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Matrimonial crises by clients of marriage counselling service
Rataj, Pavel ; Loneková, Katarína (advisor) ; Šulová, Lenka (referee)
This diploma work is dealing with the phenomenon of matrimonial crises. In the introductory chapters of the theoretical part we are defining the main themes of this work - relationship, philosophy of marriage, crises and marital crises. We are concerned with the couples therapy, its history and selected approaches. One chapter is devoted to the developmental approach that gives the possibility to see marital crises as an opportunity to self-knowledge, personality growth or relationship advancement. Practical part is conceived as a qualitative research. Its main goal is to survey and explore marital crises that we observed in clients of marital consulting centre. We would like to offer a more generalized vision of marital crises. We would also answer the questions how does a marital crises looks like and what processes are going on. Our work is setting down the research goals and describes in details chosen methodology, research sample and the methods of collecting and processing the data. Research data were collected mainly in the half- structured interview that was completed by the observation of interaction styles and a technique of relationship sculpturing. The research results were processed with the help of the method of open coding that is part of the grounded theory. The data were analyzed,...
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Tool for Generating Polymorphic Network Attacks
Buchta, David ; Malinka, Kamil (referee) ; Ovšonka, Daniel (advisor)
This bachelor thesis presents design and implementation of desktop GUI application for generating polymorphic network attacks. Created application implements several evasion techniques. This application also allow user to create custom techniques and use it in application. Application sends large amount of attacks in purpose to find successful NIDS evasion.
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Evaluation of properties of new HILIC stationary phases for pteridins using UHPLC-FD method
Kaufmannová, Iva ; Nováková, Lucie (advisor) ; Šatínský, Dalibor (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of analytical chemistry Candidate: Iva Kaufmannová Supervisor: PharmDr. Lucie Nováková, Ph.D. Title of Doctoral Thesis: Evaluation of properties of new HILIC stationary phases for pteridins using UHPLC-FD method The subject of this thesis was to evaluate the characteristics of three new types of HILIC stationary phases for the identification and quantification of biologically active substances biopterin, neopterin, dihydrobiopterin and dihydroneopterin by UHPLC with fluorescence detection. Neopterin is used as a marker of immune system activation and inflammatory diseases. Its early detection in urine or plasma may indicate a pathological immune activity. Elevated concentration of neopterin is described in viral or bacterial diseases, in autoimmune diseases in HIV infection or in malignant tumors. 7,8 - dihydroneopterin is able to prevent proteins and lipoproteins from oxidative damage. Hydrophilic Interaction Liquid Chromatography (HILIC) is a chromatographic method that can be used to improve retention of very polar compounds. It uses a polar stationary phase and mobile phase containing a certain amount of water and polar solvents. UHPLC is the most advanced separation technology to develop a pressure of about 100 MPa....
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Molecular evaluation of novel BCR/ABL kinase domain variants in patients with chronic myeloid leukemia
Dvořáková, Lucie ; Peková, Soňa (advisor) ; Kozák, Tomáš (referee)
1 Abstract BCR/ABL is a constitutively active tyrosine kinase that has been shown to be at the heart of the development of chronic myeloid leukemia (CML) and about 30% of acute lymphoblastic leukemia (ALL). With the recent advent of tyrosine kinase inhibitors (TKIs), exemplified by Imatinib, Nilotinib, Dasatinib and Bosutinib, patients with Ph+ CML or ALL are candidates for the therapy with these agents. From the available TKIs, Imatinib is considered as front-line therapy for CML patients in chronic phase, while for Ph+ ALL patients, 2nd generation TKIs (nilotinib, dasatinib, bosutinib) might be considered as more effective therapeutic option. Since the treatment with TKIs is a long-term affair, a substantial proportion of patients acquire some sort of mutation in kinase domain of BCR- ABL, which could be a reason of treatment failure. To date, over ninety BCR/ABL kinase domain mutations have been identified, affecting over 50 amino acids. Recurrent BCR/ABL kinase domain mutations have already been in vitro tested to approximate for their in vivo behavior. Our goal is to invent in vitro technique that would allow testing TKI sensitivity of novel BCR/ABL kinase domain mutations, identified at very low MRD levels. The technique makes use of site-directed mutagenesis to create the novel BCR/ABL kinase domain...
