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Differences in CO2 Extraction of Vegetable Oils, Essential Oils, and Minor Components from Plants
Sovová, Helena
Significant progress in understanding the phenomena that control supercritical fluid extraction from plants was made in the last years and is reflected in the literature. Combination of chemical engineering approach and botanical knowledge has appeared to be fruitful for the choice of extraction model and even for the prediction of extraction kinetics. This contribution refers to the papers utilizing knowledge of plant microstructure and location of extracted substances together with extraction equilibrium and attempts to specify the differences among extraction kinetics of vegetable oils, essential oils, and other substances. The most abundant substances like vegetable oils in seeds can be regarded in first approximation as non-interacting with plant matrix, in contrast to the minor components whose equilibrium concentration in CO2 is affected by their adsorption on matrix and often also by co-extracted substances.
Fulltext: content.csg -  PDF
Plný tet: SKMBT_C22012102414100 - PDF
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Progress of the system of public health insurance and health insurance companies in the Czech Republic for the period 2000 - 2015
Pecková, Tereza ; Lukášová, Tereza (advisor) ; Bartůsková, Lucia (referee)
This bachelor´s thesis analyzes the basic funds of health insurance companies for the period 2000 - 2015 in the Czech Republic, especially the creation of resources and their use and the entire system of public health insurance in the Czech Republic. Basic funds are one of the components of the overall economy of health insurance companies. First it is necessary to describe the market of the insured. The insured create and use the finances within public health insurance. Also important are contributions from the state budget for those insured by the state. We can conclude that the cost of health insurance companies are rising and that population is aging in the Czech Republic. However, this is not a balanced linear growth. The number of pensioners grew at a slower rate of growth, but according to population development forecast, there should be faster growth in the number of pensioners in the following years. Significant growth in cost is particularly noticeable in the age category of 80 and above, and increasingly more people should live up to this blessed age, also thanks to modern public health.
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Řízení IS/ICT se zaměřením na sourcing služeb informačního systému
Šebesta, Michal ; Voříšek, Jiří (advisor) ; Havlíček, Zdeněk (referee) ; Příklenk, Oldřich (referee) ; Král, Jaroslav (referee)
Research on outsourcing has been around for several decades, while recent evolution in the information systems discipline towards ICT service commoditization significantly changes the context of decision-making. Services that are available on-demand via the Internet allow organizations implementing functions they demand in a fraction of time. This trend represents a chance for organizations seeking to use advanced ICT services without a need of major investments. Problem is the current lack of guidelines and tools for managing ICT services and their outsourcing. Given the trends on the ICT service market, it is expected that much of the IT management in the future will encompass the ICT services and utilize service-level structures. Methods currently available are either too broad or encompass only small part of the whole problem. Ad-hoc or unsound decisions in this area might cause major complications in terms of quality, usability, integration, and consequently influence total cost of organizational IT. Organizations need to either revise existing models or propose and implement completely new models to manage their IS/ICT. This thesis deals with the management of IS/ICT with focus on the ICT services outsourcing. It discusses available sourcing models in the literature and links them to the various interconnected areas. Based on these areas, it presents an integrated view on IT outsourcing strategies. Most importantly the thesis proposes an original concept for decision-making about outsourcing of ICT services named the SOURCER framework. This approach utilizes the presented outsourcing strategies, and introduces a complex methodology and decision-making criteria that will assist organizations with selection of ICT services in order to maintain and manage a most suitable ICT service portfolio. The decision-making is based on four essential viewpoints: function, costs, time, and quality. These viewpoints are discussed, individually analyzed, and serve as a basis for further research. The whole framework is developed and validated according to Design Science Research Methodology (DSRM). Individual components are evaluated using a survey among a group of selected IT managers. Proof of concept is then established by a case study on framework use in a real organization. This case study covers strategy specification, business--IT alignment, specifying service architecture and its interconnections, outsourcing, and management of the ICT service portfolio.
