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The methodology for evaluation of environmental education programs
Činčera, Jan
The methodology may be used by public administration officials who evaluate both formal and informal environmental education programs for various reasons (incl. making decisions on the distribution of funds to support formal and informal environmental education programs)., The results can than be used by those who implemented the program to improve it or by its external evaluators such as the donor to asses its efficiency. The aim of the presented methodology is to define the basic terminology for evaluating environmental education programs, describe the basic stages of the evaluation cycle, characterize the basic alternative procedures for evaluating programs, and finally, to identify the basic requirements for evaluation research. Development of the methodology is based on the assumption that the evaluation research in the medium term will become an integral part of environmental education programs. To achieve this objective requires that officials are familiar with the theme and gain a basic understanding of the opportunities and needs of the assessment procedures.
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Methods for the detection of Paenibacillus larvae and Melissococcus plutonius using real-time PCR
Hrabák, Jaroslav ; Vlková, Kateřina ; Erban, Tomáš
The methodology is intended for diagnostic laboratories focused on bee infections. Detection of the causative agents of American foulbrood (Paenibacillus larvae) and European foulbrood (Melissococcus plutonius) is essential to prevent the spread of those highly contagious infections of a honey bee (Apis mellifera) diseases. In case of the suspicion, an examination of bee colonies in protective zones of the disease is indicated. The second possibility of use is the confirmation of the infection based on the detected clinical symptoms in the bee colony. The main target user in the Czech Republic are laboratories investigating animal diseases for the State Veterinary Administration (SVS) (accredited laboratories) in case of suspected disease and investigations in protective zones. Other target users are laboratories investigating bee colonies for reasons of preventive surveillance based on the request of beekeepers. The PCR method enables fast and reliable detection of P. larvae and M. plutonius from different samples taken from the bee colony. Compared to classic microbiological techniques, it allows to provide a result within a few hours of receiving the sample in the laboratory. In this way, it is possible to ensure, above all during the period of intense flight activity of bees, early intervention in bee colonies suspected of the occurrence of the mentioned diseases and to prevent the spread of the disease to nearby habitats of bee colonies.
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Methodology for increasing the capacity of local actors for sustainable development of the region
Dlouhá, Jana ; Pospíšilová, Marie ; Petiška, Eduard ; Dlouhý, Jiří ; Macháčková Henderson, Laura
An analysis of the needs of rural and small communities in terms of sustainability shows that the preconditions for initiating change are, among others, in the social sphere: these include social capital (trust, developed relationships between actors, joint activities) and education appropriate to the conditions of the region (shaping local identity). The Methodology proposes how relationships and cooperation in a given place can be shaped and subsequently used to improve the quality of life in all its dimensions - social, environmental and economic. It shows practices for shaping local culture and lifestyle, presents the experience of the Movement for Change, recapitulates the principles of social entrepreneurship, and refers to existing forms of cooperation in community projects. It shows that opportunities for education, especially based on locally embedded learning, are lacking so that a shared understanding of sustainability principles in the regions to ensure a general awareness of local UR resources and opportunities is missing.
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Methodology of development of case studies for Higher Education and as Open Educational Resources (OER)
Dlouhá, Jana ; Dlouhý, Jiří ; Kapitulčinová, Dana
The methodology of development of regional case studies of Sustainable Development for Higher Education is aimed at the promotion of the use of practical cases in teaching; it should lead to the development of research tools relevant to local environmental issues and issues of sustainable development of the region, and thus emphasizes the importance of the regional context and specifics. The publication of the cases in the form of open educational resources (OER) enables access to these materials for regionally oriented education at earlier stages of the education system, and can be used for the presentation of regional specifics, and may also serve to promote dialogue concerning the development of the territory between the different social actors. The methodology outlines the procedure for creating educational case studies and shows how they are used in university teaching. Because the methodology is based on qualitative research methods working with direct experience, discussion also addresses the ways and means of creating a knowledge base as an open educational resources (OER), to facilitate the accumulation of this experience. The methodology takes into account the requirement for continuity of learning at different educational levels and gives a brief overview of similar approaches applied at lower levels of the education system; it also shows how the results can be applied in practice. The methods of case study development and use are based on the authors' own teaching experience, and on its theoretical reflection. The methodology clearly summarizes the assumptions for teaching sustainable development issues from a regional perspective; it offers the possibility of linking university teaching and research with practice, which should lead to an increased competence in addressing local issues of sustainable development in cooperation with universities.
