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Tick-borne encephalitis - from pathogenesis to therapy
PALUS, Martin
The proposed thesis contributes to the knowledge about tick-borne encephalitis and its pathogenesis. The thesis describes pathogenesis and immunopathogenesis of tick-borne encephalitis, impact of host's genotype in clinical course determination, immune response of patients with acute tick-borne encephalitis, the mechanism of tick-borne encephalitis virus migration into central nervous system and virus interaction with cells of neurovascular unit as well as potential medical interventions.
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Residual cognitive capacity in unconscious patients. Event related potentials and cerebral blood flow study
Holečková, Irena ; Choc, Milan (advisor) ; Polívka, Jiří (referee) ; Rektor, Ivan (referee) ; Stejskal, Lubor (referee)
UNIVERSITÉ CLAUDE BERNARD LYON 1 - LYON Ecole Doctorale Biologie Moléculaire Intégrée et Cognitive Année Universitaire 2007 - 2008 UNIVERZITA KARLOVA V PRAZE Lékařská fakulta v Plzni Vědní obor : chirurgické obory THESE EN COTTUTELLE FRANCO - TCHEQUE Irena HOLEČKOVÁ Neurochirurgické oddělení Fakultní nemocnice v Plzni Capacités cognitives chez les patients inconscients Étude des potentiels évoqués cognitifs et du débit sanguin cérébral Kognitivní kapacita u pacientů s poruchou vědomí Studie kognitivních evokovaných potenciálů a mozkového krevního průtoku Cognitive capacity in unconscious patients Event related potentials and cerebral blood flow study 2008 Ce travail de thèse a reçu le soutien financier de l'Ambassade de France en République Tchèque L'ensemble des travaux a été réalisé dans le Service de Neurophysiologie Clinique et Epileptologie de l'Hôpital Neurologique de Lyon, au sein de l'équipe de l' Unité INSERM 821 et au sein de CERMEP-imagerie du vivant de Lyon, France. Remerciements Le mot remerciement s'avère insuffisant pour exprimer ma reconnaissance envers Madame Catherine Fischer, Chargée de recherche, pour avoir diriger ce travail. Sa compétence, ses conseils ont été essentiels à la réalisation de cette thèse. Je vous remercie de m'avoir «ouvert les oreilles» au domaine passionnant des...
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Estrogen receptor beta (ERβ) in testicular cells and sperm
Dostálová, Pavla ; Žatecká, Eva ; Děd, Lukáš ; Dorosh, Andriy ; Postlerová, Pavla ; Jonáková, Věra ; Dvořáková-Hortová, Kateřina ; Pěknicová, Jana
Estrogen is a steroid hormone that plays an important role during sperm development in the male and female reproductive tract. Estrogen signalling is a complex process that depends on cell milieu and presence of receptors. Thanks to the steroid nature of estrogens, they can pass through the plasmatic membrane and bind to the intracellular estrogen receptors (ERs). Within the cell, there are several pools of ERs. One of them is localized to the cell nucleus and their activation leads to direct or indirect binding to DNA and ultimately to alternation in gene expression (genomic pathway). Other pools of ERs are associated with plasma membrane or are located in cytosol. Activation of membrane associated ERs leads to rapid non-genomic responses. Nowadays, two classical estrogen receptors are known – ERα and ERβ. Since ERβ is a predominant variant in testes, we focused our study on expression of ERβ variants in murine testes and sperm. We detected two variants of ERβ at mRNA level in both, testes and sperm. These variants differ in 54 nucleotids within the ligand binding domain and this variability results in different affinity to estrogen. We analyzed individual testicular cell types (spermatogonia, spermatocytes, spermatids, Sertoli cells) by RT-qPCR. Our results suggest that both ERβ variants are coexpressed in the same cell type and may therefore interact together. This may have consequences in mediating of estrogen signalling. Moreover, ERβ is expressed more in the later stages of spermatogenesis suggesting the role of ERβ in these stages or alternatively in spermatozoa alone. At the protein level, we detected ERβ in nuclear, membrane and cytosolic fraction prepared from testicular tissue suggesting the involvement of both, genomic and non-genomic, pathways of estrogen signaling in testes. In sperm, anti-ERβ antibodies localized ERβ in acrosome region and tail which is in accordance with the known role of estrogen on capacitation, acrosome reaction and motility.
