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Building and use of smaller computer networks
Kyzivát, Marek ; Pinkas, Otakar (advisor) ; Šmejkal, Ivo (referee)
The thesis describes the initial state of the computer network Basic Schools and Kindergartens Ohradní (ZŠMŠ Ohradní) in Prague 4 and then its reconstruction, which I largely contributed. ZŠMŠ Ohradní consists of four pavilions and from one building on the other side of the street. The aim of this study is to assess the initial state of the network and point out the shortcomings that we discovered. We found deficiencies in the operation stress tests, measurements and data cabling overall analysis. Original networks connectivity was 100Mb/s. Together with a colleague, we are network administrators. Furthermore, I am going to introduce a new form of network, which has identified deficiencies improved, by using on newer technologies. I will describe its structure, address allocation, active and passive components, and operated services. New network is capable of 1Gb/s, and its spine is going to be 10Gb/s soon. Benefits of this reconstruction is faster, more stable and more modern network that will facilitate the work of employees of ZŠMŠ Ohradní. To these improvements, I have mainly contributed by measurements. I participated in the design and implementation of new form of data network. I collaborated with fellow Poc to the configuration of active elements and network services in reconstructed network.
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Design of marketing strategy in the company Imperium Finance
Petrovič, Marko ; Čermák, Radim (advisor) ; Sova, Martin (referee)
Internet marketing is currently one of the most important components of marketing. However, Internet marketing as a whole is much more important than mere webdesign, mere search engine optimization (SEO) or a simple PPC advertising. Most companies have recently made it throught with a website and that was all their presentation on the Internet. However, the internet is one of the fastest growing technology today and the number of its users is growing every year. Constantly there are new technologies that allow us to have a better user experience and also bring us new ways of presentation and promotion. Most of the companies make a mistake early on and underestimate the preparation, don't know their goals, customers or competitors. Internet marketing is the process and the contents of this process is the recognition, analysis, design, implementation, and ultimately verify that you achieve the goals of the organization. In the theoretical part it is defined internet marketing and its advantages over offline marketing. Briefly sum up the history and major milestones of Internet marketing that shaped its present form. Further, it's described current trends in Internet marketing and detail expands upon the selected Internet marketing tools and tools for evaluating the success of campaigns. The practical part deals with the proposal of marketing strategy in the company Imperium Finance. The main objective is to analyze the requirements of the company Imperium Finance, from analysis to draw specific conclusions and devise a marketing strategy that will support the vision and strategy of the company in the following period. All steps were carried out within the company Imperium Finance on real data from active clients and tested in action. The result is a proposal of a marketing strategy that will drive Imperium Finance, and a marketing plan for the company. The biggest benefit of this work is so real use theoretical knowledge in practice.
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Effect of low doses of herbicide paraquat on antioxidant defense in Drosophila
SÁBOVÁ, Michala
Disruption of cell equilibrium between production of free radicals and antioxidant defence is named oxidative stress. The main component of antioxidant mechanism is activity of antioxidant enzymes, which include superoxide dismutase (SOD) and catalase. In this study we evaluated the effect of herbicide paraquat on response of SOD and catalase, their transcription level and locomotion activity in Drosophila melanogaster. We exposed the flies to a wide range of paraquat concentration. Our results revealed that transcript and enzymatic levels of both SOD and catalase have a similar biphasic dose response with the peaks at 2,5 ?M paraquat concentration, resembling hormetic effect. Then, males were more sensitive than females. However, females had an increased locomotion activity. We found that paraquat susceptibility is increased in males and mating flies. Therefore, this study supports hypothesis of stress sensitivity elevation as a physiological cost of reproduction.
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PM2.5 Chemical Composition at the Rural Background Site Košetice.
Schwarz, Jaroslav ; Cusack, Michael ; Karban, Jindřich ; Chalupníčková, E. ; Havránek, Vladimír ; Smolík, Jiří ; Ždímal, Vladimír
Measurements of atmospheric aerosols at rural background areas are important because they are not influenced by the urban emissions, and are therefore better suited to investigate the influence of long-range transport and trends in concentrations of individual pollutants. Summary of the measurements of total concentration and the concentration of PM10 and PM2.5 components was carried out in the works Putaud et al. (2004, 2010). Although the later work included the measurement of more than 60 stations across Europe, the post-communist countries contained data on PM2.5 from only four locations (Prague, Debrecen, Hungary, and two rural locations). Although Košetice place where this study was made, is also discussed there, it was for only PM10 and total carbon (TC). Therefore, the aim of this study is to fill this information gap, and provide additional information regarding the PM2.5 fraction and composition at the Central European background station Košetice.
Fulltext: content.csg -  PDF
Plný tet: SKMBT_C22016110313110 - PDF
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Treatment of Flue Gas from Waste Incineration by Sodium and Calcium Based Sorbents.
