National Repository of Grey Literature 17 records found  1 - 10next  jump to record: Search took 0.00 seconds. 
Measurement of force generated by living cells
Lukášová, Veronika ; Skácel, Josef (referee) ; Fohlerová, Zdenka (advisor)
This thesis deals with methods used to measure cell traction forces. In order to study and measure the forces it is important to understand the mechanotransduction process. This is described in the theoretical part of the thesis along with an explanation of the adhesive capacity of cells. Next are presented the methods used to monitor traction forces: collagen contraction, tissue pillars, traction force microscopy in 2D and 3D, and measurements on micro- and nano-pillars. The practical part of the thesis is focused on determining the magnitude of the traction forces, which can be calculated from the knowledge of the pillar deflection and the spring constant of the material. The practical part includes the processing of the light microscope images and the resulting procedure for calculating the magnitude of the traction forces.
Fabrication of plasmonic array of gold nanostructures for biomolecule detection
Tesák, Filip ; Fohlerová, Zdenka (referee) ; Lednický, Tomáš (advisor)
This work deals with the preparation of gold nanostructures for future usage in biomolecule detection. In this case, gold nanostructures specifically stand for gold nanowires, which are formed on the basis of electrical deposition of gold into porous templates. These templates can be prepared by anodic oxidation of aluminum, with particular emphasis on its purity.
Microconductometer for salinity measurement
Paštěka, Marek ; Fohlerová, Zdenka (referee) ; Hubálek, Jaromír (advisor)
This Bachelor Thesis deals with designing and construction of microconductometer for measuring salinity of electrolyte. Sensor with flexible base is placed on top of human skin. Holes inside the flexible base collects sweat leading to capillary system. All capillars leads to sensor area where its conductivity is measured.
The influence of modified TiO2 nanotubes on biointerfacial interaction
Bílek, Ondřej ; Kočí,, Kamila (referee) ; Hlaváček,, Antonín (referee) ; Fohlerová, Zdenka (advisor)
Nanotrubičky oxidu titaničitého v průběhu posledních let nabyly na významu v poli biomedicíny. Jakožto biokompatibilní nanostrukturovaný povrch nachází potenciál pro své uplatnění především v oblasti implantačních aplikací. Teoretická část této práce je tak věnována různým přístupům pro syntézu TiO2 nanotrubiček, jejich modifikacím a aplikacím v biomedicíně. Experimentální část pak pojednává o nanotrubičkách oxidu titaničitého, které jsou připraveny z titanu metodou jednokrokové anodické oxidace v organickém elektrolytu. Jako výchozí materiály jsou používány křemíkové disky s naprášenou vrstvou titanu a titanové folie. Zprvu amorfní nanotrubičky jsou žíháním převedeny na svou krystalickou podobu, a následně modifikovány selenovými a stříbrnými nanočásticemi. Připravené struktury jsou zkoumány z hlediska povrchových vlastností a biologických interakcí s vybranými tkáňovými kulturami (MG-63, NIH-3T3) a bakteriemi (E. coli, P. aeruginosa, S. aureus). V závěru experimentální práce jsou stručně porovnány výsledky selenových a stříbrných nanočástic. Hlavním cílem této práce je rozšířit znalosti týkající se bio-rozhraní tvořeným adherentními buněčnými liniemi, bakteriálními buňkami a nanostrukturovaným povrchem tvořeným TiO2 nanotrubičkami dekorovanými selenovými a stříbrnými nanočásticemi.
Detection of single photon-upconversion nanoparticles by luminescence microcopy
Polachová, Natálie ; Kolář, Radim (referee) ; Fohlerová, Zdenka (advisor)
This bachelor thesis deals with the detection of photon-upconversion nanoparticles using the U-net convolutional neural network, by using epiluminescence microscopy. The theoretical part contains an introduction to the issue of photon-upconversion, description and use of photon-upconversion nanoparticles. Furthermore, the thesis deals with the functioning of basic and convolutional neural networks. In the practical part, we prepared samples of nanoparticles with subsequent acquisition of images by epiluminescence microscopy. The convolutional neural network U-net was designed, which further serves for the detection of nanoparticles bz using H-maxima morphological operations. In the end, everything was summarized and statistically evaluated..
Study of mesenchymal stem cell migration in the extracellular matrix based on principles of chemotaxis
Scholasterová, Viktorie ; Fohlerová, Zdenka (referee) ; Chmelíková, Larisa (advisor)
This thesis engages in a study of mesenchymal stem cell migration in extracellular matrix based on principles of chemotaxis. First, attention is focused on a theoretical part associated with a clarification of basic terms such as extracellular matrix, migration, confocal microscopy, mesenchymal stem cells or chemotaxis. There is also included a list and a description of some basic methods for monitoring cell migration and a more detailed description of a method called transwell assay, which has been chosen for an experiment in a practical part of this thesis. This part includes protocols of individual steps for the preparation of the experiment, the procedure of data processing obtained by scanning cells with a confocal microscope and a description of the resulting confluence values.
