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Diatoms as bioindicators for tracking freshwater salinization.
Severa, František ; Kopalová, Kateřina (advisor) ; Dobiáš, Jakub (referee)
The focus of this thesis is on the relationship of diatom (Bacilariophyta) communities to conductivity, and the utilization of this relationship for the monitoring of freshwater salinization. Salinization of freshwaters as a consequence of anthropogenic influences is emerging as a threat to many freshwater habitats due to increasing anthropogenic sources of salts (like use of de-icing salts on roads), wastewaters, and fertilizers. The problem is intensifying because salts are further concentrated through increased evaporation as a consequence to climatic changes. Salinization of ecosystems has a direct impact on the physiological processes of present organisms, and because the ability to adapt to this stress differs between organisms, results in the reorganization of communities. In this way, salinization can indirectly threaten ecosystem services that benefit mankind. Furthermore, salinization is a direct threat in terms of its influence on drinking water and crop irrigation. These forces also act on diatoms, which have long been used as bioindicators, and exhibit strong community links with conductivity. Like for other organisms, this tight relationship is caused by differences in adaptation mechanisms between species, which includes osmolytes, extracellular polymeric substances and adaptation of...
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Plant growth on saline soils - interaction of sodium and potassium in plant metabolism
Peřinová, Anna ; Tylová, Edita (advisor) ; Konrádová, Hana (referee)
Soil salinity represents a significant stress factor that negatively affects plant growth and is also a current problem in modern agriculture. This bachelor's thesis focuses on the mechanisms associated with this stress, with a main emphasis on the effects of excess sodium cations in the soil, their accumulation in plants, the mechanism of their negative impact, and defense strategies. It addresses the importance of potassium and sodium in plant nutrition and metabolism, as the interference of Na+ with the essential functions of K+ is an important aspect of the stress caused by salt excess in the environment. The thesis analyzes the most important transporters and channels that ensure the uptake and transport of K+ and Na+ in plants. Emphasis is placed mainly on the transport of sodium and the mechanisms that enable plants to adapt to saline environments and tolerate higher concentrations of salts in the soil, particularly the SOS defense system against Na+ accumulation in the cytoplasm and unique structures that allow salt secretion in halophytic plants. Attention is also directed to the difference between glycophytic and halophytic plants.
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Experimental cultivation systems in root system research
Střelec, Petr ; Soukup, Aleš (advisor) ; Lhotáková, Zuzana (referee)
The growing awareness of plant root importance has contributed to the continuous development of cultivation methods that facilitate more efficient and effective root research. Due to their sessile nature, plants rely extensively on roots for water and ion absorption (Meister et al., 2014). As a result, plant roots must adjust to an array of unfavorable conditions such as salinity, drought, and high temperatures. To better understand root response and promote agricultural development, researchers simulate various stressors in controlled conditions. More recently developed transparent soils and soilless cultures aim to overcome the limitations of traditional soil cultivation, which include imprecise variable control, heterogeneous conditions, and destructive root sampling (Gregory et al., 2009). When planning an experiment, it is crucial to select an appropriate cultivation method to ensure success. The aim of this thesis is to provide a comprehensive overview of the benefits and limitations of commonly used cultivations techniques in plant stress research. Transparent soil reliably mimics the mechanical properties of soil whilst allowing for easy root access and use of numerous visualization techniques. On the other hand, it still maintains the need for a larger cultivation area just like soil. The...
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Photosynthetic parameters as secondary selection traits during plant breeding for salt stress tolerance
Hlavatá, Věra ; Kočová, Marie (advisor) ; Lhotáková, Zuzana (referee)
High salt concentration is important abiotic stress factor that negatively affect plant growth and development and finally also their yield. Increase of plant tolerance to this stress factor is thus one of the most important goals in breeding programmes, particularly in areas with saline environment. Various physiological traits have been suggested for the testing of plant tolerance to improved salinity. Photosynthetic parameters play undoubtedly the main role, because of their rapid response to stress conditions. Generally, photosynthetic rate, chlorophyll content and chlorophyll fluorescence belong to the photosynthetic parameters, that are usually recommended for the evaluation of stress tolerance. All of them were also used for study of salt tolerance in crop plants. The advantages of these measurements are rapidity, non-invasive techniques and particularly, the possibility to test wide collection of genotypes during the short period. Although some of the parameters were recommended for the evaluation of stress tolerance, the results are still ambiguous. The aim of this study was to briefly describe the changes of selected photosynthetic parameters of different plant species in consequence of salt stress and assess the usefulness of these parameters for reliable screening for salt tolerance.
