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Screening of extremozyme production of selected extremophilic PHA producers
Dyagilev, Dmitry ; Obruča, Stanislav (referee) ; Pernicová, Iva (advisor)
This bachelor thesis deals with the screening of the production of extracellular hydrolytic enzymes in thermophilic microorganisms of the genera Aneurinibacillus, Brevibacillus, Chelatococcus, Pseudomonas, Schlegelella, Tepidimonas and Caldimonas. The ability of selected enzymes, namely proteases, lipases, amylases, xylanases, cellulases and pectinases, was tested in the investigated microorganisms. Such testing made it possible to assess in which microorganisms the production of specific enzymes can be observed. Based on the results of the screening, it was found that Schlegelella aquatica LMG 23380, Tepidimonas fonticaldi LMG 26746 and the investigated microorganisms of the genus Chelatococcus did not show the ability to produce any of the tested enzymes extracellularly. In natural isolates of Brevibacillus borstelensis LK 99 and Aneurinibacillus thermoaerophilus LK 102, only the ability to produce lipolytic enzymes was detected. The isolate Brevibacillus borstelensis Bz acts as a universal producer of all selected extremozymes. Enzyme activity was determined for selected producers. The bacterium Brevibacillus borstelensis Bz proved the ability to produce all six selected hydrolytic enzymes and has the highest activity of lipases, xylanases, cellulases and pectinases from the tested microorganisms. The highest proteolytic activity was measured in Thermomonas hydrothermalis DSM 14834 when cultured on skimmed milk powder.
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Optimizing of explosing material screening and weighting
Plecháč, Jakub ; Kodytek, Jiří (referee) ; Videcká, Zdeňka (advisor)
The bachelor thesis deals with the optimization of screening and weighing of explosive materials in the company Explosia a.s. Based on a detailed analysis, improvements are proposed. The main proposal is to combine two types of screening and moving to a new object. The aim of the proposal is to improve hygienic and safety conditions in the workplace, to shorten the entire process and reduce costs.
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Screening of biosurfactant production in selected thermophilic bacteria
Řeháková, Veronika ; Nováčková, Ivana (referee) ; Obruča, Stanislav (advisor)
The subject of the bachelor thesis is the production of biosurfactants by selected thermophilic bacteria. The theoretical part contains a general characterization of thermophilic bacteria, describes the structures and classification of biosurfactants, characterizes their properties and uses. The final part of the theoretical part deals with the production of biosurfactants by thermophilic bacteria. The experimental part deals with the screening of biosurfactant production by selected thermophilic bacteria. Thermophilic bacteria were obtained from composts and activated sludges. Isolation is not a subject of the thesis. In total 39 bacterial isolates were cultivated and their supernatants were tested by screening methods – emulsification capacity assay and solubilization of crystalline anthracene. Based on the results of these methods, 14 most promising producers were selected. These bacterial strains were re-cultured to obtain a new set of supernatants. For more detailed screening of production by selected thermophilic bacteria, in addition to determining the activity of the emulsion and solubilization of crystalline anthracene, other methods were added: direct surface tension measurement – Du-Noüy-Ring, drop pouring method and oil spreading method. The results were evaluated and the measurements were repeated for a new set of supernatants to verify the reproducibility of the production. The results of all the tests were compared and then three most reliable thermophilic bacterial producers were determined.
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The Clock Drawing Test: Normative data for Czech elderly
Mazancová, Adéla ; Kulišťák, Petr (advisor) ; Vranka, Marek (referee)
The diploma thesis deals with the topic of limited normative data and its aim is to report the norms of the Clock Drawing Test (CDT) in a large sample of cognitively healthy Czech elderly (N = 501; aged 60 to 99). The CDT is a commonly used tool for screening of cognitive functions. We worked with the following version of test: clock drawing with pre- drawn circle and time setting 13.45 using three different scoring systems (Babins, Slater, Whitehead, & Chertkow, 2008; Cohen, Ricci, Kibby, & Edmonds, 2000; Shulman, Pushkar Gold, Cohen, & Zucchero, 1993). As a result, we found a significant effect of age and education, but non-significant effect of gender on CDT performance in old age. Therefore normative data was calculated considering those two demografical variables; the tables present means, standard deviations and t-scores based on defined subgroups. The results indicate that CDT performance of cognitively healthy elderly is negatively influenced by age, while directly proportional effect of education. Moreover it seems that the range of scores of the test grows with increasing age. Data collected in this thesis may be used for a more effective and standardized interpretation of CDT performance in old age in both clinical context and research. We also confirmed high inter-rater reliability...
