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Electrochemical biosensors with spatially separated enzymatic and detection parts for selective analysis in flow-through arrangement
Tvorynska, Sofiia ; Barek, Jiří (advisor) ; Labuda, Ján (referee) ; Korecká, Lucie (referee)
This dissertation thesis presents the newly developed four highly reusable, stable as well as simple, and cost-effective electrochemical (bi)enzymatic biosensors for the selective and reliable determination of choline, acetylcholine, uric acid, and L-lactic acid in flow injection analysis. All biosensors are based on the concept of the spatial separation of the biorecognition part from detection one and amperometric monitoring of the enzymatically consumed oxygen via its four-electron reduction at the highly negative detection potential. In this way, the design of the biosensors includes an easily replaceable enzymatic mini-reactor(s) connected upstream to the flow cell that contains the appropriate silver amalgam-based transducer. The enzymatic mini-reactor based on choline oxidase, uricase, or lactate oxidase was used for choline, uric acid, or L-lactic acid biosensors, respectively. The acetylcholine bienzymatic biosensor includes the consequently connected choline oxidase- and acetylcholinesterase-based mini-reactors. The first part of this thesis focuses on the construction of two different silver amalgam-based electrodes. Specifically, this section discusses the fabrication of a silver solid amalgam electrode covered by mercury film operating in a wall-jet cell and also highlights the...
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The impact of intervention breathing programme on breathing economy during increasing load
POUL, Daniel
Breathing is essential part of our life. Breathing exercise can help acquirement of proper breathing. Thanks to this process, sports performance can be improved. The aim of our work is to determine differences in values and compare results of spiroergometric parameters from testing on a bicycle ergometer in the laboratory of functional load diagnostics. We obtained the results by an entrance test and a final test tested after eight weeks. Between testing, selected individuals performed a respiratory intervention program. Nine performance university athletes, especially focused on football, got involved in our research. Data at the rest position and load were crucial. We focused on dynamic ventilation parameters such as tidal volume, respiratory rate, minute ventilation or oxygen consumption. In the output test, we also recorded changes in values in the respiratory segments, abdominal, thoracic and subclavian areas. We verified the results from the point of view of material and statistical significances. Both of these significances were evaluated in this thesis. The materially and statistically significant differences in the results of measurement were improved in the oxygen consumption by our probands. The studied probands were able to improve in various phases of load, for example, the material significance was detected at the respiratory rate during the load of three watts per kilogram. Ascertained results were not improved in all monitored parameters. The most significant changes were discovered in the three-watt load during the respiratory rate from 32,8 +- 7,2 to 30,9 +- 5,1 breaths per minute. Probands reduced oxygen consumption by 14.8 percent.
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Physiological response and physical activity during climbing wall in school age children
Kalábová, Monika ; Panáčková, Michaela (advisor) ; Strejcová, Barbora (referee)
Title of master thesis Physiological response and physical activity during climbing wall in school age children Work objectives Determinaton of specific oxygen uptake during climbing in the climbing wall in children. Methods The study involved 10 boys and 9 girls. Their climbing ability was in range 4 to 5+ degree of UIAA (Union Internationale des Associations d'Alpinisme). Maximal oxygen consumption was measured on climbing wall. The climbers climbed two routes, the first vertical profile (90ř) and second overhanging profile (110˚). The participants climbed the wall with a self-selected speed. They climbed the route twice to steady state of physiological response during climbing and to simulate real average wall (15 m). To make conditions equal, everybody had 30 s to get down and start climbing again. Results The girls achieved average of specific oxygen consumption VO2peak 37,1 ± 4,8 ml·kg- 1 ·min-1 in the vertical profile and in the overhanging profile was 40,6 ± 11,5 ml·kg- 1 ·min-1 . The boys achieved slightly higher average of specific oxygen consumption in both profile 39,1 ± 4,8 ml·kg-1 ·min-1 in the vertical profile and 42,1 ± 2,6 ml·kg-1 ·min-1 in the overhanging profile. The girls had average peak heart rate 179 ± 10 beats·min-1 in the vertical profile and boys 170 ± 14 beats·min-1 ....
