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The Role of School Psychologists in Primary school
MALEČKOVÁ, Kateřina
The aim of my thesis is to introduce the structure of school counselling. It specifies the individual counselling facilities. It focuses on school counselling and first of all, on the position of school psychologist at primary schools. The particular chapters deal of the history of school counselling, the legislative determination of the position of school psychologist, methods of work which are used for diagnosis of children and youth. At the same time there are mentioned some dangers and difficulties of work of school psychologist. At the end of the theoretical part I reflect some other possibilities in school psychology. The data which I acquired in the research part have enabled me to answer some questions regarding the work of school psychologist at three primary schools in Southern Bohemia, specifically, if school psychologists are accepted by the other teachers. Next I studied the attitudes of social worker and legal protection of children and youth who cooperate with school psychologists.
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Potravní zdroje bezobratlých v Ardovské jeskyni a v jeskyni Domica - předběžné výsledky
Nováková, Alena ; Elhottová, Dana ; Krištůfek, Václav ; Lukešová, Alena ; Hill, P. ; Kováč, L. ; Mock, A. ; Luptáčik, P.
The chemistry (organic matter content and pH) of different substrates from the Ardovská and Domica Caves in the Slovak Karst National Park (Slovakia) and the composition of their microbial communities were studied. Differences in organic matter content and microbial communities were found between the substrates from the Domica and Ardovská Caves. A broad spectrum of photoautotrophic microorganisms (algae and cyanobacteria) was found in different cave microhabitats (cave sediment, rotten wood, earthworms casts and isopods faeces), but no algae were detected in the fresh bat droppings and in the intestinal tract of the isopod .i.M. graniger./i.. Microfungi and bacterial colonies play the primary role in transforming bat guano into a food source for saprophagous and microphytophagous invertebrates.
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Microbiota of the digestive tract of bees and related insect and the influencing facftors
Hroncová, Zuzana ; Havlík, Jaroslav (advisor) ; Kalous, Lukáš (referee)
This thesis consists of six research chapters, out of which, five have already been published in research journals, 1 is in the process of submission and the last chapter presents original unpublished confidential data in the process of manuscript preparation.
The research presented in this thesis concerns the complex mechanisms of bees and wasps immune system focused on microbiota as component of immunity. As shown in the introduction, highly social and managed species of bees like honey bees and bumble bees, play key roles in natural and agricultural ecosystems worldwide. Recent losses of bees have been attributed to pesticide exposure, poor nutrition, increased parasite loads and habitat degradation. Over the past several years, governments, beekeepers, and the general public worldwide have become concerned by increased losses of honey bee colonies, calling for more research on how to keep colonies healthy. Our main aim was to explore the complex mechanisms of bees and related species gut bacterial populations, their links to insect immunity and investigate the opportunities for an intervention. Part of our aim was to characterise the bumble bee and wasp gut microbiota using 16S RNA sequencing in a search for potentially novel bacterial species.
We have tried to explain how microbiome interacts with the host and showed that major members of these communities appear to benefit the host. The simple gut communities of social bees present ideal model systems to investigate the underlying evolutionary and genetic processes of such interactions. Information based on our results may help in the design of proper probiotic supplementation strategies with respect to physiological conditions in the honey bee gut.
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Living with Disability
Stárková, Jana ; Zelinková, Olga (advisor) ; Krahulcová, Beáta (referee)
In my thesis, The Life of a Handicapped Person, I tried to chart what influences the future of such person and how problems trouble him in life. In the first chapters I defined and separated the types of disabilities, their different impact and problems in diagnosis. The central part of the work is the chapter on systems that influence the life of a disabled person. These systems are mostly influenced by the family because, by accepting the child, the parent, as well as the selected type of upbringing, affects the future of the person and likewise the disabled child has a vital influence on the family. Also included is a chapter on health care, social services and education. The quality of life of a disabled person is also influenced by friends, hence a social process, and partnerships, and equally important is the person's professional role. The fourth chapter relates to research which was carried out by a questionnaire on a group of 46 people with physical disabilities. On the basis of the answers, I verified the validity of the six pre-defined hypotheses.
