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Comprehensive chemical analysis of blood coatings
Matěcha, Tadeáš ; Kučková, Štěpánka (advisor) ; Kolář, Karel (referee)
This thesis deals with the comprehensive chemical analysis of blood coatings using mass spectrometry and infrared spectroscopy. The theoretical part focuses first on the history of use, properties, and formulations of blood coatings, then on their composition, which is divided into protein and non-protein components, as well as on selected analytical methods used for their identification. In the experimental part, 41 model samples and 2 real samples are evaluated and discussed. Liquid chromatography with tandem mass spectrometry (nano-LC-ESI-Q-TOF MS) was used to identify the protein component in all samples. Determination of the quantitative abundance of each type of protein additive in the blood coatings was performed based on the number of peptides found originating from proteins typical for specific protein additives. Furthermore, it was possible to identify the amino acid sequences that allow to determine the animal origin of the blood used. The non-protein components of all samples were identified by infrared spectroscopy, specifically by the attenuated total reflection and diffuse reflectance techniques. The quantitative analyses based on the change of the intensities of the measured spectra was also discussed. A special chapter is devoted to the identification of animal glue and to the...
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Nutrition during home hemodialysis treatment
Fialová, Petra ; Starnovská, Tamara (advisor) ; Dusílková, Nina Borisovna (referee)
The bachelor thesis "Nutrition of patients treated with home haemodialysis" deals with the analysis of diet and eating habits of patients treated with home haemodialysis. The aim of the thesis is to show that patients treated with home haemodialysis can follow a more moderate dietary restriction, thrive better and lead an active life. In the theoretical part I discuss the types of dialysis and their principles, complications of dialysis treatment, quality of life of dialysis patients and last but not least nutrition in different stages of kidney disease and dialysed patients. In the practical part, I based the results on structured questionnaires containing 20 questions and a detailed weekly menu. All 5 dialysis patients of the dialysis centre who are treated by home haemodialysis participated in the quantitative survey. The results of the survey showed that patients perceive a difference in their diet compared to conventional dialysis, with 60% of patients saying that they do not have to restrict themselves as much and 40% of patients even saying that they do not restrict themselves at all. Patients report these differences mainly in the amount of beverages, vegetables and fruits consumed. During the research, patients had normal laboratory results, felt better, experienced less fatigue and led a...
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Developing biomolecular interactions models for molecular simulations: Critical evaluation of force field parametrizations
Tempra, Carmelo ; Jungwirth, Pavel (advisor) ; Vácha, Robert (referee) ; Vega de las Heras, Carlos (referee)
Force field molecular dynamics methods are nowadays commonly used to study molecular interactions in many scientific fields. The accuracy of force fields has been improving over the years, allowing for a meaningful physical description of molecular phenomena. However, force fields have limitations. In this dissertation, I explored some of these limitations resulting from the parametrization strategy of force fields and the extent to which non-classical behavior, such as nuclear quantum effects, can be incorporated into classical force field molecular dynamics. In the first part, I investigated to what extent nuclear quantum effects can be accounted for within a classical force field for water. This allowed us to model the differences between bulk light vs. heavy water. The developed model was then used to describe solvent isotope effects on biomolecules, such as amino acids, proteins, and biomembranes, and to seek an explanation why heavy water (unlike light water) tastes sweet. In the second part, I pointed out the drawbacks of using certain training datasets in comparison to others when optimizing a force field, using aqueous calcium chloride as an example. In the third part, I demonstrated the importance of using an accurate water model during the optimization of force fields for phospholipids to adequately capture...
