National Repository of Grey Literature 129 records found  1 - 10nextend  jump to record: Search took 0.00 seconds. 
Epigenetic Regulation of Adhesive Molecules in High-grade Serous Ovarian Carcinoma
Baranová, Ivana ; Palička, Vladimír (advisor) ; Matoušková, Petra (referee) ; Friedecký, David (referee)
5 Summary Title: Epigenetic Regulation of Adhesive Molecules in High-grade Serous Ovarian Carcinoma The lack of effective biomarkers for screening and early detection of ovarian cancer is currently considered as one of the most pressing problems in oncogynecology. Because epigenetic alterations occur early in the cancer development, they provide great potential to serve as such biomarkers. Epigenetic mechanisms have been implicated also in regulation of adhesion molecules that play a major role in cancer progression. The main aim of this study was to investigate the methylation pattern of selected cadherin and catenin genes in ovarian cancer tissue by comparison with control tissue. The study group consisted of 68 patients with high-grade serous ovarian cancer (HGSOC) and 46 control patients. To determine the sites with the most significant methylation in selected genes next-generation sequencing was employed. For further confirmation of detected methylation of selected regions, methylation-sensitive high-resolution melting analysis and real-time methylation-specific polymerase chain reaction were used. In attempt to design potential biomarker panel for future screening of HGSOC as the secondary aim of our study, cadherins were evaluated together with transcription factors from our previous study....
Effect of imatinib on selected mice microRNAs
Heglasová, Silvia ; Matoušková, Petra (advisor) ; Skarková, Veronika (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Silvia Heglasová Supervisor: Doc. Ing. Petra Matoušková, Ph.D. Title of diploma thesis: Effect of imatinib on selected mice microRNAs Imatinib (IMB) belongs to low molecular weight tyrosine kinase inhibitors (TKI). One of the undesirable effects of IMB is cardiotoxicity. Early diagnosis of heart damage is important for improving human health. MicroRNAs (miRNAs) are intensively studied as potential early biomarkers of cardiac damage. They are not only intracellular but also circulating freely in the bloodstream. Plasma miRNAs are stable, resistant to repeated freezing and methods of their detection are very sensitive. In my diploma thesis I have studied changes in the expression of miRNAs in plasma in the IMB induced heart damage. I have analyzed circulating plasma miRNAs and also miRNA levels in mice hearts. Selected miRNAs were measured by quantitative real-time PCR (qPCR). Plasma Troponin T levels were also measured as classical biomarker of cardiotoxicity. Troponin T levels in the IMB-treated plasma were very variable. This fact points to different responses of individuals to IMB. Changes in expression of observed miRNA in plasma affected by IMB were not significant. Statistically...
Effect of doxorubicin on selected mice microRNAs
Dugasová, Lucia ; Matoušková, Petra (advisor) ; Jansová, Hana (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Lucia Dugasová Supervisor: doc. Ing. Petra Matošková Ph.D. Title of diploma thesis: Effect of doxorubicin on selected mice microRNAs Doxorubicin (DOX) belongs to the anthracycline antibiotics used by patients suffering from cancer. Its undesirable effect is cardiotoxicity. Prognostic of cardiotoxicity depends on its early detection, and therefore many studies examine the properties of microRNAs that could be appropriate biomarkers for heart failure in the future. MicroRNAs are involved in almost all processes in the human body, in development, differentiation, proliferation and apoptosis. They are also located in the extracellular space, they are stable and have many other positive properties. My diploma thesis is focused on monitoring changes in microRNA levels that can occur in heart damage by doxorubicin. The cardiac tissues and plasma of mice treated with doxorubicin were used as samples and compared with control samples. Samples were processed and evaluated by real-time quantitative PCR (qPCR). A statistically significant increase in expression in heart tissue occurred only in miR-34a and decrease in miR-205 level. Plasma mice samples showed a statistically significant increase in...
Effect of selected tyrosine kinase inhibitors on the activity of human carbonyl reducing enzymes
Tomanová, Alžbeta ; Novotná, Eva (advisor) ; Matoušková, Petra (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Alžbeta Tomanová Supervisor: RNDr. Eva Novotná, Ph.D. Title of diploma thesis: Effect of selected tyrosine kinase inhibitors on the activity of human carbonyl reducing enzymes Key words: tyrosin kinases, screening, carbonyl, daunorubicin, inhibition Tyrosine kinases are a subclass of protein kinases, catalysing the transfer of ATP phosphate residue to a protein, thereby playing an important role in cellular signaling. Abnormal tyrosine kinase activity is present in various malignancies. In certain cases, inhibition of their function can prevent tumor cell proliferation and eventually induce apoptosis. At the same time, some tyrosine kinase inhibitors have demonstrated the ability to inhibit efflux transporters, which are often involved in the development of resistance to anticancer treatment. In this diploma thesis, the inhibitory effect of imatinib, nilotinib, dasatinib and acalabrutinib (tyrosine kinase inhibitors) has been studied on carbonyl reducing enzymes, whose overexpression by tumor cells may lead to resistance to chemotherapy. In particular, in the case of anthracyclines, the reduction of carbonyl group on C-13 results in not only lower cytotoxic activity, but also increased...
