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Smart meter simulation on KYPO platform
Morawiec, Daniel ; Gerlich, Tomáš (referee) ; Lieskovan, Tomáš (advisor)
This bachelor thesis is focused on problematics of smart grids, smart meters and protocols which are used in the energy industry. The part of thesis is dedicated to DLMS protocol and COSEM specification. Cloud platform OpenStack and cyber range platforms, more specifically KYPO CRP, are subsequently described in this work. In the last part of the bachelor thesis deployment of platforms OpenStack and KYPO CRP is executed and testing scenario with common elements of energy networks is commissioned on the platform KYPO.
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Detection of Farthest Node in Wireless Sensor Networks
Pfudl, Tomáš ; Morávek, Patrik (referee) ; Šimek, Milan (advisor)
Master’s thesis in the first part focuses on the effectiveness of communication in the selection of reference nodes during the anchor-free localization in WSN. In this part two anchor-free localization algorithms AFL and CRP are explored. Knowledge learned on the selection of reference nodes and communication complexities of these algorithms are used in the second part. The second part is aimed at eliminating weaknesses in communication and subsequently proposed a new algorithm for selection of references. The principle of the newly proposed algorithm CASRU (Centralized Algorithm for Selection of Reference Nodes) is based on the principle of CRP. CASRU algorithm builds logical tree structure and then filters the communication through it. New algorithm achieves much greater success in the selection in randomly distributed networks while reducing the amount of communication load.
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lmportance of epicardial fat in heart diseases: new mechanism of action and their therapeutic implication
Matloch, Zdeněk ; Haluzík, Martin (advisor) ; Maruna, Pavel (referee) ; Lipš, Michal (referee)
It was documented earlier that epicardial fat is a source of pro-inflammatory mediators under basal conditions (Mazurek, Zhang et al. 2003, Baker, Silva et al. 2006). It was also found that during cardiac surgery there is an increased expression of mRNA for pro- inflammatory cytokines in both epicardial and subcutaneous adipose tissue (Kremen, Dolinkova et al. 2006). During advanced heart failure, an inflammation of adipose tissue and resistance to adiponectin was also discovered (Khan, Kato et al. 2012). As part of the project, we tested the hypothesis that changes in levels of pro-inflammatory and anti-inflammatory cytokines, including their expression in adipose, muscle and heart tissue, as well as changes in insulin resistance and lipid profile parameters, are dependent on the type of underlying disease of patients, either the presence of CAD or valve degeneration. The aim of our project was to investigate metabolic changes in various fat tissues, insulin resistance, pro-inflammatory and anti-inflammatory cytokines plasmatic levels and their mRNA expressions in patients with coronary artery disease (CAD) undergoing either elective coronary artery bypass surgery (CABG) or isolated valve replacement. Our patients underwent several blood tests and measurements like anthropometric, biochemical, and...
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The Impact of Metabolic Syndrome on the Expression of Inflammation and Cellular Damage Parameters in Relation to the Presence of Psoriasis
Holmannová, Drahomíra ; Fiala, Zdeněk (advisor) ; Dlouhý, Pavel (referee) ; Kujovská Krčmová, Lenka (referee)
Metabolic syndrome is a chronic metabolic and systemic inflammatory disease that is associated with high morbidity and mortality. The increasing prevalence of metabolic syndrome highlights the need for a deeper understanding of the pathophysiological mechanisms and their interrelationship with common comorbidities, including psoriasis. By analysing changes in the levels of selected parameters of inflammation and cellular damage, the knowledge of the pathogenesis of the inflammatory process of both diseases and their combination can be expanded. The results may contribute to effective secondary prevention and primary care interventions and facilitate modification of therapies. Data for the doctoral thesis were obtained from three separate studies, for which a set of parameters reflecting metabolic status, inflammatory processes, and cell damage (oxidative DNA/RNA damage, calprotectin, angiopoietin-like protein 8 /ANGPTL8/, clusterin, elafin, chromosomal aberrations, CRP, and vitamin D) were selected. The studies included groups of people with metabolic syndrome, persons with psoriasis, persons with psoriasis and metabolic syndrome, and healthy subjects. A total of 248 subjects participated in the study in three studies (122, 85 and 41 subjects). Laboratory determination of parameters was performed by...
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Production of human milk oligosaccharides in the cell factory of E. coli
Havrdová, Jana ; Bojarová, Pavla (advisor) ; Smrček, Stanislav (referee)
Human milk oligosaccharides (HMOs) are among the most abundant components of human breast milk and are essential for the development of neonatal health. It is very challenging to isolate these oligosaccharides from animal milk, especially the less abundant ones. Therefore, different approaches are to be sought after. Chemical and enzymatic syntheses of these compounds are labor-intensive, and expensive affording low yields. A newly adopted approach to HMO synthesis is through bac- terial cell factories, in which genetically engineered bacterial strains can use cheap carbohydrate substrates and convert them into specific oligosaccharides. The aim of this thesis is to examine the feasibility of using selected bacterial strain (E. coli) for the production of HMOs. With lactose as a glycan substrate, the bacterial host has to be β-galactosidase deficient, otherwise, the substrate would be degraded. In order to generate higher lactose intake in the cell, a crp gene that encodes for the positive transcriptional regulator (Catabolite Activator Protein - CAP) can be incorporated into the host organism. The cloned plasmid - pRSFDuet- 1-crp was used for the transformation of into the selected bacterial strain. Lactose from the cells was purified by gel chromatography and the influence of CAP over expression...
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The importance of determining the prognostic COVID score for the stratification of patients with COVID-19.