Study of disassembly/reassembly mechanisms of ferritin protein cages and their utilization in nanomedicine
Krausová, Kateřina ; Fohlerová, Zdenka (referee) ; Heger, Zbyněk (advisor)
Diploma thesis deals with the study of dissociation and reassociation of ferritin protein cages and their use in nanomedicine. Most studies that are focused on targeted transport of pharmaceuticals using ferritin cages work with horse spleen ferritin. It is, however, its origin, which leads to increasingly frequent questions about possible immunogenicity in the patient's organism, which also provides the main motivation to test the possibility of encapsulation of low-molecular drugs into ferritins originating from alternative organisms. In the practical part the method for the study of dissociation was experimentally designed. Native polyacrylamide gel electrophoresis was used to study dissociation of equine ferritin composed of different subunit, human ferritin, and archeal Pyrococcus furiosus ferritin. The obtained subunit dissociation results were used to encapsulate the low molecular chemotherapeutic drug doxorubicin and for further characterization of the ferritin-doxorubicin complex. The efficacy of the designed nanoformulations has been verified in the treatment of malignant breast cancer. Human ferritin proves to be the optimal one. Its composition of heavy subunits corresponds to a lower protein stability, thus a more efficient opening of the structure and consequent encapsulation of the cytostatics occurs. With its 60% encapsulation efficiency of doxorubicin, low polydispersity index, effective cytotoxicity of ferritin-doxorubicin complex and minimal risk of immune response to the patient's organism, human ferritin achieves better results than commonly used horse spleen ferritin.
Nanostructured sensors for detection of organic compounds
Šťávová, Karolína ; Fohlerová, Zdenka (referee) ; Hrdý, Radim (advisor)
This bachelor's thesis is focused on the fabrication of sensitive layers composed of an array of gold nanorods and their modifications. Firstly, a nanoporous mask is made from a layer of aluminum using a method called anodic oxidation. Afterwards electrochemical deposition of gold and polypyrrole or gold and mercury is carried out into the mask. The sensitive layer is characterized by the scanning electron microscopy and other methods for elemental analysis. A sensor with this type of sensitive layer can be used for the detection of various organic compounds.
Study of expression of transferrin receptors (TfR1) and their utilization in nanomedicine
Krausová, Kateřina ; Fohlerová, Zdenka (referee) ; Heger,, Zbyněk (advisor)
Bachelor thesis deals with the expression of the transferrin receptor (TfR1) and its use in nanomedicine. During the last decade, nanotechnology emerged as one of the central milestones in connecting all scientific and technological disciplines. Nanomedicine already demonstrated efficacy not only in animal models of cancer but also in clinical practice. The theoretical part is not only aimed at cancer of the human population, but also at the possibilities of targeted drug delivery into the tumor tissue, which greatly reduces the otherwise serious side effects of conventional treatment – systemic toxicity. The practical part is focused on optimization for studying the expression of the transferrin receptor, a protein overexpressed by neoplastic cells aiming to enrich the higher metabolic needs of tumor cells. The optimal conditions were as follows: lysate of 50 000 cells applied with nonreducing nondenaturing buffer and the concentration of the primary antibody of 1.0 𝜇g/ml. Different levels of TfR1 expression were detected, depending on the type of tumor cells. The cell lines of neuroblastoma, prostate cancer (occurence in every 7th man) and breast cancer (occurence in every 8th woman) were selected for the next experiments. Via this transferrin receptor, apoferritin, which is a protein storaging iron ions in many organisms, can be internalized into cells. Artificially, the internal cavity of apoferritin may be used for encapsulation and transport of any molecules. In the case of this bachelor thesis, the apoferritin was used for delivery of doxorubicin. Doxorubicin has been used for cancer treatment for more than 30 years; however, its administered dose is limited by its high toxicity. This can be reduced by its encapsulation in a suitable vector for targeted transport to the tumor cells only. Apoferritin could serve as such suitable vector. In this thesis, the suitable usage of apoferritin as a nanocarrier for chemotherapeutic delivery was confirmed. Its molecule size of 10-12 nm allows it to employ the effect of increased permeability and retention. At the same time, this size makes it possible to avoid renal clearance. The properties of encapsulated doxorubicin are not affected by apoferritin, thus preserving its toxicity for cells with a high level of TfR1 expression (30% growth inhibition of these cells after 24 h of treatment).
Ruthenium-based nanoparticles and testing of their anticancer activity
Žáková, Eliška ; Fohlerová, Zdenka (referee) ; Heger,, Zbyněk (advisor)
Neoplastic diseases hold the second place of the most common causes of death worldwide. Available treatments include various combinations of surgery, chemotherapy, radiation, hormone therapy, immune therapy and targeted therapy. The emphasis is currently laid on nanomedicine, where new nanosized complexes are developed and applied for the targeted treatment and chemotherapy. The aim is to significantly improve the anticancer effect and decrease the damage to organism. In this thesis, ruthenium nanoparticles with a size of 12–14 nm were synthesized and their surface modified with polyvinylpyrrolidone. Furthermore these were subsequently modified with polyoxyethylene(40)stearate for binding of doxorubicin. These nanoparticles were tested on breast carcinoma cells (MDA-MB-231), ovarian carcinoma cells (A2780) and neuroblastoma cells (UKF-NB-4). Apoptosis and necrosis testing showed 60—64 % increase in apoptosis when comparing ruthenium nanoparticles modified with doxorubicin to nonmodified ruthenium nanoparticles. The modification increased level of oxidative stress in tumorous cells and slightly a genotoxicity to non-tumorous cells, nevertheless the hemocompatibility was significantly improved. Testing has proven with IC50 0.98 g/ml, 3.91 g/ml and 1.95 g/ml higher sensitivity to these cells and confirmed expected anticancer activity. Compared to one of the most common chemotherapeutic agents cisplatin the modified ruthenium nanoparticles are significantly more toxic to cell lines A2780 (IC50=21 µg/ml), MDA-MB-231 (IC50=9 µg/ml) and UKF-NB-4 (IC50=4 µg/ml).

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1 Fohlerová, Z.
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