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The energy performance at the growth rate in ski-mountaineering
Hepnar, Jan ; Vomáčko, Ladislav (advisor) ; Jindra, Matouš (referee)
Title The energy performance at the growth rate in ski-mountaineering Objectives Measure group skialpinists of different ages and performance of the ski-mountaineering simulator with a gradient 21ř and speed 1,4 km/h to 5,4 km/h with increasing 0,4 km/h in every minute. Determine their energy expenditure in different speeds as the speed increases and with constant slope. Methods Seven ski-mountaineerers of different levels were testing in laboratory of ski-mountaineering simulator. We calculate of maximum oxygen consumption and anaerobic threshold. Results The probands attained average energy expenditure 8,18 kJ per minute at the end of the first minutes and they attained average energy expenditure 29,32 kJ per minute at the end of the 10th minutes. The average energy expenditure of probands increased by 2,35 kJ per minute an average with a standard deviation 0,69 kJ per minute. Keywords ski-mountaineering, heart rate, oxygen consumption, ski-mountaineer trainer.
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Comparison of human physiological response in different one-rope ascending techiques
Doležal, Jan ; Michalička, Vladimír (advisor) ; Baláš, Jiří (referee)
Title: Comparison of the physiological response rate of the organism using different one-rope ascent techniques Objectives: The aim of this study was to compare the rate of the physiological response of the organism using 3 different one-rope ascent techniques during constant speed. Methods: It was an empirically based study of experimental character (quasi-experiment). The measured research group (n = 12) consisted of the military students (21 ± 1.1 years; 183 ± 4.9 cm; 80.6 ± 7.8 kg) of full-time study at the Faculty of Physical Education and Sport of Charles University (VO FTVS UK). The physiological response rate was measured with the Cortex Metamax 3b and Polar sporttester during one-rope ascending techniques ("using Prusik knot" = P", "using jümar = B", "using Garda knot = G") at a constant speed of 3 m·min-1 for 7 minutes. The rating of the perceived exertion was also recorded on the Borg RPE scale. The data were comparatively analyzed in the SPSS statistic program, furthermore the analysis of variance (ANOVA) was also employed. Results: The study demonstrated statistical differences (p ≤ 0.05) between techniques: while B is the easiest, the greatest differences were observed between technique B, P and G. The G technigue appeared to be the most difficult. The average HR was: 162 ± 9 bpm...
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Effect fo different sizes of wheels of in-line skates on energy expenditure
Sukovič, Richard ; Jindra, Matouš (advisor) ; Brtník, Tomáš (referee)
EFFECT OF DIFFERENT SIZES OF WHEELS OF IN-LINE SKATES ON ENERGY EXPENDITURE Objectives: The aim of this bachelor thesis is to determine the difference in energy expenditure when using different sizes of wheels of in-line skates. Methods: The research group consisted of 5 people in age 26,4 ± 2,2 years. This was an experiment. The calculation of energy expediture was based on the amount of oxygen consumption (VO2) and exhaled carbon dioxide (VCO2), i.e. indirect calorimetry. For the analysis of respiratory gases MetaMax 3B measuring device was used. Three sizes of wheels of the same hardness were tested. The rides were carried out on a 1,5 km section of a cycle path with the effect of the height difference of 2 mm/m. Probands completed one test ride (with a cadence of 70 rpm) on each size set of wheels. Test ride lasted for 5 minutes. For results were processed values of 3 minutes steady state VO2. Results: We found out a noticeable difference in energy output between 72 mm and 90 mm large wheels. The difference between driving on 72 mm and 80 mm large wheels and driving on 80 mm and 90 mm large wheels was, due to the standard error of measurement, insignificatnt. Conclusion: The energy expenditure reached a noticeable increase in driving with a larger diameter of wheels compared to driving on...
