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Encapsulation of active substances into nanofibers and possibilities of their application
Procházková, Lucie ; Pernicová, Iva (referee) ; Skoumalová, Petra (advisor)
The master thesis was based on the optimization of the production of nanofiber covers and to gaine the product for subsequent functional use. The production of nanofiber covers was made by electrospinning and forcespinning from selected materials. Polyhydroxybutyrate, gelatin, chitosan and alginate were used as starting materials. After successful optimization, these materials were enriched with active ingredients ampicillin and ibuprofen for the functionalized use of covers for more effective wound healing. The theoretical part was focused on the issue of skin, healing processes, types of wounds and nanofibers, the characterization of selected starting materials for the formation of nanofibers was also mentioned. The practical part was based on the lengthy optimization of the preparation of fiber covers and later enriched with active ingredients. Furthermore, combined covers made of different materials with contents of both active ingredients were designed. This was followed by the characterization of all prepared covers from the point of view of stability in the short and long term. The gradual release of active ingredients was determined spectrophotometrically and by hifh performance liquid chromatography. It was also important to determine the antimicrobial activity of selected active substances. At the end of all testing, combined coatings containing both active ingredients were used for safety testing with human keratinocyte cells (HaCaT). Safety testing was based on determining the viability of human cells using the MTT test, to verify the LDH test. A scratch test was also performed, a wound healing test after the application of devised combined covers.
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Determination of antimicrobial activity of berry fruit extracts
Procházková, Lucie ; Veselá, Mária (referee) ; Vítová, Eva (advisor)
The goal of this bachelor´s thesis was the determination of effect of model sample of chokeberry extract (Aronia melanocarpa) on selected types of microorganisms (bacteria Bacillus cereus and Escherichia coli, yeast Candida glabrata). Two commercially available samples of natural extracts from raspberry and cocoa were selected and analysed for comparison. Theoretical part is focused on characteristic, composition and properties of mentioned plant materials, there are also listed their uses in pharmacy, medicine and cosmetics and possible methods of determination of antimicrobiological activity. Analysed commercially available samples of raspberries and cocoa don´t show any antimicrobial activity to selected microorganisms with the exception of 100% raspberry extract on Candida glabrata. There was growth promotion of colonies in the surroundings of the extract in other microorganisms. Ethanol extract of chokeberry fruits showed growing antimicrobial activity with increasing concentration of the extract. The strongest potential was observed in yeast Candida Glabrata and in bacteria Bacillus cereus. The bacteria Escherichia coli was completely resistant.
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Concepts and legal consequences of an excessive use of self-defence and necessity
Procházková, Lucie ; Vokoun, Rudolf (advisor) ; Pelc, Vladimír (referee)
The concept and legal consequences of excessive self-defense and necessity Abstract The presented master's thesis deals with the criminal law institutes of self-defense and necessity, especially their legal conditions and legal consequences associated with their violation. In the system of Czech criminal law, both institutes hold a place among the explicitly regulated circumstances excluding criminal liability. They are significant institutions that enable anyone to protect their legitimate interests or the legitimate interests of others, the state, or society as a whole when these interests are threatened by a certain danger, and the competent authorities are unable to ensure their proper protection. The fact that an act was committed in a situation of necessity or self-defense excludes the unlawfulness of such conduct and, consequently, the criminal punishment associated with it. However, for such conduct to be considered permissible and beneficial for society, it is essential that the conditions and limits stipulated by the criminal code for actions in necessity and self-defense are met. The aim of this work is to comprehensively analyze these individual conditions and limits, and subsequently define the concept and different types of excess in necessity and self-defense. The thesis also thoroughly...
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The impact of development aid on democracy and human rights in Haiti in the 21st century.
Procházková, Lucie ; Krausz Hladká, Malvína (advisor) ; Martinková, Viera (referee)
The Democratic Republic of Haiti occupies the western part of the island of Hispaniola, which it shares with the Dominican Republic. Haiti is one of the poorest countries in the world, and despite the significant amount of development aid that flows into the country each year, the country remains in a critical state. The lack of integrity of the local government, the strengthening of criminal gangs, and the unsustainable state of the economy are just a few examples of the factors that influence this whole process. The bachelor thesis, therefore, deals with the power of development in Haiti. It describes individual's pacts and requirements of aid, the motivation of donors, or internal and external aspects that influence the applicability of aid in the country. It also looks at there as one for the failure of aid, which prevents the establishment of sustainable development in the country. In my work, I try to come up with possible solutions or steps that could move the situation t establishment of sustainable development in the country.