Zach, Boleslav ; Pohořelý, Michael ; Šyc, Michal ; Svoboda, Karel ; Punčochář, Miroslav
This paper is focused on properties of sodium and calcium based sorbents in relation to simultaneous dry flue gas treatment from SO2, HCl, NOx, solid particles, and polychlorinated dibenzodioxins and dibenzofurans (PCDD/F). The most important differences between the two sorbent types are: the effect of temperature and moisture of flue gas on the sorption process, reactivity towards individual acid components in the flue gas (thermodynamic equilibrium and kinetics), and solubility of air pollution control residues.
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Puppies and young dogs nutritions
Šabatová, Kateřina ; Hučko, Boris (advisor) ; Plachý, Vladimír (referee)
Puppies and young dogs nutritions Summary In this work I have attempted to summarize the most important aspects in nutrition of puppies and dogs in growth. The introduction describes the digestive tract and also the changes that have occurred due to domestication. Next is explained the importance of nutrition and water in nutritional requirements of a dog. Important are needs of nutrients and energy that are necessary to maintain the provision of the necessary vital functions. The basic components of food are proteins, saccharides, lipids, minerals and vitamins. I explain the necessary proportions, the amounts and the possible consequences of their lack in food. I focus on essential fatty acids, which in recent years are subject to frequent explorations and their pros and cons are not yet fully understood. I also mention ballast and significant specific active substances. Nutrition of pregnant and lactating females is often neglected, but for puppies it is a very crucial period. The influence of nutrition of pregnant females to the size and weight of newborn puppies has been proved. Further I deal with nutrition of puppies immediately after birth, I explain the composition of colostrum and its effect on the immunity system of the puppy. I also compare ratios of nutrients in the colostrum and milk. Further, I deal with the feeding of the individual at the time of weaning and in different growth periods. There are many possibilities of feeding puppies from after weaning until adulthood described in books. With the new knowledge about nutrition the ways of feeding our dogs have inevitably changed , but also the dogs' nutritional requirements have changed due to the conditions where and how they live and what their nutrient needs are according to their sport, breeding or other use. Currently, there is a possibility of a commercially produced complete nutritional diet or home-prepared fresh food - cooked or raw- barf. In the last chapter, I summarized possible aspects of malnutrition and its potential consequences. I described the most common problems associated with inadequate nutrition of growing dogs such as obesity as well as malnutrition, which also adversely affects the individual. The influence of proper nutrition of the puppy on its growth is clear. If we neglect the nutrition of the dog in the first two years of its life, the consequences are often irreversible.
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Microbiota of the digestive tract of bees and related insect and the influencing facftors
Hroncová, Zuzana ; Havlík, Jaroslav (advisor) ; Kalous, Lukáš (referee)
This thesis consists of six research chapters, out of which, five have already been published in research journals, 1 is in the process of submission and the last chapter presents original unpublished confidential data in the process of manuscript preparation.
The research presented in this thesis concerns the complex mechanisms of bees and wasps immune system focused on microbiota as component of immunity. As shown in the introduction, highly social and managed species of bees like honey bees and bumble bees, play key roles in natural and agricultural ecosystems worldwide. Recent losses of bees have been attributed to pesticide exposure, poor nutrition, increased parasite loads and habitat degradation. Over the past several years, governments, beekeepers, and the general public worldwide have become concerned by increased losses of honey bee colonies, calling for more research on how to keep colonies healthy. Our main aim was to explore the complex mechanisms of bees and related species gut bacterial populations, their links to insect immunity and investigate the opportunities for an intervention. Part of our aim was to characterise the bumble bee and wasp gut microbiota using 16S RNA sequencing in a search for potentially novel bacterial species.
We have tried to explain how microbiome interacts with the host and showed that major members of these communities appear to benefit the host. The simple gut communities of social bees present ideal model systems to investigate the underlying evolutionary and genetic processes of such interactions. Information based on our results may help in the design of proper probiotic supplementation strategies with respect to physiological conditions in the honey bee gut.
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Freezing technology of bull sperm in relation to its survivability and fertilization ability
Doležalová, Martina ; Stádník, Luděk (advisor) ; Jiří, Jiří (referee)
The aim of optimalization the insemination doses production is to provide the highest fertilization ability of spermatozoa during the demanding proces of processing fresh semen and its subsequent cryopreservation. Temperature changes causes spermatozoa damage during the cooling and freezing. Spermatozoa is exposed to cold shock and many others limiting factors, which leads to cell death and therefore to decline of fertilization ability of thawed insemination doses. For increasing spermatozoa resistance, exactly the plasma membrane resistance against cold shock was fraction of egg yolk LDL cholesterol (low density lipoprotein) at various concentrations into the comercially produced diluents added. It is believed that LDL acts possitively to plasma membrane and helps to maintain the fertilization ability of spermatozoa after thawing. Following step in the proces of insemination doses production is slow cooling of diluted semen and equilibration, when the straws are store at cooling box for 30 minutes to 240 hours. This period is necessary to penetrate of certain diluent components into the spermatazoa also maintain the balance between their intracellular and extracellular concentration. Also important is subsequent freezing temperature gradient of insemination doses. The most suitable freezing method is based on computer controlled temperature decline in freezing chamber which allows the precise control of ice crystals formation that could tear and kill the cell.
