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Linear prediciton and cepstral synthesis of speech signal in the TTS system
Mekyska, Jiří ; Stejskal, Vojtěch (referee) ; Smékal, Zdeněk (advisor)
This work deals with a linear prediction and cepstral synthesis of speech signal in the TTS (Text-to-Speech) systems with the opportunity of modeling the prosody. The work contains a description of speech signal in acoustic and phonetic plane, the principle of speech production and the way we can figure the speech signal in time and frequency domain. Next, there is the TTS block structure mentioned, whereas each block has its own detailed description. In the work, the modeling of prosody using the three most important suprasegmental features (fundamental tone, continuation and speech intensity) is also described. At the end of this work, there is a design and realization of universal Czech TTS system which is based on the speech synthesis in frequency domain. This system is implemented in program MATLAB.
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Improving Markers for Augmented Reality
Páldy, Alexander ; Juránková, Markéta (referee) ; Herout, Adam (advisor)
Tato práce stručně popisuje moderní metody v rozšířené realitě a dále se zabývá generováním a detekcí Uniform Marker Fields. Hlavním cílem je zlepšovat markery známé jako Unifrom Checker-Board Marker Fields. Toho je dosaženo několika vylepšeními návrhu těchto markerů. Mezi hlavní patří využití širší barevné palety, zaměření na podobnost s vybraným obrázkem a změna tvaru modulů. Výsledný algoritmus byl navržen ke generování právě takových markerů. Výstupem jsou esteticky vypadající markery, které umožňují spolehlivou detekci i v případě výrazného překrytí nebo za špatných světelných podmínek.
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Biosensors based on modified nanoparticles
Mihajlović, Ana ; Majzlíková, Petra (referee) ; Hubálek, Jaromír (advisor)
This thesis deals with biosensors based on modified semiconductor quantum dots(AIIBIII). This work is divided into three main parts. The first deals with the theory necessary for the use of QDs in bioapplications, there are described methods of synthesis, application and bioconjugation of QDs . In the practical part, GSH-CdTe, CdTe-MPA and TGA-CdTe quantum dots were prepared by direct colloidal synthesis. These quantum dots were then modified by EDC in order to increase affinity to BSA (bovine serumalbumin). For the analysis of samples were used Infinite M200,Tecan and HORIBA Jobin Yvon FluoroLog detector Quanta . The third part is the summary of results and work achievements.
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Synthesis of spherical gold nanoparticles for biomedical applications
Gablech, Evelína ; Adam, Vojtěch (referee) ; Drbohlavová, Jana (advisor)
Tato práce se zabývá syntézou sférických zlatých nanočástic pro biomedicínské aplikace. Zlaté nanočástice byly syntetizovány ekologicky nezávadnými metodami, kterým je věnována i značná část rešerše. Cílem bylo nasyntetizovat stabilní koloidní zlaté nanočástice vhodné pro různé biomedicínké aplikace zejména, pro in vivo a in vitro zobrazovací metody, kterých přehled je take obsažen v teoretické části práce. Také byl proveden test cytotoxicity, jelikož částice mají být použitelné pro in vivo aplikace. Částice byly dale charakterizovány metodami SEM, DLS a UV-VIS.
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Solution of Continuous Systems by Evolutionary Computational Techniques
Lang, Stanislav ; Šeda, Miloš (referee) ; Olehla, Miroslav (referee) ; Matoušek, Radomil (advisor)
The thesis deals the issue of solution of continuous systems by evolutionary computational techniques. Evolutionary computing techniques fall into the field of softcomputing, an advanced metaheuristics optimization that is becoming more and more a method of solving complicated optimization problems with the gradual increase in computing performance of computers. The solution of continuous systems, or the synthesis of continuous control circuits, is one of the areas where these advanced algorithms find their application. When dealing with continuous systems we will focus on regulatory issues. Evolutionary computing can then become a tool not only for optimization of controller parameters but also to design its structure. Various algorithms (genetic algorithm, differential evolution, etc.) can be used to optimize the parameters of the controller, for the design of the controller structurewe usually encounter so called grammatical evolution. However, the use of grammatical evolution is not necessary if appropriate coding is used, as suggested in the presented thesis. The thesis presents a method of designing the structure and parameters of a general linear controller using the genetic algorithm. A general linear regulator is known also as so called polynomial controller, if we encounter the polynomial theory of control. The method of encoding the description of the general linear controller into the genetic chain is crucial, it determines a set of algorithms that are usable for optimization and influence the efficiency of the calculations. Described coding, effective EVT implementation, including multi-criteria optimization, is a key benefit of this work.