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Utilization of treated wastewater in a drip irrigation system
Šalanda, Pavel ; Innemanová, Petra (advisor) ; Cajthaml, Tomáš (referee)
Drought has an increasing importance in the Czech republic. It is necessary to look for potential irrigation water sources, which are important for agriculture. Treated wastewater can be one of them. It has potentially negative impact on soil physico-chemical properties, which is well-known from many researches in arid regions. The aim of this diploma thesis was to investigate, if the drip irrigation with treated wastewater (from constructed wetland) also has a potentially negative impact on two soils in the Czech republic. In one year experiment drip irrigation with treated wastewater was carried out in the greenhouse. Four treatments of irrigation was used for both soils - irrigation with treated wastewater, irrigation with groundwater, irrigation with treated wastewater + rainwater, irrigation with groundwater + rainwater. An amount of rainwater correspond with total precipitation of two locations, where the soils were brought from (Kostelec nad Ohří - Ústí nad Labem region and Hostětín - Zlín region). pH and electrical conductivity of soil leachate, total concentration of Na, K, Ca, Mg a P, bulk density, particle density and porosity was measured in both soils in 5 and 10 centimeters from source of irrigation. Values of pH of soil leachate fluctuated in every treatment in both soils during the...
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Úloha huminových látek ve zmírňování negativních dopadů působení solí na rostliny ječmene jarního
Jarošová, Markéta
Salinity induced by various sodium salts (NaCl and Na2SO4) led to changes in growth parameters that differs depending on used cultivar and pH of applied salt solution. Cultivar Radegast showed the highest sensitivity to salinity that was reflected in a reduction of shoot and root growth, a decrease of tissue water content, an excessive sodium accumulation in shoots and a radical formation of ROS in the root tips. Subsequent experiment using various types of humic acids revealed that technical humic acid (HA; 6.0 mg . l-1), unlike Lignohumát Max, stimulates NaCl-treated plant growth and simultaneously reduces accumulation of sodium in their tissues.HA applied into culture solution is able to induce an enhancement of certain metabolites(syringic acid and lactic acid in shoots, quinic acid and malic acid in roots, andascorbic acid, reduced glutathione, phytochelatin 2 and free amino acids in shoots). However, NaCl caused a decrease of majority free amino acids (except for proline and histidine), phenolic acids and antioxidants in leaves. On the other hand, an increase of lactic acid and quinic acid was observed in roots. Salinity significantly induced oxidative stress; it is evident from the increased activity of antioxidant enzymes (APX, CAT, GR and SOD). HA was able to mitigate NaCl-induced oxidative stress, as also evidenced by fluorescence microscopy. Simultaneously, HA was also able to reduce NaCl-induced excessive accumulation of sodium content in cultivar Radegast.
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Phenology of mangroves
Hradečný, Jakub ; Koubek, Tomáš (advisor) ; Sklenář, Petr (referee)
The mangroves are a globally important ecosystem with an important value for many organisms, humans included. Many of the organisms are addicted to leaf production, as a part of their diet. These foliovorous organisms are directly affected by mangrove phenology, which presents differences on the global scale. It seems possible that the phenology is influenced mainly by annual temperature oscillations and its changes mark the start and the end of phenological periods. The phenology of mangroves can be influenced by another climatic factor too, as for instance rainfall or salinity. In the latitudes of 20ř, the phenological phases show unimodal mode with the long duration of phenology periods, usually longer than one year. Closer to the equator, the phenology of the plants becomes to change into a bimodal mode, with more than one peak of phenological event per year. In the area of the equator, the phenology of the plants changes into a multimodal mode, without differences during the whole year. This ideal model can be changed by local climatic extremes changing the mangrove phenology into a different mode.
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