomes and at least two but up to 15 X chromosomes and one Y chromosome. The number of X chromosomes can be variable...
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Karyotypic evolution in eye-lid geckos (Squamata: Eublepharidae), a group with different modes of sex-determination
Pokorná, Martina
Chromosome rearrangements represent one of the most striking phenomena related to speciation. Nevertheless, their importance for other evolutionary processes is not well understood yet. Within some groups we observe even variability in the presence or absence of differentiated sex chromosomes. Such variability has a crucial role for our comprehension of general principles regarding the evolution of sex determining mechanisms. From this point, the family Eublepharidae is a very interesting model group as it includes species with both genetic sex determination and temperature-dependent sex determination. The investigation of the karyotype composition as well as of the structure and homology of sex chromosomes within such taxa could bring valuable information on the general evolutionary processes leading to the differentiation of karyotypes and sex chromosomes. In this work the description of karyotypes in several selected species of the family Eublapharidae was done by available cytogenetic techniques. I have examined following species: Coleonyx brevis, C. elegans, C. mitratus, C. variegatus, Eublepharis angramainyu, E. macularius, Goniurosaurus araneus, G. lichtenfelderi,G. luii, G. splendens, Hemitheconyx caudicinctus, Holodactylus africanus. It was found that the karyotypic differentiation among...
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