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Metabolice response of indoor rock climbing and treadwall climbing
Hlaváček, Lubomír ; Baláš, Jiří (advisor) ; Gajdošík, Jan (referee)
Title: Metabolic response of indoor rock climbing and treadwall climbing Objectives: The goal of this work is to compare the differences between an indoor rock climbing and treadwall climbing in terms of the metabolic response of the organism. Methods: Forty-two climbers of three different performance levels participated in the research, who during seven days underwent in random order, separate measurements on an indoor wall and a climbing simulator at a speed of 4 m.min- 1 to a height of 19.5 meters with a slope of 90˚. The values of the metabolic response of the organism were monitored, specifically oxygen consumption (VO2), minute ventilation (VE), respiratory rate (DF), respiratory exchange ratio (RER), heart rate (HF), energy consumption (ES) and rating of perceived exertion (RPE). Results: Climbers of lower performance level were reaching average values of VO2, VE, DF, RER and ES higher than advanced climbers, both on the indoor wall and the an treadwall. In terms of average EC values, the indoor wall is more demanding for all groups (lower performance: treadwall 0.59 ± 0.07 kcal·kg-1 , indoor wall 0.69 ± 0.08 kcal·kg-1 ; intermediate: treadwall 0.57 ± 0.09 kcal·kg-1 , indoor wall 0.64 ± 0.11 kcal·kg-1 , advanced: treadwall 0.56 ± 0.08 kcal·kg-1 , indoor wall 0.62 ± 0.06 kcal·kg-1 )....
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Oxygen consumption in awake cardiac surgical patients
Pořízka, Michal ; Stříteský, Martin (advisor) ; Szárszoi, Ondrej (referee) ; Šetina, Marek (referee)
OBJECTIVES: Standard blood flow rates for cardiopulmonary bypass have been assumed to be the same for awake cardiac surgery with thoracic epidural anesthesia as for general anesthesia. However, compared to general anesthesia, awake cardiac surgery with epidural anesthesia may be associated with higher oxygen consumption due to missing effect of general anesthetics. This may result in insufficient oxygen delivery and lactic acidosis when standard blood flow rates were used. The primary aim of our study was to investigate if standard blood flow rates are adequate in awake cardiac surgery. The secondary aim was to evaluate postoperative clinical outcomes of patients undergoing awake cardiac surgery. METHODS: Forty-seven patients undergoing elective on-pump cardiac surgery were assigned to receive either epidural (Group TEA, n=17), combined (Group TEA-GA, n=15) or general (Group GA, n=15) anesthesia. To monitor adequacy of standard blood flow rates, arterial lactate, acid base parameters, central venous and jugular bulb saturation were measured at six time points during in all groups. Blood flow rates were adjusted when needed. Subsequently, early and late postoperative outcome data including hospital and 3-year mortality was recorded and compared among the study groups RESULTS: No lactic acidosis has...
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Comparison of load during diagonal striding and double poling on the roller ski during sprint
Čechová, Anna ; Suchý, Jiří (advisor) ; Gnad, Tomáš (referee)
Title: Comparison of internal loading on the organism by double poling and diagonal striding during the sprint on the roller ski Objectives: The aim of the diploma thesis is to compare the internal loading using the Cortex Metamax 3B and the SenzoStar GL devices in a two-hundred meters long section performed by the maximum effort between the double poling and diagonal striding about the 10.4% climb on roller skis. Methods: The diploma thesis is conceived as an empirical field research, the aim of which is to compare a double poling and diagonal striding in terms of internal loading on roller skis during sprint. The test subject will be a group of cross-country skiers and long-distance skiers. Results: Using the measured values of the internal loading and the measured time, we found differences during comparing the diagonal striding and double polling in specific values of SF, VO2 / kg, VE. the conclusion was that diagonal striding is faster than double poling to the uphill on roller skis. Keywords: XC-skiing, roller skis, Cortex MetaMax 3B, oxygen consumption, heart rate, lactate
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