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The nursing process in a patient with chronic pain - usage of non-pharmacological techniques
Janůšová, Veronika ; Voleníková, Ivana (advisor) ; Horká, Eva (referee)
My thesis deals with chronic pain and how a nurse should handle it. This thesis consists of three parts. The first one focuses on pain in general, both on the history and the pain origin mechanisms. It also deals with pain classification. The main topic is chronic pain. Here I reflect basic characteristics of this pain, its influence on human psyche and also necessary cooperation with medical personnel. The end of the first part concentrates on the psychosocial factors playing a very important role in the pain perception: understanding the patients, their behaviour and also accepting their responses to their pain. In the second part I list the pain handling therapeutic procedures. I start with the most widely and frequently used methods, then I deal with the newer ones, which are being introduced nowadays. The focus of the last part of my thesis is the patient suffering from chronic pain. I deal with all aspects of human nature, with the problems and difficulties according to M. Gordon. I specify the relevant nursing diagnosis based on the nursing case history. I make a nursing plan containing the goal, nursing intervention, implementation, evaluation, and also education. The purpose of my thesis was detecting the problems of the patients suffering from chronic pain, coming to the solution together and...
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Crystallographic study of the iron-regulated outer membrane lipoprotein (FrpD) from Neisseria meningitidis
SVIRIDOVA, Ekaterina
Neisseria meningitidis (N. meningitidis) is a Gram-negative commensal bacterium colonizing nasopharynx of about 10 % of healthy individuals, which can cause invasive diseases, such sepsis and meningitis, upon occasional penetration into bloodstream. Pathogenesis of N. meningitidis appears to be directly related to conditions of limited iron availability. Under these conditions two proteins of unknown function: FrpC and FrpD, are synthesized. FrpD is a highly conserved lipoprotein of N. meningitidis anchored to the bacterial outer membrane. It is known that FrpD tightly binds the FrpC protein, which belongs to the Repeat-in-Toxin (RTX) protein family and may act as bacterial exotoxin. However, the mechanism of FrpD-FrpC interaction and the exact function of this complex are unknown due to the absence of structural information on these proteins. Therefore, we set out to determine the structure of FrpD and provide insights into its interaction mechanism with FrpC and structure-functional relationships of these two proteins. We determined the first crystal and solution structures of the FrpD protein. We found that atomic structures of FrpD reveal a novel protein fold. We uncovered the structure-function relationships underlying the mechanism of interaction between the FrpD and FrpC proteins and tested the putative function of the FrpD-FrpC1-414 complex in vitro. Finally, we proposed the putative function of the FrpD-FrpC1-414 complex as a new minor adhesin of N. meningitidis, which mediates the bacterial adhesion to the host epithelial cells and facilitate the colonization. Our work constitutes the first step in clarifying the molecular basis of the FrpD-FrpC interaction and sets the base for further investigation of the role of FrpD and FrpC in the virulence mechanism of N. meningitidis.
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Fluorescent analysis of the differential protein expression in normozoospermic and asthenozoospermic sperm samples
Děd, Lukáš ; Čapková, Jana ; Kubátová, Alena ; Teplá, O. ; Pěknicová, Jana
Asthenozoospermia is one of the main seminal pathologies underlying male infertility. Previous proteomic studies have demonstrated the significant differences in the protein profiles between normozoospermic and asthenozoospermic sperm samples. Since these studies were primarily focused on the identification of differentially expressed proteins by mass spectrometry, we aimed to evaluate the ability of our diagnostic antibodies to detect the differential expression of selected protein markers by fluorescent microscopy and flow cytometry techniques. Therefore, we analyzed sperm samples from 30 men with normal and 30 men with astheno spermiograms, average by the panel of our diagnostic anti-human sperm (Hs) antibodies. Fluorescent microscopy and flow cytometry analysis revealed quantitative differences in the protein abundances between normo and astheno sperm samples, namely, in GAPDHs, evaluated with Hs-8 MoAb, VCP, evaluated with Hs-14 MoAb, and ATP synthase, evaluated with MoAb Hs-36. From the methodological point of view, we observed very high correlation between the data obtained by fluorescent microscopy and flow cytometry techniques and therefore both methods are useful for evaluation of protein differences associated with asthenozoospermia. From the clinical point of view, we observed the strong association of the low sperm motility in the sample with the expression of proteins, playing an important role in sperm energy metabolism (expected), but also with the expression of all tested intra-acrosomal proteins. These findings further demonstrate asthenozoospermia as a complex semen disorder frequently associated with other semen pathologies, which are not diagnosed by basic semen analysis, and the possibility to use monoclonal antibodies as a tool for diagnosis of protein associated sperm pathologies in the semen with the low sperm motility.
