|
| |
|
|
Isolation and study of the properties of recombinant pseudoosmotin
Richterová, K. ; Nováková, Martina ; Antošová, Z. ; Neubauerová, Tereza ; Macková, Martina
Antimicrobial peptides represent new clinical compounds with antimicrobial action and they could participate in solving the problem of ever-increasing resistance of microorganisms to the traditional antibiotics. The aim of this work was to study optimal conditions for expression, isolation and purification of recombinant pseudoosmotin from Escherichia coli BL21 (DE3). Pseudoosmotin was isolated from membrane fraction of bacteria and was purified by the affinity chromatography using Ni-NTA. Isolated pseudoosmotin did not show antimicrobial activity against studied microorganisms.
|
|
|
Larvae of flesh fly Neobellieria bullata as a source for novel antimicrobial peptides
Neubauerová, Tereza ; Macková, Martina ; Macek, Tomáš ; Šanda, Miloslav ; Voburka, Zdeněk
The antibiotic resistant strains of microorganisms are increasing threat for public health. Antimicrobial peptides from natural sources display a wide spectrum of activity. In our laboratory, several peptide fractions with antimicrobial acivity against gram-positive and gramnegative bacteria and fungi were isolated from larvae of flesh fly Neobellieria bullata. The presence of peptides was proved by tricine electrophoresis, molecular weights and sequences determination by MS and Edman sequencing, respectively. In comparison with database sequences we obtained structures present mainly in insect and amphibian antimicrobial peptides, but total matches were not higher than in 36%. Upon the obtained sequence motifs we designed structures of several synthetic peptides. These were synthetised on the Rink amide MBHA resin. Synthesis was performed according Fmoc/PyBOP-DIEA protocol. Antimicrobial activity of these peptides was determined.
|
|
|
Isolation of antimicrobial peptides and proteins from tomato
Králová, M. ; Šanda, Miloslav ; Macková, M. ; Macek, Tomáš
Recent emerging problem is occurrence of multiresistant pathogens. This inconvenience makes people to search for new antimicrobials. One of possible ways is looking for natural antimicrobial peptides. In our project, green tissues of tomato (Solanum lycopersicum) were chosen for isolation of such peptides. Extracts obtained were fractionated by solid phase extraction and liquid chromatography. Antimicrobial activities were tested on model microorganisms. Several fractions exhibited antimicrobial activity against bacteria and fungi. In the extracts, precursors of two antifungal proteins were identified.
|
|
| |
|
|
Design and synthesis of specific colevelin derivatives for in vitro and in vivo studies
Kostomoiri, M. ; Zikos, C. ; Benaki, D. ; Slaninová, Jiřina ; Pirmettis, I. ; Papadopoulos, M. ; Pelecanou, M. ; Livaniou, E.
Colivelin is the most potent member of the humanin family of neuroprotective peptides with in vitro and in vivo action against insults associated with Alzeimer’s Disease. The mechanism of action of colivelin is not yet elucidated. The objective of the present study is the design and synthesis of specific colivelin derivatives that can be used as molecular probes in in vitro and in vivo biological experiments. In the frame of the study three colivelin derivatives bearing suitable tags, i.e the fluorescent moiety FITC, the 99mTc-radiometal chelating unit dimethylGly-Ser-Cys, and the streptavidin-counterpart biotinyl-group, were designed and synthesized. These derivatives will be used in in vitro cell component binding and in vivo biodistribution experiments, in order to gain better insight on the mode of the neuroprotective action of colivelin.
|
|
|
In vitro and in vivo antimicrobial effect of lasioglossins on the Candida albicans
Kašperová, A. ; Turánek, J. ; Čeřovský, Václav ; Raška, M.
Lasioglossins represent a new group of amphipathic α-helical peptides with significant antimicrobial effect on the Candida albicans. This study examines the antifungal activity of two peptides LL-III and all D-LL-III as measured by the suppression of Candida proliferation and suppression of induced morphological differentiation both in in vitro and in vivo assays. In the in vitro Candida proliferation assay, the inhibitory effect of lasioglossins LL-III and all D-LL-III was more than 70% within 24 h and more than 84% after 48 h of incubation (final concentration of either peptide was 17.5 .mu.M). Delaying of blastoconidial transition to hyphae in vitro and tendency to suppress vaginal candidiasis in experimental mice were detected.
|
|
|
Different charged short lipopeptides
Ježek, R. ; Slaninová, Jiřina ; Králová, M. ; Macková, M.
Series of short peptides palmitoylated in different positions was designed, synthesized and tested for antimicrobial activities. The palmitoyl group was situated either on the alpha - amino group in position 1 or on the epsilon - amino group of lysine in position 3. The peptide charge ranged from –3 to +4. The peptide part of the lipopeptides constituted mainly from lysine, arginine and glutamic acid.
|
|
| |
|
| |
guest ::