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Advanced ceramics for tissue engineering
Vojtíšek, Jan ; Koplík, Jan (referee) ; Bartoníčková, Eva (advisor)
This bachelor´s thesis deals issues of ceramics materials suitable for tissue engineering. An artificial substitute for human tissue can be divided to several generations. This include inert, bioactive and bioresobable materials. Resorbable substitutions are prepared from materials, which could substitute host tissue, for example hydroxyapatite. HA can be prepared in different ways (dry and wet way). To better application in medicine, ceramics foams prepared by in situ foaming or replication technique are using. Experimental part of this paper deals preparing of hydroxyapatite powder and process optimization. Samples was characterized by IR and Raman spectrometry, XRD analysis and particle size was studied by SEM. Ceramics foams was prepared by in situ foaming from Al2O3, HA and composites (in ratio 50 – 50, 75 – 25, 90 – 10). Porosity was evaluated by stereomicroscope, density and level of sintering was studied by SEM microscopy.
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Effect of environment on the photocatalytic properties of copper oxides
Šmatlo, Filip ; Vaněk, Jiří (referee) ; Vanýsek, Petr (advisor)
V této práci byl popsán princip fotokatalýzy. Práce se soustřeďuje na kovové oxidy, které mají široké využití ve fotokatalytických aplikacích. Bylo také popsáno různé použití fotokatalytických materiálů. Tato práce se zaměřuje zejména na využití fotokatalytických materiálů pro rozklad organických vodu znečisťujících látek. Popsaný fotokatalyzátor je oxid měďný, který má velmi dobré vlastnosti pro rozklad organických látek pomocí fotokatalýzy. Schopnost rozkladu organických látek oxidu měďného byla popsána na rozkladu organického barviva methylová oranž.
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Functionalization of gold nanoparticles for imaging
Jakubechová, Jana ; Pekárková, Jana (referee) ; Drbohlavová, Jana (advisor)
The diploma thesis deals with synthesis of gold nanoparticles and their surface functionalization suitable for in vitro imaging. In this view there are requirements for optical properties such as stability, monodispersity and no presence of cytotocxicity. In order to fulfill these demands the synthesis by Turkevich method with surface modification by glutathione and polytethylenglycol was performed. Analytical methods such as DLS, SEM and Zeta potential measurement were utilized to characterize the physical and chemical properties of synthesized gold nanoparticles. Finally, MTT assay was performed to evaluate toxicity of gold nanoparticles using HEK 293 cell line.
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Network Configuration Synthesis and Verification
Černeková, Alžbeta ; Veselý, Vladimír (referee) ; Ryšavý, Ondřej (advisor)
The subject of this master's thesis is to address the topic of network devices configuring. It resolves the problems related to simplifying certain parts of configuration while eliminating frequent errors or issues. It introduces a solution to setting of IP addresses on router's interfaces. Furthermore it presents the generation of configuration for particular dynamic routing scenarios and its application and methods for its verification on network devices. It also demonstrates the preparation of configuration of VPN tunnel between two devices. The conclusion of this thesis contains possibilities for further extensions or improvements.
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Preparation and characterization of poly-3-hydroxybutyrate copolymers
Kolomazník, Vít ; Kupka, Vojtěch (referee) ; Porubský, Tomáš (advisor)
This thesis evaluates the synthesis of copolymers based on poly-3-hydroxybutyrate (PHB). The goal is to create a material with the highest possible concetration of PHB with the smallest possible volume of crystal phase. PHB with lower molecular weight and hydroxyl end groups used for polyurethane synthesis was prepared by alcoholysis of PHB polymer with 1,4 butanediol. Polyethylene glycole (PEG) of various molar mass and ratio values was chosen in this thesis as a second reacting substance. Another substance used for copolymeration with PHB was the poly(butylene adipate-co-terephthalate) PBAT. Both variants used hexamethylenediisocyanate (HDI) as a non-toxic bonding agent. From these substances, a block copolymers comprised of soft and hard segments were formed. Hard segments are a result of PHB and it‘s crystalic structure. Soft segmets are comprised of PEG or PBAT macromolecules. The influence of soft segments of various length and molar ratio of reactants on overal polyurethane properties was evaluated. Prepared materials were charactrerized by thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and infrared spectroscopy (FTIR). Molecular weight was measured by gel permeation chromatography (GPC).
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