|
|
Effect of endoglin blockage on inflammation-induced endothelial dysfunction in human aortic endothelial cells
Škubová, Andrea ; Tripská, Katarína (advisor) ; Svobodová, Zuzana (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Title of Diploma Thesis: Effect of endoglin blockage on inflammation-induced endothelial dysfunction in human aortic endothelial cells Author: Andrea Škubová Supervisor of Diploma Thesis: PharmDr. Katarína Tripská, Ph.D. Aim: The aim of this thesis was to describe the effect of TNF-α and LPS-induced inflammation on the expression of endoglin and its transcription factors, the expression of biomarkers of endothelial dysfunction, the levels of soluble endoglin, and the adhesion and transmigration of monocytes through human aortic endothelial cells. We further investigated the effect of endoglin blockage by the monoclonal antibody TRC105 (carotuximab) on these processes. Methods: In this thesis, human aortic endothelial cells (HAEC) were used, and they were exposed for 16 hours to TNF-α (10 ng/ml) and LPS (100 ng/ml) and for 12 hours to TRC105 (300 µg/ml). The mRNA expression of endoglin and its transcription factors (KLF6, RelA, NR1H3), mRNA expression of inflammatory adhesion molecules (ICAM-1, VCAM-1, SELE) and mRNA expression of MMP-14 enzyme were measured by real time qRT-PCR. Using flow cytometry, we detected protein levels of endoglin, protein levels of inflammatory adhesion molecules...
|
|
|
Feasibility Study of the Reconstruction of the Building Construction
Svobodová, Zuzana ; Bohm, Rudolf (referee) ; Korytárová, Jana (advisor)
The aim of this work is the explanation of the issue of passive houses and renovation of existing buildings to passive energy standard. Another task of the work is a feasibility study of building renovation of the "Tuzex" for the needs of Center of sustainable construction in Brno. Part of this study is to create a marketing analysis, design of organizational project management, a description of technical solution, impact assessment project on the environment and in particular evaluating the economic efficiency of investment. The result of this work is to evaluate the feasibility of the project under current conditions.
|
|
|
Sentence representations with similarity interpretation
Svobodová, Zuzana ; Hudeček, Vojtěch (advisor) ; Libovický, Jindřich (referee)
Sentence representations - embeddings - obtained from neural network models are the core part of many applications in both academia and industry. Although embeddings reach great results in correlation with human sense of sentence similarity, there is often a lack of explanation for why models choose sentences to be similar. In this thesis, we strive to increase the interpretability of model embeddings by incorporating different semantic sentence level annotations in the learning process. We introduce a model called SBERTslice that produces embeddings that can distinguish nuanced semantic variations in text, including elements like negation, sentiment, named entities, emotional tone, and verb-oriented relation between words in a text. We evaluated SBERTslice embeddings in various text classification and semantic sim- ilarity tasks and for a majority of them, SBERTslice outperformed the original SBERT. 1
|
|
| |
|
|
Detection of soluble endogline in Lab-in-Syringe
Ilićová, Marie ; Svobodová, Zuzana (advisor) ; Chocholouš, Petr (referee)
The main target of the thesis was to develop an automated method for the isolation of endoglin from a sample using a magnetic immunosorbent in Lab-In-Syringe with subsequent immunodetection by affiblot. The theoretical part deals with endoglin, in its membrane and soluble form, especially with their connection to endothelial dysfunction. The principles of anti-endoglin therapy using the monoclonal antibody carotuximab or TRC105 are also briefly described here. Furthermore, the theoretical part summarizes the basics of the automation in Lab-In-Syringe and of the affiblot technique, their principles, and their applications. The last chapter deals with the biofunctionalization of magnetic particles by antibodies in order to prepare a magnetic immunosorbent which is used for the isolation of proteins from a complex sample. The experimental part describes methods for testing the affiblot prototypes. The main goal was to develop a method for the preparation of anti-endoglin magnetic immunosorbent and to increase the capacity of the method by upscaling it from a 1 ml to a 5 ml syringe. Furthermore, methods for the isolation of the soluble form of human endoglin from the culture medium containing the drug TRC105 were developed for 1 ml and 5 ml syringes. Various experiments were carried out to optimize the...
|
|
|
The Phenomenon of Responsibility in the Postmodern Era
Jirgl, Sebastian ; Hogenová, Anna (advisor) ; Semrádová, Ilona (referee) ; Svobodová, Zuzana (referee)
The dissertation The Phenomenon of Responsibility in the Postmodern Era discusses the phenomenon of responsibility in the context of the postmodern era. The issue of responsibility is viewed here primarily from a philosophical point of view. At the same time, the dissertation attempts a partial thematic overlap, in connection with the meaning of the selected concept of responsibility, within the ethics and particularly even other related social science disciplines. In the beginning, the basic problematics is set here. This discusses the etymological point of view and responsibility is subsequently given here in the connection of the context of terms such as freedom, response or ambiguity. Furthermore, the basic relationship is presented, in the context of the phenomenon of responsibility, and responsibility is also here confronted with the present. Subsequently, the approaches and theses of an important author of the 20th century, who systematically dealt with the phenomenon of responsibility, the French author Emmanuel Levinas, are particularly emphasized. The main thesis here can be characterized as an attempt to interpret and process the concept of responsibility, especially within the context of philosophical discourse. This is done mainly against the background of the thinking of Emmanuel...
|
|
| |
|
|
Threats and opportunities in motivating senior workers in education with experience in further education.
Hájková, Jana ; Svobodová, Zuzana (advisor) ; Liška, Roman (referee)
The diploma thesis deals with the motivation of managers in the field of further education. The aim of the diploma thesis is to find out how kindergarten principals with practice perceive their own motivation to continue their education, or to identify a certain key critical period during their career as a kindergarten principal. Attention is paid to the characteristics of managers, their motivation, personal development and further education. Issues related to the burnout syndrome, possible factors causining the resigns of a manager, fundamental obstacles in motivation and critical moments of managers in their careers are also elaborated. The thesis includes qualitative research based on personal interviews with educational leaders, which took the form of semi-structured interviews. Qualitative research was chosen on the basis that appropriate methods could be used to achieve the stated aim of the thesis. A research sample was selected and specified for the qualitative research work. The research sample for this thesis was selected as the headmistresses of kindergartens who have long experience in the position, six years or more. The interviews obtained were then analyzed using the coding method. The coding was divided and identified into five main categories to correspond as closely as possible...
|
|
| |
|
|
Motivation and barriers of adult students in education at university.
Bakalíková, Gabriela ; Svobodová, Zuzana (advisor) ; Dostálová, Vladimíra (referee)
The diploma thesis deals with the motivation and barriers in education of adult students at a college. The issue of motivation and barriers of selected economically active students in higher education is discussed. The thesis is divided into a theoretical and an empirical part. The theoretical part describes the basic concepts related to the topic of the diploma thesis - motivation, motivation to learn, lifelong learning, adult education, higher education, barriers in education and more. The aim of the thesis is to find out and describe what motivated and continues to motivate selected economically active adults to study at a college, to identify the barriers they feel in this education and to find out how they deal with these barriers. The thesis identifies, analyzes and describes the motivation and barriers of selected adult students in education at a selected college. As part of the empirical part of the thesis, based on its aim, research questions are set, to which answers are subsequently sought through investigation. The empirical part contains a description of the methodology, interpretation of the collected data, a summary of the results and the outputs of the investigation. The entire research is based on qualitative methodology, specifically on the grounded theory research design. As part...
|
guest ::
