Original title: Isolation and study of the properties of recombinant pseudoosmotin
Authors: Richterová, K. ; Nováková, Martina ; Antošová, Z. ; Neubauerová, Tereza ; Macková, Martina
Document type: Papers
Conference/Event: Biologically Active Peptides /12./, Praha (CZ), 2011-04-27 / 2011-04-29
Year: 2011
Language: eng
Abstract: Antimicrobial peptides represent new clinical compounds with antimicrobial action and they could participate in solving the problem of ever-increasing resistance of microorganisms to the traditional antibiotics. The aim of this work was to study optimal conditions for expression, isolation and purification of recombinant pseudoosmotin from Escherichia coli BL21 (DE3). Pseudoosmotin was isolated from membrane fraction of bacteria and was purified by the affinity chromatography using Ni-NTA. Isolated pseudoosmotin did not show antimicrobial activity against studied microorganisms.
Keywords: antifungal activity; antimicrobial peptides; osmotin; recombinant proteins
Project no.: CEZ:AV0Z40550506 (CEP), GAP501/11/1650 (CEP)
Funding provider: GA ČR
Host item entry: Biologically Active Peptides. 12th Conference, ISBN 978-80-86241-44-9

Institution: Institute of Organic Chemistry and Biochemistry AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0204347

Permalink: http://www.nusl.cz/ntk/nusl-81018


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Research > Institutes ASCR > Institute of Organic Chemistry and Biochemistry
Conference materials > Papers