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HPLC in quality control of food supplements based on goldenrod and containing rutin and chlorogenic acid
Kotyková, Klára ; Šatínský, Dalibor (advisor) ; Chocholoušová Havlíková, Lucie (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Klára Kotyková Supervisor: prof. RNDr. Dalibor Šatínský, Ph.D. Title of the diploma thesis: HPLC in quality control of food supplements based on goldenrod and containing rutin and chlorogenic acid In this diploma thesis, a high-performance liquid chromatography (HPLC) method was developed and validated for the separation and determination of chlorogenic acid, 3,5- dicaffeoylquinic acid, rutin, quercetin, and kaempferol. This method was applied to the analysis of food supplements based on goldenrod: drops Celík zlatobýl (Valdemar Grešík), Celík obecný BIO (Bylinné kapky), Celík obecný (Bylinné kapky), Zlatobýl (Green Idea), Zlatobýl - tinktura z pupenů (Serafin), Zlatobýl - tinktura z bylin (Serafin), Zlatobýlová směs (Dědek kořenář), Zlatobýl (Inca Botanica), and capsules Celík (Ex Herbis). Furthermore, two food supplements containing rutin (Solgar and Walmark®) and a food supplement Zelená káva (Botanic) containing chlorogenic acid were analyzed. An Ascentis® Express 90 Å AQ-C18 150 × 4.6 mm column with 2.7 µm particles was selected for analysis. A gradient elution with a mobile phase of acetonitrile/0.085% phosphoric acid was used. Detection was performed with a diode array detector at...
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The role of infection of HPV and EBV in the etiology of nasopharyngeal and cervical carcinoma
Machů, Jakub ; Vojtěchová, Zuzana (advisor) ; Španielová, Hana (referee)
Human papillomaviruses (HPV) and Epstein-Barr viruses (EBV) are common DNA viruses that contribute to the development and progression of tumours. These two groups of viruses are associated with more than 600,000 cases of newly diagnosed tumours of different anatomical locations annually. However, carcinogenesis is not a part of their normal productive life cycle. High risk HPV infections are mainly associated with head and neck tumours and anogenital cancers. EBV infections are associated with a range of acute or chronic diseases and tumours, with Burkitt's lymfomas (BL), Hodgkin's lymphomas (HL) and nasopharyngeal carcinomas (NPC) being the most prominent. In recent years, it has been hypothesized that EBV infections may be involved in the development of cervical cancer in woman and that HPV infections may play a role in the development of NPC. The aim of this thesis is to characterize above mentioned groups of DNA viruses and to summarize currently available information about the potential role of these viruses in nasopharyngeal and cervical carcinomas. Key words: human papillomavirus, Epstein-Barr virus, nasopharyngeal carcinoma, cervical carcinoma
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Personality predictors of exercise addiction in competitive sport
Vítková, Tereza ; Mudrák, Jiří (advisor) ; Bezdíček, Ondřej (referee)
Exercise addiction is becoming a recognized concern, particularly prevalent in elite athletic environments where the pursuit of perfection is emphasized. Despite growing awareness of problematic exercise behaviors, research investigating the underlying risk factors remains limited. This study aimed to examine personality predictors of exercise addiction in Czech elite athletes. A sample of 168 competitive athletes, aged 18 to 30 years, completed assessments including the Exercise Addiction Inventory, Sport Motivation Scale-6, the Athletic Identity Measurement Scale, Performance Perfectionism Scale-Sport, and the Neuroticism Scale from Big Five Inventory. In terms of prevalence, the findings revealed that 16.5 % of athletes exhibited problematic signs of exercise addiction, while 76.7 % were potentially at risk with low severity of symptoms. No significant differences were observed between different groups of sports. Using multiple linear regression, the study demonstrated a significant overall effect of personality predictors on exercise addiction. Neuroticism, introjected regulation, and athletic identity were identified as significant predictors of exercise addiction. These findings highlight the importance of psychological factors such as personality traits and motivational regulation in...