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Development of fortified gluten-free cereals for specific nutrition
Dohnalová, Radka ; Fialová, Lenka (referee) ; Márová, Ivana (advisor)
The bachelor thesis was focused on the development of enriched gluten-free cereals for special nutrition. The fortified cereal product included processed microbial biomass, either as a freeze-dried powder or as an extract, for which the phenolic content, antioxidant content and protein content were determined. The theoretical part includes findings from professional literature on gluten-free cereals, pseudocereals and their chemical composition. Subsequently, a review on microalgae and yeasts was prepared including a list of their metabolites. The literature search focused on green microalgae and carotenogenic yeasts. In the experimental part, the characterization of the selected pseudocereals and optimization of the prepared extracts of the selected microalgae and carotenogenic yeast were firstly carried out. The selected pseudocereals were amaranth, buckwheat and quinoa. The green microalgae strain Chlamydomonas reinhardtii and the carotenogenic yeast strain Cystofilobasidium macerans were selected for enrichment of the pseudocereals. Carbohydrates, proteins, fatty acids, -glucans, gluten, vitamins, phenolic substances, flavonoids and antioxidants were determined in the pseudocereals. Total fatty acids, lipophilic substances, phenolic substances and total antioxidant activity were detected in the microbial biomass. Finally, mixed cereal products were prepared from pseudocereal flour, microbial biomass and microbial extract. The cytotoxicity of the selected extracts was determined by MTT assay. It was found that the addition of algal biomass alone or algal extract to cereal flours resulted in a significant increase in protein. The yeast ethanol extract increased the phenolic and antioxidant content of the cereal products.
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Study of protein isolates digestibility
Čulíková, Andrea ; Juglová, Zuzana (referee) ; Pořízka, Jaromír (advisor)
Proteins are the structural components of all tissues of the human body. They are biopolymers that consist of amino acids. According to their origin, proteins are divided into plant and animal proteins. In the bachelor thesis, the properties of proteins isolated from wheat bran and commercial protein isolates from different plant and animal sources are discussed. Among the plant proteins, soy, almond, sunflower, chia and rice proteins were studied. Of the animal proteins, beef and whey proteins, among the most popular on the market, were studied. Specifically, the bachelor thesis deals with protein isolates, their composition, extraction methods by which protein isolates can be obtained from sources and subsequently their digestibility. The digestibility of protein isolates is one of the nutritional parameters that determines how well our body can utilize a given protein. In the experiments part, methods for the determination of protein content and digestibility in protein isolate samples were described. The Kjeldahl method was used to determine the protein content of the samples, and the results were then compared with the protein content indicated on the packaging. The enzymatic digestion process was simulated, the residue was subsequently lyophilized, and the digestibility of the protein isolate samples was studied gravimetrically. The results showed that whey protein was the best source of protein with respect to digestibility, with a PDCAAS value of 96.8% and a PDCAAS value of 0.94.
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Nutrition and supplementation in fitness and bodybuilding
Havlová, Ivona ; Kimmer, David (advisor) ; Šimková, Simona (referee)
Nutrition and Supplementation in Fitness and Bodybuilding Abstract The topic of this master's thesis is "Nutrition and Supplementation in Fitness and Bodybuilding". Physical fitness is getting more and more popular and becoming a global industry. Young people tend to prefer gyms to other types of physical activities. The advantage of fitness workouts is that there is no age limit, provided the person is healthy, as evidenced by exercising individuals who are 15 years old but also senior citizens over the age of 65. The empirical part of the paper was conducted using a qualitative method, gathering respondents through snowball sampling where participants identify others. As many as 28 respondents were divided into two groups: people doing conditioning and bodybuilding whose characteristics are explained in the Operationalisation chapter. All respondents did a semi-structured interview and provided a three-day diet plan. The goal was to map eating habits and the most common diet plans of people doing conditioning and bodybuilding, and to discover supplements they use the most frequently. The practical part of the thesis focused on the most frequent diet plans and supplements in fitness practitioners. The research findings indicate that the most common diet is high in protein and carbohydrates and low in fat,...