During 2012 to 2016 was repeatedly collected semen from the group of breeding bulls (n = 27, Holstein and Czech Fleckvieh breed) at AI centre. Semen which fulfill the standard entrance conditions in first step was evenly into several parts divided. For dilution the three types of comercially diluents AndroMed, Bioxcell and Triladyl with and without LDL addition were used. Into the diluents AndroMed and Bioxcell the concentration of LDL 4 %, 6 % and 8% into the dilent Triladyl 6 %, 8 % and 10 % was added. Diluted semen was filled into the glass capillares with volume 0,1 ml and temperature +4 °C. Subsequently the sample was placed to cold bath (0°C) for 10 minutes. Then the volume of capillare with physiological solution (37 °C) was mixed and for next 120 minutes was incubate. The effect of cold shock to proportion of live spermatozoa was evaluated by using Eosin and Nigrosine staining technique during heat test of spermatozoa survivability after spermatozoa heating and after 120 minutes of incubation.
The more suitable semen diluents which provide the higher spermatozoa resistance against cold shock were AndroMed and Bioxcell. Together the possitive effect of LDL addition into the diluents to lower decrease of proportion of live spermatozoa during heat test was found (P<0.05). The most suitable LDL concentration which had a favorable influence at spermatozoa resistance against cold shock was 6 % in diluent Bioxcell. Values of the proportion of live sperm were higher at the beginning of the heat test (+1.31% to + 3.2%) and after 120 minute incubation (+5.82% to +8.41%) compared to other diluents with and without addition of LDL.
In the next step the process of equilibration was optimized, is an important part of insemination doses production. The effect of the length of equilibration for subsequent fertilization ability of spermatozoa was evaluated using spermatozoa motility based of CASA and proportion of live spermatozoa after thawing and during heat survival test lasting 120 minutes (37 ° C). Suitable semen was diluted by comercially used diluent AndroMed based on soya lecithin, filled into the straws (0.25 ml), cooled and equilibrated in cooling box for 30, 120 and 240 minutes and freezed in programmable freezing box applying four types of freezing curves differing in temperature rate decline. There was used standard and by producer recommended 3. phase freezing curve, then 2. phase freezing curve, and 3. phase freezing curve with slower as well as rapid decline of temperature rate in freezing chamber, compared with standard freezing curve. The highest spermatozoa motility was found using 240 minutes of equilibration by +2.72% and +4.58% compared to other lengths of equilibration (P <0.05 to 0.01). The highest proportion of live spermatozoa was found using 120 minutes of equilibration (+6.87 % and +8.68 %). The highest average spermatozoa motility during heat test after thawing was achieved by using 2. phase freezing curve (from +2.97% to +10.37%, P <0.05), also in the proportion of live spermatozoa (from + 4.37% to +8.82%, P <0.01). When evaluating interaction between the length of equilibration and freezing curve (standard 3. phase and 2 . phase freezing curve), the highest average spermatozoa motility and proportion of live spermatozoa using 240 minutes of equilibration by both freezing curves was reached, there was no statistically significant differences. As well as, in all evaluated parts of this study the individual differences between ejaculate of bulls and within semen from one bull (P <0.05) as secondary effect were found.
To maintain good fertilization ability of semen during cryopreservation is necessary to increase the spermatozoa resistance against cold shock using addition of correct concentration of LDL into the commercially used diluents AndroMed and Bioxcell. Subsequently the fertilization ability of insemination dose is influenced by cooling, the length of equilibration and freezing. The length of equilibration 120 minutes and more as well as gentle way of freezing according to freezing curve, which ensures a gradual decrease of temperature in freezing chamber provided the higher average spermatozoa motility and proportion of live spermatozoa.
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An analysis of the practical part of the final apprenticeship examinations at a particular apprenticeship school
Ritterová, Miroslava ; Kříž, Emil (advisor) ; Hrušková, Eva (referee)
The bachelor thesis analyses the final practical examination of students at a vocational school a private institute in Prague that trains cooks and waiters who receive full vocational certificate training. The analysis was performed in school years 2012-2013 and 2013-2014 and 2014-2015. The aim of my thesis is to compare the quality and knowledge level of written, practical and oral final examinations. The thesis analyses the results of the tests according to individual assignments. The theoretical part of my thesis describes the individual components of the final examinations, evaluation criteria, final examinations following uniform assignment, and the history of our school. The practical part describes the actual course of all the parts of the final examinations both in their former and contemporary unified assignment form. Results of both forms are then confronted and compared.
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