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Testing of Marketing Communication
CÍSAŘOVÁ, Alena
The aim of the diploma thesis "Testing of Marketing Communication" is to create a new marketing communication for generic product and to test the consumer´s perception of this communication. In this thesis, salt has been chosen as the generic product because it presents a very common commodity and people need and use the salt every day.
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The creation of videotutorials for the University of Economics, Prague
Zeman, Ondřej ; Šedivá, Zuzana (advisor) ; Kupec, Michael (referee)
The purpose of this bachelor thesis is to acquaint readers with new possibilities of teaching by using information technology and its application, when making learning material. The main outputs of this thesis are video tutorials, which serve as an education support for the course 4IT110 Use MS Excel in business practice taught in the University of Economics, Prague. However, it may be useful to other students of different courses. The process of making tutorials is described from the very beginning by specifying methodology, creating model exercises, subsequent testing of tutorials by students and optimisation of videos according to their suggestions and comments.
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Reasoning for decisions on mobile application testing
Balák, Martin ; Gála, Libor (advisor) ; Beránek, Marek (referee)
Thesis analyses the problematics of determining both qualitative and quantitative parameters for testing on projects involving development of mobile applications. First, content and scope of testing is fixed, followed by the specification of influence model. Methodology is proposed to ascertain the financial and temporal extent of the testing based on obtained influence model.
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Information systems security penetration testing
Klíma, Tomáš ; Doucek, Petr (advisor) ; Čermák, Igor (referee) ; Čapek, Jan (referee) ; Štubňa, Ivan (referee)
The aim of this dissertation thesis is to develop new methodology of information systems penetration testing based on analysis of current methodologies and the role of penetration tests in context of IS/IT governance. Integral part of this aim is evaluation of the methodology. The first part of the thesis is devoted to the presentation of history and current state of research in selected area, definiton of basic terms and introduction of role of the penetration tests. This part is followed by the review of relevant sources and comparative study of current methodologies with a goal to identify their weaknesses. Results from this study are further used as a basis for new methodology development. Classification of IS penetration tests types and testing scenarios are also included. The second part includes design of new methodology, at first its history, structure and principles are presented, then its framework is decribed in high level of detail. In the third part the reader can find (theoretical and practical) validation. The biggest scientific contribution is the methodology itself focused on managment of penetration tests (which is the area currently not sufficiently descibed). Secondary contribution is the extensive review and the comparative analysis of current methodologies. Contribution to the economic and technical (practical) application we can mainly see in the development of new methodology which enables companies to improve management of penetration tests (especially planning, operational management and implementation of countermeasures).
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Building and using small computer networks
Borecký, Jiří ; Pinkas, Otakar (advisor) ; Šmejkal, Ivo (referee)
This thesis deals with the construction and use of smaller computer networks. To build a better and more efficient computer network was used corporate network of parents, which is mainly used for the operation of accounting. The input information was used for generating analysis of the current state of the computer network. Additionally makes a proposal, which leads to improvement of the computer network and then testing whether the proposal was successful.
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Prototyping of mobile apps and their testing in real environment
Ornst, Zdeněk ; Sigmund, Tomáš (advisor) ; Michaličová, Alexandra (referee)
This thesis describes process of designing mobile user interface with regard to the context of use. In this context the discipline of prototyping and its utilization for designing is further examined. Next focus is on the principles of user testing of usability in mobile applications. Building on theoretical knowledge the solution for usability testing of prototypes in real environment is proposed. It aims to more effective process of designing mobile applications. The solution is presented on real case with evaluation of its success.
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Design of marketing strategy in the company Imperium Finance
Petrovič, Marko ; Čermák, Radim (advisor) ; Sova, Martin (referee)
Internet marketing is currently one of the most important components of marketing. However, Internet marketing as a whole is much more important than mere webdesign, mere search engine optimization (SEO) or a simple PPC advertising. Most companies have recently made it throught with a website and that was all their presentation on the Internet. However, the internet is one of the fastest growing technology today and the number of its users is growing every year. Constantly there are new technologies that allow us to have a better user experience and also bring us new ways of presentation and promotion. Most of the companies make a mistake early on and underestimate the preparation, don't know their goals, customers or competitors. Internet marketing is the process and the contents of this process is the recognition, analysis, design, implementation, and ultimately verify that you achieve the goals of the organization. In the theoretical part it is defined internet marketing and its advantages over offline marketing. Briefly sum up the history and major milestones of Internet marketing that shaped its present form. Further, it's described current trends in Internet marketing and detail expands upon the selected Internet marketing tools and tools for evaluating the success of campaigns. The practical part deals with the proposal of marketing strategy in the company Imperium Finance. The main objective is to analyze the requirements of the company Imperium Finance, from analysis to draw specific conclusions and devise a marketing strategy that will support the vision and strategy of the company in the following period. All steps were carried out within the company Imperium Finance on real data from active clients and tested in action. The result is a proposal of a marketing strategy that will drive Imperium Finance, and a marketing plan for the company. The biggest benefit of this work is so real use theoretical knowledge in practice.
