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Nanospray processing of silver nanoparticles for formation of dried deposits
Týčová, Anna ; Jonas, Vladimír ; Přikryl, Jan ; Kotzianová, A. ; Velebný, V. ; Foret, František
Nanospray transfers liquid into an aerosol via electrostatic forces created between an emitter and a counter electrode. Herein, we present compact laboratory-made instrumentation for nanospray-processing of silver colloid. The instrumentation is based on the pressurized polysulfone chamber fixing a disposable vial with an immersed platinum electrode and a long fused silica capillary supplying the sprayed liquid to the emitter. The combination of low flow rates (70 nL/min) with a sharp emitter, fabricated on a 3D printed grinding station, resulted in a fine aerosol. The low volume of released droplets allowed full evaporation of water during their flight towards the counter electrode without any need for drying gas or the addition of volatile solvents. The constructed device was successfully used for the deposition of water-free silver colloid without any requirement of its pretreatment. The deposition of completely dried nanoparticles on planar substrates eliminated undesirable coffee ring effect and deposits of increased homogeneity could be obtained. Electron microscopy confirmed no significant changes in the character of nanospray-processed nanoparticles. Finally, we also investigated several approaches for the improvement of the surface density of nanoparticles on the substrate at preserved time scale.
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Isotachophoretic determination of ketone bodies and lactate in cattle serum
Vyskočilová, V. ; Pechová, A. ; Pantůčková, Pavla
The aim of the work was the optimization of isotachophoretic determination of lactate and ketone bodies (beta-hydroxybutyrate and acetoacetate) for veterinary medicine. The measurement of these metabolites is very important for the evaluation of metabolic diseases of cattle. The complete separation of these three analytes together with other possible blood components was achieved using 10 mmol/l hydrochloric acid with beta-alanine as a leading electrolyte at pH = 3.15 and 10 mmol/l sodium propionate as a terminating electrolyte. Limits of detection determined from the standard mixture for acetoacetate, lactate and beta-hydroxybutyrate were 3.75 mu mol/l, 3.96 mu mol/l and 7.46 mu mol/l, respectively.
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Computer assisted development of a pneumatic electrospray nebulizer
Járvás, G. ; Szigeti, M. ; Grym, Jakub ; Foret, František ; Guttman, A.
In this study we demonstrate how computer simulations can assist the development of a novel microfabricated pneumatic electrospray nebulizer. The microdevice under consideration is designed for electrospray mass spectrometry interfacing without the need to fabricate an electrospray needle. This new interfacing strategy requires comprehensive evaluation of the differently designed chip layouts. The flow evaluation was supported by computational fluid dynamics simulations. It was proved with experimental validation of the simulation results that the developed models were practical tools for the numerical investigation of the separations-ESI-MS coupling.
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Porovnání gradientového měření a modelování depozičního toku ozonu do smrkového porostu v lokalitě Bílý Kříž (Moravskoslezské Beskydy)
Zapletal, M. ; Chroust, P. ; Večeřa, Zbyněk ; Mikuška, Pavel ; Cudlín, Pavel ; Urban, Otmar ; Pokorný, Radek ; Czerný, Radek ; Janouš, Dalibor ; Taufarová, Klára ; Marek, Michal V. ; Hůnová, I. ; Černikovský, L. ; Medlen, A.
Porovnání naměřených a modelovaných depozičních rychlostí ozonu do smrkových lesů v lokalitě Bílý Kříž. Odhad denních změn depozičního a stomatálního toku ozonu do smrkových lesů.
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