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Dispersal and personality
Rišová, Slavomíra ; Frynta, Daniel (advisor) ; Sedláček, František (referee)
Dispersal of animals is an important area of ecology, the study of which is crucial for understanding population composition and dynamics. The acquired knowledge has broad applications in various fields, whether in the efforts to introduce endangered species or, conversely, to suppress the spread of invasive ones. However, dispersal begins at the individual level, so it is first necessary to determine which traits dispersing individuals possess. One possible aspect in which dispersers may differ from residents is their personality. This bachelor thesis provides a summary of literature that focuses on personality predictors of dispersal, comparing personality traits among each other, both within and across three selected taxa: fish, birds, and representatives of mammals - rodents. The literature suggests that in the context of individual personality, besides others, three main types of dispersal exist. In the first type, primarily observed in early stages of life, dispersal of more submissive individuals of territorial fish and rodents is enforced due to aggression from more dominant individuals. However, in birds, it is necessary to secure suitable territory as quickly as possible, leading to the opposite dependence - earlier dispersal of dominant individuals. The second type involves voluntary...
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Virus interaction with the cellular DNA damage response
Lemberková, Eva ; Šroller, Vojtěch (advisor) ; Kadlečková, Dominika (referee)
Maintenance of genomic integrity is an essential mechanism for every cell. Genomic integrity is disturbed by diverse exogenous or endogenous effects influencing the cell and causing damage of its DNA. Cellular mechanisms capable of fixing these disturbances in structure or sequence are indispensable because damaged genetic information can later cause expression of damaged proteins or inaccurate segregation of chromosomes to daughter cells. Therefore, many effective mechanisms for fixing wide range of types of DNA damage have evolved. This thesis focuses mainly on the eucaryotic MRN complex, which plays an important role in detection and repair of double strand breaks. Many viral families try to block these cellular repair mechanisms because they are activated soon after viral infection. One of the reasons for their activation is the resemblance of some viral genomes to the cellular DNA with double strand breaks. Thus, in many cases, the cell ends up inhibiting the life cycle of the virus by attempting to repair viral genomes. However, there are viruses that use cellular repair mechanisms for the replication of their genome, making these mechanisms essential for their own growth. Key words: DNA damage response, homologous recombination, non-homologous end-joining, MRN complex, Adenoviridae,...
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Contract for work in the field of construction
Slámová, Anna ; Macková, Alena (advisor) ; Pohl, Tomáš (referee)
Guardianship in inheritance proceedings Abstract The presented diploma thesis deals with "guardianship in inheritance proceedings". The thesis aims to evaluate the meaning and purpose of the institute of guardianship focused on inheritance proceedings, to describe its legislation and relevant jurisprudence, and answer the question of whether the current legislation on guardianship provides sufficient protection to the rights and interests of persons under special protection participating in court proceedings. The thesis consists of five parts, including an introduction and a conclusion. The first part focuses on inheritance proceedings, its legal regulation, sources, and the proceedings in the context of the legal system. Inheritance proceedings are defined as non-disputed proceedings, and a comparison between non-disputed and disputed proceedings is also given. This part explains the court's jurisdiction, the preconditions, the proceedings' initiation, and the notary's role as a court commissioner. The course of the entire inheritance proceedings and the decisions issued in the proceedings are also described. In the second part, the participation in civil court proceedings is discussed. A general explanation of procedural entities, preconditions for participation such as procedural capacity and capacity to...
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