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Sperm protein profiles of different mammalian species
Pohlová, Alžběta ; Zigo, Michal ; Jonáková, Věra ; Postlerová, Pavla
Proteins are a substantial equipment of the spermatic cell; therefore, the characterization of sperm proteins is crucial for explanation of molecular mechanisms in the reproduction process. We isolated sperm proteins from different mammalian species - pig, bull, human, mouse, dog and cat. Extracted proteins were separated by SDS-electrophoresis and protein/glycoprotein profiles from epididymal or ejaculated sperm were compared. Additionally, we tested cross-reactivity of antibodies prepared to sperm boar proteins on spermatozoa of other mammalian species using immunofluorescent technique. Our future plan is to compare the protein profiles of sperm during their functional development (epididymal, ejaculated, capacitated) in various mammalian species and identify species-specific sperm proteins with zona pellucida binding activity.
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Information content in vocalization of guinea pigs (Cavia porcellus): individual distinction and recognition of predators
Baklová, Aneta ; Baranyiová, Eva (advisor) ; František, František (referee)
Guinea pigs represent domesticated precocious rodents which became common pets. From the first day after birth they are fully vocal. This thesis was devoted
to the vocalization of pups. The aim of the thesis was to 1) determine the age when the vocal individuality in whistle sound is demonstrable; 2) test possible ultrasonic signals emitted by young guinea pigs; 3) study the antipredator reactions and alarm calls to aerial (bird of prey) and terrestrial (dog) predators and human (control test).
A total of 16 guinea pig pups were tested for vocal individuality, 28 pups
for ultrasonic vocalization and 27 adolescents for predator recognition. When testing vocal ontogeny during the first 9 days after birth, I observed changes in temporal, frequency and parameter of intensity . When I test vocal individuality by cross-validated discriminant function analysis (DFA) based on ten acoustic parameters, calls were classified to correct animals with following success: day 1 = 71.9%, day 3 = 58.8%, day 5 = 53.10%, day 7 = 50.60% and day 9 = 63.10%. The highest frequency in whistle was 30.03 kHz. In predator discrimination reactions as freezing, fleeing and vigilance were observed. In the presence of dog, guinea pigs reacted for the longest time and most frequently by freezing. When confronted with a bird of prey, I observed for a longest time and most often fleeing and then freezing. When exposed to a human, guinea pigs showed mostly vigilance. Almost no vocalization was observed except for two events
of alarm calls - drrr as in the presence of dog and chirrup as reaction to bird of prey.
The following conclusions can be drawn from the presented results: 1) vocal individuality of guinea pigs is demonstrable immediately after birth and the rate
of individually different vocal parameters changes with age; 2) guinea pigs are able
to produce sound up to 30 kHz, i.e. within the ultrasound range, but signals of high frequencies are not crucial for their communication; 3) guinea pigs discriminate between terrestrial and aerial predators, but they emit alarm calls rather rarely.
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Freezing technology of bull sperm in relation to its survivability and fertilization ability
Doležalová, Martina ; Stádník, Luděk (advisor) ; Jiří, Jiří (referee)
The aim of optimalization the insemination doses production is to provide the highest fertilization ability of spermatozoa during the demanding proces of processing fresh semen and its subsequent cryopreservation. Temperature changes causes spermatozoa damage during the cooling and freezing. Spermatozoa is exposed to cold shock and many others limiting factors, which leads to cell death and therefore to decline of fertilization ability of thawed insemination doses. For increasing spermatozoa resistance, exactly the plasma membrane resistance against cold shock was fraction of egg yolk LDL cholesterol (low density lipoprotein) at various concentrations into the comercially produced diluents added. It is believed that LDL acts possitively to plasma membrane and helps to maintain the fertilization ability of spermatozoa after thawing. Following step in the proces of insemination doses production is slow cooling of diluted semen and equilibration, when the straws are store at cooling box for 30 minutes to 240 hours. This period is necessary to penetrate of certain diluent components into the spermatazoa also maintain the balance between their intracellular and extracellular concentration. Also important is subsequent freezing temperature gradient of insemination doses. The most suitable freezing method is based on computer controlled temperature decline in freezing chamber which allows the precise control of ice crystals formation that could tear and kill the cell.
