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Measure the impact of wireless technologies on TEM
Prokop, Martin ; Čudek, Pavel (referee) ; Polsterová, Helena (advisor)
The purpose of this diploma thesis is to research and compare available wireless communication technologies (frequency, modulation type, transmission speed and consumption). Describe transmission electron microscopes theory and deduce the most sensitive microscope parts to high frequency distortion. Investigate effect of wireless technology on CE standards and come up with, perform and evaluate the influence measurement of chosen technologies on base parameters of transmission electron microscope.
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Methodology of Passportization of Building Objects Damaged by Surrounding Activities
Novák, Jindřich ; Hobst, Leonard (referee) ; Anton, Ondřej (advisor)
The passportization of building structures is very important task efore the initiation of negative influences on to object. The aim is to prevent possible legal deputies in between owners of affected object. The demand of passportization of building structure is increasing with the exploding of developments projects from green fields to vacancies. The aim of this work is to determine the metodology of passportization of building structures and to apply it on existig object.
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The issue of revision of the electric sliding contact machines
Kozumplík, Miroslav ; Janda, Marcel (referee) ; Veselka, František (advisor)
This work is dealing with issue about revisions of electrical equipment with focused on sliding contact. The first time I will deal enumaration and simple desription the mainly machines which uses sliding contact. The next time i will deal legislation two of standard for revision and then I will write about basic procedures for work with microscope. The next time I will bring photographic documentation of samples of brushes and at the last the strengh test of brushes with results.
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Light microscopy and its applications
Pokorný, Pavel ; Prochazka, David (referee) ; Lošťák, Martin (advisor)
This bachelor´s thesis describes problems of light microscopy for readers. Part of this bachelor´s thesis is dedicated to basic terms of the light microscopy. Next chapters are dedicated to observing techniques - brightfield illumination, darkfield illumination, oblique illumination, Rheinberg illumination, phase contrast, differential interference contrast, polarized light microscopy and fluorescence illumination.
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Microscope and it´s use in engineering practice
Kopecký, Jiří ; Dostál, Lukáš (referee) ; Veselka, František (advisor)
This work deals with the use of the light microscope in industry and its use within the automatization of the diagnostic method specialized on electrical machines with a sliding contact. The method is called the dust particles method and it is used to determine the loss of the brushes used in the machines. The current method involves a manual evaluation of an acquired data, which demands additional tests and the evaluation itself is very difficult. The objective of this work is to simplify and accelerate the method using the computer analysis. First, the usage of the light microscope, through which the samples are obtained, is showed. The method uses these samples as the input. Then, the principles of the dust particles method are demonstrated and the image analysis using the MATLAB program follows. The guide to the image analysis with the elimination of rising defects using the MATLAB program is available. Then, the volume of the intercepted dust particles is calculated using the MATLAB program. The dust particles were obtained from the LFC 55h brush using the Arsenal LPT 1513 – T light microscope.
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Design of manipulator of the sample for CCHM microscope
Špaček, Matěj ; Brada, Michal (referee) ; Dostál, Zbyněk (advisor)
In the Laboratory of the Experimental Biophotonics at Brno University of Technology, a new generation of the Coherence-Controlled Holographic Microscope (CCHM) is being developed. Because of the automation of certain microscope procedures, a fully motorized three axis computer controlled manipulator of the sample is to be employed. A fast and accurate lateral positioning in a range of several centimeters is required. For the focusing capability, very precise positioning in the optical axes is required as well. Initially, the basics of microscope manipulator design are presented. Then, commercially available solutions are introduced and compared with special focus on its possible implementation in the new generation of CCHM. However, due to the specific design of the new CCHM, available manipulators are not suitable. Therefore, a new optimized manipulator design is introduced in the second part of the thesis. A selection of its appropriate elements, especially actuators, is based on a review. For the new manipulator design, a technical documentation was made and a testing software was developed. A crucial part of the manipulator was assembled and tested.
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Measurement of the properties of cells grown in hydrogel
Belák, Daniel ; Janoušek, Oto (referee) ; Čmiel, Vratislav (advisor)
Main subject of this work is to present the possibilities of cultivation of cells in cultivation medium – hydrogel. Topic of the following part is introduction to the problematics of processing of the pictures made by light field microscopy and fluorescence microscopy. It studies the influence of parametres used in the process of stacking the pictures to reach the highest depth of field as possible. Last part is dedicated to measurement of the properties of cell cultures, its statistical processing and analysis.
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