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Expected development of Business Intelligence applications in the coming years
Aschmann, Jakub ; Pour, Jan (advisor) ; Fortinová, Jana (referee)
This thesis focuses on the development of Business Intelligence applications. The main area is the potential development in the coming years, history of business intelligence and individual components. To acquire the necessary knowledge, I studied educational materials and electronic publications. In Chapter 3 I focus on the definition of BI, in the following chapter I analyse individual components and the last chapter is dedicated to the development in the future. This thesis will provide comprehensive knowledge and trends in business intelligence to readers who are interested in this area and are looking for complex information.
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Mléčná užitkovost plemene lacaune ve vybraném chovu
CHODCOVÁ, Anna
This thesis deals with the influence of age of Lacaune breed ewes on milk performance (milk yield and the content of individual components of milk). The length of lactation was also evaluated. Data from farm Statek Horní Dvorce s.r.o. were used and entries from years 2012, 2013 and 2014, when the milk performance was monitored by method AT. With the view of the fact that the standardized lactation in 2012 was set on 240 days while since 2013 the required length was 150 days, the year 2012 was not included into the comparison due to dubiousness of the results. The influence of age of an ewe on total milk yield in the studied farm is not clear and cannot be established with significant certainty, although in the studied years the milk performance was higher than the average in the Czech republic. In the 2013 was the total milk yield 323 kg of milk and in the 2014 was 307,4 kg of milk. The findings of basic milk parameters of individual components of milk show that the age of an ewe has an influence on the components. With growing age the content of fat was decreasing. At three-year-old ewes the content of fat was 7.36%, while at four-year-olds the content reached only 7.3%. The content of lactose also seemed to decrees with age. At three-year-old ewes the content of lactose was 4.86%, while at four-year-olds the content was 4.63%. However, the content of protein had growing tendency. At three-year-old ewes the content of protein was 5.61%, while at four-year-olds the content was 5,89%. Further was evaluated the length of lactation. It was shown an increase in the average length of lactation as they get older ewes, but the effect on lactation length was also the start date of the dry period of herd.
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Effect of low doses of herbicide paraquat on antioxidant defense in Drosophila
SÁBOVÁ, Michala
Disruption of cell equilibrium between production of free radicals and antioxidant defence is named oxidative stress. The main component of antioxidant mechanism is activity of antioxidant enzymes, which include superoxide dismutase (SOD) and catalase. In this study we evaluated the effect of herbicide paraquat on response of SOD and catalase, their transcription level and locomotion activity in Drosophila melanogaster. We exposed the flies to a wide range of paraquat concentration. Our results revealed that transcript and enzymatic levels of both SOD and catalase have a similar biphasic dose response with the peaks at 2,5 ?M paraquat concentration, resembling hormetic effect. Then, males were more sensitive than females. However, females had an increased locomotion activity. We found that paraquat susceptibility is increased in males and mating flies. Therefore, this study supports hypothesis of stress sensitivity elevation as a physiological cost of reproduction.
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Puppies and young dogs nutritions
Šabatová, Kateřina ; Hučko, Boris (advisor) ; Plachý, Vladimír (referee)
Puppies and young dogs nutritions Summary In this work I have attempted to summarize the most important aspects in nutrition of puppies and dogs in growth. The introduction describes the digestive tract and also the changes that have occurred due to domestication. Next is explained the importance of nutrition and water in nutritional requirements of a dog. Important are needs of nutrients and energy that are necessary to maintain the provision of the necessary vital functions. The basic components of food are proteins, saccharides, lipids, minerals and vitamins. I explain the necessary proportions, the amounts and the possible consequences of their lack in food. I focus on essential fatty acids, which in recent years are subject to frequent explorations and their pros and cons are not yet fully understood. I also mention ballast and significant specific active substances. Nutrition of pregnant and lactating females is often neglected, but for puppies it is a very crucial period. The influence of nutrition of pregnant females to the size and weight of newborn puppies has been proved. Further I deal with nutrition of puppies immediately after birth, I explain the composition of colostrum and its effect on the immunity system of the puppy. I also compare ratios of nutrients in the colostrum and milk. Further, I deal with the feeding of the individual at the time of weaning and in different growth periods. There are many possibilities of feeding puppies from after weaning until adulthood described in books. With the new knowledge about nutrition the ways of feeding our dogs have inevitably changed , but also the dogs' nutritional requirements have changed due to the conditions where and how they live and what their nutrient needs are according to their sport, breeding or other use. Currently, there is a possibility of a commercially produced complete nutritional diet or home-prepared fresh food - cooked or raw- barf. In the last chapter, I summarized possible aspects of malnutrition and its potential consequences. I described the most common problems associated with inadequate nutrition of growing dogs such as obesity as well as malnutrition, which also adversely affects the individual. The influence of proper nutrition of the puppy on its growth is clear. If we neglect the nutrition of the dog in the first two years of its life, the consequences are often irreversible.