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Problems of Autism Spectrum Disorders from the perspective of pediatricians
VLÁŠKOVÁ, Lenka
This bachelor thesis deals with the issue of autism spectrum disorders from the perspective of pediatricians. The aim of this thesis is to identify how general practitioners for children and adolescents in the South Bohemia region carry out screening for early detection of autism spectrum disorders in their practices and how they proceed in case of a positive result. The sub-objective was to find out what general practitioners for children and adolescents see as the biggest problem in the diagnostic process for these children. The thesis is divided into two parts - theoretical and practical. The first chapter of the theoretical part is devoted to individual autism spectrum disorders, the second chapter explains the so-called "triad" of problem areas, the third describes screening and diagnostic tools, and the last chapter focuses on the possibilities of care for individuals with autism spectrum disorders. The practical part is conducted through qualitative research and semi-structured interviews were used to collect data from pediatric and adolescent general practitioners. Furthermore, two case studies of children with autism spectrum disorder are presented in this part. Semi-structured interviews with parents and educational staff and a desk study were also used to collect data for the case studies. The data obtained show that general practitioners for children and adolescents perform screening for early detection of autism spectrum disorders during regular preventive examinations at 18 months of the child and repeat the screening again after six months in case of a positive result. Child and adolescent practitioners see the biggest problem in the diagnostic process as the lack of specialists to follow up their work.
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Human papillomavirus - prevention of cervical cancer and awareness of high school students about HPV viruses and their prevention
Dinga, Jana ; Thorovská, Alena (advisor) ; Zvírotský, Michal (referee)
This bachelor thesis is mainly focused on human papillomaviruses (HPV viruses), cervical cancer and primary prevention against HPV viruses. In the theoretical part, basic information about these viruses is described - from their history, epidemiology, risk factors that support the spread of viruses, to the ways of transmission of HPV infection and the division of HPV viruses according to their level of danger. The work also describes selected diseases caused by HPV viruses. Specifically, the work describes "less serious" genital warts (their epidemiology and incidence, mode of transmission and the way to treat or prevent this disease). A significant part of the work is devoted to cervical cancer as a disease caused by high-risk types of HPV viruses. The course of the disease and its individual phases are described in the thesis - from precancerous stages of cervical cancer to cervical cancer itself. A significant part of the work is devoted to the epidemiology of this disease and especially the possibilities of prevention against it (primary prevention, secondary prevention, etc.). In the research part, using the quantitative research method, I devised a questionnaire that was intended for high school students from the first to the fourth grade. Using the analysis of the results of the...
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Issues of lung CT screening for smokers
POKORNÁ, Miroslava
The communication deals with the evaluation of the benefits of the Pilot Programme "CT screening of smokers' lungs". The objectives and methodology include a theoretical study of the anatomy of the airways and the emerging issues of lung cancer, smoking dependence, low dose CT chest scanning techniques, National Radiological Standards and the issues of the target group of smokers and their involvement in investigation and treatment at specialist centres. The research was conducted through a questionnaire survey of 50 smokers (total 25 questions) and 41 physicians (total 18 questions). As a result, it was found that smokers' patients' awareness of the program is not sufficient, mainly due to their lack of interest in CT scanning and treatment options for the consequences of smoking and their unwillingness to actively cooperate with health professionals in their health care, including their refusal to give up the smoking habit. The outcome of the survey of physicians is good awareness and commitment to the implementation of the Pilot Programme, but they are limited in numbers and workload. The survey shows that the Pilot Programme is beneficial to the population. Therefore, it could prospectively be implemented by a larger number of health facilities in our country as part of a screening programme and thus contribute to improving the health status of the population. It was also found that the Hospital Jindřichův Hradec a.s. meets the requirements and could be included in the Pilot Programme.
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Clinico-pathological characteristics of mucin-producing colorectal carcinoma
Sojka, Ladislav ; Levý, Miroslav (advisor) ; Boublíková, Ludmila (referee) ; Klos, Dušan (referee)
Colorectal carcinoma (CRC) is one of the most common malignancies worldwide with persistently high mortality. As a consequence, high demands are placed on research into this disease. The aim of this study was to evaluate the epidemiology of colorectal carcinoma in the Czech Republic in comparison with worldwide data. We focused on the evaluation of the latest findings in screening and diagnosis, investigated prognostic factors, and also focused on the therapeutic options of CRC. Furthermore, mucin-producing carcinomas are described in detail and mucin itself was confirmed as a risk factor for the prognosis of CRC patients. The experimental part of the study focused on gene mucin MUC13 and the MUC13 - miR-4647 axis, which is associated with adverse prognosis in CRC patients. Samples of CRC and samples of adjacent non-malignant mucosa tissue were used. The samples were collected from patients who underwent surgery at the General University Hospital in Prague, at the Thomayer University Hospital in Prague and at the University Hospital in Pilsen (Czech Republic). The collection of samples took place between 2011 and 2015. The total of 187 samples were collected from patients with sporadic colorectal cancer. The subjects of the study all provided information on their lifestyle habits, body mass index...
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