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The use of immunochemical methods in the diagnostic of cancer and degenerative disease
Hanousková, Lenka ; Kotaška, Karel (advisor) ; Bronský, Jiří (referee) ; Štern, Petr (referee)
Introduction: Immunochemical methods have significant importance in biomedical research. The benefit of immunochemical methods is their high sensitivity and accuracy. The aim of the work was to use immunochemical methods in investigation of cancer biomarkers and degenerative diseases. A total of four studies were performed. Two studies were focused on the search for new biomarkers of prostate cancer, the third study was focused on evaluation of FGF23 in patients with hyperparathyroidism, and the fourth study looked at the assessment of hyaluronic acid in synovial fluid of patients with knee gonarthrosis. Methods and patients: Study 1: Concentrations of Chromogranin A, Endoglin, TIMP-1 and thymidine kinase 1 (TK1) in serum and Endoglin, SPINK-1, Annexin, TIMP-1 in urine were measured in 58 patients with the (mean age 68 years, range 45 - 82 years) with clinically diagnosed prostate cancer and in 30 healthy individuals with the (mean age 64 years, range: 55 - 78 years) without clinically relevant urological history and PSA values in the reference range. Subsequently, serum TK1 levels were measured in 169 patients (mean age 62 years, range 45- 82 years) with prostate cancer and in a control group of 39 healthy men (mean age 61 years, range 54-78 years). The TK1 assay was also used to determine the...
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Rheology of high viscous food substances
Štern, Petr ; Vlasák, Pavel
Rheological properties of high viscous food substances, mainly of animal origin, greatly influenced their preparation, homogenization and processing. By collagen substances were studied the complex of rheological properties by their processing. We have determined, that the viscoelasticity have the bigger influence that dynamic viscosity.
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Rheology and texture of sun lotions
Štern, Petr ; Panovská, Z. ; Morávková, Tereza
All analysed sun lotions were stable. Psychorheology was applied as a suitable complex method. Rheological parameters were compared to sensory texture attributes. All relationships were statistically significant (correlation coefficients 0.89 – 0.99). Fast and objective rheological analysis could replace time and money consuming sensory assessment.
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Psychorheology of cosmetics
Štern, Petr ; Panovská, Z. ; Váchová, A. ; Pokorný, J.
Texture belongs to the most important manufacture qualities of dispersive cosmetics. The psychorheology was used for the determination of relationships between results of instrumental and sensory measurement. Eye creams, sun and body lotions, lipsticks and tooth pastes were investigated. By all this cosmetics products the psychorheology was applied as a suitable complex methods. On grounds of rheological measurements can be therefore the sensory texture and acceptability of dispersive cosmetic predicted.
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Psychorheology of ketchup
Štern, Petr ; Pokorný, J. ; Panovská, Z. ; Šedivá, A. ; Vykutilová, T.
A series of twelve ketchups picked up at the Czech markets was analysed at the temperatures 230C and 450C. There were determined the basic rheological characteristics (static yield stress, apparent viscosity, thixotropy, and viscoelasticity) using a rotational rheometer RheoStress 300 (TermoHaake Karlsruhe, Germany) and textural properties using sensor analysis. With the help of correlation of rheological and sensorical data there were derived close functional dependencies with large values of correlation coefficients.
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