|
|
Isolation of photosystem II particles and their characterization
Svitačová, Denisa ; Pekař, Miloslav (referee) ; Venerová, Tereza (advisor)
This master's thesis deals with the isolation and characterization of spinach thylakoid membranes and photosystem II particles. Based on the research, two procedures were chosen for the isolation of spinach thylakoid membranes and photosystem II particles with an emphasis on preserving their native properties. Subsequently, the characterizations were carried out, focusing on the spectroscopic properties of the isolated samples. UV-VIS spectrometry was used to determine the ratio of chlorophyll a to chlorophyll b in samples of thylakoid membranes and photosystem II particles. The success of the isolations was confirmed by fluorescence methods. The excitation and emission spectra of the samples were measured by the steady-state fluorescence spectroscopy, and the fluorescence lifetimes of the samples of thylakoid membranes and photosystem II particles were determined by time-resolved fluorescence. The isolated samples were afterwards visualized with a fluorescence microscope with a CCD camera and the FCS technique.
|
|
|
pH sensitive fluorescence probes
Marková, Kateřina ; Obruča, Stanislav (referee) ; Mravec, Filip (advisor)
The goal of this thesis was to suggest the suitable method for measuring bacterial cytosolic pH in bacteria strain Cupriavidus necator. Fluorescent microscopy was chosen to obtain scan of bacteria and time-resolved fluorescence was chosen to obtain a calibration curve. BCECF-AM was used as a pH-sensitive fluorescent probe. It was suggested that the external calibration is more suitable than internal one for the prokaryotic type of cells. Bacteria H16 shows a long fluorescence lifetime only on the granules containing PHB, while in PHB-4 the longest fluorescence lifetime occurs randomly throughout the cell.
|
|
| |
|
|
Software FLIM system in confocal microscopy
Petrula, Jakub ; Odstrčilík, Jan (referee) ; Čmiel, Vratislav (advisor)
This bachelor thesis deals with the FLIM system utilization in confocal microscopy. It describes basic concepts which are closely related to the topic, such as principles of fluorescence, fluorescent dyes and principles of both fluorescence and confocal microscopy. Practical part involves work with the confocal microscope and set of intensity images acquisition of both the animal and plant cells. Final product of pratical part is fluorescence lifetime image. The thesis also describes an algorithm for generating a pseudocolor frames and created user interface.
|
|
|
Software FLIM system in confocal microscopy
Petrula, Jakub ; Odstrčilík, Jan (referee) ; Čmiel, Vratislav (advisor)
This bachelor thesis deals with the FLIM system utilization in confocal microscopy. It describes basic concepts which are closely related to the topic, such as principles of fluorescence, fluorescent dyes and principles of both fluorescence and confocal microscopy. Practical part involves work with the confocal microscope and set of intensity images acquisition of both the animal and plant cells. Final product of pratical part is fluorescence lifetime image. The thesis also describes an algorithm for generating a pseudocolor frames and created user interface.
|
|
|
pH sensitive fluorescence probes
Marková, Kateřina ; Obruča, Stanislav (referee) ; Mravec, Filip (advisor)
The goal of this thesis was to suggest the suitable method for measuring bacterial cytosolic pH in bacteria strain Cupriavidus necator. Fluorescent microscopy was chosen to obtain scan of bacteria and time-resolved fluorescence was chosen to obtain a calibration curve. BCECF-AM was used as a pH-sensitive fluorescent probe. It was suggested that the external calibration is more suitable than internal one for the prokaryotic type of cells. Bacteria H16 shows a long fluorescence lifetime only on the granules containing PHB, while in PHB-4 the longest fluorescence lifetime occurs randomly throughout the cell.
|
|
| |
|
| |
guest ::