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Microbiota of the digestive tract of bees and related insect and the influencing facftors
Hroncová, Zuzana ; Havlík, Jaroslav (advisor) ; Kalous, Lukáš (referee)
This thesis consists of six research chapters, out of which, five have already been published in research journals, 1 is in the process of submission and the last chapter presents original unpublished confidential data in the process of manuscript preparation.
The research presented in this thesis concerns the complex mechanisms of bees and wasps immune system focused on microbiota as component of immunity. As shown in the introduction, highly social and managed species of bees like honey bees and bumble bees, play key roles in natural and agricultural ecosystems worldwide. Recent losses of bees have been attributed to pesticide exposure, poor nutrition, increased parasite loads and habitat degradation. Over the past several years, governments, beekeepers, and the general public worldwide have become concerned by increased losses of honey bee colonies, calling for more research on how to keep colonies healthy. Our main aim was to explore the complex mechanisms of bees and related species gut bacterial populations, their links to insect immunity and investigate the opportunities for an intervention. Part of our aim was to characterise the bumble bee and wasp gut microbiota using 16S RNA sequencing in a search for potentially novel bacterial species.
We have tried to explain how microbiome interacts with the host and showed that major members of these communities appear to benefit the host. The simple gut communities of social bees present ideal model systems to investigate the underlying evolutionary and genetic processes of such interactions. Information based on our results may help in the design of proper probiotic supplementation strategies with respect to physiological conditions in the honey bee gut.
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Freezing technology of bull sperm in relation to its survivability and fertilization ability
Doležalová, Martina ; Stádník, Luděk (advisor) ; Jiří, Jiří (referee)
The aim of optimalization the insemination doses production is to provide the highest fertilization ability of spermatozoa during the demanding proces of processing fresh semen and its subsequent cryopreservation. Temperature changes causes spermatozoa damage during the cooling and freezing. Spermatozoa is exposed to cold shock and many others limiting factors, which leads to cell death and therefore to decline of fertilization ability of thawed insemination doses. For increasing spermatozoa resistance, exactly the plasma membrane resistance against cold shock was fraction of egg yolk LDL cholesterol (low density lipoprotein) at various concentrations into the comercially produced diluents added. It is believed that LDL acts possitively to plasma membrane and helps to maintain the fertilization ability of spermatozoa after thawing. Following step in the proces of insemination doses production is slow cooling of diluted semen and equilibration, when the straws are store at cooling box for 30 minutes to 240 hours. This period is necessary to penetrate of certain diluent components into the spermatazoa also maintain the balance between their intracellular and extracellular concentration. Also important is subsequent freezing temperature gradient of insemination doses. The most suitable freezing method is based on computer controlled temperature decline in freezing chamber which allows the precise control of ice crystals formation that could tear and kill the cell.
During 2012 to 2016 was repeatedly collected semen from the group of breeding bulls (n = 27, Holstein and Czech Fleckvieh breed) at AI centre. Semen which fulfill the standard entrance conditions in first step was evenly into several parts divided. For dilution the three types of comercially diluents AndroMed, Bioxcell and Triladyl with and without LDL addition were used. Into the diluents AndroMed and Bioxcell the concentration of LDL 4 %, 6 % and 8% into the dilent Triladyl 6 %, 8 % and 10 % was added. Diluted semen was filled into the glass capillares with volume 0,1 ml and temperature +4 °C. Subsequently the sample was placed to cold bath (0°C) for 10 minutes. Then the volume of capillare with physiological solution (37 °C) was mixed and for next 120 minutes was incubate. The effect of cold shock to proportion of live spermatozoa was evaluated by using Eosin and Nigrosine staining technique during heat test of spermatozoa survivability after spermatozoa heating and after 120 minutes of incubation.