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Effect of feeding flaxseed on the quality of goat milk fat
Malá, Kateřina ; Fantová, Milena (advisor)
Type of feed, its quality, form of adjustments and feeding technique influence the composition and quality of milk. The share of milk components is highly variable, depending on nutrition and animal health. The greatest changes occurring in milk fat content. Milk fat is responsible for the natural flavor of milk. However, in terms of the modern world, human, saturated fatty acids contained in milk fat is considered negative active ingredient with the subsequent development of cardiovascular disease and the development of atherosclerosis in humans. Currently, therefore a growing demand for polyunsaturated fatty acids in milk as a healthy alternative. For this reason, an effort to influence the composition or the content of fatty acids in the milk of dairy animals. One way to significantly increase the content of desired MUFA and PUFA acids in milk fat is the use of oils and oilseeds in the ration. Very positive influence flaxseed, which among other things contains high levels of linolenic acid.
The aim of this work is to analyze and evaluate the milk fat of goats, which are fed with linseed in extruded form compared with a control group of goats without complementary food. It can assume that feeding flaxseed to increase milk fat content, including omega-3 and omega-6 fatty acids.
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Development of immune response to the Hymenoptera venom allergens during specific allergen immunotherapy
Hrabánková, Eva ; Kučera, Petr (advisor)
Order of ants and wasps ( Hymenoptera ) counts a hundred thousand species of insects (only on the lives of our around 15,000 kinds ) 1 . Some individuals have a stinging venom apparatus that they primarily used to defend against the enemy or stun kořisti1 , 2 and 3 stings or bites One can manifest in different ways. Not too often, but severe or even fatal complication of systemic allergic reactions. System allergen immunotherapy respective insects ( " Specific Allergen Immunotherapy " - SAIT ), abbreviated poison Immunotherapy ( " Venom Immunotherapy " - VIT) is the only causal therapy , anti- of a serious or life -threatening IgE -mediated anaphylactic reaction to following stabbing relevant type hmyzu4 . Its purpose is to induce long-term immunological tolerance (specific hyposensitization) to systemically administered alergenům5 . Initial attempts at VIT has been carried out in the 20s of last century , the initially used whole body extracts ( "whole body extreacts " - WBEs ) were due to comparable efficacy to placebo , replaced in the 70s poisonous extracts , which are used for diagnosis and therapy dodnes3 . The mechanism of induction of immunological tolerance through SAIT is complex and not yet fully understood . Probably induces the formation of the so-called " Blocking " IgG antibodies , reduces...