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Photographic album until 1914
Lesenská, Lenka ; JŮN, Libor (advisor) ; Kliment, Petr (referee)
Definition of the conservation processes, which are appropriate and inappropriate for the conservation of secession albums in the reflection of photographic techniques, which are an integral part of the album. Another aim is to define an imaginary boundaries recommending to conservator of photos, what should be able done by himself, which intervations should have been consulting with a specialist of the issue and the intervations that should be done by a specialist of the issue.
The work also contains descriptions of used materiál, their usual damage and especially the conservation techniques.
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Aroma active compounds of selected types of medical herbs
Pecinová, Ester ; Diviš, Pavel (referee) ; Vítová, Eva (advisor)
This thesis deals with aroma active compounds of selected types of medicinal herbs, specifically lavender (Lavandula officinalis), peppermint (Mentha piperita), sage (Salvia officinalis), St. John’s wort (Hypericum perforatum) and hyssop (Hyssopus officinalis). In the theoretical part the single herbs were characterized including botanical description, chemical composition, medicinal properties, adverse effects and the practical use; aroma active compounds present in herbs are also emphasized. Based on the literature search, gas chromatography coupled with mass spectrometry as currently the most frequently used method, was chosen for the assessment of aroma compounds. Solid Phase Microextraction was used for isolation of volatile compounds from dried herbs. Within experimental part of the work selected parameters of the SPME-GC-MS method were optimized, aroma compounds in samples of herbs were identified, and twenty four suspected fragrance allergens due to the Regulation (EC) N° 1223/2009 were also identified and quantified. Overall 69 compounds were indetified together with 2 allergens in lavender, 59 compounds including 5 allergens in peppermint, sage contained 49 compounds with 4 allergens included, 41 compounds were identified in St. John’s wort including 4 allergens and finally 64 compounds with 4 allergens included in hyssop.
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The application of magnetic nano- and microparticles for the isolation of DNA from selected foods
Ráčková, Lucie ; Rittich, Bohuslav (referee) ; Kovařík, Aleš (advisor)
In thesis was verified micromethod for isolation of plant DNA from different vegetable (onion and broccoli) and plant food products in quality for application in polymerase chain reaction (PCR). The micromethod allows isolation DNA using magnetic particles from crude lysates of cells obtained by direct homogenization of plant tissues. Various methods of processing homogenates were compared. Homogenization was performed by lysis buffer containing cetyltrimethylammonium bromide (CTAB). The effect of the organic extraction agents was tested (chloroform-octanol and isopropanol). DNA was purified from homogenates by reversible adsorption on magnetic particles (four different types of magnetic particles were tested). The quality of isolated DNA was verified by UV spectrophotometry. The amplificabilty of DNA was tested by polymerase chain reaction (PCR). Specific primers for plant ribosomal DNA (rDNA) were used. PCR products of lenght 700 and 220 bp were detected by agarose gel electrophoresis. Differences in yield and quality of DNA were depended on the homogenate processing and magnetic particles used. The proposed procedure with two magnetic particles was tested for the isolation DNA from plan food products (spreads). DNA was amplified in PCR. Micromethod is suitable for DNA analysis of foods.
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PROBLEMS OF PATIENT RESOCIALIZATION IN PSYCHIATRIC HOSPITAL ČERVENÝ DVŮR
DVOŘÁKOVÁ, Lucie
This bachelor thesis called The Issues of Rehabilitation of the Psychiatric Hospital Cerveny Dvur patients deals with both the rehabilitation of the Cerveny Dvur patients and system of the rehabilitation care provided after the stay in this hospital. The thesis is divided into two parts, a theoretical and practical one. The theoretical part contains basic concepts concerning the issues of rehabilitation, specific problems during the rehabilitation which can appear after the return to a normal life, the issues of relapse or recurrence and finally the information about the psychiatric hospital Cerveny Dvur where the research was realized. The main goal of the practical part was to chart the rehabilition of the Cerveny Dvur patients with the emphasis on sub-goals such as the system of the care provided after the stay in this hospital as well as the reasons for the abstinence violation. There were three research issues defined which were focused on the functioning of the aftercare, risk factors during the rehabilitation and the most frequent reasons for the abstinence violation. The research itself was carried out qualitatively since the data were obtained by the method of semi-structured interviewing. Seven patients and three therapists of Cerveny Dvur took part in it and the research was realized just at this hospital. During the discussion I was comparing the results of this research with professional publications. Finally I establish hypothesis and give some recommendations to solve the problems resulting from the research. I think that it is necessary to devote more time to these issues and also not to underestimate the aftercare.
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