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Generation and analysis of double deficient transgenic mice for kallikrein-related peptidase 5 and kallikrein-related peptidase 14
Hanečková, Radmila ; Sedláček, Radislav (advisor) ; Fulková, Helena (referee)
Kallikrein-related peptidases (KLKs) constitute a highly conserved serine protease family. Based on in vitro experiments, KLKs are predicted to play an important role in a number of physiolog- ical and pathophysiological processes. However, their role in vivo remains not fully understood, partially due to a lack of suitable animal models. In this work, we aim to prepare a KLK5 and KLK14 double-deficient mouse model. Both KLK5 and KLK14 were proposed to be involved in epidermal proteolytic networks critical for maintaining skin homeostasis. However, both KLK5 and KLK14 single-deficient mouse models show minimal or no phenotype, likely due to similar substrate specificity resulting in functional compensation. Double-deficient mice cannot be easily obtained by crossing due to localization of the Klk5 and Klk14 genes within the same locus on chromosome 7. We report that KLK5 and KLK14 double-deficient mice were success- fully generated, mediated by transcription activator-like effector nucleases (TALENs) targeting Klk14 by microinjection of TALEN mRNA into KLK5-deficient zygotes. Furthermore, we show that KLK5 and KLK14 double-deficient mice are viable and fertile. We believe that these novel mouse models may serve as a useful experimental tool to study KLK5 and KLK14 in vivo.
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Rhythms of restlessness
Hudecová, Stanislava ; Kadlčák, Šimon (referee) ; Mikyta, Svätopluk (advisor)
In my bachelor thesis Rhythms of restlessness I adress, through personal associations and memories, the themes of work ethics and the capitalist-oriented culture of today's society. In an installation made up of a series of objects and drawings embedded within them, I materialize my intimate attitudes or feelings by combining moments from memories with moments from everyday routine activities that are an overlooked part of our everyday "autopilot" functioning. The office chair here acts as a memory, metaphor or symbol of working, and its various manipulations across the installation carry references to my associations associated with this phenomenon. Through this work, I explore the relationships of my immediate surroundings to what it means to function in today's success-oriented rather than happiness-oriented time, and it also serves as a way for me personally to become aware of my own place in the context of my artistic practice. The work builds on my previous projects, in which I have often explored themes of my own intimacy and psychology in the context of functioning in today's world. It is closely related to my most recent work entitled Bedroom Routines, which metaphorically worked through domestic routines and moments, following a stylized character who is my "avatar" in the work.
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Connection
Kynclová, Iveta ; Bartošová, Zuzana (referee) ; Korbička, Pavel (advisor)
Iveta Kynclova's bachelor thesis will be based on the search for the relationship between lability and adrenaline, evaluating personal experiences related to riding a motorcycle. The work will include an appropriate choice of materials, allowing both the transmission of personal feelings and the creation of the resulting spatial objects or installations.
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The Quiet Omnipresence
Bodlák, Ondřej ; Dvořák, Vojtěch (referee) ; Mikyta, Svätopluk (advisor)
The work, entitled The Quiet Omnipresence, themes the metaphor of grid and line as representing the source elements necessary for the growth of whole forms. It relies on a study of landscape and urban scenery, its quiet ubiquitous mechanisms and processes. The jumble of lines creates a sensorially elusive monochromatic surface that the observer can encounter when looking at organic structures for longer periods, as well as industrially tuned urban compositions. It explores the relationship of order and chaos in its opposite but paradoxically mutually inexorable connotations. The work mostly relies on a specific and not uncommon type of space often right in the middle of cities – vague terrains, places forgotten and deliberately overlooked, in which banal biological processes slowly take the helm determining the direction of a seemingly random development.
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The Order Processing in The Selected Company
Urban, Dalibor ; Petříček, Aleš (referee) ; Milichovský, František (advisor)
The bachelor thesis deals with the course of the contract in the selected company PRAVAN, spol. s.r.o. On the basis of the information obtained, it describes its current situation, from the receipt of the order, through the entire production process, to the final dispatch of the finished product. The aim of the bachelor's thesis is to propose corrective measures that will lead to optimization of the order process in the company and minimization of delays in contract deadlines.
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Time synchronization by IEEE1588 protocol
Hříbek, Šimon ; Arm, Jakub (referee) ; Fiedler, Petr (advisor)
The thesis focuses on implementing time synchronization using the PTP protocol on the STM32H747 platform to assign precise timestamps to acquired samples using the ADC converter. The chosen platform is specifically the STM32H747 Discovery Kit development board. The thesis includes a description of available libraries necessary for implementing time synchronization. One area also covers the procedure for setting up the Ethernet interface available on the development kit. The thesis also addresses the selection of suitable methods for evaluating the achieved results.
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Fragile Future
Jedličková, Eliška ; Sobotka, Jiří (referee) ; Gabriel, Michal (advisor)
Fragility, variability, immateriality... In my final master's thesis, I focused on the theme of fragility and shadow in visual art. I directed my initial idea towards the motif of fragility and transience. It's a topic that has interested me for quite some time. Where are the boundaries of fragility? How can the perception of this concept differ for each of us? Fragility can be understood as a certain material property, but also as an internal matter. In connection with this theme, I decided to work with shadow, which, for me, is in many ways fragile, elusive, variable, and has no material essence, real nature, or form. I thus chose to work with aesthetic and spiritual phenomena.
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Ritual -between visual Art and Theatrical drama
Janda, Jakub ; Pfeiffer, Jan (advisor) ; Tsikoliya, Lenka (referee)
The diploma thesis deals with ritual as a link between the artistic and theatrical worlds. The theoretical part explores the broad field of rituals, which serves as a starting point for the pedagogical task. In the didactic part, the emphasis is on viewing art from the perspective of the theory of the "Gesamtkunstwerk", whereby pupils can be introduced to other topics in culture, art and history through art activities. Based on research into the field of ritual, the opera Rosenkavalier was selected as a suitable topic for didactic transformation. It is not only possible to focus on the issue of rituals, but also to explore the Baroque and Rococo periods, the reign of Maria Theresa, or to go into the field of literature through the work of Hugo von Hofmannsthal, the author of the libretto, by learning about opera as a dramatic and musical theatre form and the work of Richard Strauss, the author of the music for this opera. The author's part of the thesis focuses on the forms of drawing and its use in relation to dramatic art. KEYWORDS ritual, drama, opera, baroque, rococo, art series, drawing, performance, space, time
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Time management in construction company managerial work
Jirátková, Marie ; Hub,, Karel (referee) ; Linkeschová, Dana (advisor)
The goal of the thesis is to find out if manager in the area of building uses the methods of time management for the planning of work and tasks. At the beginning of theoretical part is the basic definition of the „time management“, assessment of the goals and the priorities, explanation of the methods used by time management and how to manage effectively. This part of the thesis deals with the downside as well – procrastination. Shows this downside as inability to manage the time management. The methods of time management are described and history of this methods as well. The second – practical part of the thesis – contains proposed hypotheses what investigate how the time management is in reality used by building managers, how helps the electronics and if managers prefer the working time. The author tries this hypotheses to confirm or to refute using the survey and structured interviews in selected building companies. The results of the thesis are suitable recommendations for more effective planning of the time and avoidance the mistakes in this area.
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