During 2012 to 2016 was repeatedly collected semen from the group of breeding bulls (n = 27, Holstein and Czech Fleckvieh breed) at AI centre. Semen which fulfill the standard entrance conditions in first step was evenly into several parts divided. For dilution the three types of comercially diluents AndroMed, Bioxcell and Triladyl with and without LDL addition were used. Into the diluents AndroMed and Bioxcell the concentration of LDL 4 %, 6 % and 8% into the dilent Triladyl 6 %, 8 % and 10 % was added. Diluted semen was filled into the glass capillares with volume 0,1 ml and temperature +4 °C. Subsequently the sample was placed to cold bath (0°C) for 10 minutes. Then the volume of capillare with physiological solution (37 °C) was mixed and for next 120 minutes was incubate. The effect of cold shock to proportion of live spermatozoa was evaluated by using Eosin and Nigrosine staining technique during heat test of spermatozoa survivability after spermatozoa heating and after 120 minutes of incubation.
The more suitable semen diluents which provide the higher spermatozoa resistance against cold shock were AndroMed and Bioxcell. Together the possitive effect of LDL addition into the diluents to lower decrease of proportion of live spermatozoa during heat test was found (P<0.05). The most suitable LDL concentration which had a favorable influence at spermatozoa resistance against cold shock was 6 % in diluent Bioxcell. Values of the proportion of live sperm were higher at the beginning of the heat test (+1.31% to + 3.2%) and after 120 minute incubation (+5.82% to +8.41%) compared to other diluents with and without addition of LDL.
In the next step the process of equilibration was optimized, is an important part of insemination doses production. The effect of the length of equilibration for subsequent fertilization ability of spermatozoa was evaluated using spermatozoa motility based of CASA and proportion of live spermatozoa after thawing and during heat survival test lasting 120 minutes (37 ° C). Suitable semen was diluted by comercially used diluent AndroMed based on soya lecithin, filled into the straws (0.25 ml), cooled and equilibrated in cooling box for 30, 120 and 240 minutes and freezed in programmable freezing box applying four types of freezing curves differing in temperature rate decline. There was used standard and by producer recommended 3. phase freezing curve, then 2. phase freezing curve, and 3. phase freezing curve with slower as well as rapid decline of temperature rate in freezing chamber, compared with standard freezing curve. The highest spermatozoa motility was found using 240 minutes of equilibration by +2.72% and +4.58% compared to other lengths of equilibration (P <0.05 to 0.01). The highest proportion of live spermatozoa was found using 120 minutes of equilibration (+6.87 % and +8.68 %). The highest average spermatozoa motility during heat test after thawing was achieved by using 2. phase freezing curve (from +2.97% to +10.37%, P <0.05), also in the proportion of live spermatozoa (from + 4.37% to +8.82%, P <0.01). When evaluating interaction between the length of equilibration and freezing curve (standard 3. phase and 2 . phase freezing curve), the highest average spermatozoa motility and proportion of live spermatozoa using 240 minutes of equilibration by both freezing curves was reached, there was no statistically significant differences. As well as, in all evaluated parts of this study the individual differences between ejaculate of bulls and within semen from one bull (P <0.05) as secondary effect were found.
To maintain good fertilization ability of semen during cryopreservation is necessary to increase the spermatozoa resistance against cold shock using addition of correct concentration of LDL into the commercially used diluents AndroMed and Bioxcell. Subsequently the fertilization ability of insemination dose is influenced by cooling, the length of equilibration and freezing. The length of equilibration 120 minutes and more as well as gentle way of freezing according to freezing curve, which ensures a gradual decrease of temperature in freezing chamber provided the higher average spermatozoa motility and proportion of live spermatozoa.
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