Study of expression of glutathione peroxidases in mice with induced obesity
Zemanová, Kateřina ; Matoušková, Petra (advisor) ; Skálová, Lenka (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Kateřina Zemanová Supervisor: Ing. Petra Matoušková, PhD. Title of thesis: Study of expression of glutathione peroxidases in mice with induced obesity Obesity is serious worldwide health issue. The most common reason for developing obesity is unbalanced intake and energy expenditure. It is connected to a higher amount of reactive oxygen species (ROS) in organism, which increases the risk of developing health issues related to obesity. Between these issues are belonging mainly cardiovascular diseases, metabolic syndrome or type two diabetes mellitus. The goals of many studies focusing on obesity are identification of new biomarkers, searching for target structures for obesity treatment and potentially developing antiobesity drugs. Glutathione peroxidases (GPxs) are enzymes with antioxidation activity. Currently, there are eight GPxs described. They can reduce the amount of reactive oxygen species (ROS) produced in our body. The GPxs can cause damage to the surrounding tissues in case of their accumulation and they are related to many diseases. Based on these findings, efforts have been made to discover how the expression of GPxs genes is regulated and how they are connected to microRNA...
Biotranformation of fenbendazole in soya (Glycine max)
Martínková, Lenka ; Raisová Stuchlíková, Lucie (advisor) ; Matoušková, Petra (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Lenka Martínková Supervisor: RNDr. Lucie Raisová Stuchlíková, Ph.D. Title of diploma thesis: Biotransformation of fenbedazole in soya (Glycine max) Veterinary drugs are used in large amounts in modern husbandry for treatment and prevention of diseases in animals. Anthelmintics administered to animals enter into environment primarily through its excretion in faeces or urine. Following excretion, drugs may persist in the environment and impact non-target organisms. Plants are able to uptake xenobiotics, including drugs, and detoxify them via biotransformation. However, only drug biotransformation into non-toxic and stable metabolites and their consequent accumulation in plants represent drug detoxification. For that reasons, knowledge of biotransformation pathways of drug in plants is very important. Soybean plants, eventually seeds, are further used in agriculture as feed for cattle and absorbed anthelmintics including their metabolites can enter the food chain. The results showed that fenbendazole entered plant and enzymatic systems of plant were able to biotransform fenbendazole via several reactions. We found differences in the metabolites between the roots, leaves, seeds and pods of soybean.
Characterization of intermediates and waste products of some fermentation productions
Kilian, David ; Matoušková, Petra (referee) ; Márová, Ivana (advisor)
This bachcelor thesis was aimed on characterisation of intermediate and waste products of some fermentation productions. The theoretical part of this thesis is aiming on characterization of raw material and its fermentation, process of distillation, waste product and introduction of main active components of all these intermediates. In the experimental part were characterized intermediates and products regarding the contents of sugar, ethanol and polyphenol. All these extracts contain particular levels of polyphenol and sugar corresponding to individual phases of fruit distillates production. The chemical characteristics of extracts from different phases of fruit distillates production were compared. Parameters as total phenolics, flavonoids, glucose, fructose and ethanol were evaluated. Subsequently the changes between individual phases were described. In conclusion, a solution of further use of waste products formed during fruit fermentation process was suggested.
Development of new type of energy drinks based on coffee with active substances
Dudrová, Markéta ; Matoušková, Petra (referee) ; Márová, Ivana (advisor)
This bachelor thesis deals with development and preparation of new energy drinks with active plant component in free and encapsulated form. The theoretical part is focused on characterization of the energy drinks, their components and properties. The experimental part deals with the preparation of plant extracts, their characterization and subsequent encapsulation into liposome particles. All particles were determined for size, colloidal stability, long-term stability, antioxidant activity and encapsulation efficiency. Energy drinks based on various types of coffee were prepared and enriched with free or encapsulated guarana, goji, matcha or cocoa extract. In prepared energy drinks the content of caffeine, sugar, phenolics and antioxidant aktivity was monitored. Finally, selected energy drinks were subjected to sensory analysis, where appearance, color, smell and taste were evaluated.
Non-traditional sources of proteins and their potential applications
Kubisová, Petra ; Fialová, Lenka (referee) ; Matoušková, Petra (advisor)
This bachelor thesis is focused on choosing the non-traditional sources of proteins, their characterization and subsequently its potential applications in diet of sportsmen. The theoretical part is focused on specification of importance of proteins in nutrition, description of different available protein supplements for sportsmen and essential characterization of chosen samples. The practical part of this bachelor thesis is focused on analysis of proteins, sacharides and lipids in samples. Nutritional components were analysed by different methods such as spectrophotometric methods, electrophoresis, gas chromatography etc.

National Repository of Grey Literature : 129 records found   1 - 10nextend  jump to record:
See also: similar author names
4 Matoušková, Pavlína
1 Matoušková, Petra Bc.
Interested in being notified about new results for this query?
Subscribe to the RSS feed.