Mirská, Klára ; Fátorová, Ilona (advisor) ; Pešková, Eliška (referee)
COVID-19 remains a life-threating disease with a very heterogenous clinical presentation for risk patients. Recently, there has been an effort to find a suitable laboratory marker for early and reliable assessment of prognosis of this disease. The scoring systems seem to be suitable markers with more complex evidential value. This diploma thesis is engaged in the importance of COVID-19 prognostic score then, which was developed and validated by the Sysmex Europe GmgH corporation in Hamburg (a subsidiary of Sysmex Corporation in Kobe) in the collaboration with European university hospitals. It is a score calculated from ten hematological parameters, which were proven to change dynamically at the onset of COVID-19. This score and the changes in selected hematological and biochemical parameters were observed depending on the course of COVID-19. The observed laboratory markers included WBC, Lymf, Neu, Eo, IG, Delta-He, DDI, FBG, NLR, CRP, PCT, IL-6, ferritin and troponin T. The theoretical part of the diploma thesis is engaged in the general characteristic of COVID-19, the description of its causative agent, the virus SARS-CoV-2, the diagnostics, therapy, and prevention of the disease. The experimental part is engaged in the statistical analysis of the results of the individual observed parameters and...
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Biochemical predictors of the treatment failure in pediatric IBD patients
Zárubová, Kristýna ; Bronský, Jiří (advisor) ; Hrdý, Jiří (referee) ; Petrášová, Miroslava (referee)
Up to 20 % of patients with IBD (inflammatory bowel disease) is diagnosed in childhood, the course of the disease tends to often be more severe and the treatment more difficult than in case of adults. Therefore, it is necessary to commence or eventually change the treatment in order to prevent development of complications. Early detection of relapse plays significant role for further course of the disease and general prognosis of the patient. Changes to laboratory parameters (e.g. to the level of fecal calprotectin) can significantly prevent the development of clinical symptoms and may indicate the necessity for control endoscopy, further escalation or change in therapy. The primary focus of this thesis are patients who underwent surgical bowel resection, who constitute one of the risk groups. Studies show that as many as 70 % of patients suffering from Crohn's disease reach the point of bowel surgery within the first 10 years of the diagnosis. According to the results, a promising predictor of relapse of the disease seems to be serum albumin, which may relate to the overall nutritional status of the organism. We have also examined the effectivity of using fecal calprotectin and CRP as relapse predictors. Testing directly in the resected bowel did not prove usability of calprotectin as a predictor,...
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Changes in selected laboratory parameters in patients with complicated COVID-19
Sýkorová, Klára ; Fátorová, Ilona (advisor) ; Pešková, Eliška (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Author: Bc. Klára Sýkorová Supervisor: Mgr. Ilona Fátorová Title of diploma thesis: Changes in selected laboratory parameters in patients with complicated COVID-19 The aim of the diploma thesis is to write a research on the topic using available literary publications. Comparison of changes in selected laboratory markers in COVID-19 disease in hospitalized patients and patients with a mild course of the disease and then statistical evaluation of the obtained data. The theoretical part contains a briefly description of the COVID-19 disease and its originator - SARS-CoV-2 virus. It deals with the description of the disease, transmission, pathogenesis and the course of the disease. Furthermore, risk factors for the serious course of the disease, its complications, clinical and laboratory diagnostics and finally treatment. In the experimental part of the work, a statistical analysis of the measured data is performed. Comparable hematological and biochemical parameters include WBC, lympho #, NLR, Ret-He, ICIS score, sN # (abs), alG # (abs), D-dimers, fibrinogen, CRP, PCT and myoglobin. A statistically significant difference was found for all selected parameters except WBC. Patients with severe disease...
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Smart meter simulation on KYPO platform
Morawiec, Daniel ; Gerlich, Tomáš (referee) ; Lieskovan, Tomáš (advisor)
This bachelor thesis is focused on problematics of smart grids, smart meters and protocols which are used in the energy industry. The part of thesis is dedicated to DLMS protocol and COSEM specification. Cloud platform OpenStack and cyber range platforms, more specifically KYPO CRP, are subsequently described in this work. In the last part of the bachelor thesis deployment of platforms OpenStack and KYPO CRP is executed and testing scenario with common elements of energy networks is commissioned on the platform KYPO.
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Penetration of B-lactam antibiotics into cerebrospinal fluid and its relationship to inflammatory markers during invasive bacterial infection
Prášil, Petr ; Buchta, Vladimír (advisor) ; Marešová, Vilma (referee) ; Boštík, Pavel (referee)
Penetration of β-lactam antibiotics into cerebrospinal fluid and its relationship to inflammatory markers during invasive bacterial infection Aim: The penetration of β-lactam antibiotics (ceftriaxone, crystalic penicillin G - potassium salt and cefotaxime) into the cerebrospinal fluid (CSF) was estabilished and the correlation between the penetration and laboratory markers of inflammation was evaluated. Materials and methods: Levels of β-lactam antibiotics in serum and CSF were determined in 24 patients (76 samples). In all patients (n = 24) the CSF/serum ratio for antibiotics was calculated and it was correlated with laboratory markers of inflammation (C-reactive protein, fibrinogen, and CSF numbers of neutrophils). Besides, CSF was examined by a modified bactericidal test in patients (n = 13) with positive bacterial culture. In 14 patients (28 samples) levels of serum antibiotic concentration, both before and after its administration, were measured. Results: Serum levels of ceftriaxone before and after administration (31.2 mg/l ± SD 12.29 and 300.0 mg/l ± SD 125.9, respectively) differed with a statistical significance (p = 0.000156). Further, a significant difference between value of inflammatory markers and value of CSF/serum ratio (Kl/s) of ceftriaxone was observed. Patients with ratio values...
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