The more suitable semen diluents which provide the higher spermatozoa resistance against cold shock were AndroMed and Bioxcell. Together the possitive effect of LDL addition into the diluents to lower decrease of proportion of live spermatozoa during heat test was found (P<0.05). The most suitable LDL concentration which had a favorable influence at spermatozoa resistance against cold shock was 6 % in diluent Bioxcell. Values of the proportion of live sperm were higher at the beginning of the heat test (+1.31% to + 3.2%) and after 120 minute incubation (+5.82% to +8.41%) compared to other diluents with and without addition of LDL.
In the next step the process of equilibration was optimized, is an important part of insemination doses production. The effect of the length of equilibration for subsequent fertilization ability of spermatozoa was evaluated using spermatozoa motility based of CASA and proportion of live spermatozoa after thawing and during heat survival test lasting 120 minutes (37 ° C). Suitable semen was diluted by comercially used diluent AndroMed based on soya lecithin, filled into the straws (0.25 ml), cooled and equilibrated in cooling box for 30, 120 and 240 minutes and freezed in programmable freezing box applying four types of freezing curves differing in temperature rate decline. There was used standard and by producer recommended 3. phase freezing curve, then 2. phase freezing curve, and 3. phase freezing curve with slower as well as rapid decline of temperature rate in freezing chamber, compared with standard freezing curve. The highest spermatozoa motility was found using 240 minutes of equilibration by +2.72% and +4.58% compared to other lengths of equilibration (P <0.05 to 0.01). The highest proportion of live spermatozoa was found using 120 minutes of equilibration (+6.87 % and +8.68 %). The highest average spermatozoa motility during heat test after thawing was achieved by using 2. phase freezing curve (from +2.97% to +10.37%, P <0.05), also in the proportion of live spermatozoa (from + 4.37% to +8.82%, P <0.01). When evaluating interaction between the length of equilibration and freezing curve (standard 3. phase and 2 . phase freezing curve), the highest average spermatozoa motility and proportion of live spermatozoa using 240 minutes of equilibration by both freezing curves was reached, there was no statistically significant differences. As well as, in all evaluated parts of this study the individual differences between ejaculate of bulls and within semen from one bull (P <0.05) as secondary effect were found.
To maintain good fertilization ability of semen during cryopreservation is necessary to increase the spermatozoa resistance against cold shock using addition of correct concentration of LDL into the commercially used diluents AndroMed and Bioxcell. Subsequently the fertilization ability of insemination dose is influenced by cooling, the length of equilibration and freezing. The length of equilibration 120 minutes and more as well as gentle way of freezing according to freezing curve, which ensures a gradual decrease of temperature in freezing chamber provided the higher average spermatozoa motility and proportion of live spermatozoa.
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Single particle analysis of size-segregated aerosol in Prague city center
Marvanová, S. ; Skoupý, Radim ; Kulich, P. ; Bendl, J. ; Hovorka, J. ; Machala, M.
Particulate matter (PM) is omnipresent pollutant in the ambient air known to cause cardiovascular and respiratory diseases (WHO 2004). Recently, outdoor air pollution and particulate matter in outdoor air pollution were classified as carcinogenic to humans, Group 1 (IARC 2015). Especially, ambient PM of aerodynamic diameter < 100 nm, ultrafine particles, appears to be of great importance due to its high specific surface area and high number concentration (Hughes et al. 1998). Ultrafine particles also easily enter and are being transferred in organisms, and interact with cells and subcellular components (Oberdorster et al. 2005). As the evidence of ultrafine PM significance increased, size-fractionated PMs sampled by various cascade impactors have been employed into the toxicological studies on cell cultures or isolated cells, using the organic extracts of size-fractionated PMs (Topinka et al. 2013, Topinka et al. 2015) or directly the size-fractionated particles (Becker et al. 2003, Ramgolam et al. 2009, Reibman et al. 2002, Loxham et al. 2013, Jalava et al. 2006, Thomson et al. 2015, Jalava et al. 2015). The aim of this study was to evaluate shape and composition of size-segregated aerosol particles, sampled by high volume cascade impactor, using electron microscopy and energy dispersive X-ray spectroscopy (EDX).
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