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Development of a procedure for the analysis of the T-cell response against SARS-CoV-2 virus
Šmahel, Michal ; Pavliš, Oto ; Beran, Ondřej ; Bohoněk, Miloš ; Tachezy, Ruth ; Holub, Michal ; Poláková, Ingrid ; Saláková, Martina ; Schreiberová, Lucie
In 2020, the SARS-CoV-2 coronavirus caused a pandemic of covid-19 disease. To detect the cellular response induced by infection with this virus, a highly sensitive ELISPOT assay has been developed that detects specific interferon gamma-producing T lymphocytes after stimulation with synthetic peptides derived from viral antigens. This assay allows the detection of memory T cells directed against the viral spike (S) protein used for vaccination as well as against other viral proteins. This method can be applied to the analysis of the immune response in individuals after covid-19 and SARS-CoV-2 vaccination and may thus be used to determine the immune preparedness of emergency workers.
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Design of the linear axis for the centroscope measuring device
Kopec, Adam ; Pavlík, Jan (referee) ; Holub, Michal (advisor)
The final thesis deals with the creation of a structural design of a linear motion axis for the measuring device centroskop. A search in the field of offered centroscopes was carried out and the following work was based on the specified parameters. In the system analysis, the use of the given types of design nodes was considered. The basic concept of the machine structure was finally determined by selecting a suitable variant, here multicriteria analysis was used. This was followed by a detailed design of the individual machine parts, involving engineering calculations and FEA analysis. The centroscope axis was built digitally in Autodesk Inventor. Subsequently, an assembly drawing with parts list and some additional drawings of major components were created. Beyond the brief, a basic concept of the axis support frame was also created.
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Application of Industry 4.0 elements in production lines
Knápek, Tomáš ; Klíč, Daniel (referee) ; Holub, Michal (advisor)
This bachelor thesis deals with the possibilities of using Industry 4.0 elements in production lines in order to improve the characteristics of these lines and reduce the workload of employees. In addition to an introductory presentation of the concepts related to the topic, the reader can read about the current state of a particular line and proposals for its modification using Industry 4.0. One of these proposals is then elaborated in more detail in the following section, including risk analysis and technical calculations. The main objective of the proposal is to increase the performance of this line as significantly as possible.
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Stability measuring straightness errors in long-term tests
Moravčík, Patrik ; Vetiška, Jan (referee) ; Holub, Michal (advisor)
This thesis is focused on the design and the verification of the automatic correction of the straightness measurement error. Experimentally verified are mechanic gadgets for soft rotation. Several prototypes were created. All of them were tested and evaluated. The whole experiment is evaluated in the conclusion and recommendation for real application was written. In patent research were not any patents relevant to this thesis found, thus we apply our own.
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Application of Industry 4.0 elements in production lines
Knápek, Tomáš ; Klíč, Daniel (referee) ; Holub, Michal (advisor)
This bachelor thesis deals with the possibilities of using Industry 4.0 elements in production lines in order to improve the characteristics of these lines and reduce the workload of employees. In addition to an introductory presentation of the concepts related to the topic, the reader can read about the current state of a particular line and proposals for its modification using Industry 4.0. One of these proposals is then elaborated in more detail in the following section, including risk analysis and technical calculations. The main objective of the proposal is to increase the performance of this line as significantly as possible.
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Design of headstock of the deburring machine
Tatíček, Jiří ; Holub, Michal (referee) ; Omes, Jiří (advisor)
The aim of this thesis is design of deburring machine, focusing on the headstock, capable of aligning the front side and chamfering at the round bars. At the beginning is a short research design nodes, used in the construction of machine tools. As part of the work was done and the measurement of cutting forces to determine the load while standing. Work also includes a series of calculations to justify the use of selected components. The outcome of the work is also a 3D model.
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Design methodology for the selection of basic components of linear machine tool axes
Král, Stanislav ; Dosedla, Michal (referee) ; Holub, Michal (advisor)
The aim of the thesis is based on specified parameters positioning units, which include stroke (1m, 2m, 4m), load (5kg, 50kg, 500kg), you place the position accuracy (0.04 mm) and dynamic (maximum attainable speed 1.5 m / s), proposals to build a ball screw, linear motor, a toothed belt and toothed comb. For selected variants of positioning units to design a 3D model of positioning units. The proposed variations to compare and define their advantages / disadvantages, techno-economic comparison, the limit values of individual designs, etc. The work includes a search drive in positioning units, design units with toothed belt, a toothed comb, ball screw linear actuator, a calculated message of individual proposals then 3D design options for the ball screw and linear motor including drawings and assembly drawings.
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Design of elongated right angle milling head for horizontal boring machine
Svoboda, Ondřej ; Tůma, Jiří (referee) ; Holub, Michal (advisor)
This thesis deals with design of right-angled elongated milling head for horizontal boring machine of FERMAT production. The thesis includes a detailed research of milling heads, a description of the head´s basic components and certain commercially available head´s comparison. Design part is describe components description and the design. Calculations are focused on determinating of forces generated during machining, setting of loading spectrum and the power load calculating individual gears during machining. In the next part is calculation of the reactions in the spindle bearings included, the equivalent load and durability of spindle bearings, spindle deformation and spindle bearings optimum distance. The thesis also includes spindle strength calculation, horizontal and vertical shafts analysis, gears design with helical teeth and gear strength. The thesis describes the design of extended milling head and particular design nodes which is supplemented by the 3D model. In this thesis is also included a drawing of assembly and components.
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Design apparatus for measuring geometric accuracy of machine tools
Holcman, David ; Marek, Tomáš (referee) ; Holub, Michal (advisor)
This bachelor’s work deals with modern methods of geometric accuracy measurement of machine tools, modal analysis and definition of demands on used agents during the measurement process. A part of this work is dedicated to description of numerical finite element method, which is the cornerstone of ascertaining the natural frequencies by a software program. In the closing part the appropriate adjustments of minimisation of influence on measurement are suggested.
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