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The development of new isotope-coded cross-linkers for label free quantification of proteins
Procházková, Valérie ; Kukačka, Zdeněk (advisor) ; Vaňková, Pavla (referee)
Elucidating the structure of proteins in living organisms is an important step in describing their role. One method of structural biology is chemical cross-linking in combination with mass spectrometry, which is a commonly used tool for characterisation of the tertiary structure of proteins or protein-protein interactions. This method is based on the reaction of a cross-linking agent consisting of two reactive groups linked by an arm of a defined length to form a covalent bond. The main aim of the project was to verify whether chemical cross-linking using istopically labeled cross-linking agents can be used for quantification of two different structural states. The reagents used in this work were DSPU and an isotopically labeled form of DSPUx. Both the DSPU and DSPUx reagents contain a labile urea molecule in their structure, which is cleaved into characteristic fragments during collision-induced dissociation, allowing the identification of cross-linked peptides. Using top-down and bottom-up approaches, it was found on selected proteins (insulin, human carbonic anhydrase, and bovine serum albumin) that DSPU and DSPUx reagents interact with selected peptides and proteins and are suitable for quantification of structural changes. Subsequently, structural differences in the presence (holoform) and...
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Dynamic Processes in Porous Nanomaterials: Adsorption and Diffusion
Trachta, Michal ; Bludský, Ota (advisor) ; Heard, Christopher James (referee) ; Čičmanec, Pavel (referee)
Dynamical processes in porous nanomaterials (adsorption, diffusion, separation) play a significant role in many industrial applications. Modeling of these applications theoretically provides atomistic details of adsorption and diffusion phenomena - information that is often difficult to obtain using contemporary experimental methods. Theory may thus provide a deeper insight into these processes, e.g., explaining the mechanisms and assisting in the search for new materials suited for a specific application through material screening. In this work, the dynamical processes in porous materials were investigated by means of force field and ab initio modeling. On a set of relevant simulation data, two particular problems related to the modeling of such processes are thoroughly addressed: (i) the sensitivity of the results to material geometry, and (ii) the accuracy of the potential energy surface description. Regarding the structure description, the reliability of theoretically-predicted structures is still limited, despite the significant increase in computational power and algorithm development. The drawbacks of individual methods are identified; even state-of-the-art density functional theory approaches suffer from inaccuracies, e.g., due to an imbalance between the semi-local (GGA) functional and...
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Vliv alternativních zdrojů bílkovin v rybím krmivu na růst ryb a rostlin ve dvousmyčkovém akvaponickém systému
NIKL, Ondřej
The objective of this thesis was to evaluate the production efficiency of feeds which were designed with the intention of developing a special aquaponic feed. Further, to evaluate the content of nutrients in water, sludge and nutrient solutions and their influence on plant growth and finally suggest possible modifications to the feed composition. Aquaponic feed should respect nutritional requirements of fishes and plants, not put burden on the environment by not used nutrients and with regards to sustainability, use alternative sources of protein instead of marine fish meal. Because of that, three experimental isoprotein and isocaloric feeds were proposed. Feed A contained conventional ingredients including marine fish meal. Feed B used freshwater fish meal and exclusively plant proteins not regarding its origin. Feed C was made of sustainable and local sources of protein. The experiment was conducted in the aquaponic hall of Faculty of Fisheries and Protection of Waters, University of South Bohemia. As experimental material was used Nile tilapia (Oreochromis niloticus) and lettuce (Lactuca sativa). Feed B shown similar values of all production indicators as conventional feed A. Weight of fishes in system A after thirteen weeks increased by 143,68 % and in system B by 136,56 %. Weight of fishes in system B was at the end of experiment inconclusively lower by 4,08 %. Feed conversion ratio (FCR) of feed B (1,92?0,10) was inconclusively higher by 8,47 % against FCR of feed A (1,77?0,13). Average weight of aerial parts of plants growing in nutrient solution created from mineralized sludge gathered from system B, supplemented with missing nutrients reached value 221,8?33,9 g after four weeks. That was inconclusively more by 44,21 % than average weight of plants grown in special hydroponic solution and by 8,62 % more than weight of plants grown in enriched solution gathered from system A. Experimentally it was proven that in aquaponic feeds it is possible to substitute marine fish meal by alternative source of protein and using these feeds ensure an effective nutrition for fish and plants in a two-loop aquaponic system.
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