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The phenomenon of the Peace Race in the context of time
Procházková, Lucie ; Horčička, Václav (advisor) ; Pešta, Mikuláš (referee)
This diploma thesis deals with the cycling stage Peace Race. The aim is to demonstrate the link between politics and sport. Specifically, the abuse of sport to promote political views. Therefore, period materials, such as spectator letters, newspaper articles or reports between race organizers, will be analyzed and the consequences drawn. The work deals with specific years that directly influenced the course and organization of the Peace Race. The main emphasis is on the first years of the race and then on the 80s of the 20th century, when there was an accident at the Chernobyl nuclear power plant, which had a great impact on the race. Key words: Cycling, Czechoslovakia, Peace Race
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Encapsulation of active substances into nanofibers and possibilities of their application
Procházková, Lucie ; Pernicová, Iva (referee) ; Skoumalová, Petra (advisor)
The master thesis was based on the optimization of the production of nanofiber covers and to gaine the product for subsequent functional use. The production of nanofiber covers was made by electrospinning and forcespinning from selected materials. Polyhydroxybutyrate, gelatin, chitosan and alginate were used as starting materials. After successful optimization, these materials were enriched with active ingredients ampicillin and ibuprofen for the functionalized use of covers for more effective wound healing. The theoretical part was focused on the issue of skin, healing processes, types of wounds and nanofibers, the characterization of selected starting materials for the formation of nanofibers was also mentioned. The practical part was based on the lengthy optimization of the preparation of fiber covers and later enriched with active ingredients. Furthermore, combined covers made of different materials with contents of both active ingredients were designed. This was followed by the characterization of all prepared covers from the point of view of stability in the short and long term. The gradual release of active ingredients was determined spectrophotometrically and by hifh performance liquid chromatography. It was also important to determine the antimicrobial activity of selected active substances. At the end of all testing, combined coatings containing both active ingredients were used for safety testing with human keratinocyte cells (HaCaT). Safety testing was based on determining the viability of human cells using the MTT test, to verify the LDH test. A scratch test was also performed, a wound healing test after the application of devised combined covers.
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Representation of the sixties of 20th century in literary autobiography
Procházková, Lucie ; Kubíček, Tomáš (advisor) ; Mocná, Dagmar (referee)
This work is an attempt to gain a deeper understanding of the 1960s through an in-depth analysis of selected literary autobiographies. The first section is devoted to the theoretical foundation for exploring the chosen four texts of autobiographical literature. It is based on the analysis and subsequent comparison diaries of Ivan Diviš and Pavel Juráček and the memory of Vaclav Černý and Ota Filip. Additionally, this paper examines how these writers created an image of the 1960s and early 1970s.mFurther exploration includes narrative elements, for example, by what means texts are constructed, character space time, composition, and relationship to the audience. Moreover, the meaning of writing autobiographical literature, specifically the selected works, will be explored. Lastly, these major themes set the stage for the comprehensive analysis of the authors' works, revealing the creative design, history, society and culture of their time.
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Deserted mediavel village "Mořina" at Bšštín (Příbram)
Korbová Procházková, Lucie ; Klír, Tomáš (advisor) ; Smetánka, Zdeněk (referee)
This bachelor thesis concerns deserted medieval village Roudnicka in location "Morina". It is archeological site that was known and researched already in 19th century, but wrong interpreted as an slavic barrow field. It has been the best-researched middle age village in Bohemia until circa 1965. The critical revision of results of previous survey was made and the geodeticaltopographical survey of village relicts was carried out. It was possible to obtain detailed drawing to restore residental form and adumbrate appearance of yards of particular estates.
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Effect of latent toxoplasmosis on the thyroid function during pregnancy
Procházková, Lucie ; Kaňková, Šárka (advisor) ; Springer, Drahomíra (referee)
While several past studies dealt with the effects of latent toxoplasmosis on the function of the thyroid gland and on the pregnancy separately, the former one with varied results, this work combines the two areas together with goals to determine whether the latent toxoplasmosis affects levels of antibodies against thyroid peroxidase (TPO) in the 1st trimester of pregnancy, to analyze the effect of toxoplasmosis on the levels of the thyrotrophic hormone and to learn whether the interactions between latent toxoplasmosis and autoimmune thyropathies affect conception, foetal development, length of pregnancy and mode of delivery in childbirth. The work follows on the recent years' discoveries of our laboratory that showed the latent toxoplasmosis to affect both the course of pregnancy and the prenatal development of a child. It also takes up directly on a screening of autoimmune thyropathies in pregnant women conducted in years 2006 to 2009 in General University Hospital. The retrospective study comprised of 1434 pregnant women who underwent serological examination as a part of the 1st trimester screening and whose serum was subsequently examined for toxoplasmosis. The data set obtained from medical databases contained results of serological screening of the mothers, ultrasound examination of the...
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