|
|
Biosensors based on modified TiO2 nanoparticles
Valkovičová, Jiřina ; Adam, Vojtěch (referee) ; Hubálek, Jaromír (advisor)
The work is focused on quantum dots (QDs) array fabrication from titanium dioxide using template based non-lithographic method and their luminescence (fluorescence) properties study. The paper describes TiO2 QDs surface modification with gold using suitable template technique and subsequent functionalization of gold modified QDs surface via oligonucleotides. This functinalization step provides a suitable base for subsequent hybridization reaction with detected nucleic acid. The system of biosensor (TiO2 QDs modified with gold and biomolecules) is designed for rapid optical diagnosis of viral diseases.
|
|
|
Isolation and purification of nucleic acids using magnetised micro- and nanoparticles
Dostálová, Simona ; Adam, Vojtěch (referee) ; Kizek, René (advisor)
Nucleic acids are a completely unique component of every organism. They determine the appearance, behaviour or disposition to a certain disease. Despite their importance, there is still a lot of information unknown. In order to study the nucleic acids, they need to be purified from other components of the organism. The routine methods of isolation and purification are often laborious and time consuming; therefore experiments with magnetic separation are carried out. This thesis deals with magnetic micro- and nanoparticles and their application in this field. General conditions of this isolation are summarized in the thesis. Detection methods of nucleic acids are listed, focusing on electrochemical methods. A procedure for isolation and purification of nucleic acids using magnetic particles and square-wave voltammetry is described. This procedure was modified in experimental part of the thesis to achieve the maximum yield. Partial steps including washing, immobilization and elution were optimized. Results showed that the phosphate buffer was optimal for washing of beads before immobilization of nucleic acids on their surface. The optimal immobilization was carried out for 5 minutes at 20 °C and microtube needed to be firmly shaken during the process. Immobilization solution composed of 0.1M Na2HPO4 + 0.1M NaH2PO4, 0.6M guanidinium thiocyanate, 0.15M Trizma base adjusted by HCl on pH 7.5 and 2.5M CsCl. 5M NaCl was optimal for washing of the complex “beads-nucleic acid” after the immobilization. Elution was carried out for 15 minutes at 99 °C and the highest yield was obtained using elution solution composed of Tris-EDTA pH at 9.1
|
|
|
Mathematical analysis in mass spectrometry
Vlachová Hutová, Eliška ; Adam, Vojtěch (referee) ; Valla, Martin (advisor)
In this bachelor work, there is theoretically described the principle of mass spectometry and tandem mass spectometry, usage the method in practices and description of functionality of separate analysers. Next, there is also mentioned the description of mass spectrum and the algorithm to its analysis. Practical part of the work describes the creation of analytic tool to evaluate spectrum and following usage of this programming tool for the practical analysis of flavonoid compounds, concretely isoflavonoid.
|
|
|
Synthesis of spherical gold nanoparticles for biomedical applications
Gablech, Evelína ; Adam, Vojtěch (referee) ; Drbohlavová, Jana (advisor)
Tato práce se zabývá syntézou sférických zlatých nanočástic pro biomedicínské aplikace. Zlaté nanočástice byly syntetizovány ekologicky nezávadnými metodami, kterým je věnována i značná část rešerše. Cílem bylo nasyntetizovat stabilní koloidní zlaté nanočástice vhodné pro různé biomedicínké aplikace zejména, pro in vivo a in vitro zobrazovací metody, kterých přehled je take obsažen v teoretické části práce. Také byl proveden test cytotoxicity, jelikož částice mají být použitelné pro in vivo aplikace. Částice byly dale charakterizovány metodami SEM, DLS a UV-VIS.
|
|
| |
|
| |
|
| |
|
|
Multiinstrumental analysis of the effect of cadmium ions on selected flax cultivars
Kryštofová, O. ; Baloun, J. ; Húska, D. ; Diopan, V. ; Shestivska, V. ; Zítka, O. ; Adam, V. ; Macek, Tomáš ; Macková, M. ; Havel, L. ; Kizek, R.
Multiinstrumental analysis of the effect of cadmium ions on selected flax cultivars was performed, revealing marked differences in synthesis of protective compounds by chromatographic, spectrometric and electrochemical techniques.
|
|
|
Metallothionenin levels in melanomas
Šobrová, P. ; Adam, V. ; Fabrik, I. ; Křížková, S. ; Kukačka, J. ; Průša, R. ; Strnádel, Ján ; Horák, Vratislav ; Kizek, R.
Level of metallothionens in various biological materials was determined by adsorptive transfer stripping differential pulse voltammetry Brdicka reaction. Human melanoma cell lines, primary skin melanomas and organ metastases of miniature pigs of the MeLiM (Melanoma-bearing Libechov Minipig) strain, and sera of patients treated for melanoma were compared. Ascertained results suggest that studies of metallothionen level are insteresting from the viewpoint of general knowledge of its relation to a course of this cancer disease. They also bring new findings needed for understanding and monitoring of therapeutic response.
|
|
|
Elektrochemické stanovení metalothioneinu v transgenním tabáku
Křížková, S. ; Diopan, V. ; Baloun, J. ; Šupálková, V. ; Shestisvka, V. ; Kotrba, P. ; Macková, Martina ; Macek, Tomáš ; Havel, L. ; Adam, V. ; Zehnálek, J. ; Kizek, R.
Inspection of CUP1 content in selected tobacco lineages using electrochemical method optimized for its selective determination was described.
|
guest ::