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Nursing procedures in taking blood pressure
SEDLÁKOVÁ, Tereza
By blood pressure is meant lateral pressure or the side pressure of a blood column on the side of a vessel. The pressure is created thanks to the action of heart as a blood pump. This pressure is not the same in different parts of the bloodstream. By the blood pressure is meant the arterial pressure or pressure in the artery, so the pressure in big blood vessels. The highest values are reached in the expulsion phase of the heart action, which is called systole. The lowest values are reached in the phase of filling heart chambers, which is called diastole. The sole nursing procedures required in the process of taking a blood pressure reading are many and all must be followed. This thesis is composed of a theoretical part and practical part. Introduction to the theoretical part is composed of the main terminology from the physiology of blood pressure. In the theoretical part we follow with division on hypotension and hypertension, which are the most common diseases related to blood pressure, with diagnosis and recommendation for treatment. This thesis also offers information about measuring blood pressure by the invasive and the non-invasive method. The theoretical part is focused on the importance of the nurse in the process of measuring blood pressure with emphasis on her knowledge and practical skills.
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Role of the nurse in screening for depression in the elderly.
VRZALOVÁ, Monika
The diploma thesis deals with problems of depression in older people. Mainly the work is focused on identifying and analyzing the role of nurses in screening for depression in older people in primary care, acute care, long-term care and home care. This thesis was focused on theoretical direction and was used the method of design and demonstration. In this thesis was set one main goals with five research questions. The main goal was to identify and analyze the role of nurses in screening for depression in the elderly. RQ 1: What is the role of the nurse in screening for depression in the elderly? RQ 2: What is the role of the nurse in the primary care in screening for depression in the elderly? RQ 3: What is the role of the nurse in screening for depression in hospitalized patients in acute care? RQ 4: What is the role of the nurse in screening for depression in seniors in long-term and home care? RQ 5: What rating scales and methods are used in screening for depression in the elderly? The thesis introduce the concept of depression. The following are specified the causes of and the important factors that affect depression in the elderly. It also deals the differences in the clinical symptomatology of depression in old age. It explains possibilities and various barriers in the diagnosis of depression. Another chapter introduces complete geriatric examination, diagnostic classification systems, possible screening methods and scales for detection of depression in the elderly population. It also deals methods of pharmacological and non-pharmacological treatment and its possible complications associated with older age. By reason of increased suicide rate caused by depressive disorder the issue of suicidal behavior in the elderly is introduced. The next chapter deals with the nursing process, which is used by nurses in practice. It consists of the evaluation of the patient's health condition, making nursing diagnosis, creating nursing plan and subsequent implementation and evaluation. The nursing process is also needy for providing quality care. The nursing process in the stage of nursing diagnosis, introduces possible nursing diagnosis for a patient suffering from depression, which are based on the latest classification. Finally is described the role of nurses in screening for depression in the elderly in different health facilities and their contribution to the timely evaluation of depression in the elderly. This chapter introduces the role of nurses, nursing screening and collaboration with a physician. The role of nurses in screening for depression in different medical facilities is based on the first phase of the nursing process of assessment. On the basis of objective and subjective information, the nurse will assess the overall health and mental condition of the patient. Primarily, it was investigated what is the role of the nurse in screening for depression. On the basis of content analysis and synthesis it was necessary to used and processed domestic and foreign literature. A number of relevant sources are the results of various studies and Meta-analyzes, mostly from abroad, but also from the Czech Republic. The thesis can serve as a basis for nurses. The result of this thesis is to create e-learning material available for students in the Faculty of Health and Social Sciences of South Bohemia in Ceske Budejovice in the tutorial called Moodle.
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