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Women's awareness of cord blood importance
Grézlová, Lucie ; Hlaváčová, Marie (advisor) ; Formánková, Renata (referee)
This bachelor's work explores a general acquaintance about umbilical blood among women. The aim of the work was to find out if women at the age of 18 - 35 know about a donor ship of umbilical blood and its possible utilization. The work is divided into two parts, theoretical and empirical. In the theoretical part there are several anatomical facts providing a better understanding and orientation in the topic given. In details a method of the taking, utilization a preserving possibility of the umbilical blood are described. Further the most frequent usage variations of the gained fragment follow and that is in the form of transplantation. The list of the often complications together with their appropriate cure is not missing. The empirical part was implemented by means of a survey. First of all necessary goals of the research and resulting hypotheses were defined. Then subsequently the questionnaire has been composed and the suitable research pattern has been chosen. More than one half of this work is formed by the individual responses set up mainly into the charts and graphs for a better orientation. In conclusion the obtained information and the whole research progress together with the proposals of a better women's acquaintance in this area are evaluated. Powered by TCPDF (www.tcpdf.org)
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The role of immune cells in tumor growth and spontaneous regression in a rat sarcoma model
Kovalská, Jana ; Chmelíková, Eva (advisor) ; Anna, Anna (referee)
The aim of the thesis is to study differences between sexes of rats in tumour growth, the difference in immune response between rats with progressing and spontaneously regressing tumours compared to control rats and verification of immunological memory. We used Lewis rat model with subcutaneously inoculated sarcoma. Peripheral blood of males showed a higher number of red blood cells, a higher concentration of haemoglobin and higher haematocrit compared to females. Experimental females with inoculated sarcoma had higher levels of CD161 (NK) cells at the beginning of experiment in comparison with the experimental and control males and control females. A strong infiltration of NK cells was observed in tissue sections of spontaneously regressing sarcoma compared to progressing tumours tissue. Important factors that can influence if the animal will show tumour progression or spontaneous regression are neutrophil granulocytes (they can induce both tumour-supporting and anti-tumour response), vascularization and extent of necrosis in tumour tissue. We have verified immunological memory in a small group of females by the secondary inoculations of the same tumour cells after complete disappearance of primary, spontaneously regressing tumour. No sarcoma repeatedly evolved in any of the females. Results obtained in the present work brought new insights into the problems of tumour in the Lewis rat sarcoma model with inoculated sarcoma. These results may have significance also for area of clinical oncology due to similarities with the behaviour of some human cancers.
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The effect of live weight on androsterone and skatole content in adipose tissue of boars
Poláčková, Miroslava ; Okrouhlá, Monika (advisor) ; Michaela, Michaela (referee)
The aim of this study was to provide a comprehensive literature review and evaluation of research on the influence of androstenone and skatole for boar live weight, which thanks to modern trends sensitive issue.
Skatole is malodorous indole compound, which is formed in the colon of pigs later leads to deposition in adipose tissue, causing the so-called. Boar odor.
Steroid androstenone is produced by Leydig cells of the testes boars, when a part is floated urine, partly accumulate in saliva, to stimulate the sows and part is accumulated in the adipose tissue.
The chromatographic method suitable for detecting substances responsible for boar odor was developed on a gas chromatograph mass spectrometer using chemical standards.
According to the results, we can evaluate that the differences between imunocastraties and boars are minimal, not only in fattening values, but also at the height of the back fat or muscle growth musculus longissimus lumborum et thoracis. Only with differing indicators are the amount of androstenone and skatole, which confirms that feeding into higher slaughter weight pigs is unprofitable, because the amount of these substances is many times higher than allowed by European standard. The meat of boars is therefore inconvenient and are a much better choice imunokastráti.
The hypothesis that a live weight of influence on behalf of androstenone and skatole in fat tissue boars are so confirmed.
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Hereditary and non-hereditary factors affecting the quality of rapeseed oil
Zemanová, Kateřina ; Sedláková, Vladimíra (advisor) ; Bečka, David (referee)
This literature review study quality of rapeseed oil in details, depending on heritable and non-heritable factors. Consumption of rapeseed oil is growing every year and in addition to use in human nutrition is also used in animal feed industry or as fuel for motor vehicles. The rapeseed oil has especially a large representation of oleic acid. Furthermore, it is linoleic acid and linolenic acid and undesirable erucic acid, whose quantity is considerably reduced. It is important to mention glucosinolate content, as antinutritional substances, which is reduced together with erucid acid which is known as "00" rapeseed oil. All these components are described in details in this review. The content of fatty acids in rapeseed oil may be influenced both genetic factors and environmental factors. Selection plays a significant role in breeding. Suitably selecting the correct genotypes along with environmental influences can reach the desired target. An important role is played by the synthesis of fatty acids, where in using a desaturation enzymes of fad series, there is desaturation of stearic acid to oleic acid, linoleic acid and linolenic acid. With QTL method, it can be detected genetic background of quantitative traits. Major genes responsible for the quality of rapeseed oil are located on the chromosome A, namely A1, A3, A5 and A8, which are mentioned in the work.
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